Q83A36 (AROD_COXBU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-dehydroquinate dehydratase
Short name=3-dehydroquinase
EC=4.2.1.10

Alternative name(s):
Type I DHQase

Gene names

Name:	aroD
Ordered Locus Names:	CBU_2075

Organism

Coxiella burnetii (strain RSA 493 / Nine Mile phase I) [Reference proteome] [HAMAP]

Taxonomic identifier

227377 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Legionellales › Coxiellaceae › Coxiella › Coxiella burnetii

Protein attributes

Sequence length	233 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	3-dehydroquinate = 3-dehydroshikimate + H2O. HAMAP-Rule MF_00214
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 3/7. HAMAP-Rule MF_00214
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the type-I 3-dehydroquinase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Ligand	Schiff base
Molecular function	Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: HAMAP chorismate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	3-dehydroquinate dehydratase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 233	233	3-dehydroquinate dehydratase HAMAP-Rule MF_00214		PRO_0000325521
Regions
	Region	34 – 36	3	Substrate binding By similarity
Sites
	Active site	118	1	Proton donor/acceptor By similarity
	Active site	145	1	Schiff-base intermediate with substrate By similarity
	Binding site	64	1	Substrate By similarity
	Binding site	185	1	Substrate By similarity
	Binding site	205	1	Substrate By similarity
	Binding site	209	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q83A36 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: C80AF9E997D8B2EC	FASTA	233	26,508
	10 20 30 40 50 60 MLNTPRICVV VIGKTLEEFL SQLEAAQTAV DFVELRIDYL EQINPNWVRI IKNHTHKKAI 70 80 90 100 110 120 LCCRARADGG KFLGTPEAQQ EILQAGNDLG FDYLDIDLPV ANKISIHEKK AKIIISYHNF 130 140 150 160 170 180 LHTPPITELN FLLENMRLFN PDVFKFATKS EQYEDVKTLF KLLINKKNNE NMIVLGMGEQ 190 200 210 220 230 GKIIRLLSPL LGGYLTFSSI NGAISAPGQI DFKTMQDFYQ RFYKISSPLK GED

References

[1]

"Complete genome sequence of the Q-fever pathogen, Coxiella burnetii." Seshadri R., Paulsen I.T., Eisen J.A., Read T.D., Nelson K.E., Nelson W.C., Ward N.L., Tettelin H., Davidsen T.M., Beanan M.J., DeBoy R.T., Daugherty S.C., Brinkac L.M., Madupu R., Dodson R.J., Khouri H.M., Lee K.H., Carty H.A. , Scanlan D., Heinzen R.A., Thompson H.A., Samuel J.E., Fraser C.M., Heidelberg J.F. Proc. Natl. Acad. Sci. U.S.A. 100:5455-5460(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: RSA 493 / Nine Mile phase I.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016828 Genomic DNA. Translation: AAO91559.1.
RefSeq	NP_821045.1. NC_002971.3.
3D structure databases
HSSP	HSSP built from PDB template 2GPT based on UniProtKB Q9SQT8.
ProteinModelPortal	Q83A36.
ModBase	Search...
Protein-protein interaction databases
STRING	227377.CBU_2075.
Proteomic databases
PRIDE	Q83A36.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAO91559; AAO91559; CBU_2075.
GeneID	1209988.
KEGG	cbu:CBU_2075.
PATRIC	17932897. VBICoxBur82552_2065.
Phylogenomic databases
eggNOG	COG0710.
HOGENOM	HOG000033727.
KO	K03785.
OMA	IFTVRRR.
ProtClustDB	CLSK915225.
Enzyme and pathway databases
BioCyc	CBUR227377:GJ7S-2041-MONOMER.
UniPathway	UPA00053; UER00086.
Family and domain databases
Gene3D	3.20.20.70. 1 hit.
HAMAP	MF_00214. AroD.
InterPro	IPR013785. Aldolase_TIM. IPR001381. DHquinase_I. [Graphical view]
Pfam	PF01487. DHquinase_I. 1 hit. [Graphical view]
TIGRFAMs	TIGR01093. aroD. 1 hit.
PROSITE	PS01028. DEHYDROQUINASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AROD_COXBU

Accession

Primary (citable) accession number: Q83A36

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 18, 2008
Last sequence update:	June 1, 2003
Last modified:	May 1, 2013
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program