SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q83883

- POLG_NVN68

UniProt

Q83883 - POLG_NVN68

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Genome polyprotein
Gene
ORF1
Organism
Norwalk virus (strain GI/Human/United States/Norwalk/1968) (Hu/NV/NV/1968/US)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Protein p48 may play a role in viral replication by interacting with host VAPA, a vesicle-associated membrane protein that plays a role in SNARE-mediated vesicle fusion. This interaction may target replication complex to intracellular membranes.2 Publications
NTPase presumably plays a role in replication. Despite having similarities with helicases, does not seem to display any helicase activity.2 Publications
Protein P22 may play a role in targeting replication complex to intracellular membranes.2 Publications
Viral genome-linked protein is covalently linked to the 5'-end of the positive-strand, negative-strand genomic RNAs and subgenomic RNA. Acts as a genome-linked replication primer. May recruit ribosome to viral RNA thereby promoting viral proteins translation.2 Publications
3C-like protease processes the polyprotein: 3CLpro-RdRp is first released by autocleavage, then all other proteins are cleaved. May cleave host polyadenylate-binding protein thereby inhibiting cellular translation By similarity.2 Publications
RNA-directed RNA polymerase replicates genomic and antigenomic RNA by recognizing replications specific signals. Transcribes also a subgenomic mRNA by initiating RNA synthesis internally on antigenomic RNA. This sgRNA codes for structural proteins. Catalyzes the covalent attachment VPg with viral RNAs By similarity.2 Publications

Catalytic activityi

NTP + H2O = NDP + phosphate.
Endopeptidase with a preference for cleavage when the P1 position is occupied by Glu-|-Xaa and the P1' position is occupied by Gly-|-Yaa.
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei398 – 3992Cleavage; by 3CLpro
Sitei761 – 7622Cleavage; by 3CLpro
Sitei962 – 9632Cleavage; by 3CLpro
Sitei1100 – 11012Cleavage; by 3CLpro
Active sitei1130 – 11301For 3CLpro activity By similarity
Active sitei1154 – 11541For 3CLpro activity By similarity
Active sitei1239 – 12391For 3CLpro activity By similarity
Sitei1281 – 12822Cleavage; by 3CLpro

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi560 – 5678ATP Reviewed prediction

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. RNA binding Source: InterPro
  3. RNA helicase activity Source: InterPro
  4. RNA-directed RNA polymerase activity Source: UniProtKB-KW
  5. cysteine-type endopeptidase activity Source: InterPro

GO - Biological processi

  1. RNA-protein covalent cross-linking Source: UniProtKB-KW
  2. transcription, DNA-templated Source: InterPro
  3. viral RNA genome replication Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Nucleotidyltransferase, Protease, RNA-directed RNA polymerase, Thiol protease, Transferase

Keywords - Biological processi

Host-virus interaction, Viral RNA replication

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Protein family/group databases

MEROPSiC37.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Genome polyprotein
Cleaved into the following 6 chains:
Alternative name(s):
p41
Alternative name(s):
VPG
3C-like protease (EC:3.4.22.66)
Short name:
3CLpro
Alternative name(s):
Calicivirin
Gene namesi
ORF Names:ORF1
OrganismiNorwalk virus (strain GI/Human/United States/Norwalk/1968) (Hu/NV/NV/1968/US)
Taxonomic identifieri524364 [NCBI]
Taxonomic lineageiVirusesssRNA positive-strand viruses, no DNA stageCaliciviridaeNorovirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
ProteomesiUP000000826: Genome

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei359 – 37921Helical; Reviewed prediction
Add
BLAST
Transmembranei402 – 42221Helical; Reviewed prediction
Add
BLAST
Transmembranei870 – 89021Helical; Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. host cell membrane Source: UniProtKB-SubCell
  2. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Host membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi394 – 3941D → A, E or N: No effect on p48-p41 cleavage. 1 Publication
Mutagenesisi395 – 3951F → G: Complete loss of p48-p41 cleavage. 1 Publication
Mutagenesisi395 – 3951F → I or Y: No effect on p48-p41 cleavage. 1 Publication
Mutagenesisi398 – 3981Q → E or N: No effect on p48-p41 cleavage. 1 Publication
Mutagenesisi398 – 3981Q → G: Complete loss of p48-p41 cleavage. 1 Publication
Mutagenesisi399 – 3991G → A: No effect on p48-p41 cleavage. 1 Publication
Mutagenesisi1154 – 11541E → G: Complete loss of 3CLpro activity. 1 Publication
Mutagenesisi1167 – 11671D → G: No effect on 3CLpro activity. 1 Publication
Mutagenesisi1281 – 12811E → Q: No effect on 3CLpro activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 17891789Genome polyprotein
PRO_0000341617Add
BLAST
Chaini1 – 398398Protein p48
PRO_0000341618Add
BLAST
Chaini399 – 761363NTPase
PRO_0000341619Add
BLAST
Chaini762 – 962201Protein p22
PRO_0000341620Add
BLAST
Chaini963 – 1100138Viral genome-linked protein
PRO_0000341621Add
BLAST
Chaini1101 – 12811813C-like protease
PRO_0000341622Add
BLAST
Chaini1282 – 1789508RNA-directed RNA polymerase
PRO_0000341623Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei992 – 9921O-(5'-phospho-RNA)-tyrosine By similarity

Post-translational modificationi

Specific enzymatic cleavages in vivo yield mature proteins. 3CLpro is first autocatalytically cleaved, then processes the whole polyprotein.1 Publication
VPg is uridylylated by the polymerase and is covalently attached to the 5'-end of the polyadenylated genomic and subgenomic RNAs. This uridylylated form acts as a nucleotide-peptide primer for the polymerase By similarity.

Keywords - PTMi

Covalent protein-RNA linkage, Phosphoprotein

Interactioni

Subunit structurei

Protein p48 interacts with human VAPA.1 Publication

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi1103 – 11075
Beta strandi1109 – 11124
Beta strandi1115 – 112814
Helixi1129 – 11313
Beta strandi1137 – 11393
Helixi1144 – 11463
Beta strandi1147 – 11526
Beta strandi1155 – 11628
Beta strandi1182 – 11887
Beta strandi1190 – 11923
Beta strandi1194 – 120916
Beta strandi1212 – 122110
Beta strandi1223 – 12253
Beta strandi1229 – 12335
Helixi1236 – 12383
Beta strandi1242 – 12476
Beta strandi1250 – 126011
Beta strandi1262 – 12709
Beta strandi1275 – 12784

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2FYQX-ray1.50A1101-1281[»]
2FYRX-ray2.20A1101-1281[»]
2LNCNMR-A1101-1281[»]
3UR6X-ray1.50A/B1101-1281[»]
3UR9X-ray1.65A/B1101-1281[»]
4IMQX-ray1.50A1101-1281[»]
4IMZX-ray1.70A1101-1281[»]
4IN1X-ray2.05A1101-1281[»]
4IN2X-ray2.40A/B1100-1281[»]
4INHX-ray1.70A/B/C/D/E/F/G/H1101-1281[»]
ProteinModelPortaliQ83883.
SMRiQ83883. Positions 1101-1280, 1285-1787.

Miscellaneous databases

EvolutionaryTraceiQ83883.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini532 – 697166SF3 helicase
Add
BLAST
Domaini1101 – 1281181Peptidase C37
Add
BLAST
Domaini1516 – 1637122RdRp catalytic
Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR000605. Helicase_SF3_ssDNA/RNA_vir.
IPR014759. Helicase_SF3_ssRNA_vir.
IPR001665. Norovirus_pept_C37.
IPR027417. P-loop_NTPase.
IPR001205. RNA-dir_pol_C.
IPR007094. RNA-dir_pol_PSvirus.
IPR009003. Trypsin-like_Pept_dom.
IPR013614. Viral_PP_Calicivir_N.
[Graphical view]
PfamiPF08405. Calici_PP_N. 1 hit.
PF05416. Peptidase_C37. 1 hit.
PF00680. RdRP_1. 1 hit.
PF00910. RNA_helicase. 1 hit.
[Graphical view]
PRINTSiPR00917. SRSVCYSPTASE.
SUPFAMiSSF50494. SSF50494. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiPS51537. NV_3CL_PRO. 1 hit.
PS50507. RDRP_SSRNA_POS. 1 hit.
PS51218. SF3_HELICASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q83883-1 [UniParc]FASTAAdd to Basket

« Hide

MMMASKDVVP TAASSENANN NSSIKSRLLA RLKGSGGATS PPNSIKITNQ     50
DMALGLIGQV PAPKATSVDV PKQQRDRPPR TVAEVQQNLR WTERPQDQNV 100
KTWDELDHTT KQQILDEHAE WFDAGGLGPS TLPTSHERYT HENDEGHQVK 150
WSAREGVDLG ISGLTTVSGP EWNMCPLPPV DQRSTTPATE PTIGDMIEFY 200
EGHIYHYAIY IGQGKTVGVH SPQAAFSITR ITIQPISAWW RVCYVPQPKQ 250
RLTYDQLKEL ENEPWPYAAV TNNCFEFCCQ VMCLEDTWLQ RKLISSGRFY 300
HPTQDWSRDT PEFQQDSKLE MVRDAVLAAI NGLVSRPFKD LLGKLKPLNV 350
LNLLSNCDWT FMGVVEMVVL LLELFGIFWN PPDVSNFIAS LLPDFHLQGP 400
EDLARDLVPI VLGGIGLAIG FTRDKVSKMM KNAVDGLRAA TQLGQYGLEI 450
FSLLKKYFFG GDQTEKTLKD IESAVIDMEV LSSTSVTQLV RDKQSARAYM 500
AILDNEEEKA RKLSVRNADP HVVSSTNALI SRISMARAAL AKAQAEMTSR 550
MRPVVIMMCG PPGIGKTKAA EHLAKRLANE IRPGGKVGLV PREAVDHWDG 600
YHGEEVMLWD DYGMTKIQED CNKLQAIADS APLTLNCDRI ENKGMQFVSD 650
AIVITTNAPG PAPVDFVNLG PVCRRVDFLV YCTAPEVEHT RKVSPGDTTA 700
LKDCFKPDFS HLKMELAPQG GFDNQGNTPF GKGVMKPTTI NRLLIQAVAL 750
TMERQDEFQL QGPTYDFDTD RVAAFTRMAR ANGLGLISMA SLGKKLRSVT 800
TIEGLKNALS GYKISKCSIQ WQSRVYIIES DGASVQIKED KQALTPLQQT 850
INTASLAITR LKAARAVAYA SCFQSAITTI LQMAGSALVI NRAVKRMFGT 900
RTAAMALEGP GKEHNCRVHK AKEAGKGPIG HDDMVERFGL CETEEEESED 950
QIQMVPSDAV PEGKNKGKTK KGRGRKNNYN AFSRRGLSDE EYEEYKKIRE 1000
EKNGNYSIQE YLEDRQRYEE ELAEVQAGGD GGIGETEMEI RHRVFYKSKS 1050
KKHQQEQRRQ LGLVTGSDIR KRKPIDWTPP KNEWADDDRE VDYNEKINFE 1100
APPTLWSRVT KFGSGWGFWV SPTVFITTTH VVPTGVKEFF GEPLSSIAIH 1150
QAGEFTQFRF SKKMRPDLTG MVLEEGCPEG TVCSVLIKRD SGELLPLAVR 1200
MGAIASMRIQ GRLVHGQSGM LLTGANAKGM DLGTIPGDCG APYVHKRGND 1250
WVVCGVHAAA TKSGNTVVCA VQAGEGETAL EGGDKGHYAG HEIVRYGSGP 1300
ALSTKTKFWR SSPEPLPPGV YEPAYLGGKD PRVQNGPSLQ QVLRDQLKPF 1350
ADPRGRMPEP GLLEAAVETV TSMLEQTMDT PSPWSYADAC QSLDKTTSSG 1400
YPHHKRKNDD WNGTTFVGEL GEQAAHANNM YENAKHMKPI YTAALKDELV 1450
KPEKIYQKVK KRLLWGADLG TVVRAARAFG PFCDAIKSHV IKLPIKVGMN 1500
TIEDGPLIYA EHAKYKNHFD ADYTAWDSTQ NRQIMTESFS IMSRLTASPE 1550
LAEVVAQDLL APSEMDVGDY VIRVKEGLPS GFPCTSQVNS INHWIITLCA 1600
LSEATGLSPD VVQSMSYFSF YGDDEIVSTD IDFDPARLTQ ILKEYGLKPT 1650
RPDKTEGPIQ VRKNVDGLVF LRRTISRDAA GFQGRLDRAS IERQIFWTRG 1700
PNHSDPSETL VPHTQRKIQL ISLLGEASLH GEKFYRKISS KVIHEIKTGG 1750
LEMYVPGWQA MFRWMRFHDL GLWTGDRDLL PEFVNDDGV 1789
Length:1,789
Mass (Da):198,694
Last modified:November 1, 1996 - v1
Checksum:i6C84EF9EE62809B9
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M87661 Genomic RNA. Translation: AAB50465.1.
PIRiA53260.
C37471.
C53260.
D37471.
E37471.
RefSeqiNP_056820.1. NC_001959.2.

Genome annotation databases

GeneIDi1491970.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M87661 Genomic RNA. Translation: AAB50465.1 .
PIRi A53260.
C37471.
C53260.
D37471.
E37471.
RefSeqi NP_056820.1. NC_001959.2.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2FYQ X-ray 1.50 A 1101-1281 [» ]
2FYR X-ray 2.20 A 1101-1281 [» ]
2LNC NMR - A 1101-1281 [» ]
3UR6 X-ray 1.50 A/B 1101-1281 [» ]
3UR9 X-ray 1.65 A/B 1101-1281 [» ]
4IMQ X-ray 1.50 A 1101-1281 [» ]
4IMZ X-ray 1.70 A 1101-1281 [» ]
4IN1 X-ray 2.05 A 1101-1281 [» ]
4IN2 X-ray 2.40 A/B 1100-1281 [» ]
4INH X-ray 1.70 A/B/C/D/E/F/G/H 1101-1281 [» ]
ProteinModelPortali Q83883.
SMRi Q83883. Positions 1101-1280, 1285-1787.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

MEROPSi C37.001.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 1491970.

Miscellaneous databases

EvolutionaryTracei Q83883.

Family and domain databases

Gene3Di 3.40.50.300. 2 hits.
InterProi IPR000605. Helicase_SF3_ssDNA/RNA_vir.
IPR014759. Helicase_SF3_ssRNA_vir.
IPR001665. Norovirus_pept_C37.
IPR027417. P-loop_NTPase.
IPR001205. RNA-dir_pol_C.
IPR007094. RNA-dir_pol_PSvirus.
IPR009003. Trypsin-like_Pept_dom.
IPR013614. Viral_PP_Calicivir_N.
[Graphical view ]
Pfami PF08405. Calici_PP_N. 1 hit.
PF05416. Peptidase_C37. 1 hit.
PF00680. RdRP_1. 1 hit.
PF00910. RNA_helicase. 1 hit.
[Graphical view ]
PRINTSi PR00917. SRSVCYSPTASE.
SUPFAMi SSF50494. SSF50494. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEi PS51537. NV_3CL_PRO. 1 hit.
PS50507. RDRP_SSRNA_POS. 1 hit.
PS51218. SF3_HELICASE_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Sequence and genomic organization of Norwalk virus."
    Jiang X., Wang M., Wang K., Estes M.K.
    Virology 195:51-61(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA] OF 3-1789.
  2. "Completion of the Norwalk virus genome sequence."
    Hardy M.E., Estes M.K.
    Virus Genes 12:287-290(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA] OF 1-3.
  3. "Presence of a covalently linked protein on calicivirus RNA."
    Burroughs J.N., Brown F.
    J. Gen. Virol. 41:443-446(1978) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION OF VPG.
  4. "Polypeptide p41 of a Norwalk-like virus is a nucleic acid-independent nucleoside triphosphatase."
    Pfister T., Wimmer E.
    J. Virol. 75:1611-1619(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION OF NTPASE.
  5. "Substrate specificity of the Norwalk virus 3C-like proteinase."
    Hardy M.E., Crone T.J., Brower J.E., Ettayebi K.
    Virus Res. 89:29-39(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF ASP-394; PHE-395; GLN-398; GLY-399; GLU-1154; ASP-1167 AND GLU-1281.
  6. "Norwalk virus nonstructural protein p48 forms a complex with the SNARE regulator VAP-A and prevents cell surface expression of vesicular stomatitis virus G protein."
    Ettayebi K., Hardy M.E.
    J. Virol. 77:11790-11797(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH HUMAN VAPA.
  7. "Differential cleavage of the norovirus polyprotein precursor by two active forms of the viral protease."
    Scheffler U., Rudolph W., Gebhardt J., Rohayem J.
    J. Gen. Virol. 88:2013-2018(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEOLYTIC PROCESSING OF POLYPROTEIN.
  8. "Norovirus protein structure and function."
    Hardy M.E.
    FEMS Microbiol. Lett. 253:1-8(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.
  9. "X-ray crystallographic structure of the Norwalk virus protease at 1.5-A resolution."
    Zeitler C.E., Estes M.K., Venkataram Prasad B.V.
    J. Virol. 80:5050-5058(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS) OF 1100-1281.

Entry informationi

Entry nameiPOLG_NVN68
AccessioniPrimary (citable) accession number: Q83883
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 2008
Last sequence update: November 1, 1996
Last modified: September 3, 2014
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi