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Q836V1

- SYI_ENTFA

UniProt

Q836V1 - SYI_ENTFA

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Protein
Isoleucine--tRNA ligase
Gene
ileS, EF_1003
Organism
Enterococcus faecalis (strain ATCC 700802 / V583)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei552 – 5521Aminoacyl-adenylate By similarity
Binding sitei596 – 5961ATP By similarity
Metal bindingi886 – 8861Zinc By similarity
Metal bindingi889 – 8891Zinc By similarity
Metal bindingi906 – 9061Zinc By similarity
Metal bindingi909 – 9091Zinc By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciEFAE226185:GHI1-979-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:EF_1003
OrganismiEnterococcus faecalis (strain ATCC 700802 / V583)
Taxonomic identifieri226185 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesEnterococcaceaeEnterococcus
ProteomesiUP000001415: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 926926Isoleucine--tRNA ligaseUniRule annotation
PRO_0000098382Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi226185.EF1003.

Structurei

3D structure databases

ProteinModelPortaliQ836V1.
SMRiQ836V1. Positions 1-914.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi57 – 6711"HIGH" regionUniRule annotation
Add
BLAST
Motifi593 – 5975"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
KOiK01870.
OMAiLGRRSCQ.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q836V1-1 [UniParc]FASTAAdd to Basket

« Hide

MKMKETLQLG KTAFPMRGNL PNREAEWQKD WEEKGLYEQR QKLNEGKPTF    50
VLHDGPPYAN GNIHLGHSLN KISKDIIIRS KSMSGFRSPY VPGWDTHGLP 100
IEQVLTNKGV KRKEMTVAEY REKCKEYALS QVDKQRNDFK RLGVSGDWEH 150
PYITLDPEYE AAEIRVFGKM AEKGYIYKGL KPIYWSPSSE SSLAEAEIEY 200
KDVKSPSIYV AFNVADGKGL LDNETAFVIW TTTPWTLPAN LGISVNPDFT 250
YVEVKADGRK FVIAKDLLTT VKEAIGWEEV EVLREFSGEK LDRMTAQHPF 300
YDRTSLVMLG DHVTLDAGTG LVHTAPGHGE DDYIVSRKYD LPVISPVDSR 350
GVFTDEAPGF EGIFYDKANP MITELLEEKG ALLKLDFFTH SYPHDWRTKK 400
PVIYRATPQW FASISKFRQD ILDEVEKVDW LIPWGKTRLY NMIRDRGDWV 450
ISRQRAWGVP LPIFYAENGE AIITPETIEH VANLFAEHGS NIWFMREAKE 500
LLPAGFTHPG SPNGEFTKET DIMDVWFDSG SSHEGVLRER EELTFPADMY 550
LEGSDQYRGW FNSSITTSVA INGVAPYKSI ISQGMVLDGE GRKMSKSLGN 600
TILPEKVINQ MGADILRLWV SSVDAEADVR VSMDILNQVS EVYRKIRNTM 650
RFLLANTSDF NPAEHTVAYA DLRSVDKYMT VRLNQVIQEI RENGYEKYNF 700
MHIYRTVMNF LTVDLSSFYL DFAKDVVYIE AENDYQRRCM QTVFYQTLVS 750
LTKLLTPIIP HTAEEIWSFL QEEEEYVQLA EFPGYETFTN EEELMDTWAA 800
FMDFRDNVLK ALEEARHSKL IGKSLEAKVT VYPNEQIRQL MTAVDADIAQ 850
LLIVSDFEVS KEVAPSEAVQ FEDMAILVEK AEGETCDRCR SVRQDVGSDE 900
KLPTLCGRCA HIVEENYPEA VAEGFE 926
Length:926
Mass (Da):105,855
Last modified:June 1, 2003 - v1
Checksum:i64A81CBA01E2DE6E
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE016830 Genomic DNA. Translation: AAO80809.1.
RefSeqiNP_814739.1. NC_004668.1.

Genome annotation databases

EnsemblBacteriaiAAO80809; AAO80809; EF_1003.
GeneIDi1199891.
KEGGiefa:EF1003.
PATRICi21852376. VBIEntFae7065_0936.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE016830 Genomic DNA. Translation: AAO80809.1 .
RefSeqi NP_814739.1. NC_004668.1.

3D structure databases

ProteinModelPortali Q836V1.
SMRi Q836V1. Positions 1-914.
ModBasei Search...

Protein-protein interaction databases

STRINGi 226185.EF1003.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAO80809 ; AAO80809 ; EF_1003 .
GeneIDi 1199891.
KEGGi efa:EF1003.
PATRICi 21852376. VBIEntFae7065_0936.

Phylogenomic databases

eggNOGi COG0060.
KOi K01870.
OMAi LGRRSCQ.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci EFAE226185:GHI1-979-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700802 / V583.

Entry informationi

Entry nameiSYI_ENTFA
AccessioniPrimary (citable) accession number: Q836V1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: June 1, 2003
Last modified: May 14, 2014
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi