Q83887 (GP_NYV) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Envelope glycoprotein
Short name=GP

Alternative name(s):
M polyprotein

Cleaved into the following 2 chains:

1. Glycoprotein G1
2. Glycoprotein G2

Gene names

Name:

GP

Organism

New York virus (NYV)

Taxonomic identifier

44755 [NCBI]

Taxonomic lineage

Viruses › ssRNA viruses › ssRNA negative-strand viruses › Bunyaviridae › Hantavirus

Virus host

Homo sapiens (Human) [TaxID: 9606]
Peromyscus leucopus (White-footed mouse) [TaxID: 10041]

Protein attributes

Sequence length	1140 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Glycoprotein G1 and glycoprotein G2 interact with each other and are present at the surface of the virion. They are able to attach the virion to a cell receptor and to promote fusion of membranes after endocytosis of the virion. G1 contains an ITAM motif which is likely to dysregulate normal immune and endothelial cell responses and contribute to virus pathogenesis.
Subunit structure	G1 and G2 interacts with each other By similarity. G1 interacts with human LYN, SYK and ZAP70. Ref.2
Subcellular location	Glycoprotein G1: Virion membrane; Single-pass type I membrane protein Potential. Host Golgi apparatus membrane; Single-pass type I membrane protein Potential. Host endoplasmic reticulum membrane; Single-pass type I membrane protein Potential. Note: Interaction between G1 and G2 is essential for proper targeting of G1 to the Golgi complex, where virion budding occurs By similarity. Glycoprotein G2: Virion membrane; Single-pass type I membrane protein Potential. Host Golgi apparatus membrane; Single-pass type I membrane protein Potential.
Post-translational modification	Envelope polyprotein precursor is quickly cleaved in vivo just after synthesis, presumably by host signal peptidase By similarity.
Sequence similarities	Belongs to the hantavirus envelope glycoprotein family. Contains 1 ITAM domain.

Ontologies

Keywords
Biological process	Fusion of virus membrane with host endosomal membrane Fusion of virus membrane with host membrane Host-virus interaction Inhibition of host IFN-mediated response initiation by virus Inhibition of host TRAFs by virus Inhibition of host innate immune response by virus Viral attachment to host cell Viral immunoevasion Viral penetration into host cytoplasm Virus entry into host cell
Cellular component	Host Golgi apparatus Host endoplasmic reticulum Host membrane Membrane Virion
Domain	Signal Transmembrane Transmembrane helix
PTM	Glycoprotein Phosphoprotein
Gene Ontology (GO)
Biological_process	signal transduction Inferred from electronic annotation. Source: InterPro suppression by virus of host TRAF activity Inferred from electronic annotation. Source: UniProtKB-KW suppression by virus of host innate immune response Inferred from electronic annotation. Source: UniProtKB-KW suppression by virus of host type I interferon production Inferred from electronic annotation. Source: UniProtKB-KW viral attachment to host cell Inferred from electronic annotation. Source: UniProtKB-KW viral entry into host cell Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	host cell Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell host cell endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW virion membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 17	17	Potential
	Chain	18 – 1140	1123	Envelope glycoprotein		PRO_0000235995
	Chain	18 – 652	635	Glycoprotein G1 By similarity		PRO_0000235996
	Chain	653 – 1140	488	Glycoprotein G2 By similarity		PRO_0000235997
Regions
	Topological domain	18 – 489	472	Lumenal Potential
	Transmembrane	490 – 510	21	Helical; Potential
	Topological domain	511 – 652	142	Cytoplasmic Potential
	Topological domain	653 – 1108	456	Lumenal Potential
	Transmembrane	1109 – 1129	21	Helical; Potential
	Topological domain	1130 – 1140	11	Cytoplasmic Potential
	Domain	615 – 638	24	ITAM
Sites
	Site	652 – 653	2	Cleavage; by host signal peptidase By similarity
Amino acid modifications
	Modified residue	619	1	Phosphotyrosine; by host By similarity
	Modified residue	632	1	Phosphotyrosine; by host By similarity
	Glycosylation	138	1	N-linked (GlcNAc...); by host Potential
	Glycosylation	351	1	N-linked (GlcNAc...); by host Potential
	Glycosylation	403	1	N-linked (GlcNAc...); by host Potential
	Glycosylation	931	1	N-linked (GlcNAc...); by host Potential
Natural variations
	Natural variant	4	1	W → F in strain: Isolate New York-2.
	Natural variant	8	1	S → F in strain: Isolate New York-2 and Isolate Rhode Island-1.
	Natural variant	46	1	E → G in strain: Isolate New York-2.
	Natural variant	141	1	H → Y in strain: Isolate Rhode Island-1.
	Natural variant	238	1	S → G in strain: Isolate New York-2.
	Natural variant	261	1	F → S in strain: Isolate Rhode Island-1.
	Natural variant	314	1	T → A in strain: Isolate New York-2.
	Natural variant	325	1	M → T in strain: Isolate Rhode Island-1.
	Natural variant	359	1	T → A in strain: Isolate Rhode Island-1.
	Natural variant	394	1	E → K in strain: Isolate Rhode Island-1.
	Natural variant	452	1	V → I in strain: Isolate Rhode Island-1.
	Natural variant	489	1	T → A in strain: Isolate Rhode Island-1.
	Natural variant	551	1	V → A in strain: Isolate Rhode Island-1.
	Natural variant	589	1	Q → L in strain: Isolate Rhode Island-1.
	Natural variant	618	1	C → R in strain: Isolate Rhode Island-1.
	Natural variant	697	1	N → D in strain: Isolate New York-2.
	Natural variant	794	1	V → G in strain: Isolate Rhode Island-1.
	Natural variant	1043	1	G → S in strain: Isolate Rhode Island-1.

Sequences

	Sequence		Length	Mass (Da)
	Q83887 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: BD3CCFDB0417AC42	FASTA	1,140	125,619
	10 20 30 40 50 60 MVGWVCISLV VLATTTAGLT RNLYELKIEC PHTVGLGQGY VTGSVETTPI LLTQVTDLKI 70 80 90 100 110 120 ESSCNFDLHV PSTSIQKYNQ VEWAKKSSTT ESTSAGATTF EAKTKEVSLK GTCNIPVTTF 130 140 150 160 170 180 EAAYKSRKTV ICYDLACNQT HCLPTVHLIA PVQTCMSVRS CMIGLLSSRI QVIYEKTYCV 190 200 210 220 230 240 TGQLVEGLCF IPTHTIALTQ PGHTYDTMTL PITCFLVAKK LGTQLKIAVE LEKLITASGC 250 260 270 280 290 300 TENSFQGYYI CFLGKHSEPL FVPMMDDYRS AELFTRMVLN PRGEDHDPDQ NGQGLMRIAG 310 320 330 340 350 360 PITAKVPSTE TTETMQGIAF AGAPMYSSFS TLVRKADPDY VFSPGIIAES NHSVCDKKTI 370 380 390 400 410 420 PLTWTGFLAV SGEIEKITGC TVFCTLVGPG ASCEAYSETG IFNISSPTCL VNKVQKFRGS 430 440 450 460 470 480 EQRINFMCQR VDQDVIVYCN GQKKVILTKT LVIGQCIYTF TSLFSLIPGV AHSLAVELCV 490 500 510 520 530 540 PGLHGWATTA LLITFCFGWL LIPTITMIIL KILRLLTFSC SHYSTESKFK AILERVKVEY 550 560 570 580 590 600 QKTMGSMVCD VCHHECETAK ELETHKKSCP EGQCPYCMTM TESTESALQA HFSICKLTNR 610 620 630 640 650 660 FQENLKKSLK RPEVKQGCYR TLGVFRYKSR CYVGLVWGVL LTTELIVWAA SADTPLMESG 670 680 690 700 710 720 WSDTAHGVGI VPMKTDLELD FALASSSSYS YRRKLVNPAN KEETLPFHFQ LDKQVVHAEI 730 740 750 760 770 780 QNLGHWMDGT FNIKTAFHCY GECKKYAYPW QTAKCFFEKD YQYETSWGCN PPDCPGVGTG 790 800 810 820 830 840 CTACGVYLDK LRSVGKAYKI VSLKFTRKVC IQLGTEQTCK HIDVNDCLVT PSVKVCLIGT 850 860 870 880 890 900 ISKLQPGDTL LFLGPLEQGG IILKQWCTTS CVFGDPGDIM STTTGMKCPE HTGSFRKICG 910 920 930 940 950 960 FATTPTCEYQ GNTISGFQRM MATRDSFQSF NVTEPHITSN RLEWIDPDSS IKDHINMVLN 970 980 990 1000 1010 1020 RDVSFQDLSD NPCKVDLHTQ SIDGAWGSGV GFTLVCTVGL TECANFITSI KACDSAMCYG 1030 1040 1050 1060 1070 1080 ATVTNLLRGS NTVKVVGKGG HSGSLFKCCH DTDCTEEGLA ASPPHLDRVT GYNQIDSDKV 1090 1100 1110 1120 1130 1140 YDDGAPPCTI KCWFTKSGEW LLGILNGNWV VVAVLIVILI LSILLFSFFC PIRGRKNKSN

References

[1]

"Molecular linkage of hantavirus pulmonary syndrome to the white-footed mouse, Peromyscus leucopus: genetic characterization of the M genome of New York virus." Hjelle B., Lee S.-W., Song W., Torrez-Martinez N., Song J.-W., Yanagihara R., Gavrilovskaya I.N., Mackow E.R. J. Virol. 69:8137-8141(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA]. Strain: Isolate New York-1, Isolate New York-2 and Isolate Rhode Island-1.

[2]

"Hantavirus pulmonary syndrome-associated hantaviruses contain conserved and functional ITAM signaling elements." Geimonen E., LaMonica R., Springer K., Farooqui Y., Gavrilovskaya I.N., Mackow E.R. J. Virol. 77:1638-1643(2003) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH HUMAN LYN; HUMAN SYK AND HUMAN ZAP70.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U36802 Genomic RNA. Translation: AAC54560.1. U36801 Genomic RNA. Translation: AAC54559.1. U36803 Genomic RNA. Translation: AAC54561.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR016402. Envelope_gylcoprot_Hantavirus. IPR002534. Hanta_G1. IPR002532. Hanta_G2. IPR012316. ITAM_motif_hantavir-typ. [Graphical view]
Pfam	PF01567. Hanta_G1. 1 hit. PF01561. Hanta_G2. 1 hit. PF10538. ITAM_Cys-rich. 1 hit. [Graphical view]
PIRSF	PIRSF003945. M_poly_HantaV. 1 hit.
ProDom	PD001813. Hanta_G2. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS51055. ITAM_1. False negative. PS51056. ITAM_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GP_NYV

Accession

Primary (citable) accession number: Q83887
Secondary accession number(s): Q83886, Q83888

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 16, 2006
Last sequence update:	November 1, 1996
Last modified:	April 3, 2013
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program