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Protein

Putrescine carbamoyltransferase

Gene

ptcA

Organism
Enterococcus faecalis (strain ATCC 700802 / V583)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the phosphorolysis of N-carbamoylputrescine to form carbamoyl phosphate and putrescine. Is involved in the degradation pathway of the polyamine agmatine. Also has weak activity with ornithine and cadaverine.1 Publication

Catalytic activityi

Carbamoyl phosphate + putrescine = phosphate + N-carbamoylputrescine.1 Publication

Enzyme regulationi

Inhibited by spermidine and N-(phosphonoacetyl)-putrescine.2 Publications

Kineticsi

  1. KM=58 µM for carbamoyl phosphate1 Publication
  2. KM=2.3 mM for putrescine1 Publication

    pH dependencei

    Optimum pH is 6.7 with 10 mM putrescine and 10 mM carbamoyl phosphate, 7.8 with 0.2 mM putrescine and 0.2 mM carbamoyl phosphate, and 9.0 with 0.5 mM putrescine and 10 mM carbamoyl phosphate.1 Publication

    Pathwayi: putrescine biosynthesis via agmatine pathway

    This protein is involved in step 1 of the subpathway that synthesizes putrescine from N-carbamoylputrescine (transferase route).
    Proteins known to be involved in this subpathway in this organism are:
    1. Putrescine carbamoyltransferase (ptcA)
    This subpathway is part of the pathway putrescine biosynthesis via agmatine pathway, which is itself part of Amine and polyamine biosynthesis.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes putrescine from N-carbamoylputrescine (transferase route), the pathway putrescine biosynthesis via agmatine pathway and in Amine and polyamine biosynthesis.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei103Carbamoyl phosphateBy similarity1
    Binding sitei130Carbamoyl phosphateBy similarity1

    GO - Molecular functioni

    GO - Biological processi

    Keywordsi

    Molecular functionTransferase
    Biological processPolyamine biosynthesis

    Enzyme and pathway databases

    BioCyciEFAE226185:G1G0C-718-MONOMER
    BRENDAi2.1.3.3 2095
    2.1.3.6 2095
    SABIO-RKiQ837U7
    UniPathwayiUPA00534; UER00941

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Putrescine carbamoyltransferase (EC:2.1.3.6)
    Short name:
    PTC
    Short name:
    PTCase
    Alternative name(s):
    Agmatine catabolism protein B
    Putrescine transcarbamoylase
    Putrescine transcarbamylase
    Gene namesi
    Name:ptcA
    Synonyms:agcB, argF-2
    Ordered Locus Names:EF_0732
    OrganismiEnterococcus faecalis (strain ATCC 700802 / V583)
    Taxonomic identifieri226185 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesEnterococcaceaeEnterococcus
    Proteomesi
    • UP000001415 Componenti: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001129231 – 339Putrescine carbamoyltransferaseAdd BLAST339

    Proteomic databases

    PRIDEiQ837U7

    Interactioni

    Subunit structurei

    Homotrimer.2 Publications

    Protein-protein interaction databases

    STRINGi226185.EF0732

    Structurei

    Secondary structure

    1339
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Helixi8 – 10Combined sources3
    Helixi13 – 31Combined sources19
    Turni38 – 41Combined sources4
    Beta strandi43 – 50Combined sources8
    Helixi54 – 65Combined sources12
    Beta strandi69 – 73Combined sources5
    Turni75 – 77Combined sources3
    Beta strandi80 – 84Combined sources5
    Helixi86 – 96Combined sources11
    Beta strandi98 – 103Combined sources6
    Helixi107 – 116Combined sources10
    Beta strandi121 – 123Combined sources3
    Helixi131 – 143Combined sources13
    Helixi151 – 153Combined sources3
    Beta strandi155 – 161Combined sources7
    Helixi164 – 175Combined sources12
    Beta strandi179 – 183Combined sources5
    Helixi186 – 188Combined sources3
    Helixi192 – 205Combined sources14
    Beta strandi208 – 212Combined sources5
    Helixi215 – 218Combined sources4
    Beta strandi222 – 226Combined sources5
    Beta strandi229 – 232Combined sources4
    Helixi239 – 246Combined sources8
    Turni247 – 250Combined sources4
    Helixi254 – 260Combined sources7
    Beta strandi265 – 268Combined sources4
    Turni275 – 277Combined sources3
    Helixi280 – 283Combined sources4
    Helixi290 – 315Combined sources26
    Helixi320 – 335Combined sources16

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sitei27Important for structural integrityBy similarity1
    Sitei143Important for structural integrityBy similarity1

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    3TXXX-ray3.20A/B/C/D/E/F/G/H/I/J/K/L1-339[»]
    4A8HX-ray2.50A/B1-339[»]
    4A8PX-ray2.00A/B/C/D/E/F1-339[»]
    4A8TX-ray1.59A1-317[»]
    4AM8X-ray1.99A/B/C/D/E/F1-339[»]
    ProteinModelPortaliQ837U7
    SMRiQ837U7
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni52 – 56Carbamoyl phosphate bindingBy similarity5
    Regioni268 – 271Putrescine bindingBy similarity4

    Sequence similaritiesi

    Belongs to the ATCase/OTCase family. PTCase subfamily.Curated

    Phylogenomic databases

    eggNOGiENOG4105DBV Bacteria
    COG0078 LUCA
    KOiK13252
    OMAiRMAIFYP

    Family and domain databases

    Gene3Di3.40.50.1370, 3 hits
    HAMAPiMF_02102 PTCase, 1 hit
    InterProiView protein in InterPro
    IPR006132 Asp/Orn_carbamoyltranf_P-bd
    IPR006130 Asp/Orn_carbamoylTrfase
    IPR036901 Asp/Orn_carbamoylTrfase_sf
    IPR006131 Asp_carbamoyltransf_Asp/Orn-bd
    IPR002292 Orn/put_carbamltrans
    IPR024903 PtcA
    PfamiView protein in Pfam
    PF00185 OTCace, 1 hit
    PF02729 OTCace_N, 1 hit
    PRINTSiPR00100 AOTCASE
    PR00102 OTCASE
    SUPFAMiSSF53671 SSF53671, 1 hit
    TIGRFAMsiTIGR00658 orni_carb_tr, 1 hit
    TIGR04384 putr_carbamoyl, 1 hit

    Sequencei

    Sequence statusi: Complete.

    Q837U7-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MKRDYVTTET YTKEEMHYLV DLSLKIKEAI KNGYYPQLLK NKSLGMIFQQ
    60 70 80 90 100
    SSTRTRVSFE TAMEQLGGHG EYLAPGQIQL GGHETIEDTS RVLSRLVDIL
    110 120 130 140 150
    MARVERHHSI VDLANCATIP VINGMSDYNH PTQELGDLCT MVEHLPEGKK
    160 170 180 190 200
    LEDCKVVFVG DATQVCFSLG LITTKMGMNF VHFGPEGFQL NEEHQAKLAK
    210 220 230 240 250
    NCEVSGGSFL VTDDASSVEG ADFLYTDVWY GLYEAELSEE ERMKVFYPKY
    260 270 280 290 300
    QVNQEMMDRA GANCKFMHCL PATRGEEVTD EVIDGKNSIC FDEAENRLTS
    310 320 330
    IRGLLVYLMN DYEAKNPYDL IKQAEAKKEL EVFLDTQSI
    Length:339
    Mass (Da):38,343
    Last modified:June 1, 2003 - v1
    Checksum:i1459EE8AC906CF96
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AE016830 Genomic DNA Translation: AAO80551.1
    RefSeqiNP_814481.1, NC_004668.1
    WP_002355587.1, NZ_KE136527.1

    Genome annotation databases

    EnsemblBacteriaiAAO80551; AAO80551; EF_0732
    GeneIDi1199630
    KEGGiefa:EF0732
    PATRICifig|226185.45.peg.2673

    Similar proteinsi

    Entry informationi

    Entry nameiPTC_ENTFA
    AccessioniPrimary (citable) accession number: Q837U7
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 7, 2004
    Last sequence update: June 1, 2003
    Last modified: May 23, 2018
    This is version 101 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

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