Q839J4 (ADD_ENTFA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Adenosine deaminase
EC=3.5.4.4

Alternative name(s):
Adenosine aminohydrolase

Gene names

Name:	add
Ordered Locus Names:	EF_0171

Organism

Enterococcus faecalis (Streptococcus faecalis)

Taxonomic identifier

1351 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Enterococcaceae › Enterococcus

Protein attributes

Sequence length	337 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Adenosine + H2O = inosine + NH3. HAMAP MF_00540
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00540
Sequence similarities	Belongs to the adenosine and AMP deaminases family. Adenosine deaminase subfamily.

Ontologies

Keywords
Biological process	Nucleotide metabolism
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	purine ribonucleoside monophosphate biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	adenosine deaminase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 337	337	Adenosine deaminase HAMAP MF_00540		PRO_0000194370
Sites
	Active site	202	1	Proton donor By similarity
	Metal binding	15	1	Zinc; catalytic By similarity
	Metal binding	17	1	Zinc; catalytic By similarity
	Metal binding	199	1	Zinc; catalytic By similarity
	Metal binding	279	1	Zinc; catalytic By similarity
	Binding site	17	1	Substrate By similarity
	Binding site	19	1	Substrate By similarity
	Binding site	172	1	Substrate; via amide nitrogen and carbonyl oxygen By similarity
	Site	222	1	Important for catalytic activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q839J4 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: DA72368463DA25F3	FASTA	337	37,362
	10 20 30 40 50 60 MEESRVRQLP KIELHCHLDG SIRPTTLRTI AEKQNIPLPQ DEQALKELVV APEKCTDLND 70 80 90 100 110 120 YLTRFDFVLT CLQTAEALQA AAYDVISQAA EDGVAYIEVR FAPSQHTEKG LRLPEIVTAV 130 140 150 160 170 180 LTGLKQGEED FGVKSNALLC GMRHDQQQAI EKIVHLAHDF RETGVVGFDL AGNEVDFPPY 190 200 210 220 230 240 TFEDVLALAN QLSIPLTLHA GECGCGKNVA DAVTLGATRI GHGIALKDTP EYLALLKEKK 250 260 270 280 290 300 VLLEMCPTSN FQTGTVKTLA EYPFQQFIEA GLAVCINTDN RTVSDTTLTK EFMKLATWYQ 310 320 330 LSYDEMKQLT KNALAGAFLS PDEKKLLNQK IDQAYLF

References

[1]

"Role of mobile DNA in the evolution of vancomycin-resistant Enterococcus faecalis." Paulsen I.T., Banerjei L., Myers G.S.A., Nelson K.E., Seshadri R., Read T.D., Fouts D.E., Eisen J.A., Gill S.R., Heidelberg J.F., Tettelin H., Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J., Daugherty S.C., DeBoy R.T., Durkin S.A. , Kolonay J.F., Madupu R., Nelson W.C., Vamathevan J.J., Tran B., Upton J., Hansen T., Shetty J., Khouri H.M., Utterback T.R., Radune D., Ketchum K.A., Dougherty B.A., Fraser C.M. Science 299:2071-2074(2003) [PubMed: 12663927] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700802 / V583.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016830 Genomic DNA. Translation: AAO80045.1.
RefSeq	NP_813973.1. NC_004668.1.
3D structure databases
ProteinModelPortal	Q839J4.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1199071.
GenomeReviews	Gene locus EF_0171 in contig AE016830_GR.
KEGG	efa:EF0171.
NMPDR	fig|226185.1.peg.160.
PATRIC	21850753. VBIEntFae7065_0158.
TIGR	EF_0171.
Phylogenomic databases
HOGENOM	HBG630382.
OMA	PYYMAMN.
ProtClustDB	PRK09358.
Enzyme and pathway databases
BioCyc	EFAE226185:EF_0171-MONOMER.
Family and domain databases
HAMAP	MF_00540. A_deaminase. [Tree]
InterPro	IPR001365. A/AMP_deaminase_dom. IPR006330. A_deaminase. [Graphical view]
KO	K01488.
PANTHER	PTHR11409:SF21. PTHR11409:SF21. 1 hit.
Pfam	PF00962. A_deaminase. 1 hit. [Graphical view]
TIGRFAMs	TIGR01430. Aden_deam. 1 hit.
PROSITE	PS00485. A_DEAMINASE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ADD_ENTFA

Accession

Primary (citable) accession number: Q839J4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 2, 2004
Last sequence update:	June 1, 2003
Last modified:	January 25, 2012
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program