Reviewed, UniProtKB/Swiss-Prot Q839Q5 (OTCC_ENTFA)

Names and origin

Protein names

Recommended name:
    Ornithine carbamoyltransferase, catabolic
      Short name=OTCase
    EC=2.1.3.3

Gene names

Name:	arcB
Ordered Locus Names:	EF_0105

Organism

Enterococcus faecalis (Streptococcus faecalis) [Complete proteome] [HAMAP]

Taxonomic identifier

1351 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Enterococcaceae › Enterococcus

Protein attributes

Sequence length	339 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Carbamoyl phosphate + L-ornithine = phosphate + L-citrulline. HAMAP MF_01109
Pathway	Amino-acid degradation; L-arginine degradation via ADI pathway; carbamoyl phosphate from L-arginine: step 2/2. HAMAP MF_01109
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the ATCase/OTCase family.

Ontologies

Keywords
Biological process	Arginine metabolism
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	ornithine carbamoyltransferase complex Inferred from electronic annotation. Source: InterPro
Molecular function	amino acid binding Inferred from electronic annotation. Source: InterPro ornithine carbamoyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 339	339	Ornithine carbamoyltransferase, catabolic HAMAP MF_01109		PRO_0000112924
Regions
	Region	57 – 61	5	Carbamoyl phosphate binding By similarity
	Region	273 – 276	4	Ornithine binding By similarity
Sites
	Binding site	108	1	Carbamoyl phosphate By similarity
	Binding site	135	1	Carbamoyl phosphate By similarity
	Site	32	1	Important for structural integrity By similarity
	Site	148	1	Important for structural integrity By similarity
Experimental info
	Sequence conflict	17	1	S → T in CAC41342. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q839Q5-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 2238D2AA1D4A075C	FASTA	339	38,029
	10 20 30 40 50 60 MNSVFQGRSL LAEKDFSRAE LEYLVDFSIH LKELKKKGIP HHYLEGKNIA LLFEKTSTRT 70 80 90 100 110 120 RSAFTTAAID LGAHPEYLGA NDIQLGKKES VEDTAIVLGS MFDGIEFRGF SQEVVEDLAK 130 140 150 160 170 180 YSGVPVWNGL TDQWHPTQMI ADFMTVKENF GRLEGITLVY VGDGRNNMAN SLLVTGAILG 190 200 210 220 230 240 VNVRICAPKE LFPSDEVVNY AKEFAKESGA ELMITDDVAK GVKGANVLYT DVWVSMGEED 250 260 270 280 290 300 KFEERVNLLK PYQINMAMLE KTENMDGDLI VLHCLPAFHD TKTQYGEMVA EKFGITEMEI 310 320 330 TDEVFRSKYG RQFEEAENRM HSIKAIMAAT LGNLFIPRV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Carbamate kinase from Enterococcus faecalis and Enterococcus faecium: cloning of the genes, studies on the enzyme expressed in Escherichia coli, and sequence similarity with N-acetyl-L-glutamate kinase." Marina A., Uriarte M., Barcelona B., Fresquet V., Cervera J., Rubio V. Eur. J. Biochem. 253:280-291(1998) [PubMed: 9578487] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 29212 / DSM 2570.
[2]	"Role of mobile DNA in the evolution of vancomycin-resistant Enterococcus faecalis." Paulsen I.T., Banerjei L., Myers G.S.A., Nelson K.E., Seshadri R., Read T.D., Fouts D.E., Eisen J.A., Gill S.R., Heidelberg J.F., Tettelin H., Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J., Daugherty S.C., DeBoy R.T., Durkin S.A. , Kolonay J.F., Madupu R., Nelson W.C., Vamathevan J.J., Tran B., Upton J., Hansen T., Shetty J., Khouri H.M., Utterback T.R., Radune D., Ketchum K.A., Dougherty B.A., Fraser C.M. Science 299:2071-2074(2003) [PubMed: 12663927] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: V583 / ATCC 700802.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ312276 Genomic DNA. Translation: CAC41342.1. AE016830 Genomic DNA. Translation: AAO79980.1.
RefSeq	NP_813908.1.
3D structure databases
HSSP	HSSP built from PDB template 1DXH based on UniProtKB P08308.
ModBase	Search...
Genome annotation databases
GeneID	1199006.
GenomeReviews	Gene locus EF_0105 in contig AE016830_GR.
KEGG	efa:EF0105.
NMPDR	fig|226185.1.peg.95.
TIGR	EF_0105.
Phylogenomic databases
HOGENOM	Q839Q5.
OMA	FRSKHSK.
Enzyme and pathway databases
BioCyc	EFAE226185:EF_0105-MON.
BRENDA	2.1.3.3. 704.
Family and domain databases
HAMAP	MF_01109. [Tree]
InterPro	IPR006132. Asp/Orn_carbamoyltranf_P_bd. IPR006130. Asp/Orn_carbamoylTrfase. IPR006131. Asp_carbamoyltransf_Asp/Orn_bd. IPR002292. Orn_carbamltrans. [Graphical view]
Pfam	PF00185. OTCace. 1 hit. PF02729. OTCace_N. 1 hit. [Graphical view]
PRINTS	PR00100. AOTCASE. PR00102. OTCASE.
TIGRFAMs	TIGR00658. orni_carb_tr. 1 hit.
PROSITE	PS00097. CARBAMOYLTRANSFERASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

OTCC_ENTFA

Accession

Primary (citable) accession number: Q839Q5
Secondary accession number(s): Q93K66

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 11, 2003
Last sequence update:	June 1, 2003
Last modified:	November 3, 2009
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)