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Q83356

- HEMA_CVMJH

UniProt

Q83356 - HEMA_CVMJH

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Protein

Hemagglutinin-esterase

Gene

HE

Organism
Murine coronavirus (strain JHM) (MHV-JHM) (Murine hepatitis virus)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Structural protein that makes short spikes at the surface of the virus. Contains receptor binding and receptor-destroying activities. Mediates de-O-acetylation of N-acetyl-4-O-acetylneuraminic acid, which is probably the receptor determinant recognized by the virus on the surface of erythrocytes and susceptible cells. This receptor-destroying activity is important for virus release as it probably helps preventing self-aggregation and ensures the efficient spread of the progeny virus from cell to cell. May serve as a secondary viral attachment protein for initiating infection, the spike protein being the major one. Seems to be a 'luxury' protein that is not absolutely necessary for virus infection in culture. However, its presence in the virus may alter its pathogenicity. May become a target for both the humoral and the cellular branches of the immune system.

Catalytic activityi

N-acetyl-O-acetylneuraminate + H2O = N-acetylneuraminate + acetate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei45 – 451NucleophileBy similarity
Active sitei342 – 3421Charge relay systemBy similarity
Active sitei345 – 3451Charge relay systemBy similarity

GO - Molecular functioni

  1. sialate O-acetylesterase activity Source: UniProtKB-EC

GO - Biological processi

  1. fusion of virus membrane with host plasma membrane Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hemagglutinin, Hydrolase

Names & Taxonomyi

Protein namesi
Recommended name:
Hemagglutinin-esterase (EC:3.1.1.53)
Short name:
HE protein
Alternative name(s):
E3 glycoprotein
Gene namesi
Name:HE
ORF Names:2b
OrganismiMurine coronavirus (strain JHM) (MHV-JHM) (Murine hepatitis virus)
Taxonomic identifieri11144 [NCBI]
Taxonomic lineageiVirusesssRNA positive-strand viruses, no DNA stageNidoviralesCoronaviridaeCoronavirinaeBetacoronavirus
Virus hostiMus musculus (Mouse) [TaxID: 10090]
ProteomesiUP000007193: Genome

Subcellular locationi

Virion membrane Curated; Single-pass type I membrane protein Curated. Host cell membrane Curated; Single-pass type I membrane protein Curated
Note: In infected cells becomes incorporated into the envelope of virions during virus assembly at the endoplasmic reticulum and cis Golgi. However, some may escape incorporation into virions and subsequently migrate to the cell surface (By similarity).By similarity

GO - Cellular componenti

  1. host cell plasma membrane Source: UniProtKB-KW
  2. integral component of membrane Source: UniProtKB-KW
  3. viral envelope Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Host cell membrane, Host membrane, Membrane, Viral envelope protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222Sequence AnalysisAdd
BLAST
Chaini23 – 439417Hemagglutinin-esterasePRO_0000037147Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi49 ↔ 70By similarity
Glycosylationi94 – 941N-linked (GlcNAc...); by hostSequence Analysis
Disulfide bondi118 ↔ 167By similarity
Glycosylationi196 – 1961N-linked (GlcNAc...); by hostSequence Analysis
Disulfide bondi202 ↔ 291By similarity
Disulfide bondi210 ↔ 264By similarity
Glycosylationi246 – 2461N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi309 – 3091N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi316 – 3161N-linked (GlcNAc...); by hostSequence Analysis
Disulfide bondi322 ↔ 327By similarity
Glycosylationi331 – 3311N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi360 – 3601N-linked (GlcNAc...); by hostSequence Analysis
Disulfide bondi363 ↔ 387By similarity
Glycosylationi374 – 3741N-linked (GlcNAc...); by hostSequence Analysis

Post-translational modificationi

N-glycosylated in the RER.

Keywords - PTMi

Disulfide bond, Glycoprotein

Interactioni

Subunit structurei

Homodimer; disulfide-linked. Forms a complex with the M protein in the pre-Golgi. Associates then with S-M complex to form a ternary complex S-M-HE.

Structurei

3D structure databases

ProteinModelPortaliQ83356.
SMRiQ83356. Positions 26-392.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini23 – 407385Virion surfaceSequence AnalysisAdd
BLAST
Topological domaini429 – 43911IntravirionSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei408 – 42821HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni12 – 132121Esterase domain first partBy similarityAdd
BLAST
Regioni133 – 281149Receptor bindingBy similarityAdd
BLAST
Regioni282 – 395114Esterase domain second partBy similarityAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Family and domain databases

InterProiIPR008980. Capsid_hemagglutn.
IPR007142. Hemagglutn-estrase_core.
IPR003860. Hemagglutn-estrase_hemagglutn.
[Graphical view]
PfamiPF03996. Hema_esterase. 1 hit.
PF02710. Hema_HEFG. 1 hit.
[Graphical view]
SUPFAMiSSF49818. SSF49818. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q83356-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGSTCIAMAP RTLLLLIGCQ LVFGFNEPLN IVSHLNDDWF LFGDSRSDCT
60 70 80 90 100
YVENNGHPKL DWLDLDPKLC NSGKISAKSG NSLFRSFHFT DFYNYTGEGD
110 120 130 140 150
QIVFYEGVNF SPNHGFKCLA YGDNKRWMGN KARFYARVYE KMAQYRSLSF
160 170 180 190 200
VNVPYAYGGK AKPTSICKHK TLTLNNPTFI SKESNYVDYY YESEANFTLA
210 220 230 240 250
GCDEFIVPLC VFNGHSKGSS SDPANKYYMD SQSYYNMDTG VLYGFNSTLD
260 270 280 290 300
VGNTAKDPGL DLTCRYLALT PGNYKAVSLE YLLSLPSKAI CLRKPKRFMP
310 320 330 340 350
VQVVDSRWNS TRQSDNMTAV ACQLPYCFFR NTSADYSGGT HDVHHGDFHF
360 370 380 390 400
RQLLSGLLLN VSCIAQQGAF LYNNVSSSWP AYGYGQCPTA ANIGYMAPVC
410 420 430
IYDPLPVVLL GVLLGIAVLI IVFLILYFMT DSGVRLHEA
Length:439
Mass (Da):49,110
Last modified:November 1, 1996 - v1
Checksum:i3E4E70E58D2FB762
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti133 – 1331R → A in AAA46442. (PubMed:2547994)Curated
Sequence conflicti245 – 2451F → L(PubMed:2547994)Curated
Sequence conflicti247 – 2471S → C(PubMed:2547994)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D00764 Genomic RNA. Translation: BAA00661.1.
M28348 Genomic RNA. Translation: AAA46442.1.
PIRiJQ0997.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D00764 Genomic RNA. Translation: BAA00661.1 .
M28348 Genomic RNA. Translation: AAA46442.1 .
PIRi JQ0997.

3D structure databases

ProteinModelPortali Q83356.
SMRi Q83356. Positions 26-392.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR008980. Capsid_hemagglutn.
IPR007142. Hemagglutn-estrase_core.
IPR003860. Hemagglutn-estrase_hemagglutn.
[Graphical view ]
Pfami PF03996. Hema_esterase. 1 hit.
PF02710. Hema_HEFG. 1 hit.
[Graphical view ]
SUPFAMi SSF49818. SSF49818. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "High level transient expression of the murine coronavirus haemagglutinin-esterase."
    Pfleiderer M., Routledge E., Herrler G., Siddell S.G.
    J. Gen. Virol. 72:1309-1315(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  2. "Identification of a new transcriptional initiation site and the corresponding functional gene 2b in the murine coronavirus RNA genome."
    Shieh C.-K., Lee H.-J., Yokomori K., la Monica N., Makino S., Lai M.M.C.
    J. Virol. 63:3729-3736(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  3. "Functional analysis of the coronavirus MHV-JHM surface glycoproteins in vaccinia virus recombinants."
    Pfleiderer M., Routledge E., Siddell S.G.
    Adv. Exp. Med. Biol. 276:21-31(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  4. "The sialate-4-O-acetylesterases of coronaviruses related to mouse hepatitis virus: a proposal to reorganize group 2 Coronaviridae."
    Wurzer W.J., Obojes K., Vlasak R.
    J. Gen. Virol. 83:395-402(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.

Entry informationi

Entry nameiHEMA_CVMJH
AccessioniPrimary (citable) accession number: Q83356
Secondary accession number(s): Q83353
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: November 1, 1996
Last modified: October 29, 2014
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3