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Q83AC2 (RSMA_COXBU) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 75. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Ribosomal RNA small subunit methyltransferase A

EC=2.1.1.182
Alternative name(s):
16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase
16S rRNA dimethyladenosine transferase
16S rRNA dimethylase
S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase
Gene names
Name:rsmA
Synonyms:ksgA
Ordered Locus Names:CBU_1982
OrganismCoxiella burnetii (strain RSA 493 / Nine Mile phase I) [Reference proteome] [HAMAP]
Taxonomic identifier227377 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaLegionellalesCoxiellaceaeCoxiella

Protein attributes

Sequence length258 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits By similarity. HAMAP-Rule MF_00607

Catalytic activity

4 S-adenosyl-L-methionine + adenine(1518)/adenine(1519) in 16S rRNA = 4 S-adenosyl-L-homocysteine + N(6)-dimethyladenine(1518)/N(6)-dimethyladenine(1519) in 16S rRNA. HAMAP-Rule MF_00607

Subcellular location

Cytoplasm Potential HAMAP-Rule MF_00607.

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 258258Ribosomal RNA small subunit methyltransferase A HAMAP-Rule MF_00607
PRO_0000101520

Sites

Binding site131S-adenosyl-L-methionine; via carbonyl oxygen By similarity
Binding site151S-adenosyl-L-methionine; via amide nitrogen By similarity
Binding site401S-adenosyl-L-methionine; via carbonyl oxygen By similarity
Binding site611S-adenosyl-L-methionine By similarity
Binding site861S-adenosyl-L-methionine By similarity
Binding site1061S-adenosyl-L-methionine By similarity

Secondary structure

.............................................. 258
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q83AC2 [UniParc].

Last modified December 16, 2008. Version 2.
Checksum: 40C0F96B085DFD2A

FASTA25829,779
        10         20         30         40         50         60 
MKKMPMRKRF GQHFLHDSFV LQKIVSAIHP QKTDTLVEIG PGRGALTDYL LTECDNLALV 

        70         80         90        100        110        120 
EIDRDLVAFL QKKYNQQKNI TIYQNDALQF DFSSVKTDKP LRVVGNLPYN ISTPLLFHLF 

       130        140        150        160        170        180 
SQIHCIEDMH FMLQKEVVRR ITAEVGSHDY GRLSVMAQYF CDNTYLFTVS PQAFTPPPRV 

       190        200        210        220        230        240 
ESAIIRLIPR HNFTPVAKNL DQLSHVVKEA FSYRRKTVGN ALKKLINPSQ WPLLEINPQL 

       250 
RPQELTVEDF VKISNILN 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE016828 Genomic DNA. Translation: AAO91471.2.
RefSeqNP_820957.2. NC_002971.3.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3TQSX-ray1.98A/B/C/D4-258[»]
ProteinModelPortalQ83AC2.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING227377.CBU_1982.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAO91471; AAO91471; CBU_1982.
GeneID1209895.
KEGGcbu:CBU_1982.
PATRIC17932705. VBICoxBur82552_1969.

Phylogenomic databases

eggNOGCOG0030.
HOGENOMHOG000227962.
KOK02528.
OMADHVMVEI.
OrthoDBEOG66F08Z.

Enzyme and pathway databases

BioCycCBUR227377:GJ7S-1955-MONOMER.

Family and domain databases

Gene3D1.10.8.100. 1 hit.
3.40.50.150. 1 hit.
HAMAPMF_00607. 16SrRNA_methyltr_A.
InterProIPR023165. rRNA_Ade_diMease-like.
IPR020596. rRNA_Ade_Mease_Trfase_CS.
IPR001737. rRNA_Ade_methylase_transferase.
IPR020598. rRNA_Ade_methylase_Trfase_N.
IPR011530. rRNA_adenine_dimethylase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PANTHERPTHR11727. PTHR11727. 1 hit.
PfamPF00398. RrnaAD. 1 hit.
[Graphical view]
SMARTSM00650. rADc. 1 hit.
[Graphical view]
SUPFAMSSF53335. SSF53335. 1 hit.
TIGRFAMsTIGR00755. ksgA. 1 hit.
PROSITEPS01131. RRNA_A_DIMETH. 1 hit.
PS51689. SAM_RNA_A_N6_MT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRSMA_COXBU
AccessionPrimary (citable) accession number: Q83AC2
Entry history
Integrated into UniProtKB/Swiss-Prot: August 4, 2003
Last sequence update: December 16, 2008
Last modified: June 11, 2014
This is version 75 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Coxiella burnetii

Coxiella burnetii (strain RSA 493): entries and gene names