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Q83444 (NSP1_ROTME) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 43. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Non-structural protein 1

Short name=NSP1
Alternative name(s):
NCVP2
Non-structural RNA-binding protein 53
Short name=NS53
OrganismRotavirus A (isolate Mouse/Brazil/EHP/1981 G16-Px[20]-Ix-Rx-Cx-Mx-A7-Nx-Tx-E7-Hx) (RV-A)
Taxonomic identifier578840 [NCBI]
Taxonomic lineageVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirusRotavirus A
Virus hostMus musculus musculus (eastern European house mouse) [TaxID: 39442]

Protein attributes

Sequence length493 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Seems to induce the proteasome-dependent degradation of IRF3, IRF5 and IRF7, thereby antagonizing the cellular interferon response and establishment of the antiviral state. Binds and targets IRF3 early post-infection and suppresses IRF3 nuclear translocation By similarity.

Subunit structure

Interacts (via C-terminus) with host IRF3; this interaction leads to IRF3 degradation. Interacts with host IRF7; this interaction leads to IRF7 degradation By similarity.

Subcellular location

Host cytoplasmhost cytoskeleton By similarity.

Domain

The zinc-finger domain is important, but not sufficient for binding and degrading IRF3. It is sometimes described as a RING zinc-finger, but it is atypical and it is unclear whether it is related with ubiquitin ligase activity By similarity.

Sequence similarities

Belongs to the rotavirus A NSP1 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 493493Non-structural protein 1
PRO_0000369078

Regions

Zinc finger42 – 7938 Potential
Region1 – 8181RNA-binding By similarity
Region82 – 17695Important for cytoskeleton localization By similarity
Region315 – 493179Interaction with IRF3 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q83444 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: DEE472048A58EBA7

FASTA49357,597
        10         20         30         40         50         60 
MAAFKDACFH YRRITKLNRE LLRIGANSSW TPAPPSNIRG WCLECCQLTN LTYCYGCSLH 

        70         80         90        100        110        120 
HVCQWCVQYG RCFLDDEPHL LRLRTVKSPI TTEKLASIVK MYQLLFPINH SIVKKFVKST 

       130        140        150        160        170        180 
KQHKCRNDFE LSWYNQLVLP ITLTAAAVHC DDCIYYIFGH YEGKANQSNL PYRFVNCVDE 

       190        200        210        220        230        240 
YDRLLLDDVN FDRMAFLPGR LQKYYAKRYF IASRIPSAQP AKLTYSDFSV KTLINSGAYA 

       250        260        270        280        290        300 
RRRIIYRSVT NFHWQSHEDP LNDLLLDKDK ILAALMTNER RPFLTHNLNF TSLLHELSEL 

       310        320        330        340        350        360 
VHHAKPCYLH SFHVQPASKV HCHSCSVAFD FHTVDWRIRR IYDDVMYFLR ACCRSNVSSG 

       370        380        390        400        410        420 
SCSSLDPMDA VVKAALLEMF TESFKHHAHL LFHCFDPVQI DDVSYILFNY PVNYDIYDFI 

       430        440        450        460        470        480 
IRTLATERLP FTLSYKQFTT ILFALVERWY DLSQIERLPL SIAPTNRLIE LQERGNLAEE 

       490 
FDLLLSSSDS EED 

« Hide

References

[1]"Comparison of the rotavirus nonstructural protein NSP1 (NS53) from different species by sequence analysis and northern blot hybridization."
Dunn S.J., Cross T.L., Greenberg H.B.
Virology 203:178-183(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U08423 Genomic RNA. Translation: AAA50486.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR002148. Rotavirus_NSP1.
[Graphical view]
PfamPF00981. Rota_NS53. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNSP1_ROTME
AccessionPrimary (citable) accession number: Q83444
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: November 1, 1996
Last modified: February 19, 2014
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families