Reviewed, UniProtKB/Swiss-Prot Q83A83 (METC_COXBU)

Names and origin

Protein names

Recommended name:
    Cystathionine beta-lyase
      Short name=CBL
    EC=4.4.1.8
Alternative name(s):
    Beta-cystathionase
    Cysteine lyase

Gene names

Name:	metC
Ordered Locus Names:	CBU_2025

Organism

Coxiella burnetii [Complete proteome] [HAMAP]

Taxonomic identifier

777 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Legionellales › Coxiellaceae › Coxiella

Protein attributes

Sequence length	387 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	L-cystathionine + H2O = L-homocysteine + NH3 + pyruvate.
Cofactor	Pyridoxal phosphate By similarity.
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homocysteine from L-cystathionine: step 1/1.
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm By similarity.
Developmental stage	More than twofold more abundant in the small cell variant (SCV) stage than in the large cell variant (LCV) stage (at protein level). LCVs are more metabolically active than SCVs. Ref.2
Sequence similarities	Belongs to the trans-sulfuration enzymes family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Cellular component	Cytoplasm
Ligand	Pyridoxal phosphate
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cystathionine beta-lyase activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 387	387	Cystathionine beta-lyase		PRO_0000320579
Amino acid modifications
	Modified residue	204	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q83A83-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 6DF7959AA345B6AB	FASTA	387	42,776
	10 20 30 40 50 60 MTANNNKKSH IDTRVIHAGQ KPDPLTGAVM TPIYTASTYA QKSPGVHQGY EYSRSQNPTR 70 80 90 100 110 120 FAYERCVADL ESGQHGFAFA SGMAATATIL ELLQPGDHVV VMDDVYGGSY RLFENVRKRS 130 140 150 160 170 180 AGLSFSFVDF TDENKVREAV TAKTKMLWVE SPSNPRLKIV DLAKIAEIAK EKNIIAVADN 190 200 210 220 230 240 TFATPIIQRP LELGFDIVTH SATKYLNGHS DIIGGVAVVG DNKTLAEQLK YLQNAIGAIA 250 260 270 280 290 300 APFDSFMVLR GLKTLAIRME RHCENAMQLA QWLEKHPKVK RVYYPGLPSH PQHSIAKKQM 310 320 330 340 350 360 RYFGGMISVE LKCDLNETKK VLERCQLFTL AESLGGVESL IEHPAIMTHA SIPQAERQKL 370 380 GITDGFIRLS VGIEAITDLR HDLEAAL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete genome sequence of the Q-fever pathogen, Coxiella burnetii." Seshadri R., Paulsen I.T., Eisen J.A., Read T.D., Nelson K.E., Nelson W.C., Ward N.L., Tettelin H., Davidsen T.M., Beanan M.J., DeBoy R.T., Daugherty S.C., Brinkac L.M., Madupu R., Dodson R.J., Khouri H.M., Lee K.H., Carty H.A. , Scanlan D., Heinzen R.A., Thompson H.A., Samuel J.E., Fraser C.M., Heidelberg J.F. Proc. Natl. Acad. Sci. U.S.A. 100:5455-5460(2003) [PubMed: 12704232] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Nine Mile phase I / RSA 493.
[2]	"Proteome and antigen profiling of Coxiella burnetii developmental forms." Coleman S.A., Fischer E.R., Cockrell D.C., Voth D.E., Howe D., Mead D.J., Samuel J.E., Heinzen R.A. Infect. Immun. 75:290-298(2007) [PubMed: 17088354] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, DEVELOPMENTAL STAGE. Strain: Nine Mile Crazy / RSA 514.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016828 Genomic DNA. Translation: AAO91512.1.
RefSeq	NP_820998.1.
3D structure databases
HSSP	HSSP built from PDB template 1GC0 based on UniProtKB P13254.
SMR	Q83A83. Positions 6-387.
ModBase	Search...
Genome annotation databases
GeneID	1209938.
GenomeReviews	Gene locus CBU_2025 in contig AE016828_GR.
KEGG	cbu:CBU_2025.
NMPDR	fig|227377.1.peg.1942.
TIGR	CBU_2025.
Phylogenomic databases
HOGENOM	HBG754729.
OMA	NQYEYSR.
PhylomeDB	Q83A83.
Enzyme and pathway databases
BioCyc	CBUR227377:CBU_2025-MONOMER.
BRENDA	4.4.1.8. 256353.
Family and domain databases
InterPro	IPR000277. Cys/Met-Metab_PyrdxlP-dep_enz. IPR015424. PyrdxlP-dep_Trfase_major_dom. IPR015421. PyrdxlP-dep_Trfase_major_sub1. IPR015422. PyrdxlP-dep_Trfase_major_sub2. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit. G3DSA:3.90.1150.10. PyrdxlP-dep_Trfase_major_sub2. 1 hit.
PANTHER	PTHR11808. Cys_Met_Meta_PP. 1 hit.
Pfam	PF01053. Cys_Met_Meta_PP. 1 hit. [Graphical view]
PIRSF	PIRSF001434. CGS. 1 hit.
PROSITE	PS00868. CYS_MET_METAB_PP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

METC_COXBU

Accession

Primary (citable) accession number: Q83A83

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	June 1, 2003
Last modified:	January 19, 2010
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)