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Q836R3 (PFKA_ENTFA) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 77. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
ATP-dependent 6-phosphofructokinase

Short name=ATP-PFK
Short name=Phosphofructokinase
EC=2.7.1.11
Alternative name(s):
Phosphohexokinase
Gene names
Name:pfkA
Ordered Locus Names:EF_1045
OrganismEnterococcus faecalis (strain ATCC 700802 / V583) [Reference proteome] [HAMAP]
Taxonomic identifier226185 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesEnterococcaceaeEnterococcus

Protein attributes

Sequence length320 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis By similarity. HAMAP-Rule MF_00339

Catalytic activity

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate. HAMAP-Rule MF_00339

Cofactor

Magnesium By similarity. HAMAP-Rule MF_00339

Enzyme regulation

Allosterically activated by ADP and other diphosphonucleosides, and allosterically inhibited by phosphoenolpyruvate By similarity. HAMAP-Rule MF_00339

Pathway

Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4. HAMAP-Rule MF_00339

Subunit structure

Homotetramer By similarity. HAMAP-Rule MF_00339

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00339.

Sequence similarities

Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Prokaryotic clade "B1" sub-subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 320320ATP-dependent 6-phosphofructokinase HAMAP-Rule MF_00339
PRO_1000059761

Regions

Nucleotide binding72 – 732ATP By similarity
Nucleotide binding102 – 1054ATP By similarity
Region21 – 255Allosteric activator ADP binding; shared with dimeric partner By similarity
Region125 – 1273Substrate binding By similarity
Region169 – 1713Substrate binding By similarity
Region185 – 1873Allosteric activator ADP binding By similarity
Region213 – 2153Allosteric activator ADP binding By similarity
Region249 – 2524Substrate binding By similarity

Sites

Active site1271Proton acceptor By similarity
Metal binding1031Magnesium; catalytic By similarity
Binding site111ATP; via amide nitrogen By similarity
Binding site1541Allosteric activator ADP By similarity
Binding site1621Substrate; shared with dimeric partner By similarity
Binding site2111Allosteric activator ADP By similarity
Binding site2221Substrate By similarity
Binding site2431Substrate; shared with dimeric partner By similarity

Sequences

Sequence LengthMass (Da)Tools
Q836R3 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: 023FF5C80FFAA966

FASTA32034,412
        10         20         30         40         50         60 
MKRIGILTSG GDAPGMNAAI RAVVRKSIFD GIEVYGINYG FAGLVAGDIR RLDVADVGDK 

        70         80         90        100        110        120 
IQRGGTFLYS ARYPEFATEE GQLKGIEQLK KFGIEGLVVI GGDGSYHGAM ALTKRGFPAV 

       130        140        150        160        170        180 
GIPGTIDNDI PGTDFTIGFD TAINTVLESI DRIRDTATSH VRTFVIEVMG RNAGDIALWS 

       190        200        210        220        230        240 
GVAGGADEII IPEHDFDMKN VAKRIQEGRD RGKKHCLIIL AEGVMGGNEF ADKLSEYGDF 

       250        260        270        280        290        300 
HTRVSILGHV VRGGSPSARD RVLASKFGSY AVELLKEGKG GLCIGMLDNQ VVAADIIDTL 

       310        320 
ENNKHKPDLS LYELNHEISF 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE016830 Genomic DNA. Translation: AAO80848.1.
RefSeqNP_814778.1. NC_004668.1.

3D structure databases

ProteinModelPortalQ836R3.
SMRQ836R3. Positions 1-319.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING226185.EF1045.

Proteomic databases

PRIDEQ836R3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAO80848; AAO80848; EF_1045.
GeneID1199933.
KEGGefa:EF1045.
PATRIC21852462. VBIEntFae7065_0978.

Phylogenomic databases

eggNOGCOG0205.
KOK00850.
OMAAKLERGH.
OrthoDBEOG644ZRM.

Enzyme and pathway databases

BioCycEFAE226185:GHI1-1021-MONOMER.
UniPathwayUPA00109; UER00182.

Family and domain databases

HAMAPMF_00339. Phosphofructokinase_I_B1.
InterProIPR012003. ATP_PFK_prok.
IPR012828. PFKA_ATP.
IPR022953. Phosphofructokinase.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamPF00365. PFK. 1 hit.
[Graphical view]
PIRSFPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSPR00476. PHFRCTKINASE.
SUPFAMSSF53784. SSF53784. 1 hit.
TIGRFAMsTIGR02482. PFKA_ATP. 1 hit.
ProtoNetSearch...

Entry information

Entry namePFKA_ENTFA
AccessionPrimary (citable) accession number: Q836R3
Entry history
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: June 1, 2003
Last modified: July 9, 2014
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways