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Protein

Putrescine carbamoyltransferase

Gene

ptcA

Organism
Enterococcus faecalis (strain ATCC 700802 / V583)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the phosphorolysis of N-carbamoylputrescine to form carbamoyl phosphate and putrescine. Is involved in the degradation pathway of the polyamine agmatine. Also has weak activity with ornithine and cadaverine.1 Publication

Catalytic activityi

Carbamoyl phosphate + putrescine = phosphate + N-carbamoylputrescine.1 Publication

Enzyme regulationi

Inhibited by spermidine and N-(phosphonoacetyl)-putrescine.2 Publications

Kineticsi

  1. KM=58 µM for carbamoyl phosphate1 Publication
  2. KM=2.3 mM for putrescine1 Publication

    pH dependencei

    Optimum pH is 6.7 with 10 mM putrescine and 10 mM carbamoyl phosphate, 7.8 with 0.2 mM putrescine and 0.2 mM carbamoyl phosphate, and 9.0 with 0.5 mM putrescine and 10 mM carbamoyl phosphate.1 Publication

    Pathwayi: putrescine biosynthesis via agmatine pathway

    This protein is involved in step 1 of the subpathway that synthesizes putrescine from N-carbamoylputrescine (transferase route).
    Proteins known to be involved in this subpathway in this organism are:
    1. Putrescine carbamoyltransferase (ptcA)
    This subpathway is part of the pathway putrescine biosynthesis via agmatine pathway, which is itself part of Amine and polyamine biosynthesis.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes putrescine from N-carbamoylputrescine (transferase route), the pathway putrescine biosynthesis via agmatine pathway and in Amine and polyamine biosynthesis.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei103Carbamoyl phosphateBy similarity1
    Binding sitei130Carbamoyl phosphateBy similarity1

    GO - Molecular functioni

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Polyamine biosynthesis

    Enzyme and pathway databases

    BRENDAi2.1.3.3. 2095.
    2.1.3.6. 2095.
    SABIO-RKQ837U7.
    UniPathwayiUPA00534; UER00941.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Putrescine carbamoyltransferase (EC:2.1.3.6)
    Short name:
    PTC
    Short name:
    PTCase
    Alternative name(s):
    Agmatine catabolism protein B
    Putrescine transcarbamoylase
    Putrescine transcarbamylase
    Gene namesi
    Name:ptcA
    Synonyms:agcB, argF-2
    Ordered Locus Names:EF_0732
    OrganismiEnterococcus faecalis (strain ATCC 700802 / V583)
    Taxonomic identifieri226185 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesEnterococcaceaeEnterococcus
    Proteomesi
    • UP000001415 Componenti: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    Complete GO annotation...

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001129231 – 339Putrescine carbamoyltransferaseAdd BLAST339

    Interactioni

    Subunit structurei

    Homotrimer.2 Publications

    Protein-protein interaction databases

    STRINGi226185.EF0732.

    Structurei

    Secondary structure

    1339
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Helixi8 – 10Combined sources3
    Helixi13 – 31Combined sources19
    Turni38 – 41Combined sources4
    Beta strandi43 – 50Combined sources8
    Helixi54 – 65Combined sources12
    Beta strandi69 – 73Combined sources5
    Turni75 – 77Combined sources3
    Beta strandi80 – 84Combined sources5
    Helixi86 – 96Combined sources11
    Beta strandi98 – 103Combined sources6
    Helixi107 – 116Combined sources10
    Beta strandi121 – 123Combined sources3
    Helixi131 – 143Combined sources13
    Helixi151 – 153Combined sources3
    Beta strandi155 – 161Combined sources7
    Helixi164 – 175Combined sources12
    Beta strandi179 – 183Combined sources5
    Helixi186 – 188Combined sources3
    Helixi192 – 205Combined sources14
    Beta strandi208 – 212Combined sources5
    Helixi215 – 218Combined sources4
    Beta strandi222 – 226Combined sources5
    Beta strandi229 – 232Combined sources4
    Helixi239 – 246Combined sources8
    Turni247 – 250Combined sources4
    Helixi254 – 260Combined sources7
    Beta strandi265 – 268Combined sources4
    Turni275 – 277Combined sources3
    Helixi280 – 283Combined sources4
    Helixi290 – 315Combined sources26
    Helixi320 – 336Combined sources17

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sitei27Important for structural integrityBy similarity1
    Sitei143Important for structural integrityBy similarity1

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    3TXXX-ray3.20A/B/C/D/E/F/G/H/I/J/K/L1-339[»]
    4A8HX-ray2.50A/B1-339[»]
    4A8PX-ray2.00A/B/C/D/E/F1-339[»]
    4A8TX-ray1.59A1-317[»]
    4AM8X-ray1.99A/B/C/D/E/F1-339[»]
    ProteinModelPortaliQ837U7.
    SMRiQ837U7.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni52 – 56Carbamoyl phosphate bindingBy similarity5
    Regioni268 – 271Putrescine bindingBy similarity4

    Sequence similaritiesi

    Belongs to the ATCase/OTCase family. PTCase subfamily.Curated

    Phylogenomic databases

    eggNOGiENOG4105DBV. Bacteria.
    COG0078. LUCA.
    KOiK13252.
    OMAiSAREHPC.

    Family and domain databases

    Gene3Di3.40.50.1370. 2 hits.
    HAMAPiMF_02102. PTCase. 1 hit.
    InterProiIPR006132. Asp/Orn_carbamoyltranf_P-bd.
    IPR006130. Asp/Orn_carbamoylTrfase.
    IPR006131. Asp_carbamoyltransf_Asp/Orn-bd.
    IPR002292. Orn/put_carbamltrans.
    IPR024903. PtcA.
    [Graphical view]
    PfamiPF00185. OTCace. 1 hit.
    PF02729. OTCace_N. 1 hit.
    [Graphical view]
    PRINTSiPR00100. AOTCASE.
    PR00102. OTCASE.
    SUPFAMiSSF53671. SSF53671. 1 hit.
    TIGRFAMsiTIGR00658. orni_carb_tr. 1 hit.
    TIGR04384. putr_carbamoyl. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q837U7-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MKRDYVTTET YTKEEMHYLV DLSLKIKEAI KNGYYPQLLK NKSLGMIFQQ
    60 70 80 90 100
    SSTRTRVSFE TAMEQLGGHG EYLAPGQIQL GGHETIEDTS RVLSRLVDIL
    110 120 130 140 150
    MARVERHHSI VDLANCATIP VINGMSDYNH PTQELGDLCT MVEHLPEGKK
    160 170 180 190 200
    LEDCKVVFVG DATQVCFSLG LITTKMGMNF VHFGPEGFQL NEEHQAKLAK
    210 220 230 240 250
    NCEVSGGSFL VTDDASSVEG ADFLYTDVWY GLYEAELSEE ERMKVFYPKY
    260 270 280 290 300
    QVNQEMMDRA GANCKFMHCL PATRGEEVTD EVIDGKNSIC FDEAENRLTS
    310 320 330
    IRGLLVYLMN DYEAKNPYDL IKQAEAKKEL EVFLDTQSI
    Length:339
    Mass (Da):38,343
    Last modified:June 1, 2003 - v1
    Checksum:i1459EE8AC906CF96
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AE016830 Genomic DNA. Translation: AAO80551.1.
    RefSeqiNP_814481.1. NC_004668.1.
    WP_002355587.1. NZ_KE136527.1.

    Genome annotation databases

    EnsemblBacteriaiAAO80551; AAO80551; EF_0732.
    GeneIDi1199630.
    KEGGiefa:EF0732.
    PATRICi21851854. VBIEntFae7065_0677.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AE016830 Genomic DNA. Translation: AAO80551.1.
    RefSeqiNP_814481.1. NC_004668.1.
    WP_002355587.1. NZ_KE136527.1.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    3TXXX-ray3.20A/B/C/D/E/F/G/H/I/J/K/L1-339[»]
    4A8HX-ray2.50A/B1-339[»]
    4A8PX-ray2.00A/B/C/D/E/F1-339[»]
    4A8TX-ray1.59A1-317[»]
    4AM8X-ray1.99A/B/C/D/E/F1-339[»]
    ProteinModelPortaliQ837U7.
    SMRiQ837U7.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi226185.EF0732.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiAAO80551; AAO80551; EF_0732.
    GeneIDi1199630.
    KEGGiefa:EF0732.
    PATRICi21851854. VBIEntFae7065_0677.

    Phylogenomic databases

    eggNOGiENOG4105DBV. Bacteria.
    COG0078. LUCA.
    KOiK13252.
    OMAiSAREHPC.

    Enzyme and pathway databases

    UniPathwayiUPA00534; UER00941.
    BRENDAi2.1.3.3. 2095.
    2.1.3.6. 2095.
    SABIO-RKQ837U7.

    Family and domain databases

    Gene3Di3.40.50.1370. 2 hits.
    HAMAPiMF_02102. PTCase. 1 hit.
    InterProiIPR006132. Asp/Orn_carbamoyltranf_P-bd.
    IPR006130. Asp/Orn_carbamoylTrfase.
    IPR006131. Asp_carbamoyltransf_Asp/Orn-bd.
    IPR002292. Orn/put_carbamltrans.
    IPR024903. PtcA.
    [Graphical view]
    PfamiPF00185. OTCace. 1 hit.
    PF02729. OTCace_N. 1 hit.
    [Graphical view]
    PRINTSiPR00100. AOTCASE.
    PR00102. OTCASE.
    SUPFAMiSSF53671. SSF53671. 1 hit.
    TIGRFAMsiTIGR00658. orni_carb_tr. 1 hit.
    TIGR04384. putr_carbamoyl. 1 hit.
    ProtoNetiSearch...

    Entry informationi

    Entry nameiPTC_ENTFA
    AccessioniPrimary (citable) accession number: Q837U7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 7, 2004
    Last sequence update: June 1, 2003
    Last modified: November 2, 2016
    This is version 95 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.