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Q837J0

- Q837J0_ENTFA

UniProt

Q837J0 - Q837J0_ENTFA

Protein

Alanine racemase

Gene

alr

Organism
Enterococcus faecalis (strain ATCC 700802 / V583)
Status
Unreviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 93 (01 Oct 2014)
      Sequence version 1 (01 Jun 2003)
      Previous versions | rss
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    • Comment

    Functioni

    Catalyzes the interconversion of L-alanine and D-alanine. May also act on other amino acids.UniRule annotation

    Catalytic activityi

    L-alanine = D-alanine.UniRule annotationSAAS annotation

    Cofactori

    Pyridoxal phosphate.UniRule annotationSAAS annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei40 – 401Proton acceptor; specific for D-alanineUniRule annotation
    Binding sitei40 – 401N6-(pyridoxalphosphate)lysine (covalent)Imported
    Binding sitei139 – 1391SubstrateUniRule annotation
    Active sitei267 – 2671Proton acceptor; specific for L-alanineUniRule annotation
    Binding sitei314 – 3141Substrate; via amide nitrogenUniRule annotation

    GO - Molecular functioni

    1. alanine racemase activity Source: UniProtKB-HAMAP
    2. pyridoxal phosphate binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. D-alanine biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    IsomeraseUniRule annotationSAAS annotation

    Keywords - Ligandi

    Pyridoxal phosphateUniRule annotationSAAS annotation

    Enzyme and pathway databases

    BioCyciEFAE226185:GHI1-830-MONOMER.
    UniPathwayiUPA00042; UER00497.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Alanine racemaseUniRule annotation (EC:5.1.1.1UniRule annotation)
    Gene namesi
    Name:alrImported
    Ordered Locus Names:EF_0849Imported
    ORF Names:I574_01250Imported
    OrganismiEnterococcus faecalis (strain ATCC 700802 / V583)Imported
    Taxonomic identifieri226185 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesEnterococcaceaeEnterococcus
    ProteomesiUP000001415: Chromosome

    PTM / Processingi

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei40 – 401N6-(pyridoxal phosphate)lysineUniRule annotation

    Interactioni

    Protein-protein interaction databases

    STRINGi226185.EF0849.

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3E5PX-ray2.50A/B/C1-371[»]
    3E6EX-ray2.50A/B/C1-371[»]
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ837J0.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the alanine racemase family.UniRule annotation

    Phylogenomic databases

    KOiK01775.
    OMAiVSTYDVG.
    OrthoDBiEOG6PP9NJ.

    Family and domain databases

    Gene3Di2.40.37.10. 1 hit.
    3.20.20.10. 1 hit.
    HAMAPiMF_01201. Ala_racemase.
    InterProiIPR000821. Ala_racemase.
    IPR009006. Ala_racemase/Decarboxylase_C.
    IPR011079. Ala_racemase_C.
    IPR001608. Ala_racemase_N.
    IPR020622. Ala_racemase_pyridoxalP-BS.
    IPR029066. PLP-binding_barrel.
    [Graphical view]
    PfamiPF00842. Ala_racemase_C. 1 hit.
    PF01168. Ala_racemase_N. 1 hit.
    [Graphical view]
    PRINTSiPR00992. ALARACEMASE.
    SMARTiSM01005. Ala_racemase_C. 1 hit.
    [Graphical view]
    SUPFAMiSSF50621. SSF50621. 1 hit.
    SSF51419. SSF51419. 1 hit.
    TIGRFAMsiTIGR00492. alr. 1 hit.
    PROSITEiPS00395. ALANINE_RACEMASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q837J0-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVVGWHRPTR LHIDTQAITE NVQKECQRLP EGTALFAVVK ANGYGHGAVE    50
    SAKAAKKGGA TGFCVALLDE AIELREAGVQ DPILILSVVD LAYVPLLIQY 100
    DLSVTVATQE WLEAALQQLT PESNTPLRVH LKVDTGMGRI GFLTPEETKQ 150
    AVRFVQSHKE FLWEGIFTHF STADEIDTSY FEKQAGRFKA VLAVLEELPR 200
    YVHVSNSATA LWHPDVPGNM IRYGVAMYGL NPSGNKLAPS YALKPALRLT 250
    SELIHVKRLA AGEGIGYGET YVTEAEEWIG TVPIGYADGW LRHLQGFTVL 300
    VNGKRCEIVG RVCMDQCMIR LAEEVPVGSV VTLVGKDGNE ENTLQMVAEK 350
    LETIHYEVAC TFSQRIPREY N 371
    Length:371
    Mass (Da):40,980
    Last modified:June 1, 2003 - v1
    Checksum:i4DE356FE62478E0B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE016830 Genomic DNA. Translation: AAO80661.1.
    ASWP01000005 Genomic DNA. Translation: EOT88132.1.
    RefSeqiNP_814591.1. NC_004668.1.

    Genome annotation databases

    EnsemblBacteriaiAAO80661; AAO80661; EF_0849.
    EOT88132; EOT88132; I574_01250.
    GeneIDi1199742.
    KEGGiefa:EF0849.
    PATRICi21852074. VBIEntFae7065_0786.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE016830 Genomic DNA. Translation: AAO80661.1 .
    ASWP01000005 Genomic DNA. Translation: EOT88132.1 .
    RefSeqi NP_814591.1. NC_004668.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3E5P X-ray 2.50 A/B/C 1-371 [» ]
    3E6E X-ray 2.50 A/B/C 1-371 [» ]
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 226185.EF0849.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAO80661 ; AAO80661 ; EF_0849 .
    EOT88132 ; EOT88132 ; I574_01250 .
    GeneIDi 1199742.
    KEGGi efa:EF0849.
    PATRICi 21852074. VBIEntFae7065_0786.

    Phylogenomic databases

    KOi K01775.
    OMAi VSTYDVG.
    OrthoDBi EOG6PP9NJ.

    Enzyme and pathway databases

    UniPathwayi UPA00042 ; UER00497 .
    BioCyci EFAE226185:GHI1-830-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei Q837J0.

    Family and domain databases

    Gene3Di 2.40.37.10. 1 hit.
    3.20.20.10. 1 hit.
    HAMAPi MF_01201. Ala_racemase.
    InterProi IPR000821. Ala_racemase.
    IPR009006. Ala_racemase/Decarboxylase_C.
    IPR011079. Ala_racemase_C.
    IPR001608. Ala_racemase_N.
    IPR020622. Ala_racemase_pyridoxalP-BS.
    IPR029066. PLP-binding_barrel.
    [Graphical view ]
    Pfami PF00842. Ala_racemase_C. 1 hit.
    PF01168. Ala_racemase_N. 1 hit.
    [Graphical view ]
    PRINTSi PR00992. ALARACEMASE.
    SMARTi SM01005. Ala_racemase_C. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50621. SSF50621. 1 hit.
    SSF51419. SSF51419. 1 hit.
    TIGRFAMsi TIGR00492. alr. 1 hit.
    PROSITEi PS00395. ALANINE_RACEMASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 700802 / V583Imported and V583Imported.
    2. "Structural insights into the alanine racemase from Enterococcus faecalis."
      Priyadarshi A., Lee E.H., Sung M.W., Nam K.H., Lee W.H., Kim E.E., Hwang K.Y.
      Biochim. Biophys. Acta 1794:1030-1040(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.50 ANGSTROMS) IN COMPLEX WITH N6-(PYRIDOXALPHOSPHATE)LYSINE.
    3. Cited for: NUCLEOTIDE SEQUENCE.
      Strain: V583Imported.

    Entry informationi

    Entry nameiQ837J0_ENTFA
    AccessioniPrimary (citable) accession number: Q837J0
    Entry historyi
    Integrated into UniProtKB/TrEMBL: June 1, 2003
    Last sequence update: June 1, 2003
    Last modified: October 1, 2014
    This is version 93 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    3D-structureImported, Complete proteome, Reference proteomeImported

    External Data

    Dasty 3