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Q83AA5 (Q83AA5_COXBU) Unreviewed, UniProtKB/TrEMBL

Last modified December 14, 2011. Version 69. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
DNA topoisomerase RuleBase RU000518
EC=5.99.1.2 RuleBase RU000518
Gene names
Name:topA EMBL AAO91489.1
Ordered Locus Names:CBU_2000
OrganismCoxiella burnetii
Taxonomic identifier777 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Legionellales › Coxiellaceae › Coxiella

Protein attributes

Sequence length765 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. SAAS SAAS000380

Catalytic activity

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining. RuleBase RU000518 SAAS SAAS005733

Sequence similarities

Belongs to the prokaryotic type I/III topoisomerase family. RuleBase RU004206

Contains 1 Toprim domain. SAAS SAAS023406

Sequences

Sequence LengthMass (Da)Tools
Q83AA5 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: 71E1D2C553C2AAD4

FASTA76587,206
        10         20         30         40         50         60 
MVKSLVVVES PAKAKTIEKY LGKDFKVMAS YGHVRDLIPK EGAVDPEDHF KMHYQAIERN 

        70         80         90        100        110        120 
IRHMDAIAKA LKPCHNLYLA TDPDREGEAI AWHVKQMLES RNGLKDKKFS RVVFHQITKK 

       130        140        150        160        170        180 
AVQHAIENPR DISMDLVNAQ QARRALDYLV GFNLSPLLWK KIRPGLSAGR VQSPALRLIV 

       190        200        210        220        230        240 
EREIEIENFE SQEYWTIHAD CHAQQQPFDA RLIEFQGEKL EQFSIVNEKQ AHETRDAITQ 

       250        260        270        280        290        300 
AANGKLTVAK VVKRERKRNP APPFITSTMQ QEAARKLGFS ASKTMQIAQQ LYEGVNLGEE 

       310        320        330        340        350        360 
GAMGLITYMR TDSVTLASEA LQEIRQLIEE KYGQENVPEQ TRIYKTKSKN AQEAHEAIRP 

       370        380        390        400        410        420 
TSAFRTPENL KKYLNSDQLK LYTLIWKRTI ACQMIEATLH TVAVDLKAPN AIFRANGSTI 

       430        440        450        460        470        480 
VKPGFISVYQ ETFDDKKKEE ESDSKMLPPL EEGQTVDVNE IKSEQHFTEP PPRYTEASLV 

       490        500        510        520        530        540 
KALEEYDIGR PSTYASIIAT LKNRKYVDVD NKRFIATDVG RIVNRFLTHY FTKYVDYGFT 

       550        560        570        580        590        600 
AKLEDELDDI ARGEKTWIPV LETFWSPFKN LVDVTEKTVK RSDVTQEKID EKCPKCSAQL 

       610        620        630        640        650        660 
SIRLGRRGRF IGCTAYPDCD YTRNLTEIAA EAQAVLEGRQ CPECQSNLII KMGRYGKFIG 

       670        680        690        700        710        720 
CSGYPNCKYM EPLEKPKDTN VTCPKCKKGT ILQRKSRRGK IFYSCARYPD CDYALWNEPV 

       730        740        750        760 
AEACPKCGWP ILSIKTTKSR GTEKVCPQKS CNFIEAYDGD KPSLD

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE016828 Genomic DNA. Translation: AAO91489.1.
RefSeqNP_820975.1. NC_002971.3.

3D structure databases

HSSPHSSP built from PDB template 1ECL based on UniProtKB P06612.
ProteinModelPortalQ83AA5.
ModBaseSearch...

Proteomic databases

PRIDEQ83AA5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID1209913.
GenomeReviewsGene locus CBU_2000 in contig AE016828_GR.
KEGGcbu:CBU_2000.
NMPDRfig|227377.1.peg.1919.
PATRIC17932741. VBICoxBur82552_1987.
TIGRCBU_2000.

Phylogenomic databases

HOGENOMHBG512935.
OMAIERNIRH.
PhylomeDBQ83AA5.
ProtClustDBPRK06599.

Family and domain databases

InterProIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013825. Topo_IA_cen_sub2.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd.
IPR013498. Topo_IA_Znf.
IPR005733. TopoI_bac-type.
IPR006171. Toprim_domain.
[Graphical view]
Gene3DG3DSA:1.10.460.10. Topo_IA_cen_sub1. 2 hits.
G3DSA:2.70.20.10. Topo_IA_cen_sub2. 2 hits.
KOK03168.
PANTHERPTHR11390. Topo_IA. 1 hit.
PfamPF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
PF01396. zf-C4_Topoisom. 3 hits.
[Graphical view]
PRINTSPR00417. PRTPISMRASEI.
SMARTSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMSSF56712. Topo_IA_core. 1 hit.
TIGRFAMsTIGR01051. TopA_bact. 1 hit.
PROSITEPS00396. TOPOISOMERASE_I_PROK. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameQ83AA5_COXBU
AccessionPrimary (citable) accession number: Q83AA5
Entry history
Integrated into UniProtKB/TrEMBL: June 1, 2003
Last sequence update: June 1, 2003
Last modified: December 14, 2011
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequences·References·Cross-refs·Entry info