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Q83A78

- METK_COXBU

UniProt

Q83A78 - METK_COXBU

Protein

S-adenosylmethionine synthase

Gene

metK

Organism
Coxiella burnetii (strain RSA 493 / Nine Mile phase I)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 80 (01 Oct 2014)
      Sequence version 1 (01 Jun 2003)
      Previous versions | rss
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    Functioni

    Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the enzyme.UniRule annotation

    Catalytic activityi

    ATP + L-methionine + H2O = phosphate + diphosphate + S-adenosyl-L-methionine.UniRule annotation

    Cofactori

    Binds 2 divalent ions per subunit. Magnesium or cobalt.UniRule annotation
    Binds 1 potassium ion per subunit.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi18 – 181MagnesiumUniRule annotation
    Metal bindingi44 – 441PotassiumUniRule annotation
    Metal bindingi265 – 2651PotassiumUniRule annotation
    Metal bindingi273 – 2731MagnesiumUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi261 – 2688ATPUniRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. magnesium ion binding Source: UniProtKB-HAMAP
    3. methionine adenosyltransferase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. one-carbon metabolic process Source: UniProtKB-HAMAP
    2. S-adenosylmethionine biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    One-carbon metabolism

    Keywords - Ligandi

    ATP-binding, Cobalt, Magnesium, Metal-binding, Nucleotide-binding, Potassium

    Enzyme and pathway databases

    BioCyciCBUR227377:GJ7S-2004-MONOMER.
    UniPathwayiUPA00315; UER00080.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    S-adenosylmethionine synthaseUniRule annotation (EC:2.5.1.6UniRule annotation)
    Short name:
    AdoMet synthaseUniRule annotation
    Alternative name(s):
    MATUniRule annotation
    Methionine adenosyltransferaseUniRule annotation
    Gene namesi
    Name:metKUniRule annotation
    Ordered Locus Names:CBU_2030
    OrganismiCoxiella burnetii (strain RSA 493 / Nine Mile phase I)
    Taxonomic identifieri227377 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaLegionellalesCoxiellaceaeCoxiella
    ProteomesiUP000002671: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 393393S-adenosylmethionine synthasePRO_0000174514Add
    BLAST

    Proteomic databases

    PRIDEiQ83A78.

    Interactioni

    Subunit structurei

    Homotetramer.UniRule annotation

    Protein-protein interaction databases

    STRINGi227377.CBU_2030.

    Structurei

    3D structure databases

    ProteinModelPortaliQ83A78.
    SMRiQ83A78. Positions 6-386.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the AdoMet synthase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0192.
    HOGENOMiHOG000245710.
    KOiK00789.
    OMAiGYVNSEM.
    OrthoDBiEOG68WR6M.

    Family and domain databases

    HAMAPiMF_00086. S_AdoMet_synth1.
    InterProiIPR022631. ADOMET_SYNTHASE_CS.
    IPR022630. S-AdoMet_synt_C.
    IPR022629. S-AdoMet_synt_central.
    IPR022628. S-AdoMet_synt_N.
    IPR002133. S-AdoMet_synthetase.
    IPR022636. S-AdoMet_synthetase_sfam.
    [Graphical view]
    PANTHERiPTHR11964. PTHR11964. 1 hit.
    PfamiPF02773. S-AdoMet_synt_C. 1 hit.
    PF02772. S-AdoMet_synt_M. 1 hit.
    PF00438. S-AdoMet_synt_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000497. MAT. 1 hit.
    SUPFAMiSSF55973. SSF55973. 3 hits.
    TIGRFAMsiTIGR01034. metK. 1 hit.
    PROSITEiPS00376. ADOMET_SYNTHASE_1. 1 hit.
    PS00377. ADOMET_SYNTHASE_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q83A78-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTHTTLFTSE SVSEGHPDKV ADQISDAVLD ALIGQDPNCR VACEAVVKSG    50
    MVFVAGEITT NAWADVEQIT RNTVLQIGYN DPHLGFDGET CAVVTAIGKQ 100
    SPDIVMGVDG REDQELGAGD QGLMFGYASR ETNVFMPAPI TYAHRLVRQQ 150
    ALLRKNGRLP WLRPDAKSQV TLRYENGKPV AADCIVLSTQ HSPEISQESL 200
    REAVIDEIIK PIMPPHWLDK HTRFLVNPTG RFVIGGPVGD CGLTGRKIIV 250
    DTYGGMARHG GGCFSGKDPS KVDRSGAYAA RYVAKNIVAG GLADRCEIQV 300
    SYAIGVAEPT SISVETFGTG KIDEVRIQQL IKEHFDLRPG KIIEELNLLR 350
    SIYKATATYG HFGREEPEFT WERTDKAEIL REAAGLAAAN VTN 393
    Length:393
    Mass (Da):42,777
    Last modified:June 1, 2003 - v1
    Checksum:i9AFBE2DA6A03929D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE016828 Genomic DNA. Translation: AAO91517.1.
    RefSeqiNP_821003.1. NC_002971.3.

    Genome annotation databases

    EnsemblBacteriaiAAO91517; AAO91517; CBU_2030.
    GeneIDi1209943.
    KEGGicbu:CBU_2030.
    PATRICi17932813. VBICoxBur82552_2023.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE016828 Genomic DNA. Translation: AAO91517.1 .
    RefSeqi NP_821003.1. NC_002971.3.

    3D structure databases

    ProteinModelPortali Q83A78.
    SMRi Q83A78. Positions 6-386.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 227377.CBU_2030.

    Proteomic databases

    PRIDEi Q83A78.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAO91517 ; AAO91517 ; CBU_2030 .
    GeneIDi 1209943.
    KEGGi cbu:CBU_2030.
    PATRICi 17932813. VBICoxBur82552_2023.

    Phylogenomic databases

    eggNOGi COG0192.
    HOGENOMi HOG000245710.
    KOi K00789.
    OMAi GYVNSEM.
    OrthoDBi EOG68WR6M.

    Enzyme and pathway databases

    UniPathwayi UPA00315 ; UER00080 .
    BioCyci CBUR227377:GJ7S-2004-MONOMER.

    Family and domain databases

    HAMAPi MF_00086. S_AdoMet_synth1.
    InterProi IPR022631. ADOMET_SYNTHASE_CS.
    IPR022630. S-AdoMet_synt_C.
    IPR022629. S-AdoMet_synt_central.
    IPR022628. S-AdoMet_synt_N.
    IPR002133. S-AdoMet_synthetase.
    IPR022636. S-AdoMet_synthetase_sfam.
    [Graphical view ]
    PANTHERi PTHR11964. PTHR11964. 1 hit.
    Pfami PF02773. S-AdoMet_synt_C. 1 hit.
    PF02772. S-AdoMet_synt_M. 1 hit.
    PF00438. S-AdoMet_synt_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000497. MAT. 1 hit.
    SUPFAMi SSF55973. SSF55973. 3 hits.
    TIGRFAMsi TIGR01034. metK. 1 hit.
    PROSITEi PS00376. ADOMET_SYNTHASE_1. 1 hit.
    PS00377. ADOMET_SYNTHASE_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: RSA 493 / Nine Mile phase I.

    Entry informationi

    Entry nameiMETK_COXBU
    AccessioniPrimary (citable) accession number: Q83A78
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 25, 2003
    Last sequence update: June 1, 2003
    Last modified: October 1, 2014
    This is version 80 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Coxiella burnetii
      Coxiella burnetii (strain RSA 493): entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3