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Q83356

- HEMA_CVMJH

UniProt

Q83356 - HEMA_CVMJH

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Protein

Hemagglutinin-esterase

Gene
HE, 2b
Organism
Murine coronavirus (strain JHM) (MHV-JHM) (Murine hepatitis virus)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Structural protein that makes short spikes at the surface of the virus. Contains receptor binding and receptor-destroying activities. Mediates de-O-acetylation of N-acetyl-4-O-acetylneuraminic acid, which is probably the receptor determinant recognized by the virus on the surface of erythrocytes and susceptible cells. This receptor-destroying activity is important for virus release as it probably helps preventing self-aggregation and ensures the efficient spread of the progeny virus from cell to cell. May serve as a secondary viral attachment protein for initiating infection, the spike protein being the major one. Seems to be a 'luxury' protein that is not absolutely necessary for virus infection in culture. However, its presence in the virus may alter its pathogenicity. May become a target for both the humoral and the cellular branches of the immune system.

Catalytic activityi

N-acetyl-O-acetylneuraminate + H2O = N-acetylneuraminate + acetate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei45 – 451Nucleophile By similarity
Active sitei238 – 2381Charge relay system By similarity
Active sitei345 – 3451Charge relay system By similarity

GO - Molecular functioni

  1. sialate O-acetylesterase activity Source: UniProtKB-EC

GO - Biological processi

  1. fusion of virus membrane with host plasma membrane Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hemagglutinin, Hydrolase

Names & Taxonomyi

Protein namesi
Recommended name:
Hemagglutinin-esterase (EC:3.1.1.53)
Short name:
HE protein
Alternative name(s):
E3 glycoprotein
Gene namesi
Name:HE
ORF Names:2b
OrganismiMurine coronavirus (strain JHM) (MHV-JHM) (Murine hepatitis virus)
Taxonomic identifieri11144 [NCBI]
Taxonomic lineageiVirusesssRNA positive-strand viruses, no DNA stageNidoviralesCoronaviridaeCoronavirinaeBetacoronavirus
Virus hostiMus musculus (Mouse) [TaxID: 10090]
ProteomesiUP000007193: Genome

Subcellular locationi

Virion membrane; Single-pass type I membrane protein Reviewed prediction. Host cell membrane; Single-pass type I membrane protein Reviewed prediction
Note: In infected cells becomes incorporated into the envelope of virions during virus assembly at the endoplasmic reticulum and cis Golgi. However, some may escape incorporation into virions and subsequently migrate to the cell surface By similarity.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini23 – 407385Virion surface Reviewed predictionAdd
BLAST
Transmembranei408 – 42821Helical; Reviewed predictionAdd
BLAST
Topological domaini429 – 43911Intravirion Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. host cell plasma membrane Source: UniProtKB-SubCell
  2. integral component of membrane Source: UniProtKB-KW
  3. viral envelope Source: UniProtKB-KW
  4. virion membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Host cell membrane, Host membrane, Membrane, Viral envelope protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222 Reviewed predictionAdd
BLAST
Chaini23 – 439417Hemagglutinin-esterasePRO_0000037147Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi49 ↔ 70 By similarity
Glycosylationi94 – 941N-linked (GlcNAc...); by host Reviewed prediction
Disulfide bondi118 ↔ 167 By similarity
Glycosylationi196 – 1961N-linked (GlcNAc...); by host Reviewed prediction
Disulfide bondi202 ↔ 291 By similarity
Disulfide bondi210 ↔ 264 By similarity
Glycosylationi246 – 2461N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi309 – 3091N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi316 – 3161N-linked (GlcNAc...); by host Reviewed prediction
Disulfide bondi322 ↔ 327 By similarity
Glycosylationi331 – 3311N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi360 – 3601N-linked (GlcNAc...); by host Reviewed prediction
Disulfide bondi363 ↔ 387 By similarity
Glycosylationi374 – 3741N-linked (GlcNAc...); by host Reviewed prediction

Post-translational modificationi

N-glycosylated in the RER.

Keywords - PTMi

Disulfide bond, Glycoprotein

Interactioni

Subunit structurei

Homodimer; disulfide-linked. Forms a complex with the M protein in the pre-Golgi. Associates then with S-M complex to form a ternary complex S-M-HE.

Structurei

3D structure databases

ProteinModelPortaliQ83356.
SMRiQ83356. Positions 26-392.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni12 – 132121Esterase domain first part By similarityAdd
BLAST
Regioni133 – 281149Receptor binding By similarityAdd
BLAST
Regioni282 – 395114Esterase domain second part By similarityAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Family and domain databases

InterProiIPR008980. Capsid_hemagglutn.
IPR007142. Hemagglutn-estrase_core.
IPR003860. Hemagglutn-estrase_hemagglutn.
[Graphical view]
PfamiPF03996. Hema_esterase. 1 hit.
PF02710. Hema_HEFG. 1 hit.
[Graphical view]
SUPFAMiSSF49818. SSF49818. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q83356-1 [UniParc]FASTAAdd to Basket

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MGSTCIAMAP RTLLLLIGCQ LVFGFNEPLN IVSHLNDDWF LFGDSRSDCT    50
YVENNGHPKL DWLDLDPKLC NSGKISAKSG NSLFRSFHFT DFYNYTGEGD 100
QIVFYEGVNF SPNHGFKCLA YGDNKRWMGN KARFYARVYE KMAQYRSLSF 150
VNVPYAYGGK AKPTSICKHK TLTLNNPTFI SKESNYVDYY YESEANFTLA 200
GCDEFIVPLC VFNGHSKGSS SDPANKYYMD SQSYYNMDTG VLYGFNSTLD 250
VGNTAKDPGL DLTCRYLALT PGNYKAVSLE YLLSLPSKAI CLRKPKRFMP 300
VQVVDSRWNS TRQSDNMTAV ACQLPYCFFR NTSADYSGGT HDVHHGDFHF 350
RQLLSGLLLN VSCIAQQGAF LYNNVSSSWP AYGYGQCPTA ANIGYMAPVC 400
IYDPLPVVLL GVLLGIAVLI IVFLILYFMT DSGVRLHEA 439
Length:439
Mass (Da):49,110
Last modified:November 1, 1996 - v1
Checksum:i3E4E70E58D2FB762
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti133 – 1331R → A in AAA46442. 1 Publication
Sequence conflicti245 – 2451F → L1 Publication
Sequence conflicti247 – 2471S → C1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D00764 Genomic RNA. Translation: BAA00661.1.
M28348 Genomic RNA. Translation: AAA46442.1.
PIRiJQ0997.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D00764 Genomic RNA. Translation: BAA00661.1 .
M28348 Genomic RNA. Translation: AAA46442.1 .
PIRi JQ0997.

3D structure databases

ProteinModelPortali Q83356.
SMRi Q83356. Positions 26-392.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR008980. Capsid_hemagglutn.
IPR007142. Hemagglutn-estrase_core.
IPR003860. Hemagglutn-estrase_hemagglutn.
[Graphical view ]
Pfami PF03996. Hema_esterase. 1 hit.
PF02710. Hema_HEFG. 1 hit.
[Graphical view ]
SUPFAMi SSF49818. SSF49818. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "High level transient expression of the murine coronavirus haemagglutinin-esterase."
    Pfleiderer M., Routledge E., Herrler G., Siddell S.G.
    J. Gen. Virol. 72:1309-1315(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  2. "Identification of a new transcriptional initiation site and the corresponding functional gene 2b in the murine coronavirus RNA genome."
    Shieh C.-K., Lee H.-J., Yokomori K., la Monica N., Makino S., Lai M.M.C.
    J. Virol. 63:3729-3736(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  3. "Functional analysis of the coronavirus MHV-JHM surface glycoproteins in vaccinia virus recombinants."
    Pfleiderer M., Routledge E., Siddell S.G.
    Adv. Exp. Med. Biol. 276:21-31(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  4. "The sialate-4-O-acetylesterases of coronaviruses related to mouse hepatitis virus: a proposal to reorganize group 2 Coronaviridae."
    Wurzer W.J., Obojes K., Vlasak R.
    J. Gen. Virol. 83:395-402(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.

Entry informationi

Entry nameiHEMA_CVMJH
AccessioniPrimary (citable) accession number: Q83356
Secondary accession number(s): Q83353
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: November 1, 1996
Last modified: April 16, 2014
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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