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Q838I3

- DAPF_ENTFA

UniProt

Q838I3 - DAPF_ENTFA

Protein

Diaminopimelate epimerase

Gene

dapF

Organism
Enterococcus faecalis (strain ATCC 700802 / V583)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 67 (01 Oct 2014)
      Sequence version 1 (01 Jun 2003)
      Previous versions | rss
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    Functioni

    Catalyzes the stereoinversion of LL-2,6-diaminoheptanedioate (L,L-DAP) to meso-diaminoheptanedioate (meso-DAP), a precursor of L-lysine and an essential component of the bacterial peptidoglycan.UniRule annotation

    Catalytic activityi

    LL-2,6-diaminoheptanedioate = meso-diaminoheptanedioate.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei13 – 131SubstrateUniRule annotation
    Binding sitei52 – 521SubstrateUniRule annotation
    Binding sitei72 – 721SubstrateUniRule annotation
    Active sitei81 – 811Proton donor/acceptorUniRule annotation
    Binding sitei169 – 1691SubstrateUniRule annotation
    Sitei171 – 1711Important for catalytic activityUniRule annotation
    Binding sitei205 – 2051SubstrateUniRule annotation
    Sitei223 – 2231Important for catalytic activityUniRule annotation
    Active sitei232 – 2321Proton donor/acceptorUniRule annotation

    GO - Molecular functioni

    1. diaminopimelate epimerase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. lysine biosynthetic process via diaminopimelate Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Isomerase

    Keywords - Biological processi

    Amino-acid biosynthesis, Lysine biosynthesis

    Enzyme and pathway databases

    BioCyciEFAE226185:GHI1-467-MONOMER.
    UniPathwayiUPA00034; UER00025.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Diaminopimelate epimeraseUniRule annotation (EC:5.1.1.7UniRule annotation)
    Short name:
    DAP epimeraseUniRule annotation
    Gene namesi
    Name:dapFUniRule annotation
    Ordered Locus Names:EF_0464
    OrganismiEnterococcus faecalis (strain ATCC 700802 / V583)
    Taxonomic identifieri226185 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesEnterococcaceaeEnterococcus
    ProteomesiUP000001415: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 326326Diaminopimelate epimerasePRO_1000011876Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi81 ↔ 232

    Keywords - PTMi

    Disulfide bond

    Interactioni

    Protein-protein interaction databases

    STRINGi226185.EF0464.

    Structurei

    3D structure databases

    ProteinModelPortaliQ838I3.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni81 – 833Substrate bindingUniRule annotation
    Regioni223 – 2242Substrate bindingUniRule annotation
    Regioni233 – 2342Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the diaminopimelate epimerase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0253.
    KOiK01778.
    OMAiKFNDITT.
    OrthoDBiEOG6ND0M5.

    Family and domain databases

    HAMAPiMF_00197. DAP_epimerase.
    InterProiIPR018510. DAP_epimerase_AS.
    IPR001653. DAP_epimerase_DapF.
    [Graphical view]
    PANTHERiPTHR31689. PTHR31689. 1 hit.
    PfamiPF01678. DAP_epimerase. 2 hits.
    [Graphical view]
    TIGRFAMsiTIGR00652. DapF. 1 hit.
    PROSITEiPS01326. DAP_EPIMERASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q838I3-1 [UniParc]FASTAAdd to Basket

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    MNVMMQKVHG SENDFFLLDE TQFERSLTAE EIEQLRIQLC SRETGLLAGA    50
    DGLLLVGEGT HGTSNARMRV INSDGSEASM CGNGLRTVAR YLAEKNQEKS 100
    FTVETMFADL KVRQAPNLAE EVATYQVEIS PVSFEAVTIP MHLGVQTLID 150
    EIVPALSNTI RFTAVAVPNP HLVAFVDHET LNGPEFERIA TYVNNENPYF 200
    PEGINVSFVE ILGKNQLFVR TYERGVGFTS ACGTAMCASS LLYTLLKDGV 250
    FYEEITVKNT GGMVKTVVHE TSDGSYWMEL IGNATITHLI EGSLTDLLNG 300
    AFEKITITET NEQKHYQEFL QTLSQK 326
    Length:326
    Mass (Da):36,095
    Last modified:June 1, 2003 - v1
    Checksum:iF89F02A0783A3BDA
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE016830 Genomic DNA. Translation: AAO80319.1.
    RefSeqiNP_814248.1. NC_004668.1.

    Genome annotation databases

    EnsemblBacteriaiAAO80319; AAO80319; EF_0464.
    GeneIDi1199379.
    KEGGiefa:EF0464.
    PATRICi21851353. VBIEntFae7065_0429.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE016830 Genomic DNA. Translation: AAO80319.1 .
    RefSeqi NP_814248.1. NC_004668.1.

    3D structure databases

    ProteinModelPortali Q838I3.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 226185.EF0464.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAO80319 ; AAO80319 ; EF_0464 .
    GeneIDi 1199379.
    KEGGi efa:EF0464.
    PATRICi 21851353. VBIEntFae7065_0429.

    Phylogenomic databases

    eggNOGi COG0253.
    KOi K01778.
    OMAi KFNDITT.
    OrthoDBi EOG6ND0M5.

    Enzyme and pathway databases

    UniPathwayi UPA00034 ; UER00025 .
    BioCyci EFAE226185:GHI1-467-MONOMER.

    Family and domain databases

    HAMAPi MF_00197. DAP_epimerase.
    InterProi IPR018510. DAP_epimerase_AS.
    IPR001653. DAP_epimerase_DapF.
    [Graphical view ]
    PANTHERi PTHR31689. PTHR31689. 1 hit.
    Pfami PF01678. DAP_epimerase. 2 hits.
    [Graphical view ]
    TIGRFAMsi TIGR00652. DapF. 1 hit.
    PROSITEi PS01326. DAP_EPIMERASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 700802 / V583.

    Entry informationi

    Entry nameiDAPF_ENTFA
    AccessioniPrimary (citable) accession number: Q838I3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: June 1, 2003
    Last modified: October 1, 2014
    This is version 67 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3