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Protein

Hemagglutinin-esterase

Gene

HE

Organism
Murine coronavirus (strain JHM) (MHV-JHM) (Murine hepatitis virus)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Structural protein that makes short spikes at the surface of the virus. Contains receptor binding and receptor-destroying activities. Mediates de-O-acetylation of N-acetyl-4-O-acetylneuraminic acid, which is probably the receptor determinant recognized by the virus on the surface of erythrocytes and susceptible cells. This receptor-destroying activity is important for virus release as it probably helps preventing self-aggregation and ensures the efficient spread of the progeny virus from cell to cell. May serve as a secondary viral attachment protein for initiating infection, the spike protein being the major one. Seems to be a 'luxury' protein that is not absolutely necessary for virus infection in culture. However, its presence in the virus may alter its pathogenicity. May become a target for both the humoral and the cellular branches of the immune system.

Catalytic activityi

N-acetyl-O-acetylneuraminate + H2O = N-acetylneuraminate + acetate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei45NucleophileBy similarity1
Active sitei342Charge relay systemBy similarity1
Active sitei345Charge relay systemBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hemagglutinin, Hydrolase

Names & Taxonomyi

Protein namesi
Recommended name:
Hemagglutinin-esterase (EC:3.1.1.53)
Short name:
HE protein
Alternative name(s):
E3 glycoprotein
Gene namesi
Name:HE
ORF Names:2b
OrganismiMurine coronavirus (strain JHM) (MHV-JHM) (Murine hepatitis virus)
Taxonomic identifieri11144 [NCBI]
Taxonomic lineageiVirusesssRNA virusesssRNA positive-strand viruses, no DNA stageNidoviralesCoronaviridaeCoronavirinaeBetacoronavirus
Virus hostiMus musculus (Mouse) [TaxID: 10090]
Proteomesi
  • UP000007193 Componenti: Genome

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini23 – 407Virion surfaceSequence analysisAdd BLAST385
Transmembranei408 – 428HelicalSequence analysisAdd BLAST21
Topological domaini429 – 439IntravirionSequence analysisAdd BLAST11

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Host cell membrane, Host membrane, Membrane, Viral envelope protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 22Sequence analysisAdd BLAST22
ChainiPRO_000003714723 – 439Hemagglutinin-esteraseAdd BLAST417

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi49 ↔ 70By similarity
Glycosylationi94N-linked (GlcNAc...); by hostSequence analysis1
Disulfide bondi118 ↔ 167By similarity
Glycosylationi196N-linked (GlcNAc...); by hostSequence analysis1
Disulfide bondi202 ↔ 291By similarity
Disulfide bondi210 ↔ 264By similarity
Glycosylationi246N-linked (GlcNAc...); by hostSequence analysis1
Glycosylationi309N-linked (GlcNAc...); by hostSequence analysis1
Glycosylationi316N-linked (GlcNAc...); by hostSequence analysis1
Disulfide bondi322 ↔ 327By similarity
Glycosylationi331N-linked (GlcNAc...); by hostSequence analysis1
Glycosylationi360N-linked (GlcNAc...); by hostSequence analysis1
Disulfide bondi363 ↔ 387By similarity
Glycosylationi374N-linked (GlcNAc...); by hostSequence analysis1

Post-translational modificationi

N-glycosylated in the RER.

Keywords - PTMi

Disulfide bond, Glycoprotein

Interactioni

Subunit structurei

Homodimer; disulfide-linked. Forms a complex with the M protein in the pre-Golgi. Associates then with S-M complex to form a ternary complex S-M-HE.

Structurei

3D structure databases

ProteinModelPortaliQ83356.
SMRiQ83356.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni12 – 132Esterase domain first partBy similarityAdd BLAST121
Regioni133 – 281Receptor bindingBy similarityAdd BLAST149
Regioni282 – 395Esterase domain second partBy similarityAdd BLAST114

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Family and domain databases

InterProiIPR008980. Capsid_hemagglutn.
IPR007142. Hemagglutn-estrase_core.
IPR003860. Hemagglutn-estrase_hemagglutn.
IPR013830. SGNH_hydro.
[Graphical view]
PfamiPF03996. Hema_esterase. 1 hit.
PF02710. Hema_HEFG. 1 hit.
[Graphical view]
SUPFAMiSSF49818. SSF49818. 1 hit.
SSF52266. SSF52266. 2 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q83356-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGSTCIAMAP RTLLLLIGCQ LVFGFNEPLN IVSHLNDDWF LFGDSRSDCT
60 70 80 90 100
YVENNGHPKL DWLDLDPKLC NSGKISAKSG NSLFRSFHFT DFYNYTGEGD
110 120 130 140 150
QIVFYEGVNF SPNHGFKCLA YGDNKRWMGN KARFYARVYE KMAQYRSLSF
160 170 180 190 200
VNVPYAYGGK AKPTSICKHK TLTLNNPTFI SKESNYVDYY YESEANFTLA
210 220 230 240 250
GCDEFIVPLC VFNGHSKGSS SDPANKYYMD SQSYYNMDTG VLYGFNSTLD
260 270 280 290 300
VGNTAKDPGL DLTCRYLALT PGNYKAVSLE YLLSLPSKAI CLRKPKRFMP
310 320 330 340 350
VQVVDSRWNS TRQSDNMTAV ACQLPYCFFR NTSADYSGGT HDVHHGDFHF
360 370 380 390 400
RQLLSGLLLN VSCIAQQGAF LYNNVSSSWP AYGYGQCPTA ANIGYMAPVC
410 420 430
IYDPLPVVLL GVLLGIAVLI IVFLILYFMT DSGVRLHEA
Length:439
Mass (Da):49,110
Last modified:November 1, 1996 - v1
Checksum:i3E4E70E58D2FB762
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti133R → A in AAA46442 (PubMed:2547994).Curated1
Sequence conflicti245F → L (PubMed:2547994).Curated1
Sequence conflicti247S → C (PubMed:2547994).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D00764 Genomic RNA. Translation: BAA00661.1.
M28348 Genomic RNA. Translation: AAA46442.1.
PIRiJQ0997.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D00764 Genomic RNA. Translation: BAA00661.1.
M28348 Genomic RNA. Translation: AAA46442.1.
PIRiJQ0997.

3D structure databases

ProteinModelPortaliQ83356.
SMRiQ83356.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

InterProiIPR008980. Capsid_hemagglutn.
IPR007142. Hemagglutn-estrase_core.
IPR003860. Hemagglutn-estrase_hemagglutn.
IPR013830. SGNH_hydro.
[Graphical view]
PfamiPF03996. Hema_esterase. 1 hit.
PF02710. Hema_HEFG. 1 hit.
[Graphical view]
SUPFAMiSSF49818. SSF49818. 1 hit.
SSF52266. SSF52266. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiHEMA_CVMJH
AccessioniPrimary (citable) accession number: Q83356
Secondary accession number(s): Q83353
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: November 1, 1996
Last modified: November 2, 2016
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.