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Protein

DNA ligase

Gene

ligA

Organism
Enterococcus faecalis (strain ATCC 700802 / V583)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA.UniRule annotation

Catalytic activityi

NAD+ + (deoxyribonucleotide)(n)-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)(m) = (deoxyribonucleotide)(n+m) + AMP + beta-nicotinamide D-nucleotide.UniRule annotation

Cofactori

Mg2+UniRule annotation, Mn2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei118NADUniRule annotation1 Publication1
Active sitei120N6-AMP-lysine intermediateUniRule annotation1
Binding sitei141NADUniRule annotation1 Publication1
Binding sitei175NADUniRule annotation1 Publication1
Binding sitei291NADUniRule annotation1 Publication1
Binding sitei315NADUniRule annotation1 Publication1
Metal bindingi409ZincUniRule annotation1
Metal bindingi412ZincUniRule annotation1
Metal bindingi427ZincUniRule annotation1
Metal bindingi432ZincUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi39 – 43NADUniRule annotation1 Publication5
Nucleotide bindingi88 – 91NADUniRule annotation1 Publication4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processDNA damage, DNA repair, DNA replication
LigandMagnesium, Manganese, Metal-binding, NAD, Zinc

Enzyme and pathway databases

BioCyciEFAE226185:G1G0C-709-MONOMER
BRENDAi6.5.1.2 2095

Names & Taxonomyi

Protein namesi
Recommended name:
DNA ligaseUniRule annotation (EC:6.5.1.2UniRule annotation)
Alternative name(s):
Polydeoxyribonucleotide synthase [NAD(+)]UniRule annotation
Gene namesi
Name:ligAUniRule annotation
Ordered Locus Names:EF_0722
OrganismiEnterococcus faecalis (strain ATCC 700802 / V583)
Taxonomic identifieri226185 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesEnterococcaceaeEnterococcus
Proteomesi
  • UP000001415 Componenti: Chromosome

Pathology & Biotechi

Chemistry databases

DrugBankiDB03227 Nicotinamide Mononucleotide

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003132281 – 676DNA ligaseAdd BLAST676

Proteomic databases

PRIDEiQ837V6

Interactioni

Protein-protein interaction databases

STRINGi226185.EF0722

Structurei

Secondary structure

1676
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi8 – 30Combined sources23
Beta strandi31 – 33Combined sources3
Helixi39 – 55Combined sources17
Helixi57 – 59Combined sources3
Helixi65 – 67Combined sources3
Beta strandi79 – 81Combined sources3
Helixi95 – 109Combined sources15
Beta strandi115 – 121Combined sources7
Beta strandi123 – 131Combined sources9
Beta strandi134 – 140Combined sources7
Beta strandi144 – 149Combined sources6
Helixi151 – 155Combined sources5
Beta strandi165 – 167Combined sources3
Beta strandi170 – 177Combined sources8
Helixi180 – 192Combined sources13
Helixi201 – 209Combined sources9
Helixi214 – 219Combined sources6
Beta strandi223 – 230Combined sources8
Helixi240 – 250Combined sources11
Beta strandi259 – 263Combined sources5
Helixi264 – 277Combined sources14
Helixi278 – 280Combined sources3
Beta strandi281 – 283Combined sources3
Beta strandi285 – 294Combined sources10
Helixi295 – 301Combined sources7
Beta strandi305 – 315Combined sources11
Helixi319 – 321Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1TA8X-ray1.80A1-323[»]
1TAEX-ray2.70A/B/C/D1-324[»]
3BA8X-ray1.90A1-324[»]
3BA9X-ray1.90A1-324[»]
3BAAX-ray1.90A1-324[»]
3BABX-ray2.50A1-324[»]
4EEQX-ray2.10A1-323[»]
4EFBX-ray2.20A1-323[»]
4EFEX-ray2.00A1-323[»]
4LH6X-ray1.65A1-323[»]
4LH7X-ray1.90A1-323[»]
ProteinModelPortaliQ837V6
SMRiQ837V6
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ837V6

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini595 – 676BRCTUniRule annotationAdd BLAST82

Sequence similaritiesi

Belongs to the NAD-dependent DNA ligase family. LigA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C77 Bacteria
COG0272 LUCA
KOiK01972
OMAiFTAKSPR

Family and domain databases

CDDicd00027 BRCT, 1 hit
cd00114 LIGANc, 1 hit
Gene3Di3.40.50.10190, 1 hit
HAMAPiMF_01588 DNA_ligase_A, 1 hit
InterProiView protein in InterPro
IPR001357 BRCT_dom
IPR036420 BRCT_dom_sf
IPR018239 DNA_ligase_AS
IPR033136 DNA_ligase_CS
IPR001679 DNAligase
IPR013839 DNAligase_adenylation
IPR013840 DNAligase_N
IPR012340 NA-bd_OB-fold
IPR004150 NAD_DNA_ligase_OB
IPR010994 RuvA_2-like
IPR004149 Znf_DNAligase_C4
PfamiView protein in Pfam
PF00533 BRCT, 1 hit
PF01653 DNA_ligase_aden, 1 hit
PF03120 DNA_ligase_OB, 1 hit
PF03119 DNA_ligase_ZBD, 1 hit
PIRSFiPIRSF001604 LigA, 1 hit
SMARTiView protein in SMART
SM00292 BRCT, 1 hit
SM00532 LIGANc, 1 hit
SUPFAMiSSF47781 SSF47781, 1 hit
SSF50249 SSF50249, 1 hit
SSF52113 SSF52113, 1 hit
TIGRFAMsiTIGR00575 dnlj, 1 hit
PROSITEiView protein in PROSITE
PS50172 BRCT, 1 hit
PS01055 DNA_LIGASE_N1, 1 hit
PS01056 DNA_LIGASE_N2, 1 hit

Sequencei

Sequence statusi: Complete.

Q837V6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEQQPLTLTA ATTRAQELRK QLNQYSHEYY VKDQPSVEDY VYDRLYKELV
60 70 80 90 100
DIETEFPDLI TPDSPTQRVG GKVLSGFEKA PHDIPMYSLN DGFSKEDIFA
110 120 130 140 150
FDERVRKAIG KPVAYCCELK IDGLAISLRY ENGVFVRGAT RGDGTVGENI
160 170 180 190 200
TENLRTVRSV PMRLTEPISV EVRGECYMPK QSFVALNEER EENGQDIFAN
210 220 230 240 250
PRNAAAGSLR QLDTKIVAKR NLNTFLYTVA DFGPMKAKTQ FEALEELSAI
260 270 280 290 300
GFRTNPERQL CQSIDEVWAY IEEYHEKRST LPYEIDGIVI KVNEFALQDE
310 320 330 340 350
LGFTVKAPRW AIAYKFPPEE AETVVEDIEW TIGRTGVVTP TAVMAPVRVA
360 370 380 390 400
GTTVSRASLH NADFIQMKDI RLNDHVIIYK AGDIIPEVAQ VLVEKRAADS
410 420 430 440 450
QPYEMPTHCP ICHSELVHLD EEVALRCINP KCPAQIKEGL NHFVSRNAMN
460 470 480 490 500
IDGLGPRVLA QMYDKGLVKD VADLYFLTEE QLMTLDKIKE KSANNIYTAI
510 520 530 540 550
QGSKENSVER LIFGLGIRHV GAKAAKILAE HFGDLPTLSR ATAEEIVALD
560 570 580 590 600
SIGETIADSV VTYFENEEVH ELMAELEKAQ VNLTYKGLRT EQLAEVESPF
610 620 630 640 650
KDKTVVLTGK LAQYTREEAK EKIENLGGKV TGSVSKKTDI VVAGEDAGSK
660 670
LTKAESLGVT VWNEQEMVDA LDASHF
Length:676
Mass (Da):75,584
Last modified:June 1, 2003 - v1
Checksum:i9B6B93FD8DBB8E3B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016830 Genomic DNA Translation: AAO80542.1
RefSeqiNP_814472.1, NC_004668.1
WP_002361258.1, NZ_KE136527.1

Genome annotation databases

EnsemblBacteriaiAAO80542; AAO80542; EF_0722
GeneIDi1199621
KEGGiefa:EF0722
PATRICifig|226185.9.peg.667

Similar proteinsi

Entry informationi

Entry nameiDNLJ_ENTFA
AccessioniPrimary (citable) accession number: Q837V6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: June 1, 2003
Last modified: May 23, 2018
This is version 111 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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