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Protein

Ribosomal RNA small subunit methyltransferase A

Gene

rsmA

Organism
Coxiella burnetii (strain RSA 493 / Nine Mile phase I)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits.UniRule annotation

Catalytic activityi

4 S-adenosyl-L-methionine + adenine(1518)/adenine(1519) in 16S rRNA = 4 S-adenosyl-L-homocysteine + N(6)-dimethyladenine(1518)/N(6)-dimethyladenine(1519) in 16S rRNA.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei13S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1
Binding sitei15S-adenosyl-L-methionine; via amide nitrogenUniRule annotation1
Binding sitei40S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1
Binding sitei61S-adenosyl-L-methionineUniRule annotation1
Binding sitei86S-adenosyl-L-methionineUniRule annotation1
Binding sitei106S-adenosyl-L-methionineUniRule annotation1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

rRNA processing

Keywords - Ligandi

RNA-binding, S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosomal RNA small subunit methyltransferase AUniRule annotation (EC:2.1.1.182UniRule annotation)
Alternative name(s):
16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferaseUniRule annotation
16S rRNA dimethyladenosine transferaseUniRule annotation
16S rRNA dimethylaseUniRule annotation
S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferaseUniRule annotation
Gene namesi
Name:rsmAUniRule annotation
Synonyms:ksgAUniRule annotation
Ordered Locus Names:CBU_1982
OrganismiCoxiella burnetii (strain RSA 493 / Nine Mile phase I)
Taxonomic identifieri227377 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaLegionellalesCoxiellaceaeCoxiella
Proteomesi
  • UP000002671 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001015201 – 258Ribosomal RNA small subunit methyltransferase AAdd BLAST258

Proteomic databases

PRIDEiQ83AC2.

Interactioni

Protein-protein interaction databases

STRINGi227377.CBU_1982.

Structurei

Secondary structure

1258
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi18 – 28Combined sources11
Beta strandi35 – 39Combined sources5
Turni42 – 46Combined sources5
Helixi47 – 50Combined sources4
Turni51 – 53Combined sources3
Beta strandi54 – 61Combined sources8
Helixi64 – 74Combined sources11
Beta strandi80 – 85Combined sources6
Turni87 – 89Combined sources3
Helixi92 – 94Combined sources3
Beta strandi101 – 106Combined sources6
Helixi109 – 121Combined sources13
Helixi122 – 125Combined sources4
Beta strandi126 – 134Combined sources9
Helixi135 – 141Combined sources7
Helixi152 – 160Combined sources9
Beta strandi161 – 169Combined sources9
Helixi171 – 173Combined sources3
Beta strandi174 – 176Combined sources3
Beta strandi182 – 189Combined sources8
Helixi200 – 212Combined sources13
Helixi218 – 221Combined sources4
Turni222 – 225Combined sources4
Helixi228 – 230Combined sources3
Helixi232 – 234Combined sources3
Helixi242 – 244Combined sources3
Helixi247 – 257Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3TQSX-ray1.98A/B/C/D4-258[»]
ProteinModelPortaliQ83AC2.
SMRiQ83AC2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105D1X. Bacteria.
COG0030. LUCA.
HOGENOMiHOG000227962.
KOiK02528.
OMAiPHPAKDH.

Family and domain databases

Gene3Di1.10.8.100. 1 hit.
3.40.50.150. 1 hit.
HAMAPiMF_00607. 16SrRNA_methyltr_A. 1 hit.
InterProiIPR001737. KsgA/Erm.
IPR023165. rRNA_Ade_diMease-like.
IPR020596. rRNA_Ade_Mease_Trfase_CS.
IPR020598. rRNA_Ade_methylase_Trfase_N.
IPR011530. rRNA_adenine_dimethylase.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR11727. PTHR11727. 1 hit.
PfamiPF00398. RrnaAD. 1 hit.
[Graphical view]
SMARTiSM00650. rADc. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00755. ksgA. 1 hit.
PROSITEiPS01131. RRNA_A_DIMETH. 1 hit.
PS51689. SAM_RNA_A_N6_MT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q83AC2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKMPMRKRF GQHFLHDSFV LQKIVSAIHP QKTDTLVEIG PGRGALTDYL
60 70 80 90 100
LTECDNLALV EIDRDLVAFL QKKYNQQKNI TIYQNDALQF DFSSVKTDKP
110 120 130 140 150
LRVVGNLPYN ISTPLLFHLF SQIHCIEDMH FMLQKEVVRR ITAEVGSHDY
160 170 180 190 200
GRLSVMAQYF CDNTYLFTVS PQAFTPPPRV ESAIIRLIPR HNFTPVAKNL
210 220 230 240 250
DQLSHVVKEA FSYRRKTVGN ALKKLINPSQ WPLLEINPQL RPQELTVEDF

VKISNILN
Length:258
Mass (Da):29,779
Last modified:December 16, 2008 - v2
Checksum:i40C0F96B085DFD2A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016828 Genomic DNA. Translation: AAO91471.2.
RefSeqiNP_820957.2. NC_002971.3.
WP_005772431.1. NZ_CCYB01000065.1.

Genome annotation databases

EnsemblBacteriaiAAO91471; AAO91471; CBU_1982.
GeneIDi1209895.
KEGGicbu:CBU_1982.
PATRICi17932705. VBICoxBur82552_1969.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016828 Genomic DNA. Translation: AAO91471.2.
RefSeqiNP_820957.2. NC_002971.3.
WP_005772431.1. NZ_CCYB01000065.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3TQSX-ray1.98A/B/C/D4-258[»]
ProteinModelPortaliQ83AC2.
SMRiQ83AC2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi227377.CBU_1982.

Proteomic databases

PRIDEiQ83AC2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAO91471; AAO91471; CBU_1982.
GeneIDi1209895.
KEGGicbu:CBU_1982.
PATRICi17932705. VBICoxBur82552_1969.

Phylogenomic databases

eggNOGiENOG4105D1X. Bacteria.
COG0030. LUCA.
HOGENOMiHOG000227962.
KOiK02528.
OMAiPHPAKDH.

Family and domain databases

Gene3Di1.10.8.100. 1 hit.
3.40.50.150. 1 hit.
HAMAPiMF_00607. 16SrRNA_methyltr_A. 1 hit.
InterProiIPR001737. KsgA/Erm.
IPR023165. rRNA_Ade_diMease-like.
IPR020596. rRNA_Ade_Mease_Trfase_CS.
IPR020598. rRNA_Ade_methylase_Trfase_N.
IPR011530. rRNA_adenine_dimethylase.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR11727. PTHR11727. 1 hit.
PfamiPF00398. RrnaAD. 1 hit.
[Graphical view]
SMARTiSM00650. rADc. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00755. ksgA. 1 hit.
PROSITEiPS01131. RRNA_A_DIMETH. 1 hit.
PS51689. SAM_RNA_A_N6_MT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRSMA_COXBU
AccessioniPrimary (citable) accession number: Q83AC2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 4, 2003
Last sequence update: December 16, 2008
Last modified: November 2, 2016
This is version 91 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Coxiella burnetii
    Coxiella burnetii (strain RSA 493): entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.