Q838C9 (LDH2_ENTFA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
L-lactate dehydrogenase 2
Short name=L-LDH 2
EC=1.1.1.27

Gene names

Name:	ldh2
Synonyms:	ldh-2
Ordered Locus Names:	EF_0641

Organism

Enterococcus faecalis (Streptococcus faecalis)

Taxonomic identifier

1351 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Enterococcaceae › Enterococcus

Protein attributes

Sequence length	317 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	(S)-lactate + NAD+ = pyruvate + NADH. HAMAP MF_00488
Pathway	Fermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1. HAMAP MF_00488
Subunit structure	Homotetramer By similarity. HAMAP MF_00488
Subcellular location	Cytoplasm By similarity HAMAP MF_00488.
Sequence similarities	Belongs to the LDH/MDH superfamily. LDH family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Oxidoreductase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	L-lactate dehydrogenase activity Inferred from electronic annotation. Source: EC nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 317	317	L-lactate dehydrogenase 2 HAMAP MF_00488		PRO_0000168344
Regions
	Nucleotide binding	14 – 42	29	NAD By similarity
Sites
	Active site	178	1	Proton acceptor By similarity
	Binding site	91	1	Substrate By similarity
	Binding site	123	1	NAD or substrate By similarity
	Binding site	154	1	Substrate By similarity
	Binding site	232	1	Substrate By similarity
Amino acid modifications
	Modified residue	223	1	Phosphotyrosine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q838C9 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: D4C27C58A7D55E5A	FASTA	317	34,368
	10 20 30 40 50 60 MKVFNKKVAI IGTGFVGTSI AYSMINQGIA NELILVDIDK AKSEGEAIDL LDGVSWGQEN 70 80 90 100 110 120 VNVWAGDYQD CQDADIVVIT AGANQKPGQS RLDLVSINAE IMKTIVNNIM KSGFDGILVI 130 140 150 160 170 180 ASNPVDVLTY VAWQASGLPV SRVIGTGTTL DTTRFRKELS QRLAIDPRNV HGYIIGEHGD 190 200 210 220 230 240 SEVAVWSHTM IGTKPILEIV DTTERLTSDD LPIISDKVKN TAYEIIDRKQ ATYYGIGMST 250 260 270 280 290 300 ARIVKAILNN EQAILPVSAY LDGQYGQQDV FTGIPAVVGN QGVTDIIELN LNAAEKELFQ 310 KSVTQLKQVM ASLQPNA

References

[1]

"Role of mobile DNA in the evolution of vancomycin-resistant Enterococcus faecalis." Paulsen I.T., Banerjei L., Myers G.S.A., Nelson K.E., Seshadri R., Read T.D., Fouts D.E., Eisen J.A., Gill S.R., Heidelberg J.F., Tettelin H., Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J., Daugherty S.C., DeBoy R.T., Durkin S.A. , Kolonay J.F., Madupu R., Nelson W.C., Vamathevan J.J., Tran B., Upton J., Hansen T., Shetty J., Khouri H.M., Utterback T.R., Radune D., Ketchum K.A., Dougherty B.A., Fraser C.M. Science 299:2071-2074(2003) [PubMed: 12663927] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700802 / V583.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016830 Genomic DNA. Translation: AAO80466.1.
RefSeq	NP_814395.1. NC_004668.1.
3D structure databases
ProteinModelPortal	Q838C9.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1199542.
GenomeReviews	Gene locus EF_0641 in contig AE016830_GR.
KEGG	efa:EF0641.
NMPDR	fig|226185.1.peg.581.
PATRIC	21851676. VBIEntFae7065_0588.
TIGR	EF_0641.
Phylogenomic databases
HOGENOM	HBG566126.
OMA	GVSKEVC.
ProtClustDB	CLSK697192.
Enzyme and pathway databases
BioCyc	EFAE226185:EF_0641-MONOMER.
Family and domain databases
HAMAP	MF_00488. Lactate_dehydrog. [Tree]
InterPro	IPR001557. L-lactate/malate_DH. IPR011304. L-lactate_DH. IPR018177. L-lactate_DH_AS. IPR022383. Lactate/malate_DH_C. IPR001236. Lactate/malate_DH_N. IPR015955. Lactate_DH/Glyco_Ohase_4_C. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.90.110.10. lact_mal_DH. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K00016.
PANTHER	PTHR11540:SF3. PTHR11540:SF3. 1 hit.
Pfam	PF02866. Ldh_1_C. 1 hit. PF00056. Ldh_1_N. 1 hit. [Graphical view]
PIRSF	PIRSF000102. Lac_mal_DH. 1 hit.
PRINTS	PR00086. LLDHDRGNASE.
SUPFAM	SSF56327. Lactate_DH/Glyco_hydro_4_C. 1 hit.
TIGRFAMs	TIGR01771. L-LDH-NAD. 1 hit.
PROSITE	PS00064. L_LDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LDH2_ENTFA

Accession

Primary (citable) accession number: Q838C9

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 21, 2004
Last sequence update:	June 1, 2003
Last modified:	January 25, 2012
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program