Reviewed, UniProtKB/Swiss-Prot Q839C1 (LDH1_ENTFA)

Names and origin

Protein names

Recommended name:
    L-lactate dehydrogenase 1
      Short name=L-LDH 1
    EC=1.1.1.27

Gene names

Name:	ldh1
Synonyms:	ldh-1
Ordered Locus Names:	EF_0255

Organism

Enterococcus faecalis (Streptococcus faecalis) [Complete proteome] [HAMAP]

Taxonomic identifier

1351 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Enterococcaceae › Enterococcus

Protein attributes

Sequence length	327 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-lactate + NAD(+) = pyruvate + NADH.
Pathway	Fermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1.
Subunit structure	Homotetramer By similarity.
Subcellular location	CytoplasmBy similarity.
Sequence similarities	Belongs to the LDH/MDH superfamily. LDH family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Oxidoreductase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	anaerobic glycolysis Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	L-lactate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 327	327	L-lactate dehydrogenase 1		PRO_0000168343
Regions
	Nucleotide binding	19 – 47	29	NAD By similarity
Sites
	Active site	182	1	Proton acceptor By similarity
	Binding site	95	1	Substrate By similarity
	Binding site	127	1	NAD or substrate By similarity
	Binding site	158	1	Substrate By similarity
	Binding site	236	1	Substrate By similarity
Amino acid modifications
	Modified residue	227	1	Phosphotyrosine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q839C1-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: E7D6F6D13D0C07AB	FASTA	327	35,487
	10 20 30 40 50 60 MTAAAGNKDH QKVILVGDGA VGSSYAFALV TQNIAQEVGI IDINVPKTEG DALDLSHALA 70 80 90 100 110 120 FTSPKKIYAA TYDDCHDADL VVLTAGAPQK PGETRLDLVH KNLKINKEIV TTIVDSGFNG 130 140 150 160 170 180 IFLVAANPVD ILTYSTWKFS GFPKERVIGS GTSLDSARFR QAIAELVDVD ARNVHAYILG 190 200 210 220 230 240 EHGDTEFPVW SHANVAGLQI YEWVKNNPDV DEEAMVNLFF NVRDAAYTII EKKGATFYGI 250 260 270 280 290 300 AVALARITKA ILNDENSVLP LSVYLEGEYG QNDIYIGAPA IINRQGVKQV IEIPLTDAEQ 310 320 EKMEASASAL KEVIETAFAK FEAEEAK

References

[1]

"Role of mobile DNA in the evolution of vancomycin-resistant Enterococcus faecalis." Paulsen I.T., Banerjei L., Myers G.S.A., Nelson K.E., Seshadri R., Read T.D., Fouts D.E., Eisen J.A., Gill S.R., Heidelberg J.F., Tettelin H., Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J., Daugherty S.C., DeBoy R.T., Durkin S.A. , Kolonay J.F., Madupu R., Nelson W.C., Vamathevan J.J., Tran B., Upton J., Hansen T., Shetty J., Khouri H.M., Utterback T.R., Radune D., Ketchum K.A., Dougherty B.A., Fraser C.M. Science 299:2071-2074(2003) [PubMed: 12663927] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: V583 / ATCC 700802.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE016830 Genomic DNA. Translation: AAO80120.1.
RefSeq	NP_814049.1.
3D structure databases
HSSP	HSSP built from PDB template 2LDB based on UniProtKB P00344.
SMR	Q839C1. Positions 3-320.
ModBase	Search...
Genome annotation databases
GeneID	1199146.
GenomeReviews	Gene locus EF_0255 in contig AE016830_GR.
KEGG	efa:EF0255.
NMPDR	fig|226185.1.peg.235.
TIGR	EF_0255.
Phylogenomic databases
HOGENOM	Q839C1.
Enzyme and pathway databases
BioCyc	EFAE226185:EF_0255-MON.
Family and domain databases
HAMAP	MF_00488. [Tree]
InterPro	IPR001557. L-lactate/malate_DHase. IPR011304. L-lactate_DHase. IPR001236. Lactate/malate_DHase. IPR015955. Lactate_DHase/Glyco_Ohase_4_C. IPR016040. NAD(P)-bd. [Graphical view]
Gene3D	G3DSA:3.90.110.10. lact_mal_DH. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF02866. Ldh_1_C. 1 hit. PF00056. Ldh_1_N. 1 hit. [Graphical view]
PIRSF	PIRSF000102. Lac_mal_DH. 1 hit.
PRINTS	PR00086. LLDHDRGNASE.
TIGRFAMs	TIGR01771. L-LDH-NAD. 1 hit.
PROSITE	PS00064. L_LDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LDH1_ENTFA

Accession

Primary (citable) accession number: Q839C1

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 21, 2004
Last sequence update:	June 1, 2003
Last modified:	November 25, 2008
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)