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Q837U7

- PTC_ENTFA

UniProt

Q837U7 - PTC_ENTFA

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Protein

Putrescine carbamoyltransferase

Gene

ptcA

Organism
Enterococcus faecalis (strain ATCC 700802 / V583)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the phosphorolysis of N-carbamoylputrescine to form carbamoyl phosphate and putrescine. Is involved in the degradation pathway of the polyamine agmatine. Also has weak activity with ornithine and cadaverine.1 Publication

Catalytic activityi

Carbamoyl phosphate + putrescine = phosphate + N-carbamoylputrescine.1 Publication

Enzyme regulationi

Inhibited by spermidine and N-(phosphonoacetyl)-putrescine.2 Publications

Kineticsi

  1. KM=58 µM for carbamoyl phosphate1 Publication
  2. KM=2.3 mM for putrescine1 Publication

pH dependencei

Optimum pH is 6.7 with 10 mM putrescine and 10 mM carbamoyl phosphate, 7.8 with 0.2 mM putrescine and 0.2 mM carbamoyl phosphate, and 9.0 with 0.5 mM putrescine and 10 mM carbamoyl phosphate.1 Publication

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei27 – 271Important for structural integrityBy similarity
Binding sitei103 – 1031Carbamoyl phosphateBy similarity
Binding sitei130 – 1301Carbamoyl phosphateBy similarity
Sitei143 – 1431Important for structural integrityBy similarity

GO - Molecular functioni

  1. amino acid binding Source: InterPro
  2. putrescine carbamoyltransferase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. putrescine biosynthetic process from arginine via N-carbamoylputrescine Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Polyamine biosynthesis

Enzyme and pathway databases

BioCyciEFAE226185:GHI1-718-MONOMER.
SABIO-RKQ837U7.
UniPathwayiUPA00534; UER00941.

Names & Taxonomyi

Protein namesi
Recommended name:
Putrescine carbamoyltransferase (EC:2.1.3.6)
Short name:
PTC
Short name:
PTCase
Alternative name(s):
Agmatine catabolism protein B
Putrescine transcarbamoylase
Putrescine transcarbamylase
Gene namesi
Name:ptcA
Synonyms:agcB, argF-2
Ordered Locus Names:EF_0732
OrganismiEnterococcus faecalis (strain ATCC 700802 / V583)
Taxonomic identifieri226185 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesEnterococcaceaeEnterococcus
ProteomesiUP000001415: Chromosome

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 339339Putrescine carbamoyltransferasePRO_0000112923Add
BLAST

Interactioni

Subunit structurei

Homotrimer.2 Publications

Protein-protein interaction databases

STRINGi226185.EF0732.

Structurei

Secondary structure

1
339
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi8 – 103Combined sources
Helixi13 – 3119Combined sources
Turni38 – 414Combined sources
Beta strandi43 – 508Combined sources
Helixi54 – 6512Combined sources
Beta strandi69 – 735Combined sources
Turni75 – 773Combined sources
Beta strandi80 – 845Combined sources
Helixi86 – 9611Combined sources
Beta strandi98 – 1036Combined sources
Helixi107 – 11610Combined sources
Beta strandi121 – 1233Combined sources
Helixi131 – 14313Combined sources
Helixi151 – 1533Combined sources
Beta strandi155 – 1617Combined sources
Helixi164 – 17512Combined sources
Beta strandi179 – 1835Combined sources
Helixi186 – 1883Combined sources
Helixi192 – 20514Combined sources
Beta strandi208 – 2125Combined sources
Helixi215 – 2184Combined sources
Beta strandi222 – 2265Combined sources
Beta strandi229 – 2324Combined sources
Helixi239 – 2468Combined sources
Turni247 – 2504Combined sources
Helixi254 – 2607Combined sources
Beta strandi265 – 2684Combined sources
Turni275 – 2773Combined sources
Helixi280 – 2834Combined sources
Helixi290 – 31526Combined sources
Helixi320 – 33617Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3TXXX-ray3.20A/B/C/D/E/F/G/H/I/J/K/L1-339[»]
4A8HX-ray2.50A/B1-339[»]
4A8PX-ray2.00A/B/C/D/E/F1-339[»]
4A8TX-ray1.59A1-317[»]
4AM8X-ray1.99A/B/C/D/E/F1-339[»]
ProteinModelPortaliQ837U7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni52 – 565Carbamoyl phosphate bindingBy similarity
Regioni268 – 2714Putrescine bindingBy similarity

Sequence similaritiesi

Belongs to the ATCase/OTCase family. PTCase subfamily.Curated

Phylogenomic databases

eggNOGiCOG0078.
KOiK13252.
OMAiTMVEHLP.
OrthoDBiEOG690MGV.

Family and domain databases

Gene3Di3.40.50.1370. 2 hits.
HAMAPiMF_02102. PTCase.
InterProiIPR006132. Asp/Orn_carbamoyltranf_P-bd.
IPR006130. Asp/Orn_carbamoylTrfase.
IPR006131. Asp_carbamoyltransf_Asp/Orn-bd.
IPR002292. Orn/put_carbamltrans.
IPR024903. PtcA.
[Graphical view]
PfamiPF00185. OTCace. 1 hit.
PF02729. OTCace_N. 1 hit.
[Graphical view]
PRINTSiPR00100. AOTCASE.
PR00102. OTCASE.
SUPFAMiSSF53671. SSF53671. 1 hit.
TIGRFAMsiTIGR00658. orni_carb_tr. 1 hit.

Sequencei

Sequence statusi: Complete.

Q837U7-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKRDYVTTET YTKEEMHYLV DLSLKIKEAI KNGYYPQLLK NKSLGMIFQQ
60 70 80 90 100
SSTRTRVSFE TAMEQLGGHG EYLAPGQIQL GGHETIEDTS RVLSRLVDIL
110 120 130 140 150
MARVERHHSI VDLANCATIP VINGMSDYNH PTQELGDLCT MVEHLPEGKK
160 170 180 190 200
LEDCKVVFVG DATQVCFSLG LITTKMGMNF VHFGPEGFQL NEEHQAKLAK
210 220 230 240 250
NCEVSGGSFL VTDDASSVEG ADFLYTDVWY GLYEAELSEE ERMKVFYPKY
260 270 280 290 300
QVNQEMMDRA GANCKFMHCL PATRGEEVTD EVIDGKNSIC FDEAENRLTS
310 320 330
IRGLLVYLMN DYEAKNPYDL IKQAEAKKEL EVFLDTQSI
Length:339
Mass (Da):38,343
Last modified:June 1, 2003 - v1
Checksum:i1459EE8AC906CF96
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016830 Genomic DNA. Translation: AAO80551.1.
RefSeqiNP_814481.1. NC_004668.1.

Genome annotation databases

EnsemblBacteriaiAAO80551; AAO80551; EF_0732.
GeneIDi1199630.
KEGGiefa:EF0732.
PATRICi21851854. VBIEntFae7065_0677.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016830 Genomic DNA. Translation: AAO80551.1 .
RefSeqi NP_814481.1. NC_004668.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3TXX X-ray 3.20 A/B/C/D/E/F/G/H/I/J/K/L 1-339 [» ]
4A8H X-ray 2.50 A/B 1-339 [» ]
4A8P X-ray 2.00 A/B/C/D/E/F 1-339 [» ]
4A8T X-ray 1.59 A 1-317 [» ]
4AM8 X-ray 1.99 A/B/C/D/E/F 1-339 [» ]
ProteinModelPortali Q837U7.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 226185.EF0732.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAO80551 ; AAO80551 ; EF_0732 .
GeneIDi 1199630.
KEGGi efa:EF0732.
PATRICi 21851854. VBIEntFae7065_0677.

Phylogenomic databases

eggNOGi COG0078.
KOi K13252.
OMAi TMVEHLP.
OrthoDBi EOG690MGV.

Enzyme and pathway databases

UniPathwayi UPA00534 ; UER00941 .
BioCyci EFAE226185:GHI1-718-MONOMER.
SABIO-RK Q837U7.

Family and domain databases

Gene3Di 3.40.50.1370. 2 hits.
HAMAPi MF_02102. PTCase.
InterProi IPR006132. Asp/Orn_carbamoyltranf_P-bd.
IPR006130. Asp/Orn_carbamoylTrfase.
IPR006131. Asp_carbamoyltransf_Asp/Orn-bd.
IPR002292. Orn/put_carbamltrans.
IPR024903. PtcA.
[Graphical view ]
Pfami PF00185. OTCace. 1 hit.
PF02729. OTCace_N. 1 hit.
[Graphical view ]
PRINTSi PR00100. AOTCASE.
PR00102. OTCASE.
SUPFAMi SSF53671. SSF53671. 1 hit.
TIGRFAMsi TIGR00658. orni_carb_tr. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700802 / V583.
  2. "The gene cluster for agmatine catabolism of Enterococcus faecalis: study of recombinant putrescine transcarbamylase and agmatine deiminase and a snapshot of agmatine deiminase catalyzing its reaction."
    Llacer J.L., Polo L.M., Tavarez S., Alarcon B., Hilario R., Rubio V.
    J. Bacteriol. 189:1254-1265(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 1-14, SUBUNIT, ENZYME REGULATION, CRYSTALLIZATION, KINETIC PARAMETERS.
    Strain: SD10.
  3. "Evolutionary relationships among bacterial carbamoyltransferases."
    Tricot C., De Coen J.-L., Momin P., Falmagne P., Stalon V.
    J. Gen. Microbiol. 135:2453-2464(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-30.
    Strain: ATCC 11700 / DSM 20409 / NCIMB 8661 / 10C1.
  4. "Fermentation of agmatine in Streptococcus faecalis: occurrence of putrescine transcarbamoylase."
    Roon R.J., Barker H.A.
    J. Bacteriol. 109:44-50(1972) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY.
    Strain: ATCC 11700 / DSM 20409 / NCIMB 8661 / 10C1.
  5. "Structure and properties of the putrescine carbamoyltransferase of Streptococcus faecalis."
    Wargnies B., Lauwers N., Stalon V.
    Eur. J. Biochem. 101:143-152(1979) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBSTRATE SPECIFICITY, SUBUNIT, PH DEPENDENCE, ENZYME REGULATION.
  6. "The difficulty of annotating genes: the case of putrescine carbamoyltransferase."
    Naumoff D.G., Xu Y., Stalon V., Glansdorff N., Labedan B.
    Microbiology 150:3908-3911(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE NAME.

Entry informationi

Entry nameiPTC_ENTFA
AccessioniPrimary (citable) accession number: Q837U7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 7, 2004
Last sequence update: June 1, 2003
Last modified: November 26, 2014
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3