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Q837N5 (SYL_ENTFA) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 74. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:EF_0801
OrganismEnterococcus faecalis (strain ATCC 700802 / V583) [Reference proteome] [HAMAP]
Taxonomic identifier226185 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesEnterococcaceaeEnterococcus

Protein attributes

Sequence length804 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 804804Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_0000152015

Regions

Motif40 – 5112"HIGH" region HAMAP-Rule MF_00049
Motif576 – 5805"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site5791ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q837N5 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: C79970F0DDFC14B5

FASTA80491,839
        10         20         30         40         50         60 
MSYNHKEIEK KWQKYWAKNN CFNTLDDPNK EKFYALDMFP YPSGQGLHVG HPEGYTATDI 

        70         80         90        100        110        120 
LSRMKRAQGY NVLHPMGWDA FGLPAEQYAL DTGNDPAEFT KKNIETFRRQ INSLGFSYDW 

       130        140        150        160        170        180 
NREINTTDPE YYKWTQWIFT KLYEKGLAYE AEVAVNWVPE LGTVISNEEV IDGKSERGGY 

       190        200        210        220        230        240 
DVVRRPMRQW MLKITAYADR LLEDLELVDW PESIKDMQRN WIGRSEGANV TFKVAGTEES 

       250        260        270        280        290        300 
FTVFTTRPDT LFGATYTVLA PELELVKKIT TPEQTAAVEA YIEETSKKSD LNRTDLAKEK 

       310        320        330        340        350        360 
TGVFTGAYAI NPVNGQEIPI WIGDYVLASY GTGAIMAVPA HDERDYEFAK TFGIDILPVI 

       370        380        390        400        410        420 
AGGDITTEAY TGDGPHINSD FLNGLNKAEA IAKMNEWLEE NHVGKKEVSY RLRDWLFSRQ 

       430        440        450        460        470        480 
RYWGEPIPVI HWEDGTTTTV PESELPLRLP VTSDIRPSGT GESPLANIDE WVNVVDPETG 

       490        500        510        520        530        540 
MKGKRETNTM PQWAGSSWYY LRFIDPHNKN EIADFEKLKR WLPVDIYIGG AEHAVLHLLY 

       550        560        570        580        590        600 
ARFWHKFLYD IGVVPTKEPF QKLYNQGMIL GENNEKMSKS RGNVVNPDDV VAKYGADTLR 

       610        620        630        640        650        660 
LYEMFMGPLD ASIAWNENGL EGSRKFLDRV WRLIVDEEGK MRDRITTIND GRLTKVYHQT 

       670        680        690        700        710        720 
VKKVTEDMAN LHFNTAISQL MVFVNEANKV DALPYEYVEG FVQLLAPIAP HIGEELWQIL 

       730        740        750        760        770        780 
GNEESLTYVP WPTYDETALV EDEVEVVFQV NGKLRGKQNV ARGLSKEELE QIAMNHEAVK 

       790        800 
EFIEGKTVRK VIAVPDKLVN IVAN 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE016830 Genomic DNA. Translation: AAO80614.1.
RefSeqNP_814544.1. NC_004668.1.

3D structure databases

ProteinModelPortalQ837N5.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING226185.EF0801.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAO80614; AAO80614; EF_0801.
GeneID1199694.
KEGGefa:EF0801.
PATRIC21851984. VBIEntFae7065_0741.

Phylogenomic databases

eggNOGCOG0495.
KOK01869.
OMAGIEHACM.
OrthoDBEOG63Z74X.
ProtClustDBPRK00390.

Enzyme and pathway databases

BioCycEFAE226185:GHI1-782-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_ENTFA
AccessionPrimary (citable) accession number: Q837N5
Entry history
Integrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: June 1, 2003
Last modified: April 16, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries