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Q83A78 (METK_COXBU) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 78. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
S-adenosylmethionine synthase

Short name=AdoMet synthase
EC=2.5.1.6
Alternative name(s):
MAT
Methionine adenosyltransferase
Gene names
Name:metK
Ordered Locus Names:CBU_2030
OrganismCoxiella burnetii (strain RSA 493 / Nine Mile phase I) [Reference proteome] [HAMAP]
Taxonomic identifier227377 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaLegionellalesCoxiellaceaeCoxiella

Protein attributes

Sequence length393 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the enzyme By similarity. HAMAP-Rule MF_00086

Catalytic activity

ATP + L-methionine + H2O = phosphate + diphosphate + S-adenosyl-L-methionine. HAMAP-Rule MF_00086

Cofactor

Binds 2 divalent ions per subunit. Magnesium or cobalt By similarity.

Binds 1 potassium ion per subunit By similarity. HAMAP-Rule MF_00086

Pathway

Amino-acid biosynthesis; S-adenosyl-L-methionine biosynthesis; S-adenosyl-L-methionine from L-methionine: step 1/1. HAMAP-Rule MF_00086

Subunit structure

Homotetramer By similarity. HAMAP-Rule MF_00086

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00086.

Sequence similarities

Belongs to the AdoMet synthase family.

Ontologies

Keywords
   Biological processOne-carbon metabolism
   Cellular componentCytoplasm
   LigandATP-binding
Cobalt
Magnesium
Metal-binding
Nucleotide-binding
Potassium
   Molecular functionTransferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processS-adenosylmethionine biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

one-carbon metabolic process

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

magnesium ion binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

methionine adenosyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 393393S-adenosylmethionine synthase HAMAP-Rule MF_00086
PRO_0000174514

Regions

Nucleotide binding261 – 2688ATP Potential

Sites

Metal binding181Magnesium By similarity
Metal binding441Potassium By similarity
Metal binding2651Potassium By similarity
Metal binding2731Magnesium By similarity

Sequences

Sequence LengthMass (Da)Tools
Q83A78 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: 9AFBE2DA6A03929D

FASTA39342,777
        10         20         30         40         50         60 
MTHTTLFTSE SVSEGHPDKV ADQISDAVLD ALIGQDPNCR VACEAVVKSG MVFVAGEITT 

        70         80         90        100        110        120 
NAWADVEQIT RNTVLQIGYN DPHLGFDGET CAVVTAIGKQ SPDIVMGVDG REDQELGAGD 

       130        140        150        160        170        180 
QGLMFGYASR ETNVFMPAPI TYAHRLVRQQ ALLRKNGRLP WLRPDAKSQV TLRYENGKPV 

       190        200        210        220        230        240 
AADCIVLSTQ HSPEISQESL REAVIDEIIK PIMPPHWLDK HTRFLVNPTG RFVIGGPVGD 

       250        260        270        280        290        300 
CGLTGRKIIV DTYGGMARHG GGCFSGKDPS KVDRSGAYAA RYVAKNIVAG GLADRCEIQV 

       310        320        330        340        350        360 
SYAIGVAEPT SISVETFGTG KIDEVRIQQL IKEHFDLRPG KIIEELNLLR SIYKATATYG 

       370        380        390 
HFGREEPEFT WERTDKAEIL REAAGLAAAN VTN 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE016828 Genomic DNA. Translation: AAO91517.1.
RefSeqNP_821003.1. NC_002971.3.

3D structure databases

ProteinModelPortalQ83A78.
SMRQ83A78. Positions 6-386.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING227377.CBU_2030.

Proteomic databases

PRIDEQ83A78.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAO91517; AAO91517; CBU_2030.
GeneID1209943.
KEGGcbu:CBU_2030.
PATRIC17932813. VBICoxBur82552_2023.

Phylogenomic databases

eggNOGCOG0192.
HOGENOMHOG000245710.
KOK00789.
OMAIYYAHRL.
OrthoDBEOG68WR6M.
ProtClustDBPRK05250.

Enzyme and pathway databases

BioCycCBUR227377:GJ7S-2004-MONOMER.
UniPathwayUPA00315; UER00080.

Family and domain databases

HAMAPMF_00086. S_AdoMet_synth1.
InterProIPR022631. ADOMET_SYNTHASE_CS.
IPR022630. S-AdoMet_synt_C.
IPR022629. S-AdoMet_synt_central.
IPR022628. S-AdoMet_synt_N.
IPR002133. S-AdoMet_synthetase.
IPR022636. S-AdoMet_synthetase_sfam.
[Graphical view]
PANTHERPTHR11964. PTHR11964. 1 hit.
PfamPF02773. S-AdoMet_synt_C. 1 hit.
PF02772. S-AdoMet_synt_M. 1 hit.
PF00438. S-AdoMet_synt_N. 1 hit.
[Graphical view]
PIRSFPIRSF000497. MAT. 1 hit.
SUPFAMSSF55973. SSF55973. 3 hits.
TIGRFAMsTIGR01034. metK. 1 hit.
PROSITEPS00376. ADOMET_SYNTHASE_1. 1 hit.
PS00377. ADOMET_SYNTHASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMETK_COXBU
AccessionPrimary (citable) accession number: Q83A78
Entry history
Integrated into UniProtKB/Swiss-Prot: July 25, 2003
Last sequence update: June 1, 2003
Last modified: February 19, 2014
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Coxiella burnetii

Coxiella burnetii (strain RSA 493): entries and gene names