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Protein

DNA gyrase subunit B

Gene

gyrB

Organism
Enterococcus faecalis (strain ATCC 700802 / V583)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings.UniRule annotation2 Publications

Catalytic activityi

ATP-dependent breakage, passage and rejoining of double-stranded DNA.UniRule annotation

Cofactori

Mg2+UniRule annotation, Mn2+UniRule annotation, Ca2+UniRule annotationNote: Binds two Mg2+ per subunit. The magnesium ions form salt bridges with both the protein and the DNA. Can also accept other divalent metal cations, such as Mn2+ or Ca2+.UniRule annotation

Enzyme regulationi

Pyrrolopyrimidines inhibit both GyrB and its paralog in topoisomerase IV (parE) (PubMed:23352267).2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi428Magnesium 1; catalyticUniRule annotation1
Metal bindingi501Magnesium 1; catalyticUniRule annotation1
Metal bindingi501Magnesium 2UniRule annotation1
Metal bindingi503Magnesium 2UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Isomerase, Topoisomerase
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEFAE226185:G1G0C-5-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
DNA gyrase subunit BUniRule annotation (EC:5.99.1.3UniRule annotation)
Gene namesi
Name:gyrBUniRule annotation
Ordered Locus Names:EF_0005
OrganismiEnterococcus faecalis (strain ATCC 700802 / V583)
Taxonomic identifieri226185 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesEnterococcaceaeEnterococcus
Proteomesi
  • UP000001415 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004355401 – 642DNA gyrase subunit BAdd BLAST642

Interactioni

Subunit structurei

Heterotetramer, composed of two GyrA and two GyrB chains. Within the heterotetramer, GyrA contains the active site tyrosine that forms a covalent intermediate with the DNA, while GyrB contributes the cofactor binding sites and catalyzes ATP hydrolysis.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei453Interaction with DNAUniRule annotation1
Sitei456Interaction with DNAUniRule annotation1

Protein-protein interaction databases

STRINGi226185.EF0005

Structurei

Secondary structure

1642
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi19 – 24Combined sources6
Helixi26 – 30Combined sources5
Helixi35 – 54Combined sources20
Beta strandi60 – 65Combined sources6
Beta strandi71 – 75Combined sources5
Turni86 – 88Combined sources3
Beta strandi89 – 91Combined sources3
Helixi92 – 98Combined sources7
Helixi122 – 127Combined sources6
Beta strandi129 – 138Combined sources10
Beta strandi141 – 148Combined sources8
Beta strandi157 – 161Combined sources5
Beta strandi166 – 173Combined sources8
Turni175 – 177Combined sources3
Helixi186 – 199Combined sources14
Turni200 – 202Combined sources3
Beta strandi204 – 209Combined sources6
Beta strandi216 – 221Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4GEEX-ray1.70A18-224[»]
4GFNX-ray1.90A18-224[»]
4GGLX-ray1.69A18-224[»]
4HXWX-ray1.69A18-224[»]
4K4OX-ray1.30A18-224[»]
4KSGX-ray1.75A18-224[»]
4KSHX-ray1.70A18-224[»]
4KTNX-ray1.69A18-224[»]
ProteinModelPortaliQ839Z1
SMRiQ839Z1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini422 – 536ToprimUniRule annotationAdd BLAST115

Sequence similaritiesi

Belongs to the type II topoisomerase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C7D Bacteria
COG0187 LUCA
KOiK02470
OMAiDCSSRDP

Family and domain databases

CDDicd00075 HATPase_c, 1 hit
cd03366 TOPRIM_TopoIIA_GyrB, 1 hit
Gene3Di3.30.230.101 hit
3.30.565.101 hit
3.40.50.6701 hit
HAMAPiMF_01898 GyrB, 1 hit
InterProiView protein in InterPro
IPR002288 DNA_gyrase_B_C
IPR011557 GyrB
IPR003594 HATPase_C
IPR036890 HATPase_C_sf
IPR020568 Ribosomal_S5_D2-typ_fold
IPR014721 Ribosomal_S5_D2-typ_fold_subgr
IPR001241 Topo_IIA
IPR013760 Topo_IIA-like_dom_sf
IPR013759 Topo_IIA_B_C
IPR013506 Topo_IIA_bsu_dom2
IPR018522 TopoIIA_CS
IPR006171 TOPRIM_domain
IPR034160 TOPRIM_GyrB
PANTHERiPTHR10169 PTHR10169, 1 hit
PfamiView protein in Pfam
PF00204 DNA_gyraseB, 1 hit
PF00986 DNA_gyraseB_C, 1 hit
PF02518 HATPase_c, 1 hit
PF01751 Toprim, 1 hit
SMARTiView protein in SMART
SM00387 HATPase_c, 1 hit
SM00433 TOP2c, 1 hit
SUPFAMiSSF54211 SSF54211, 1 hit
SSF55874 SSF55874, 1 hit
SSF56719 SSF56719, 1 hit
TIGRFAMsiTIGR01059 gyrB, 1 hit
PROSITEiView protein in PROSITE
PS00177 TOPOISOMERASE_II, 1 hit
PS50880 TOPRIM, 1 hit

Sequencei

Sequence statusi: Complete.

Q839Z1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRERAQEYDA SQIQVLEGLE AVRKRPGMYI GSTSGEGLHH LVWEIVDNSI
60 70 80 90 100
DEALAGFAKS IQVIIEPDDS ITVIDDGRGI PVGIQAKTGR PAVETVFTVL
110 120 130 140 150
HAGGKFGGGG YKVSGGLHGV GSSVVNALST SLDVRVYKDG KVYYQEYRRG
160 170 180 190 200
AVVDDLKVIE ETDRHGTTVH FIPDPEIFTE TTVYDFDKLA TRVRELAFLN
210 220 230 240 250
RGLHISIEDR REGQEDKKEY HYEGGIKSYV EHLNANKDVI FPEPIFIEGE
260 270 280 290 300
QQDITVEVSM QYTDGYHSNI LSFANNIHTY EGGTHESGFK TSLTRVINDY
310 320 330 340 350
ARKQKLMKEN DEKLTGEDVR EGLTAVVSIK HPDPQFEGQT KTKLGNSEVR
360 370 380 390 400
TVTDRLFSEY FTKFLMENPT VGKQIVEKGM LASKARLAAK RAREVTRRKG
410 420 430 440 450
ALEISNLPGK LADCSSKDPE KCELFIVEGD SAGGSAKQGR SREFQAILPI
460 470 480 490 500
RGKILNVEKA SMDKILANEE IRSLFTAMGT GFGEDFDVSK ARYHKLVIMT
510 520 530 540 550
DADVDGAHIR TLLLTLFYRF MRPIVEAGYV YIAQPPLYGV KQGKNITYVQ
560 570 580 590 600
PGKHAEEELA KVLEELPASP KPSVQRYKGL GEMDDHQLWE TTMDPEKRLM
610 620 630 640
ARVSVDDAIE ADQIFEMLMG DRVEPRRAFI EENAHYVKNL DI
Length:642
Mass (Da):71,878
Last modified:June 1, 2003 - v1
Checksum:i7A5FD53203C6AEE0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016830 Genomic DNA Translation: AAO79890.1
RefSeqiNP_813818.1, NC_004668.1
WP_002379278.1, NC_004668.1

Genome annotation databases

EnsemblBacteriaiAAO79890; AAO79890; EF_0005
GeneIDi1198916
KEGGiefa:EF0005
PATRICifig|226185.9.peg.5

Similar proteinsi

Entry informationi

Entry nameiGYRB_ENTFA
AccessioniPrimary (citable) accession number: Q839Z1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 17, 2016
Last sequence update: June 1, 2003
Last modified: February 28, 2018
This is version 120 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome