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Q83356

- HEMA_CVMJH

UniProt

Q83356 - HEMA_CVMJH

Protein

Hemagglutinin-esterase

Gene

HE

Organism
Murine coronavirus (strain JHM) (MHV-JHM) (Murine hepatitis virus)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 88 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Structural protein that makes short spikes at the surface of the virus. Contains receptor binding and receptor-destroying activities. Mediates de-O-acetylation of N-acetyl-4-O-acetylneuraminic acid, which is probably the receptor determinant recognized by the virus on the surface of erythrocytes and susceptible cells. This receptor-destroying activity is important for virus release as it probably helps preventing self-aggregation and ensures the efficient spread of the progeny virus from cell to cell. May serve as a secondary viral attachment protein for initiating infection, the spike protein being the major one. Seems to be a 'luxury' protein that is not absolutely necessary for virus infection in culture. However, its presence in the virus may alter its pathogenicity. May become a target for both the humoral and the cellular branches of the immune system.

    Catalytic activityi

    N-acetyl-O-acetylneuraminate + H2O = N-acetylneuraminate + acetate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei45 – 451NucleophileBy similarity
    Active sitei342 – 3421Charge relay systemBy similarity
    Active sitei345 – 3451Charge relay systemBy similarity

    GO - Molecular functioni

    1. sialate O-acetylesterase activity Source: UniProtKB-EC

    GO - Biological processi

    1. fusion of virus membrane with host plasma membrane Source: InterPro

    Keywords - Molecular functioni

    Hemagglutinin, Hydrolase

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Hemagglutinin-esterase (EC:3.1.1.53)
    Short name:
    HE protein
    Alternative name(s):
    E3 glycoprotein
    Gene namesi
    Name:HE
    ORF Names:2b
    OrganismiMurine coronavirus (strain JHM) (MHV-JHM) (Murine hepatitis virus)
    Taxonomic identifieri11144 [NCBI]
    Taxonomic lineageiVirusesssRNA positive-strand viruses, no DNA stageNidoviralesCoronaviridaeCoronavirinaeBetacoronavirus
    Virus hostiMus musculus (Mouse) [TaxID: 10090]
    ProteomesiUP000007193: Genome

    Subcellular locationi

    Virion membrane Curated; Single-pass type I membrane protein Curated. Host cell membrane Curated; Single-pass type I membrane protein Curated
    Note: In infected cells becomes incorporated into the envelope of virions during virus assembly at the endoplasmic reticulum and cis Golgi. However, some may escape incorporation into virions and subsequently migrate to the cell surface By similarity.By similarity

    GO - Cellular componenti

    1. host cell plasma membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW
    3. viral envelope Source: UniProtKB-KW
    4. virion membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Host cell membrane, Host membrane, Membrane, Viral envelope protein, Virion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2222Sequence AnalysisAdd
    BLAST
    Chaini23 – 439417Hemagglutinin-esterasePRO_0000037147Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi49 ↔ 70By similarity
    Glycosylationi94 – 941N-linked (GlcNAc...); by hostSequence Analysis
    Disulfide bondi118 ↔ 167By similarity
    Glycosylationi196 – 1961N-linked (GlcNAc...); by hostSequence Analysis
    Disulfide bondi202 ↔ 291By similarity
    Disulfide bondi210 ↔ 264By similarity
    Glycosylationi246 – 2461N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi309 – 3091N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi316 – 3161N-linked (GlcNAc...); by hostSequence Analysis
    Disulfide bondi322 ↔ 327By similarity
    Glycosylationi331 – 3311N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi360 – 3601N-linked (GlcNAc...); by hostSequence Analysis
    Disulfide bondi363 ↔ 387By similarity
    Glycosylationi374 – 3741N-linked (GlcNAc...); by hostSequence Analysis

    Post-translational modificationi

    N-glycosylated in the RER.

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Interactioni

    Subunit structurei

    Homodimer; disulfide-linked. Forms a complex with the M protein in the pre-Golgi. Associates then with S-M complex to form a ternary complex S-M-HE.

    Structurei

    3D structure databases

    ProteinModelPortaliQ83356.
    SMRiQ83356. Positions 26-392.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini23 – 407385Virion surfaceSequence AnalysisAdd
    BLAST
    Topological domaini429 – 43911IntravirionSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei408 – 42821HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni12 – 132121Esterase domain first partBy similarityAdd
    BLAST
    Regioni133 – 281149Receptor bindingBy similarityAdd
    BLAST
    Regioni282 – 395114Esterase domain second partBy similarityAdd
    BLAST

    Sequence similaritiesi

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane helix

    Family and domain databases

    InterProiIPR008980. Capsid_hemagglutn.
    IPR007142. Hemagglutn-estrase_core.
    IPR003860. Hemagglutn-estrase_hemagglutn.
    [Graphical view]
    PfamiPF03996. Hema_esterase. 1 hit.
    PF02710. Hema_HEFG. 1 hit.
    [Graphical view]
    SUPFAMiSSF49818. SSF49818. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q83356-1 [UniParc]FASTAAdd to Basket

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    MGSTCIAMAP RTLLLLIGCQ LVFGFNEPLN IVSHLNDDWF LFGDSRSDCT    50
    YVENNGHPKL DWLDLDPKLC NSGKISAKSG NSLFRSFHFT DFYNYTGEGD 100
    QIVFYEGVNF SPNHGFKCLA YGDNKRWMGN KARFYARVYE KMAQYRSLSF 150
    VNVPYAYGGK AKPTSICKHK TLTLNNPTFI SKESNYVDYY YESEANFTLA 200
    GCDEFIVPLC VFNGHSKGSS SDPANKYYMD SQSYYNMDTG VLYGFNSTLD 250
    VGNTAKDPGL DLTCRYLALT PGNYKAVSLE YLLSLPSKAI CLRKPKRFMP 300
    VQVVDSRWNS TRQSDNMTAV ACQLPYCFFR NTSADYSGGT HDVHHGDFHF 350
    RQLLSGLLLN VSCIAQQGAF LYNNVSSSWP AYGYGQCPTA ANIGYMAPVC 400
    IYDPLPVVLL GVLLGIAVLI IVFLILYFMT DSGVRLHEA 439
    Length:439
    Mass (Da):49,110
    Last modified:November 1, 1996 - v1
    Checksum:i3E4E70E58D2FB762
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti133 – 1331R → A in AAA46442. (PubMed:2547994)Curated
    Sequence conflicti245 – 2451F → L(PubMed:2547994)Curated
    Sequence conflicti247 – 2471S → C(PubMed:2547994)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D00764 Genomic RNA. Translation: BAA00661.1.
    M28348 Genomic RNA. Translation: AAA46442.1.
    PIRiJQ0997.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D00764 Genomic RNA. Translation: BAA00661.1 .
    M28348 Genomic RNA. Translation: AAA46442.1 .
    PIRi JQ0997.

    3D structure databases

    ProteinModelPortali Q83356.
    SMRi Q83356. Positions 26-392.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR008980. Capsid_hemagglutn.
    IPR007142. Hemagglutn-estrase_core.
    IPR003860. Hemagglutn-estrase_hemagglutn.
    [Graphical view ]
    Pfami PF03996. Hema_esterase. 1 hit.
    PF02710. Hema_HEFG. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49818. SSF49818. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "High level transient expression of the murine coronavirus haemagglutinin-esterase."
      Pfleiderer M., Routledge E., Herrler G., Siddell S.G.
      J. Gen. Virol. 72:1309-1315(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    2. "Identification of a new transcriptional initiation site and the corresponding functional gene 2b in the murine coronavirus RNA genome."
      Shieh C.-K., Lee H.-J., Yokomori K., la Monica N., Makino S., Lai M.M.C.
      J. Virol. 63:3729-3736(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    3. "Functional analysis of the coronavirus MHV-JHM surface glycoproteins in vaccinia virus recombinants."
      Pfleiderer M., Routledge E., Siddell S.G.
      Adv. Exp. Med. Biol. 276:21-31(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    4. "The sialate-4-O-acetylesterases of coronaviruses related to mouse hepatitis virus: a proposal to reorganize group 2 Coronaviridae."
      Wurzer W.J., Obojes K., Vlasak R.
      J. Gen. Virol. 83:395-402(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.

    Entry informationi

    Entry nameiHEMA_CVMJH
    AccessioniPrimary (citable) accession number: Q83356
    Secondary accession number(s): Q83353
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 15, 1998
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 88 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3