Q832Z5 (KPRS2_ENTFA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribose-phosphate pyrophosphokinase 2
Short name=RPPK 2
EC=2.7.6.1

Alternative name(s):
Phosphoribosyl pyrophosphate synthase 2
Short name=P-Rib-PP synthase 2
Short name=PRPP synthase 2

Gene names

Name:	prs2
Synonyms:	prsA-1
Ordered Locus Names:	EF_2073

Organism

Enterococcus faecalis (Streptococcus faecalis)

Taxonomic identifier

1351 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Enterococcaceae › Enterococcus

Protein attributes

Sequence length	324 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + D-ribose 5-phosphate = AMP + 5-phospho-alpha-D-ribose 1-diphosphate. HAMAP MF_00583_B
Cofactor	Binds 1 magnesium ion per subunit By similarity. HAMAP MF_00583_B
Pathway	Metabolic intermediate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate from D-ribose 5-phosphate (route I): step 1/1. HAMAP MF_00583_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00583_B.
Sequence similarities	Belongs to the ribose-phosphate pyrophosphokinase family.

Ontologies

Keywords
Biological process	Nucleotide biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	nucleoside metabolic process Inferred from electronic annotation. Source: InterPro ribonucleoside monophosphate biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW kinase activity Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: InterPro ribose phosphate diphosphokinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 324	324	Ribose-phosphate pyrophosphokinase 2 HAMAP MF_00583_B		PRO_0000141138
Regions
	Region	218 – 231	14	Binding of phosphoribosylpyrophosphate Potential
Sites
	Metal binding	134	1	Magnesium Potential
	Metal binding	136	1	Magnesium Potential
	Metal binding	145	1	Magnesium Potential
	Metal binding	149	1	Magnesium Potential

Sequences

	Sequence		Length	Mass (Da)
	Q832Z5 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: AFA380343F90EB60	FASTA	324	35,591
	10 20 30 40 50 60 MTENYQDDTL RIFSLNANRP LAEKIAASVG TELGKSTVRQ FSDGEIQINI EESIRGDHVY 70 80 90 100 110 120 IIQATNAPVN DHLMELLILI DALKRASAKT VNVILPYYGY ARQDRTAKPR EPITAKLVAN 130 140 150 160 170 180 MLVEAGATRL LTLDLHTVQV QGFFDIPVDN LFTMPLFAHY YRQQALVGEE IVIVSPKNSG 190 200 210 220 230 240 VQRARSLSEY LDATLAIVDH EEIDGVRQEG YVIGNVAGKK CILVDDILNT GQTLATAAEV 250 260 270 280 290 300 LMKNGAQEVY ACASHGLLSE GAKATLENAP IKEISITDSV YTTADRQPAT LNIISCAELM 310 320 GEALLRIHEN KPMSPLFRLE PKGE

References

[1]

"Role of mobile DNA in the evolution of vancomycin-resistant Enterococcus faecalis." Paulsen I.T., Banerjei L., Myers G.S.A., Nelson K.E., Seshadri R., Read T.D., Fouts D.E., Eisen J.A., Gill S.R., Heidelberg J.F., Tettelin H., Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J., Daugherty S.C., DeBoy R.T., Durkin S.A. , Kolonay J.F., Madupu R., Nelson W.C., Vamathevan J.J., Tran B., Upton J., Hansen T., Shetty J., Khouri H.M., Utterback T.R., Radune D., Ketchum K.A., Dougherty B.A., Fraser C.M. Science 299:2071-2074(2003) [PubMed: 12663927] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700802 / V583.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016830 Genomic DNA. Translation: AAO81806.1.
RefSeq	NP_815736.1. NC_004668.1.
3D structure databases
ProteinModelPortal	Q832Z5.
SMR	Q832Z5. Positions 10-318.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1200945.
GenomeReviews	Gene locus EF_2073 in contig AE016830_GR.
KEGG	efa:EF2073.
NMPDR	fig|226185.1.peg.1921.
PATRIC	21854440. VBIEntFae7065_1944.
TIGR	EF_2073.
Phylogenomic databases
HOGENOM	HBG519284.
OMA	CTSGLTH.
ProtClustDB	PRK02458.
Enzyme and pathway databases
BioCyc	EFAE226185:EF_2073-MONOMER.
Family and domain databases
HAMAP	MF_00583_B. RibP_PPkinase_B. [Tree]
InterPro	IPR000842. PRib_PP_synth_CS. IPR000836. PRibTrfase. IPR005946. Rib-P_diPkinase. [Graphical view]
KO	K00948.
Pfam	PF00156. Pribosyltran. 1 hit. [Graphical view]
TIGRFAMs	TIGR01251. RibP_PPkin. 1 hit.
PROSITE	PS00114. PRPP_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KPRS2_ENTFA

Accession

Primary (citable) accession number: Q832Z5

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 13, 2004
Last sequence update:	June 1, 2003
Last modified:	January 25, 2012
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program