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Q834R3 (TYSY_ENTFA) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 69. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Thymidylate synthase
Short name=TS
Short name=TSase
EC=2.1.1.45
Gene names
Name:thyA
Ordered Locus Names:EF_1576
OrganismEnterococcus faecalis (strain ATCC 700802 / V583) [Reference proteome] [HAMAP]
Taxonomic identifier226185 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacilli › Lactobacillales › Enterococcaceae › Enterococcus ›

Protein attributes

Sequence length315 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Provides the sole de novo source of dTMP for DNA biosynthesis By similarity. HAMAP-Rule MF_00008

Catalytic activity

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP. HAMAP-Rule MF_00008

Pathway

Pyrimidine metabolism; dTTP biosynthesis. HAMAP-Rule MF_00008

Subunit structure

Homodimer By similarity.

Subcellular location

Cytoplasm By similarity.

Sequence similarities

Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.

Ontologies

Keywords
   Biological processNucleotide biosynthesis
   Cellular componentCytoplasm
   Molecular functionMethyltransferase
Transferase
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processdTMP biosynthetic process

Inferred from electronic annotation. Source: HAMAP

dTTP biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionthymidylate synthase activity

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 315315Thymidylate synthase HAMAP-Rule MF_00008
PRO_0000140957

Sites

Active site1971 By similarity

Secondary structure

1 ............................................... 315
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q834R3 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: 0593A11D77D2D0A2

FASTA31536,344
        10         20         30         40         50         60 
MEEAYLALGK KILEEGHFKE DRTGTGTYSL FGYQMRFDLA KGFPLLTTKR VPFGLIKSEL 

        70         80         90        100        110        120 
LWFLKGDTNI RYLLERNNHI WDEWAFERYV KSADYQGPDM TDFGHRVLQD PAFAEQYKEE 

       130        140        150        160        170        180 
HQKFCDAILN DAEFAEKYGE LGNIYGAQWR HWETKDGSFI DQLANVIEMI KTNPDSRRLI 

       190        200        210        220        230        240 
VSAWNPEDVP SMALPPCHTM FQFYVNEGKL SCQLYQRSAD VFLGVPFNIA SYALLTHLIA 

       250        260        270        280        290        300 
HETGLEVGEF VHTLGDAHLY QNHVEQMQEQ LSREVRSFPT LVLNPDKASV FDFDMEDIKV 

       310 
EGYDPHPTIK APIAV

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE016830 Genomic DNA. Translation: AAO81363.1.
RefSeqNP_815293.1. NC_004668.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3UWLX-ray2.07A/B/C/D1-315[»]
ProteinModelPortalQ834R3.
SMRQ834R3. Positions 1-315.
ModBaseSearch...

Protein-protein interaction databases

STRING226185.EF1576.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAO81363; AAO81363; EF_1576.
GeneID1200476.
KEGGefa:EF1576.
PATRIC21853508. VBIEntFae7065_1480.

Phylogenomic databases

eggNOGCOG0207.
KOK00560.
OMADTNVAYL.
ProtClustDBPRK01827.

Enzyme and pathway databases

BioCycEFAE226185:GHI1-1661-MONOMER.
UniPathwayUPA00575.

Family and domain databases

Gene3D3.30.572.10. 2 hits.
HAMAPMF_00008. Thymidy_synth_bact.
InterProIPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
IPR020940. Thymidylate_synthase_AS.
[Graphical view]
PfamPF00303. Thymidylat_synt. 1 hit.
[Graphical view]
PRINTSPR00108. THYMDSNTHASE.
SUPFAMSSF55831. Thymidylat_synth_C. 1 hit.
TIGRFAMsTIGR03284. thym_sym. 1 hit.
PROSITEPS00091. THYMIDYLATE_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

BindingDBQ834R3.
ChEMBLCHEMBL1795144.

Entry information

Entry nameTYSY_ENTFA
AccessionPrimary (citable) accession number: Q834R3
Entry history
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: June 1, 2003
Last modified: May 1, 2013
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

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