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Protein

Diaminopimelate epimerase

Gene

dapF

Organism
Enterococcus faecalis (strain ATCC 700802 / V583)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the stereoinversion of LL-2,6-diaminoheptanedioate (L,L-DAP) to meso-diaminoheptanedioate (meso-DAP), a precursor of L-lysine and an essential component of the bacterial peptidoglycan.UniRule annotation

Catalytic activityi

LL-2,6-diaminoheptanedioate = meso-diaminoheptanedioate.UniRule annotation

Pathwayi: L-lysine biosynthesis via DAP pathway

This protein is involved in step 1 of the subpathway that synthesizes DL-2,6-diaminopimelate from LL-2,6-diaminopimelate.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Diaminopimelate epimerase (dapF)
This subpathway is part of the pathway L-lysine biosynthesis via DAP pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes DL-2,6-diaminopimelate from LL-2,6-diaminopimelate, the pathway L-lysine biosynthesis via DAP pathway and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei13SubstrateUniRule annotation1
Binding sitei72SubstrateUniRule annotation1
Active sitei81Proton donorUniRule annotation1
Binding sitei169SubstrateUniRule annotation1
Sitei171Could be important to modulate the pK values of the two catalytic cysteine residuesUniRule annotation1
Binding sitei205SubstrateUniRule annotation1
Sitei223Could be important to modulate the pK values of the two catalytic cysteine residuesUniRule annotation1
Active sitei232Proton acceptorUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIsomerase
Biological processAmino-acid biosynthesis, Lysine biosynthesis

Enzyme and pathway databases

BioCyciEFAE226185:G1G0C-467-MONOMER
UniPathwayiUPA00034; UER00025

Names & Taxonomyi

Protein namesi
Recommended name:
Diaminopimelate epimeraseUniRule annotation (EC:5.1.1.7UniRule annotation)
Short name:
DAP epimeraseUniRule annotation
Alternative name(s):
PLP-independent amino acid racemaseUniRule annotation
Gene namesi
Name:dapFUniRule annotation
Ordered Locus Names:EF_0464
OrganismiEnterococcus faecalis (strain ATCC 700802 / V583)
Taxonomic identifieri226185 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesEnterococcaceaeEnterococcus
Proteomesi

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000118761 – 326Diaminopimelate epimeraseAdd BLAST326

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi226185.EF0464

Structurei

3D structure databases

ProteinModelPortaliQ838I3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni82 – 83Substrate bindingUniRule annotation2
Regioni223 – 224Substrate bindingUniRule annotation2
Regioni233 – 234Substrate bindingUniRule annotation2

Sequence similaritiesi

Belongs to the diaminopimelate epimerase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105E4Z Bacteria
COG0253 LUCA
KOiK01778
OMAiSMCGNGG

Family and domain databases

HAMAPiMF_00197 DAP_epimerase, 1 hit
InterProiView protein in InterPro
IPR018510 DAP_epimerase_AS
IPR001653 DAP_epimerase_DapF
PANTHERiPTHR31689 PTHR31689, 1 hit
PfamiView protein in Pfam
PF01678 DAP_epimerase, 2 hits
TIGRFAMsiTIGR00652 DapF, 1 hit
PROSITEiView protein in PROSITE
PS01326 DAP_EPIMERASE, 1 hit

Sequencei

Sequence statusi: Complete.

Q838I3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNVMMQKVHG SENDFFLLDE TQFERSLTAE EIEQLRIQLC SRETGLLAGA
60 70 80 90 100
DGLLLVGEGT HGTSNARMRV INSDGSEASM CGNGLRTVAR YLAEKNQEKS
110 120 130 140 150
FTVETMFADL KVRQAPNLAE EVATYQVEIS PVSFEAVTIP MHLGVQTLID
160 170 180 190 200
EIVPALSNTI RFTAVAVPNP HLVAFVDHET LNGPEFERIA TYVNNENPYF
210 220 230 240 250
PEGINVSFVE ILGKNQLFVR TYERGVGFTS ACGTAMCASS LLYTLLKDGV
260 270 280 290 300
FYEEITVKNT GGMVKTVVHE TSDGSYWMEL IGNATITHLI EGSLTDLLNG
310 320
AFEKITITET NEQKHYQEFL QTLSQK
Length:326
Mass (Da):36,095
Last modified:June 1, 2003 - v1
Checksum:iF89F02A0783A3BDA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016830 Genomic DNA Translation: AAO80319.1
RefSeqiNP_814248.1, NC_004668.1
WP_002387689.1, NZ_KE136524.1

Genome annotation databases

EnsemblBacteriaiAAO80319; AAO80319; EF_0464
GeneIDi1199379
KEGGiefa:EF0464
PATRICifig|226185.45.peg.2871

Similar proteinsi

Entry informationi

Entry nameiDAPF_ENTFA
AccessioniPrimary (citable) accession number: Q838I3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: June 1, 2003
Last modified: May 23, 2018
This is version 79 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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