Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q83883

- POLG_NVN68

UniProt

Q83883 - POLG_NVN68

Protein

Genome polyprotein

Gene

ORF1

Organism
Norwalk virus (strain GI/Human/United States/Norwalk/1968) (Hu/NV/NV/1968/US)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 93 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Protein p48 may play a role in viral replication by interacting with host VAPA, a vesicle-associated membrane protein that plays a role in SNARE-mediated vesicle fusion. This interaction may target replication complex to intracellular membranes.
    NTPase presumably plays a role in replication. Despite having similarities with helicases, does not seem to display any helicase activity.
    Protein P22 may play a role in targeting replication complex to intracellular membranes.
    Viral genome-linked protein is covalently linked to the 5'-end of the positive-strand, negative-strand genomic RNAs and subgenomic RNA. Acts as a genome-linked replication primer. May recruit ribosome to viral RNA thereby promoting viral proteins translation.
    3C-like protease processes the polyprotein: 3CLpro-RdRp is first released by autocleavage, then all other proteins are cleaved. May cleave host polyadenylate-binding protein thereby inhibiting cellular translation By similarity.By similarity
    RNA-directed RNA polymerase replicates genomic and antigenomic RNA by recognizing replications specific signals. Transcribes also a subgenomic mRNA by initiating RNA synthesis internally on antigenomic RNA. This sgRNA codes for structural proteins. Catalyzes the covalent attachment VPg with viral RNAs By similarity.PROSITE-ProRule annotation

    Catalytic activityi

    NTP + H2O = NDP + phosphate.
    Endopeptidase with a preference for cleavage when the P1 position is occupied by Glu-|-Xaa and the P1' position is occupied by Gly-|-Yaa.PROSITE-ProRule annotation
    Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei398 – 3992Cleavage; by 3CLpro
    Sitei761 – 7622Cleavage; by 3CLpro
    Sitei962 – 9632Cleavage; by 3CLpro
    Sitei1100 – 11012Cleavage; by 3CLpro
    Active sitei1130 – 11301For 3CLpro activityPROSITE-ProRule annotation
    Active sitei1154 – 11541For 3CLpro activityPROSITE-ProRule annotation
    Active sitei1239 – 12391For 3CLpro activityPROSITE-ProRule annotation
    Sitei1281 – 12822Cleavage; by 3CLpro

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi560 – 5678ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. cysteine-type endopeptidase activity Source: InterPro
    3. RNA binding Source: InterPro
    4. RNA-directed RNA polymerase activity Source: UniProtKB-KW
    5. RNA helicase activity Source: InterPro

    GO - Biological processi

    1. RNA-protein covalent cross-linking Source: UniProtKB-KW
    2. transcription, DNA-templated Source: InterPro
    3. viral RNA genome replication Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Nucleotidyltransferase, Protease, RNA-directed RNA polymerase, Thiol protease, Transferase

    Keywords - Biological processi

    Host-virus interaction, Viral RNA replication

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Protein family/group databases

    MEROPSiC37.001.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Genome polyprotein
    Cleaved into the following 6 chains:
    Alternative name(s):
    p41
    Alternative name(s):
    VPG
    3C-like protease (EC:3.4.22.66)
    Short name:
    3CLpro
    Alternative name(s):
    Calicivirin
    Gene namesi
    ORF Names:ORF1
    OrganismiNorwalk virus (strain GI/Human/United States/Norwalk/1968) (Hu/NV/NV/1968/US)
    Taxonomic identifieri524364 [NCBI]
    Taxonomic lineageiVirusesssRNA positive-strand viruses, no DNA stageCaliciviridaeNorovirus
    Virus hostiHomo sapiens (Human) [TaxID: 9606]
    ProteomesiUP000000826: Genome

    Subcellular locationi

    GO - Cellular componenti

    1. host cell membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Host membrane, Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi394 – 3941D → A, E or N: No effect on p48-p41 cleavage. 1 Publication
    Mutagenesisi395 – 3951F → G: Complete loss of p48-p41 cleavage. 1 Publication
    Mutagenesisi395 – 3951F → I or Y: No effect on p48-p41 cleavage. 1 Publication
    Mutagenesisi398 – 3981Q → E or N: No effect on p48-p41 cleavage. 1 Publication
    Mutagenesisi398 – 3981Q → G: Complete loss of p48-p41 cleavage. 1 Publication
    Mutagenesisi399 – 3991G → A: No effect on p48-p41 cleavage. 1 Publication
    Mutagenesisi1154 – 11541E → G: Complete loss of 3CLpro activity. 1 Publication
    Mutagenesisi1167 – 11671D → G: No effect on 3CLpro activity. 1 Publication
    Mutagenesisi1281 – 12811E → Q: No effect on 3CLpro activity. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 17891789Genome polyproteinPRO_0000341617Add
    BLAST
    Chaini1 – 398398Protein p48PRO_0000341618Add
    BLAST
    Chaini399 – 761363NTPasePRO_0000341619Add
    BLAST
    Chaini762 – 962201Protein p22PRO_0000341620Add
    BLAST
    Chaini963 – 1100138Viral genome-linked proteinPRO_0000341621Add
    BLAST
    Chaini1101 – 12811813C-like proteasePRO_0000341622Add
    BLAST
    Chaini1282 – 1789508RNA-directed RNA polymerasePRO_0000341623Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei992 – 9921O-(5'-phospho-RNA)-tyrosineBy similarity

    Post-translational modificationi

    Specific enzymatic cleavages in vivo yield mature proteins. 3CLpro is first autocatalytically cleaved, then processes the whole polyprotein.1 PublicationPROSITE-ProRule annotation
    VPg is uridylylated by the polymerase and is covalently attached to the 5'-end of the polyadenylated genomic and subgenomic RNAs. This uridylylated form acts as a nucleotide-peptide primer for the polymerase By similarity.By similarity

    Keywords - PTMi

    Covalent protein-RNA linkage, Phosphoprotein

    Interactioni

    Subunit structurei

    Protein p48 interacts with human VAPA.1 Publication

    Structurei

    Secondary structure

    1
    1789
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi1103 – 11075
    Beta strandi1109 – 11124
    Beta strandi1115 – 112814
    Helixi1129 – 11313
    Beta strandi1137 – 11393
    Helixi1144 – 11463
    Beta strandi1147 – 11526
    Beta strandi1155 – 11628
    Beta strandi1182 – 11887
    Beta strandi1190 – 11923
    Beta strandi1194 – 120916
    Beta strandi1212 – 122110
    Beta strandi1223 – 12253
    Beta strandi1229 – 12335
    Helixi1236 – 12383
    Beta strandi1242 – 12476
    Beta strandi1250 – 126011
    Beta strandi1262 – 12709
    Beta strandi1275 – 12784

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2FYQX-ray1.50A1101-1281[»]
    2FYRX-ray2.20A1101-1281[»]
    2LNCNMR-A1101-1281[»]
    3UR6X-ray1.50A/B1101-1281[»]
    3UR9X-ray1.65A/B1101-1281[»]
    4IMQX-ray1.50A1101-1281[»]
    4IMZX-ray1.70A1101-1281[»]
    4IN1X-ray2.05A1101-1281[»]
    4IN2X-ray2.40A/B1100-1281[»]
    4INHX-ray1.70A/B/C/D/E/F/G/H1101-1281[»]
    ProteinModelPortaliQ83883.
    SMRiQ83883. Positions 1101-1280, 1285-1787.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ83883.

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei359 – 37921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei402 – 42221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei870 – 89021HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini532 – 697166SF3 helicasePROSITE-ProRule annotationAdd
    BLAST
    Domaini1101 – 1281181Peptidase C37PROSITE-ProRule annotationAdd
    BLAST
    Domaini1516 – 1637122RdRp catalyticPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 peptidase C37 domain.PROSITE-ProRule annotation
    Contains 1 RdRp catalytic domain.PROSITE-ProRule annotation
    Contains 1 SF3 helicase domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Family and domain databases

    Gene3Di3.40.50.300. 2 hits.
    InterProiIPR000605. Helicase_SF3_ssDNA/RNA_vir.
    IPR014759. Helicase_SF3_ssRNA_vir.
    IPR001665. Norovirus_pept_C37.
    IPR027417. P-loop_NTPase.
    IPR001205. RNA-dir_pol_C.
    IPR007094. RNA-dir_pol_PSvirus.
    IPR009003. Trypsin-like_Pept_dom.
    IPR013614. Viral_PP_Calicivir_N.
    [Graphical view]
    PfamiPF08405. Calici_PP_N. 1 hit.
    PF05416. Peptidase_C37. 1 hit.
    PF00680. RdRP_1. 1 hit.
    PF00910. RNA_helicase. 1 hit.
    [Graphical view]
    PRINTSiPR00917. SRSVCYSPTASE.
    SUPFAMiSSF50494. SSF50494. 1 hit.
    SSF52540. SSF52540. 1 hit.
    PROSITEiPS51537. NV_3CL_PRO. 1 hit.
    PS50507. RDRP_SSRNA_POS. 1 hit.
    PS51218. SF3_HELICASE_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q83883-1 [UniParc]FASTAAdd to Basket

    « Hide

    MMMASKDVVP TAASSENANN NSSIKSRLLA RLKGSGGATS PPNSIKITNQ     50
    DMALGLIGQV PAPKATSVDV PKQQRDRPPR TVAEVQQNLR WTERPQDQNV 100
    KTWDELDHTT KQQILDEHAE WFDAGGLGPS TLPTSHERYT HENDEGHQVK 150
    WSAREGVDLG ISGLTTVSGP EWNMCPLPPV DQRSTTPATE PTIGDMIEFY 200
    EGHIYHYAIY IGQGKTVGVH SPQAAFSITR ITIQPISAWW RVCYVPQPKQ 250
    RLTYDQLKEL ENEPWPYAAV TNNCFEFCCQ VMCLEDTWLQ RKLISSGRFY 300
    HPTQDWSRDT PEFQQDSKLE MVRDAVLAAI NGLVSRPFKD LLGKLKPLNV 350
    LNLLSNCDWT FMGVVEMVVL LLELFGIFWN PPDVSNFIAS LLPDFHLQGP 400
    EDLARDLVPI VLGGIGLAIG FTRDKVSKMM KNAVDGLRAA TQLGQYGLEI 450
    FSLLKKYFFG GDQTEKTLKD IESAVIDMEV LSSTSVTQLV RDKQSARAYM 500
    AILDNEEEKA RKLSVRNADP HVVSSTNALI SRISMARAAL AKAQAEMTSR 550
    MRPVVIMMCG PPGIGKTKAA EHLAKRLANE IRPGGKVGLV PREAVDHWDG 600
    YHGEEVMLWD DYGMTKIQED CNKLQAIADS APLTLNCDRI ENKGMQFVSD 650
    AIVITTNAPG PAPVDFVNLG PVCRRVDFLV YCTAPEVEHT RKVSPGDTTA 700
    LKDCFKPDFS HLKMELAPQG GFDNQGNTPF GKGVMKPTTI NRLLIQAVAL 750
    TMERQDEFQL QGPTYDFDTD RVAAFTRMAR ANGLGLISMA SLGKKLRSVT 800
    TIEGLKNALS GYKISKCSIQ WQSRVYIIES DGASVQIKED KQALTPLQQT 850
    INTASLAITR LKAARAVAYA SCFQSAITTI LQMAGSALVI NRAVKRMFGT 900
    RTAAMALEGP GKEHNCRVHK AKEAGKGPIG HDDMVERFGL CETEEEESED 950
    QIQMVPSDAV PEGKNKGKTK KGRGRKNNYN AFSRRGLSDE EYEEYKKIRE 1000
    EKNGNYSIQE YLEDRQRYEE ELAEVQAGGD GGIGETEMEI RHRVFYKSKS 1050
    KKHQQEQRRQ LGLVTGSDIR KRKPIDWTPP KNEWADDDRE VDYNEKINFE 1100
    APPTLWSRVT KFGSGWGFWV SPTVFITTTH VVPTGVKEFF GEPLSSIAIH 1150
    QAGEFTQFRF SKKMRPDLTG MVLEEGCPEG TVCSVLIKRD SGELLPLAVR 1200
    MGAIASMRIQ GRLVHGQSGM LLTGANAKGM DLGTIPGDCG APYVHKRGND 1250
    WVVCGVHAAA TKSGNTVVCA VQAGEGETAL EGGDKGHYAG HEIVRYGSGP 1300
    ALSTKTKFWR SSPEPLPPGV YEPAYLGGKD PRVQNGPSLQ QVLRDQLKPF 1350
    ADPRGRMPEP GLLEAAVETV TSMLEQTMDT PSPWSYADAC QSLDKTTSSG 1400
    YPHHKRKNDD WNGTTFVGEL GEQAAHANNM YENAKHMKPI YTAALKDELV 1450
    KPEKIYQKVK KRLLWGADLG TVVRAARAFG PFCDAIKSHV IKLPIKVGMN 1500
    TIEDGPLIYA EHAKYKNHFD ADYTAWDSTQ NRQIMTESFS IMSRLTASPE 1550
    LAEVVAQDLL APSEMDVGDY VIRVKEGLPS GFPCTSQVNS INHWIITLCA 1600
    LSEATGLSPD VVQSMSYFSF YGDDEIVSTD IDFDPARLTQ ILKEYGLKPT 1650
    RPDKTEGPIQ VRKNVDGLVF LRRTISRDAA GFQGRLDRAS IERQIFWTRG 1700
    PNHSDPSETL VPHTQRKIQL ISLLGEASLH GEKFYRKISS KVIHEIKTGG 1750
    LEMYVPGWQA MFRWMRFHDL GLWTGDRDLL PEFVNDDGV 1789
    Length:1,789
    Mass (Da):198,694
    Last modified:November 1, 1996 - v1
    Checksum:i6C84EF9EE62809B9
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M87661 Genomic RNA. Translation: AAB50465.1.
    PIRiA53260.
    C37471.
    C53260.
    D37471.
    E37471.
    RefSeqiNP_056820.1. NC_001959.2.

    Genome annotation databases

    GeneIDi1491970.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M87661 Genomic RNA. Translation: AAB50465.1 .
    PIRi A53260.
    C37471.
    C53260.
    D37471.
    E37471.
    RefSeqi NP_056820.1. NC_001959.2.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2FYQ X-ray 1.50 A 1101-1281 [» ]
    2FYR X-ray 2.20 A 1101-1281 [» ]
    2LNC NMR - A 1101-1281 [» ]
    3UR6 X-ray 1.50 A/B 1101-1281 [» ]
    3UR9 X-ray 1.65 A/B 1101-1281 [» ]
    4IMQ X-ray 1.50 A 1101-1281 [» ]
    4IMZ X-ray 1.70 A 1101-1281 [» ]
    4IN1 X-ray 2.05 A 1101-1281 [» ]
    4IN2 X-ray 2.40 A/B 1100-1281 [» ]
    4INH X-ray 1.70 A/B/C/D/E/F/G/H 1101-1281 [» ]
    ProteinModelPortali Q83883.
    SMRi Q83883. Positions 1101-1280, 1285-1787.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    MEROPSi C37.001.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 1491970.

    Miscellaneous databases

    EvolutionaryTracei Q83883.

    Family and domain databases

    Gene3Di 3.40.50.300. 2 hits.
    InterProi IPR000605. Helicase_SF3_ssDNA/RNA_vir.
    IPR014759. Helicase_SF3_ssRNA_vir.
    IPR001665. Norovirus_pept_C37.
    IPR027417. P-loop_NTPase.
    IPR001205. RNA-dir_pol_C.
    IPR007094. RNA-dir_pol_PSvirus.
    IPR009003. Trypsin-like_Pept_dom.
    IPR013614. Viral_PP_Calicivir_N.
    [Graphical view ]
    Pfami PF08405. Calici_PP_N. 1 hit.
    PF05416. Peptidase_C37. 1 hit.
    PF00680. RdRP_1. 1 hit.
    PF00910. RNA_helicase. 1 hit.
    [Graphical view ]
    PRINTSi PR00917. SRSVCYSPTASE.
    SUPFAMi SSF50494. SSF50494. 1 hit.
    SSF52540. SSF52540. 1 hit.
    PROSITEi PS51537. NV_3CL_PRO. 1 hit.
    PS50507. RDRP_SSRNA_POS. 1 hit.
    PS51218. SF3_HELICASE_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence and genomic organization of Norwalk virus."
      Jiang X., Wang M., Wang K., Estes M.K.
      Virology 195:51-61(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA] OF 3-1789.
    2. "Completion of the Norwalk virus genome sequence."
      Hardy M.E., Estes M.K.
      Virus Genes 12:287-290(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA] OF 1-3.
    3. "Presence of a covalently linked protein on calicivirus RNA."
      Burroughs J.N., Brown F.
      J. Gen. Virol. 41:443-446(1978) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION OF VPG.
    4. "Polypeptide p41 of a Norwalk-like virus is a nucleic acid-independent nucleoside triphosphatase."
      Pfister T., Wimmer E.
      J. Virol. 75:1611-1619(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION OF NTPASE.
    5. "Substrate specificity of the Norwalk virus 3C-like proteinase."
      Hardy M.E., Crone T.J., Brower J.E., Ettayebi K.
      Virus Res. 89:29-39(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: MUTAGENESIS OF ASP-394; PHE-395; GLN-398; GLY-399; GLU-1154; ASP-1167 AND GLU-1281.
    6. "Norwalk virus nonstructural protein p48 forms a complex with the SNARE regulator VAP-A and prevents cell surface expression of vesicular stomatitis virus G protein."
      Ettayebi K., Hardy M.E.
      J. Virol. 77:11790-11797(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH HUMAN VAPA.
    7. "Differential cleavage of the norovirus polyprotein precursor by two active forms of the viral protease."
      Scheffler U., Rudolph W., Gebhardt J., Rohayem J.
      J. Gen. Virol. 88:2013-2018(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEOLYTIC PROCESSING OF POLYPROTEIN.
    8. "Norovirus protein structure and function."
      Hardy M.E.
      FEMS Microbiol. Lett. 253:1-8(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: REVIEW.
    9. "X-ray crystallographic structure of the Norwalk virus protease at 1.5-A resolution."
      Zeitler C.E., Estes M.K., Venkataram Prasad B.V.
      J. Virol. 80:5050-5058(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS) OF 1100-1281.

    Entry informationi

    Entry nameiPOLG_NVN68
    AccessioniPrimary (citable) accession number: Q83883
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 2008
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 93 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3