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Q83440

- VP4_ROTMB

UniProt

Q83440 - VP4_ROTMB

Protein

Outer capsid protein VP4

Gene
N/A
Organism
Rotavirus A (isolate Mouse/United States/Eb/1982 G16-P10[16]-Ix-Rx-Cx-Mx-Ax-Nx-Tx-E7-Hx) (RV-A)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 73 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Spike-forming protein that mediates virion attachment to the host epithelial cell receptors and plays a major role in cell penetration, determination of host range restriction and virulence. Rotavirus entry into the host cell probably involves multiple sequential contacts between the outer capsid proteins VP4 and VP7, and the cell receptors. According to the considered strain, VP4 seems to essentially target sialic acid and/or the integrin heterodimer ITGA2/ITGB1 By similarity.By similarity
    Outer capsid protein VP5*: forms the spike "foot" and "body". Acts as a membrane permeabilization protein that mediates release of viral particles from endosomal compartments into the cytoplasm. In integrin-dependent strains, VP5* targets the integrin heterodimer ITGA2/ITGB1 for cell attachment By similarity.By similarity
    VP8* forms the head of the spikes. It is the viral hemagglutinin and an important target of neutralizing antibodies. In sialic acid-dependent strains, VP8* binds to host cell sialic acid, most probably a ganglioside, providing the initial contact By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei230 – 2312CleavageBy similarity
    Sitei246 – 2472CleavageBy similarity

    GO - Biological processi

    1. permeabilization of host organelle membrane involved in viral entry into host cell Source: UniProtKB-KW
    2. virion attachment to host cell Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hemagglutinin

    Keywords - Biological processi

    Host-virus interaction, Viral attachment to host cell, Viral penetration into host cytoplasm, Viral penetration via permeabilization of host membrane, Virus entry into host cell

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Outer capsid protein VP4
    Alternative name(s):
    Hemagglutinin
    Cleaved into the following 2 chains:
    OrganismiRotavirus A (isolate Mouse/United States/Eb/1982 G16-P10[16]-Ix-Rx-Cx-Mx-Ax-Nx-Tx-E7-Hx) (RV-A)
    Taxonomic identifieri578842 [NCBI]
    Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirusRotavirus A
    Virus hostiMus musculus musculus (eastern European house mouse) [TaxID: 39442]

    Subcellular locationi

    Chain Outer capsid protein VP4 : Virion By similarity. Host rough endoplasmic reticulum Curated
    Note: Immature double-layered particles assembled in the cytoplasm bud across the membrane of the endoplasmic reticulum, acquiring during this process a transient lipid membrane that is modified with the ER resident viral glycoproteins NSP4 and VP7; these enveloped particles also contain VP4. As the particles move towards the interior of the ER cisternae, the transient lipid membrane and the non-structural protein NSP4 are lost, while the virus surface proteins VP4 and VP7 rearrange to form the outermost virus protein layer, yielding mature infectious triple-layered particles By similarity.By similarity
    Chain Outer capsid protein VP8* : Virion
    Note: Outer capsid protein.By similarity
    Chain Outer capsid protein VP5* : Virion
    Note: Outer capsid protein.By similarity

    GO - Cellular componenti

    1. host cell rough endoplasmic reticulum Source: UniProtKB-SubCell
    2. viral outer capsid Source: UniProtKB-KW

    Keywords - Cellular componenti

    Capsid protein, Host endoplasmic reticulum, Outer capsid protein, Virion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 775775Outer capsid protein VP4PRO_0000368125Add
    BLAST
    Chaini1 – 230230Outer capsid protein VP8*PRO_0000368126Add
    BLAST
    Chaini247 – 775529Outer capsid protein VP5*PRO_0000368127Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi32 – 321N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi57 – 571N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi111 – 1111N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi149 – 1491N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi197 – 1971N-linked (GlcNAc...); by hostSequence Analysis
    Disulfide bondi317 ↔ 379Sequence Analysis
    Glycosylationi669 – 6691N-linked (GlcNAc...); by hostSequence Analysis

    Post-translational modificationi

    Proteolytic cleavage by trypsin results in activation of VP4 functions and greatly increases infectivity. The penetration into the host cell is dependent on trypsin treatment of VP4. It produces two peptides, VP5* and VP8* that remain associated with the virion By similarity.By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Interactioni

    Subunit structurei

    VP4 is a homotrimer Potential. VP4 adopts a dimeric appearance above the capsid surface, while forming a trimeric base anchored inside the capsid layer. Only hints of the third molecule are observed above the capsid surface. It probably performs a series of molecular rearrangements during viral entry. Prior to trypsin cleavage, it is flexible. The priming trypsin cleavage triggers its rearrangement into rigid spikes with approximate two-fold symmetry of their protruding parts. After an unknown second triggering event, cleaved VP4 may undergo another rearrangement, in which two VP5* subunits fold back on themselves and join a third subunit to form a tightly associated trimer, shaped like a folded umbrella. VP5* is a homotrimer Potential. The trimer is coiled-coil stabilized by its C-terminus, however, its N-terminus, known as antigen domain or "body", seems to be flexible allowing it to self-associate either as a dimer or a trimer. The two- to three-fold reorganization and fold-back of VP5* may be linked to membrane penetration, by exposing its hydrophobic region. Interacts with host ITGA2 (via ITAG2 I-domain); this interaction occurs when ITGA2 is part of the integrin heterodimer ITGA2/ITGB1. Interacts with host integrin heterodimer ITGA4/ITGB1 and ITGA4/ITGB7 By similarity.By similarityCurated

    Structurei

    3D structure databases

    ProteinModelPortaliQ83440.
    SMRiQ83440. Positions 65-223, 252-521.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni247 – 479233Antigen domainBy similarityAdd
    BLAST
    Regioni307 – 3093DGE motif; interaction with ITGA2/ITGB1 heterodimerBy similarity
    Regioni388 – 40821Hydrophobic; possible role in virus entry into host cellSequence AnalysisAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili483 – 51735Sequence AnalysisAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi559 – 61557Ser-richAdd
    BLAST

    Sequence similaritiesi

    Belongs to the rotavirus VP4 family.Curated

    Keywords - Domaini

    Coiled coil

    Family and domain databases

    Gene3Di2.60.120.200. 1 hit.
    InterProiIPR008985. ConA-like_lec_gl_sf.
    IPR013320. ConA-like_subgrp.
    IPR000416. Haemagglutinin_VP4.
    [Graphical view]
    PfamiPF00426. VP4_haemagglut. 1 hit.
    [Graphical view]
    SUPFAMiSSF49899. SSF49899. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q83440-1 [UniParc]FASTAAdd to Basket

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    MASLIYRQLL TNSFTVDLSD EIETIGAEKT KNVTVNPGPF AQTGYAPVNW    50
    GPGETSNSTT VEPVLDGPYQ PIAFSPPSEY YILLSPTVPG VVVECTNTVD 100
    RWIATIAIEP NVSKTKRTYT LFGITEQLTV ENSYADKWKF IDFLKASTNG 150
    SYARYNILLS STKLCAVAKH TDRLYSYIRE TPNAGQAYYA FSHNIFNLTA 200
    HCDFYIIPWA QQSLCTQYIN TGLPPIQNTR NVVARHLSAR SIITRRAQAN 250
    EDIVVSKTSL WKEMQFNRDI TIRFKFANAI IKSGGLGYKW SEISFKPANY 300
    QYTYTRDGEE VTAHTTCSVN GVNNFDFFGG SLPTDFGISR YEVLKENSFV 350
    YIDYWDDSQA FRNMVYVRSL AADLNTIECT GGTYEFSLPV GQWPAMTGGA 400
    VSLRVAGVTL STQYTDFVSL NSLRFRFRLS VEEPPFSITR TRVSGLYGLP 450
    AANPNNGKEY YELAGRFSLI SLVPSNDNYQ TPIMNSVTVR QDLERQLGEL 500
    REEFNALSQE IALSQLVDLA LLPLDMFSMF SGIKATLDVA KSMATNVMKK 550
    FKSSGLATSV SAMTESLSDA ASSVSRSNTI RSISSTSSAW TDVSSRVADL 600
    DNAASTVSTQ TATISRRLRL KEITTQTEGM NFDDISAAVL KIKLDKSAQI 650
    APNTLPDIVT EASEKFIPNR SYRIINNNEA FEAGTDGRFF AYRVDTLEEL 700
    PFDVQKFANL VTESPVISAI IDFKTLRNLN DNYGISKEQA FNLLRSDPRV 750
    LREFINQGNP IIRNRIEQLI MQCKL 775
    Length:775
    Mass (Da):86,493
    Last modified:November 1, 1996 - v1
    Checksum:i5162D98EB313BD54
    GO

    Sequence cautioni

    The sequence AAA47356.1 differs from that shown. Reason: Frameshift at position 88.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti46 – 461A → P in AAA47356. (PubMed:8122380)Curated
    Sequence conflicti218 – 2181Y → S in AAA47356. (PubMed:8122380)Curated
    Sequence conflicti234 – 2341A → S in AAA47356. (PubMed:8122380)Curated
    Sequence conflicti253 – 2542IV → RC in AAA47356. (PubMed:8122380)Curated
    Sequence conflicti269 – 2691D → N in AAA47356. (PubMed:8122380)Curated
    Sequence conflicti504 – 5041F → C in AAA47356. (PubMed:8122380)Curated
    Sequence conflicti537 – 5371L → F in AAA47356. (PubMed:8122380)Curated
    Sequence conflicti558 – 5581T → A in AAA47356. (PubMed:8122380)Curated
    Sequence conflicti615 – 6162SR → RQ in AAA47356. (PubMed:8122380)Curated
    Sequence conflicti666 – 6661F → L in AAA47356. (PubMed:8122380)Curated
    Sequence conflicti674 – 6752II → TT in AAA47356. (PubMed:8122380)Curated
    Sequence conflicti749 – 7491R → G in AAA47356. (PubMed:8122380)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U08419 Genomic RNA. Translation: AAA50482.1.
    L18992 Genomic DNA. Translation: AAA47356.1. Frameshift.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U08419 Genomic RNA. Translation: AAA50482.1 .
    L18992 Genomic DNA. Translation: AAA47356.1 . Frameshift.

    3D structure databases

    ProteinModelPortali Q83440.
    SMRi Q83440. Positions 65-223, 252-521.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 2.60.120.200. 1 hit.
    InterProi IPR008985. ConA-like_lec_gl_sf.
    IPR013320. ConA-like_subgrp.
    IPR000416. Haemagglutinin_VP4.
    [Graphical view ]
    Pfami PF00426. VP4_haemagglut. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49899. SSF49899. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Comparison of VP4 and VP7 of five murine rotavirus strains."
      Dunn S.J., Burns J.W., Cross T.L., Vo P.T., Ward R.L., Bremont M., Greenberg H.B.
      Virology 203:250-259(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    2. "The outer capsid protein VP4 of murine rotavirus strain Eb represents a tentative new P type."
      Sereno M.M., Gorziglia M.I.
      Virology 199:500-504(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

    Entry informationi

    Entry nameiVP4_ROTMB
    AccessioniPrimary (citable) accession number: Q83440
    Secondary accession number(s): Q86227
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 14, 2009
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 73 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    In group A rotaviruses, VP4 defines the P serotype.
    This strain has been shown to be sialic acid-independent in cell culture conditions.By similarity

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3