Q836J0 (MURI_ENTFA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate racemase
EC=5.1.1.3

Gene names

Name:	murI
Ordered Locus Names:	EF_1121

Organism

Enterococcus faecalis (Streptococcus faecalis)

Taxonomic identifier

1351 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Enterococcaceae › Enterococcus

Protein attributes

Sequence length	273 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Provides the (R)-glutamate required for cell wall biosynthesis By similarity. HAMAP MF_00258
Catalytic activity	L-glutamate = D-glutamate. HAMAP MF_00258
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00258
Sequence similarities	Belongs to the aspartate/glutamate racemases family.

Ontologies

Keywords
Biological process	Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Molecular function	Isomerase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	glutamate racemase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 273

273

Glutamate racemase HAMAP MF_00258

PRO_1000047564

Secondary structure

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273

Helix Strand Turn

Details...

Beta strand

7 – 14

8

Helix

17 – 26

10

Beta strand

32 – 36

5

Helix

38 – 40

3

Helix

48 – 63

16

Turn

64 – 66

3

Beta strand

68 – 72

5

Helix

75 – 88

14

Beta strand

93 – 96

4

Helix

97 – 107

11

Beta strand

109 – 117

9

Helix

119 – 124

6

Helix

126 – 132

7

Beta strand

138 – 144

7

Helix

148 – 153

6

Helix

160 – 169

10

Helix

170 – 173

4

Turn

174 – 176

3

Beta strand

179 – 183

5

Helix

188 – 191

4

Helix

192 – 199

8

Beta strand

203 – 207

5

Helix

208 – 222

15

Beta strand

237 – 242

6

Helix

244 – 255

12

Sequences

	Sequence		Length	Mass (Da)
	Q836J0 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 1F2ADF4D0B93991D	FASTA	273	29,479
	10 20 30 40 50 60 MSNQEAIGLI DSGVGGLTVL KEALKQLPNE RLIYLGDTAR CPYGPRPAEQ VVQFTWEMAD 70 80 90 100 110 120 FLLKKRIKML VIACNTATAV ALEEIKAALP IPVVGVILPG ARAAVKVTKN NKIGVIGTLG 130 140 150 160 170 180 TIKSASYEIA IKSKAPTIEV TSLDCPKFVP IVESNQYRSS VAKKIVAETL QALQLKGLDT 190 200 210 220 230 240 LILGCTHYPL LRPVIQNVMG SHVTLIDSGA ETVGEVSMLL DYFDIAHTPE APTQPHEFYT 250 260 270 TGSAKMFEEI ASSWLGIENL KAQQIHLGGN END

References

[1]

"Role of mobile DNA in the evolution of vancomycin-resistant Enterococcus faecalis." Paulsen I.T., Banerjei L., Myers G.S.A., Nelson K.E., Seshadri R., Read T.D., Fouts D.E., Eisen J.A., Gill S.R., Heidelberg J.F., Tettelin H., Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J., Daugherty S.C., DeBoy R.T., Durkin S.A. , Kolonay J.F., Madupu R., Nelson W.C., Vamathevan J.J., Tran B., Upton J., Hansen T., Shetty J., Khouri H.M., Utterback T.R., Radune D., Ketchum K.A., Dougherty B.A., Fraser C.M. Science 299:2071-2074(2003) [PubMed: 12663927] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700802 / V583.

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Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AE016830 Genomic DNA. Translation: AAO80921.1.

RefSeq

NP_814851.1. NC_004668.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2JFO	X-ray	2.50	A/B	1-273	[»]
2JFP	X-ray	1.98	A/B	1-273	[»]
2VVT	X-ray	1.65	A/B	1-270	[»]

ProteinModelPortal

Q836J0.

SMR

Q836J0. Positions 2-268.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

1200023.

GenomeReviews

Gene locus EF_1121 in contig AE016830_GR.

KEGG

efa:EF1121.

NMPDR

fig|226185.1.peg.1036.

PATRIC

21852632. VBIEntFae7065_1047.

TIGR

EF_1121.

Phylogenomic databases

HOGENOM

HBG645102.

OMA

KVEFHAS.

ProtClustDB

PRK00865.

Enzyme and pathway databases

BioCyc

EFAE226185:EF_1121-MONOMER.

Family and domain databases

HAMAP

MF_00258. Glu_racemase.
[Tree]

InterPro

IPR015942. Asp/Glu/hydantoin_racemase.
IPR001920. Asp/Glu_race.
IPR018187. Asp/Glu_racemase_AS.
IPR004391. Glu_race.
[Graphical view]

Gene3D

G3DSA:3.40.50.1860. Asp/Glu_race. 1 hit.

KO

K01776.

PANTHER

PTHR21198. PTHR21198. 1 hit.

Pfam

PF01177. Asp_Glu_race. 1 hit.
[Graphical view]

SUPFAM

SSF53681. Asp/Glu_race. 2 hits.

TIGRFAMs

TIGR00067. Glut_race. 1 hit.

PROSITE

PS00923. ASP_GLU_RACEMASE_1. 1 hit.
PS00924. ASP_GLU_RACEMASE_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

MURI_ENTFA

Accession

Primary (citable) accession number: Q836J0

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	June 1, 2003
Last modified:	January 25, 2012
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program