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Q837U7

- PTC_ENTFA

UniProt

Q837U7 - PTC_ENTFA

Protein

Putrescine carbamoyltransferase

Gene

ptcA

Organism
Enterococcus faecalis (strain ATCC 700802 / V583)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 86 (01 Oct 2014)
      Sequence version 1 (01 Jun 2003)
      Previous versions | rss
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    Functioni

    Catalyzes the phosphorolysis of N-carbamoylputrescine to form carbamoyl phosphate and putrescine. Is involved in the degradation pathway of the polyamine agmatine. Also has weak activity with ornithine and cadaverine.1 Publication

    Catalytic activityi

    Carbamoyl phosphate + putrescine = phosphate + N-carbamoylputrescine.1 Publication

    Enzyme regulationi

    Inhibited by spermidine and N-(phosphonoacetyl)-putrescine.2 Publications

    Kineticsi

    1. KM=58 µM for carbamoyl phosphate1 Publication
    2. KM=2.3 mM for putrescine1 Publication

    pH dependencei

    Optimum pH is 6.7 with 10 mM putrescine and 10 mM carbamoyl phosphate, 7.8 with 0.2 mM putrescine and 0.2 mM carbamoyl phosphate, and 9.0 with 0.5 mM putrescine and 10 mM carbamoyl phosphate.1 Publication

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei27 – 271Important for structural integrityBy similarity
    Binding sitei103 – 1031Carbamoyl phosphateBy similarity
    Binding sitei130 – 1301Carbamoyl phosphateBy similarity
    Sitei143 – 1431Important for structural integrityBy similarity

    GO - Molecular functioni

    1. amino acid binding Source: InterPro
    2. putrescine carbamoyltransferase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. putrescine biosynthetic process from arginine via N-carbamoylputrescine Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Polyamine biosynthesis

    Enzyme and pathway databases

    BioCyciEFAE226185:GHI1-718-MONOMER.
    SABIO-RKQ837U7.
    UniPathwayiUPA00534; UER00941.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Putrescine carbamoyltransferase (EC:2.1.3.6)
    Short name:
    PTC
    Short name:
    PTCase
    Alternative name(s):
    Agmatine catabolism protein B
    Putrescine transcarbamoylase
    Putrescine transcarbamylase
    Gene namesi
    Name:ptcA
    Synonyms:agcB, argF-2
    Ordered Locus Names:EF_0732
    OrganismiEnterococcus faecalis (strain ATCC 700802 / V583)
    Taxonomic identifieri226185 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesEnterococcaceaeEnterococcus
    ProteomesiUP000001415: Chromosome

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 339339Putrescine carbamoyltransferasePRO_0000112923Add
    BLAST

    Interactioni

    Subunit structurei

    Homotrimer.2 Publications

    Protein-protein interaction databases

    STRINGi226185.EF0732.

    Structurei

    Secondary structure

    1
    339
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi8 – 103
    Helixi13 – 3119
    Turni38 – 414
    Beta strandi43 – 508
    Helixi54 – 6512
    Beta strandi69 – 735
    Turni75 – 773
    Beta strandi80 – 845
    Helixi86 – 9611
    Beta strandi98 – 1036
    Helixi107 – 11610
    Beta strandi121 – 1233
    Helixi131 – 14313
    Helixi151 – 1533
    Beta strandi155 – 1617
    Helixi164 – 17512
    Beta strandi179 – 1835
    Helixi186 – 1883
    Helixi192 – 20514
    Beta strandi208 – 2125
    Helixi215 – 2184
    Beta strandi222 – 2265
    Beta strandi229 – 2324
    Helixi239 – 2468
    Turni247 – 2504
    Helixi254 – 2607
    Beta strandi265 – 2684
    Turni275 – 2773
    Helixi280 – 2834
    Helixi290 – 31526
    Helixi320 – 33617

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3TXXX-ray3.20A/B/C/D/E/F/G/H/I/J/K/L1-339[»]
    4A8HX-ray2.50A/B1-339[»]
    4A8PX-ray2.00A/B/C/D/E/F1-339[»]
    4A8TX-ray1.59A1-317[»]
    4AM8X-ray1.99A/B/C/D/E/F1-339[»]
    ProteinModelPortaliQ837U7.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni52 – 565Carbamoyl phosphate bindingBy similarity
    Regioni268 – 2714Putrescine bindingBy similarity

    Sequence similaritiesi

    Belongs to the ATCase/OTCase family. PTCase subfamily.Curated

    Phylogenomic databases

    eggNOGiCOG0078.
    KOiK13252.
    OMAiTMVEHLP.
    OrthoDBiEOG690MGV.

    Family and domain databases

    Gene3Di3.40.50.1370. 2 hits.
    HAMAPiMF_02102. PTCase.
    InterProiIPR006132. Asp/Orn_carbamoyltranf_P-bd.
    IPR006130. Asp/Orn_carbamoylTrfase.
    IPR006131. Asp_carbamoyltransf_Asp/Orn-bd.
    IPR002292. Orn/put_carbamltrans.
    IPR024903. PtcA.
    [Graphical view]
    PfamiPF00185. OTCace. 1 hit.
    PF02729. OTCace_N. 1 hit.
    [Graphical view]
    PRINTSiPR00100. AOTCASE.
    PR00102. OTCASE.
    SUPFAMiSSF53671. SSF53671. 1 hit.
    TIGRFAMsiTIGR00658. orni_carb_tr. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q837U7-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKRDYVTTET YTKEEMHYLV DLSLKIKEAI KNGYYPQLLK NKSLGMIFQQ    50
    SSTRTRVSFE TAMEQLGGHG EYLAPGQIQL GGHETIEDTS RVLSRLVDIL 100
    MARVERHHSI VDLANCATIP VINGMSDYNH PTQELGDLCT MVEHLPEGKK 150
    LEDCKVVFVG DATQVCFSLG LITTKMGMNF VHFGPEGFQL NEEHQAKLAK 200
    NCEVSGGSFL VTDDASSVEG ADFLYTDVWY GLYEAELSEE ERMKVFYPKY 250
    QVNQEMMDRA GANCKFMHCL PATRGEEVTD EVIDGKNSIC FDEAENRLTS 300
    IRGLLVYLMN DYEAKNPYDL IKQAEAKKEL EVFLDTQSI 339
    Length:339
    Mass (Da):38,343
    Last modified:June 1, 2003 - v1
    Checksum:i1459EE8AC906CF96
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE016830 Genomic DNA. Translation: AAO80551.1.
    RefSeqiNP_814481.1. NC_004668.1.

    Genome annotation databases

    EnsemblBacteriaiAAO80551; AAO80551; EF_0732.
    GeneIDi1199630.
    KEGGiefa:EF0732.
    PATRICi21851854. VBIEntFae7065_0677.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE016830 Genomic DNA. Translation: AAO80551.1 .
    RefSeqi NP_814481.1. NC_004668.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3TXX X-ray 3.20 A/B/C/D/E/F/G/H/I/J/K/L 1-339 [» ]
    4A8H X-ray 2.50 A/B 1-339 [» ]
    4A8P X-ray 2.00 A/B/C/D/E/F 1-339 [» ]
    4A8T X-ray 1.59 A 1-317 [» ]
    4AM8 X-ray 1.99 A/B/C/D/E/F 1-339 [» ]
    ProteinModelPortali Q837U7.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 226185.EF0732.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAO80551 ; AAO80551 ; EF_0732 .
    GeneIDi 1199630.
    KEGGi efa:EF0732.
    PATRICi 21851854. VBIEntFae7065_0677.

    Phylogenomic databases

    eggNOGi COG0078.
    KOi K13252.
    OMAi TMVEHLP.
    OrthoDBi EOG690MGV.

    Enzyme and pathway databases

    UniPathwayi UPA00534 ; UER00941 .
    BioCyci EFAE226185:GHI1-718-MONOMER.
    SABIO-RK Q837U7.

    Family and domain databases

    Gene3Di 3.40.50.1370. 2 hits.
    HAMAPi MF_02102. PTCase.
    InterProi IPR006132. Asp/Orn_carbamoyltranf_P-bd.
    IPR006130. Asp/Orn_carbamoylTrfase.
    IPR006131. Asp_carbamoyltransf_Asp/Orn-bd.
    IPR002292. Orn/put_carbamltrans.
    IPR024903. PtcA.
    [Graphical view ]
    Pfami PF00185. OTCace. 1 hit.
    PF02729. OTCace_N. 1 hit.
    [Graphical view ]
    PRINTSi PR00100. AOTCASE.
    PR00102. OTCASE.
    SUPFAMi SSF53671. SSF53671. 1 hit.
    TIGRFAMsi TIGR00658. orni_carb_tr. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 700802 / V583.
    2. "The gene cluster for agmatine catabolism of Enterococcus faecalis: study of recombinant putrescine transcarbamylase and agmatine deiminase and a snapshot of agmatine deiminase catalyzing its reaction."
      Llacer J.L., Polo L.M., Tavarez S., Alarcon B., Hilario R., Rubio V.
      J. Bacteriol. 189:1254-1265(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 1-14, SUBUNIT, ENZYME REGULATION, CRYSTALLIZATION, KINETIC PARAMETERS.
      Strain: SD10.
    3. "Evolutionary relationships among bacterial carbamoyltransferases."
      Tricot C., De Coen J.-L., Momin P., Falmagne P., Stalon V.
      J. Gen. Microbiol. 135:2453-2464(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 2-30.
      Strain: ATCC 11700 / DSM 20409 / NCIMB 8661 / 10C1.
    4. "Fermentation of agmatine in Streptococcus faecalis: occurrence of putrescine transcarbamoylase."
      Roon R.J., Barker H.A.
      J. Bacteriol. 109:44-50(1972) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY.
      Strain: ATCC 11700 / DSM 20409 / NCIMB 8661 / 10C1.
    5. "Structure and properties of the putrescine carbamoyltransferase of Streptococcus faecalis."
      Wargnies B., Lauwers N., Stalon V.
      Eur. J. Biochem. 101:143-152(1979) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBSTRATE SPECIFICITY, SUBUNIT, PH DEPENDENCE, ENZYME REGULATION.
    6. "The difficulty of annotating genes: the case of putrescine carbamoyltransferase."
      Naumoff D.G., Xu Y., Stalon V., Glansdorff N., Labedan B.
      Microbiology 150:3908-3911(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE NAME.

    Entry informationi

    Entry nameiPTC_ENTFA
    AccessioniPrimary (citable) accession number: Q837U7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 7, 2004
    Last sequence update: June 1, 2003
    Last modified: October 1, 2014
    This is version 86 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3