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Protein

Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFO

Gene

trmFO

Organism
Enterococcus faecalis (strain ATCC 700802 / V583)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the folate-dependent formation of 5-methyl-uridine at position 54 (M-5-U54) in all tRNAs.UniRule annotation

Catalytic activityi

5,10-methylenetetrahydrofolate + uracil(54) in tRNA + FADH2 = tetrahydrofolate + 5-methyluracil(54) in tRNA + FAD.UniRule annotation

Cofactori

FADUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi9 – 146FADUniRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

FAD, Flavoprotein

Enzyme and pathway databases

BioCyciEFAE226185:GHI1-1629-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFOUniRule annotation (EC:2.1.1.74UniRule annotation)
Alternative name(s):
Folate-dependent tRNA (uracil-5-)-methyltransferaseUniRule annotation
Folate-dependent tRNA(M-5-U54)-methyltransferaseUniRule annotation
Gene namesi
Name:trmFOUniRule annotation
Synonyms:gid
Ordered Locus Names:EF_1649
OrganismiEnterococcus faecalis (strain ATCC 700802 / V583)
Taxonomic identifieri226185 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesEnterococcaceaeEnterococcus
Proteomesi
  • UP000001415 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 435435Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFOPRO_0000117243Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi226185.EF1649.

Structurei

3D structure databases

ProteinModelPortaliQ834K1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the MnmG family. TrmFO subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107QXI. Bacteria.
COG1206. LUCA.
KOiK04094.
OMAiDYLNCPM.
OrthoDBiEOG6J74VT.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.50.50.60. 2 hits.
HAMAPiMF_01037. TrmFO.
InterProiIPR023753. FAD/NAD-binding_dom.
IPR002218. MnmG-rel.
IPR020595. MnmG-rel_CS.
IPR016040. NAD(P)-bd_dom.
IPR004417. TrmFO.
[Graphical view]
PfamiPF01134. GIDA. 1 hit.
[Graphical view]
SUPFAMiSSF51905. SSF51905. 1 hit.
TIGRFAMsiTIGR00137. gid_trmFO. 1 hit.
PROSITEiPS01281. GIDA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q834K1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTKKVTIIGA GLAGSEAAWQ VANAGVPVDL YEMRPVKKTP AHQTENFAEL
60 70 80 90 100
VCSNSLRGNS LTNAVGVLKE EMRRLNSIII GSADQTAVPA GGALAVDRDS
110 120 130 140 150
FSETITEKIK SHPLITIKNE EITDIPEGIV IIATGPLTSE SLSQKIQEFN
160 170 180 190 200
GSEGFYFYDA AAPIIDKSTI DMDKVYLKSR YNKGEAAYLN CPMTEEEFNA
210 220 230 240 250
FHEALVNAEV VPLRTFEKEK FFEGCMPIEV MAQRGIKTML FGPMKPVGLE
260 270 280 290 300
DPKTGKRPYA VIQLRQDNAA ASLYNIVGFQ THLKWGEQKR VFRMIPGLEN
310 320 330 340 350
AEFVRYGVMH RNSFMNSPEL LKPTYQSKKR DDLFFAGQMT GVEGYVESAA
360 370 380 390 400
SGLLAGINAA RLAKGEEPIE FPRETTLGSM AYYITHAEGK HFQPMNANFG
410 420 430
LFPELPERIR DKKERYEAIA NRALDVQAQV IQSLD
Length:435
Mass (Da):48,248
Last modified:June 1, 2003 - v1
Checksum:iE4A30EE7B9D5B198
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016830 Genomic DNA. Translation: AAO81427.1.
RefSeqiNP_815357.1. NC_004668.1.
WP_002357486.1. NZ_KE136528.1.

Genome annotation databases

EnsemblBacteriaiAAO81427; AAO81427; EF_1649.
GeneIDi1200543.
KEGGiefa:EF1649.
PATRICi21853644. VBIEntFae7065_1548.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016830 Genomic DNA. Translation: AAO81427.1.
RefSeqiNP_815357.1. NC_004668.1.
WP_002357486.1. NZ_KE136528.1.

3D structure databases

ProteinModelPortaliQ834K1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi226185.EF1649.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAO81427; AAO81427; EF_1649.
GeneIDi1200543.
KEGGiefa:EF1649.
PATRICi21853644. VBIEntFae7065_1548.

Phylogenomic databases

eggNOGiENOG4107QXI. Bacteria.
COG1206. LUCA.
KOiK04094.
OMAiDYLNCPM.
OrthoDBiEOG6J74VT.

Enzyme and pathway databases

BioCyciEFAE226185:GHI1-1629-MONOMER.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.50.50.60. 2 hits.
HAMAPiMF_01037. TrmFO.
InterProiIPR023753. FAD/NAD-binding_dom.
IPR002218. MnmG-rel.
IPR020595. MnmG-rel_CS.
IPR016040. NAD(P)-bd_dom.
IPR004417. TrmFO.
[Graphical view]
PfamiPF01134. GIDA. 1 hit.
[Graphical view]
SUPFAMiSSF51905. SSF51905. 1 hit.
TIGRFAMsiTIGR00137. gid_trmFO. 1 hit.
PROSITEiPS01281. GIDA_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700802 / V583.

Entry informationi

Entry nameiTRMFO_ENTFA
AccessioniPrimary (citable) accession number: Q834K1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 25, 2003
Last sequence update: June 1, 2003
Last modified: January 20, 2016
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.