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Q839Z1 (Q839Z1_ENTFA) Unreviewed, UniProtKB/TrEMBL

Last modified April 16, 2014. Version 94. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Protein attributes

Sequence length642 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings By similarity. HAMAP-Rule MF_01898

Catalytic activity

ATP-dependent breakage, passage and rejoining of double-stranded DNA. HAMAP-Rule MF_01898 SAAS SAAS013760

Cofactor

Magnesium. Binds two Mg2+ per subunit. The magnesium ions form salt bridges with both the protein and the DNA. Can also accept other divalent metal cations, such as Mn2+ and Ca2+ By similarity. HAMAP-Rule MF_01898

Subunit structure

Heterotetramer, composed of two GyrA and two GyrB chains. Within the heterotetramer, GyrA contains the active site tyrosine that forms a covalent intermediate with the DNA, while GyrB contributes the cofactor binding sites and catalyzes ATP hydrolysis By similarity. HAMAP-Rule MF_01898

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_01898.

Sequence similarities

Belongs to the type II topoisomerase family. RuleBase RU000380 HAMAP-Rule MF_01898

Contains 1 Toprim domain. HAMAP-Rule MF_01898

Contains Toprim domain. SAAS SAAS002288

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain422 – 536115Toprim By similarity HAMAP-Rule MF_01898

Sites

Metal binding4281Magnesium 1; catalytic By similarity HAMAP-Rule MF_01898
Metal binding5011Magnesium 1; catalytic By similarity HAMAP-Rule MF_01898
Metal binding5011Magnesium 2 By similarity HAMAP-Rule MF_01898
Metal binding5031Magnesium 2 By similarity HAMAP-Rule MF_01898
Site4531Interaction with DNA By similarity HAMAP-Rule MF_01898
Site4561Interaction with DNA By similarity HAMAP-Rule MF_01898

Sequences

Sequence LengthMass (Da)Tools
Q839Z1 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: 7A5FD53203C6AEE0

FASTA64271,878
        10         20         30         40         50         60 
MRERAQEYDA SQIQVLEGLE AVRKRPGMYI GSTSGEGLHH LVWEIVDNSI DEALAGFAKS 

        70         80         90        100        110        120 
IQVIIEPDDS ITVIDDGRGI PVGIQAKTGR PAVETVFTVL HAGGKFGGGG YKVSGGLHGV 

       130        140        150        160        170        180 
GSSVVNALST SLDVRVYKDG KVYYQEYRRG AVVDDLKVIE ETDRHGTTVH FIPDPEIFTE 

       190        200        210        220        230        240 
TTVYDFDKLA TRVRELAFLN RGLHISIEDR REGQEDKKEY HYEGGIKSYV EHLNANKDVI 

       250        260        270        280        290        300 
FPEPIFIEGE QQDITVEVSM QYTDGYHSNI LSFANNIHTY EGGTHESGFK TSLTRVINDY 

       310        320        330        340        350        360 
ARKQKLMKEN DEKLTGEDVR EGLTAVVSIK HPDPQFEGQT KTKLGNSEVR TVTDRLFSEY 

       370        380        390        400        410        420 
FTKFLMENPT VGKQIVEKGM LASKARLAAK RAREVTRRKG ALEISNLPGK LADCSSKDPE 

       430        440        450        460        470        480 
KCELFIVEGD SAGGSAKQGR SREFQAILPI RGKILNVEKA SMDKILANEE IRSLFTAMGT 

       490        500        510        520        530        540 
GFGEDFDVSK ARYHKLVIMT DADVDGAHIR TLLLTLFYRF MRPIVEAGYV YIAQPPLYGV 

       550        560        570        580        590        600 
KQGKNITYVQ PGKHAEEELA KVLEELPASP KPSVQRYKGL GEMDDHQLWE TTMDPEKRLM 

       610        620        630        640 
ARVSVDDAIE ADQIFEMLMG DRVEPRRAFI EENAHYVKNL DI 

« Hide

References

« Hide 'large scale' references
[1]"Role of mobile DNA in the evolution of vancomycin-resistant Enterococcus faecalis."
Paulsen I.T., Banerjei L., Myers G.S.A., Nelson K.E., Seshadri R., Read T.D., Fouts D.E., Eisen J.A., Gill S.R., Heidelberg J.F., Tettelin H., Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J., Daugherty S.C., DeBoy R.T., Durkin S.A. expand/collapse author list , Kolonay J.F., Madupu R., Nelson W.C., Vamathevan J.J., Tran B., Upton J., Hansen T., Shetty J., Khouri H.M., Utterback T.R., Radune D., Ketchum K.A., Dougherty B.A., Fraser C.M.
Science 299:2071-2074(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 700802 / V583.
[2]"Pyrrolopyrimidine inhibitors of DNA gyrase B (GyrB) and topoisomerase IV (ParE). Part I: Structure guided discovery and optimization of dual targeting agents with potent, broad-spectrum enzymatic activity."
Tari L.W., Trzoss M., Bensen D.C., Li X., Chen Z., Lam T., Zhang J., Creighton C.J., Cunningham M.L., Kwan B., Stidham M., Shaw K.J., Lightstone F.C., Wong S.E., Nguyen T.B., Nix J., Finn J.
Bioorg. Med. Chem. Lett. 23:1529-1536(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.69 ANGSTROMS) OF 18-224.
[3]"A new class of type IIA topoisomerase inhibitors with broad-spectrum antibacterial activity."
Tari L.W., Bensen D.C., Finn J.
Submitted (APR-2013) to the PDB data bank
Cited for: X-RAY CRYSTALLOGRAPHY (1.30 ANGSTROMS) OF 18-224.
[4]"A new class of type iia topoisomerase inhibitors with broad-spectrum antibacterial activity."
Tari L.W., Bensen D.C., Finn J.
Submitted (MAY-2013) to the PDB data bank
Cited for: X-RAY CRYSTALLOGRAPHY (1.70 ANGSTROMS) OF 18-224.
[5]"A new class of type iia topoisomerase inhibitors withbroad-spectrum antibacterial activity."
Tari L.W., Bensen D.C., Finn J.
Submitted (MAY-2013) to the PDB data bank
Cited for: X-RAY CRYSTALLOGRAPHY (1.75 ANGSTROMS) OF 18-224.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE016830 Genomic DNA. Translation: AAO79890.1.
RefSeqNP_813818.1. NC_004668.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
4GEEX-ray1.70A18-224[»]
4GFNX-ray1.90A18-224[»]
4GGLX-ray1.69A18-224[»]
4HXWX-ray1.69A18-224[»]
4K4OX-ray1.30A18-224[»]
4KSGX-ray1.75A18-224[»]
4KSHX-ray1.70A18-224[»]
4KTNX-ray1.69A18-224[»]
ProteinModelPortalQ839Z1.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING226185.EF0005.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAO79890; AAO79890; EF_0005.
GeneID1198916.
KEGGefa:EF0005.
PATRIC21850447. VBIEntFae7065_0005.

Phylogenomic databases

KOK02470.
OMANINTREG.
OrthoDBEOG6P334W.
ProtClustDBPRK05644.

Enzyme and pathway databases

BioCycEFAE226185:GHI1-5-MONOMER.

Family and domain databases

Gene3D3.30.230.10. 1 hit.
3.30.565.10. 1 hit.
3.40.50.670. 1 hit.
HAMAPMF_01898. GyrB.
InterProIPR002288. DNA_gyrase_B_C.
IPR011557. GyrB.
IPR003594. HATPase_ATP-bd.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
IPR001241. Topo_IIA.
IPR013506. Topo_IIA_bsu_dom2.
IPR013759. Topo_IIA_cen_dom.
IPR013760. Topo_IIA_like_dom.
IPR018522. TopoIIA_CS.
IPR006171. Toprim_domain.
[Graphical view]
PfamPF00204. DNA_gyraseB. 1 hit.
PF00986. DNA_gyraseB_C. 1 hit.
PF02518. HATPase_c. 1 hit.
PF01751. Toprim. 1 hit.
[Graphical view]
PRINTSPR00418. TPI2FAMILY.
SMARTSM00387. HATPase_c. 1 hit.
SM00433. TOP2c. 1 hit.
[Graphical view]
SUPFAMSSF54211. SSF54211. 1 hit.
SSF55874. SSF55874. 1 hit.
SSF56719. SSF56719. 1 hit.
TIGRFAMsTIGR01059. gyrB. 1 hit.
PROSITEPS00177. TOPOISOMERASE_II. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameQ839Z1_ENTFA
AccessionPrimary (citable) accession number: Q839Z1
Entry history
Integrated into UniProtKB/TrEMBL: June 1, 2003
Last sequence update: June 1, 2003
Last modified: April 16, 2014
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)