Q831X4 (SYN_ENTFA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Asparagine--tRNA ligase
EC=6.1.1.22

Alternative name(s):
Asparaginyl-tRNA synthetase
Short name=AsnRS

Gene names

Name:	asnS
Ordered Locus Names:	EF_2371

Organism

Enterococcus faecalis (Streptococcus faecalis)

Taxonomic identifier

1351 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Enterococcaceae › Enterococcus

Protein attributes

Sequence length	450 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-asparagine + tRNA(Asn) = AMP + diphosphate + L-asparaginyl-tRNA(Asn). HAMAP MF_00534
Subunit structure	Homodimer By similarity. HAMAP MF_00534
Subcellular location	Cytoplasm HAMAP MF_00534.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	asparaginyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW asparagine-tRNA ligase activity Inferred from electronic annotation. Source: EC nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 450	450	Asparagine--tRNA ligase HAMAP MF_00534		PRO_0000176409

Sequences

	Sequence		Length	Mass (Da)
	Q831X4 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: A1D8970F5DAB108C	FASTA	450	51,492
	10 20 30 40 50 60 MNGETNVEQI QIIDSNKHVG ETVKIGAWIA NKRSSGKIAF LQLRDGTAFF QGVVFKPNFI 70 80 90 100 110 120 EAFGEEAGTE KFQEIKHLSQ ETAVMVTGVI KEDSRSKFGY EMDITDLEVV GASEDYPITP 130 140 150 160 170 180 KEHGTDFLMD HRHLWLRSSK QHAIMLVRNE IIRATYEFFN EQGFIKIDSP ILTGSAPEGT 190 200 210 220 230 240 TELFETDYFG QPAFLSQTGQ LYAEAGAMAF GKVFTFGPTF RAEKSKTRRH LTEFWMIEPE 250 260 270 280 290 300 MAYTTHEESL DIQEAYVKHL IKSVLKNQQY PLDVLERDTA LLEKYVSEPF KRITYDDAIE 310 320 330 340 350 360 LLQKEEANND YDHIEWGEDF GSPHETFISN YYGVPTFILN YPKAIKAFYM KPHPTREDVV 370 380 390 400 410 420 ICADLIAPEG YGEIIGGSER ATDYDYLKEK VAEFGLSEEE YSWYLDLRKY GSVPHSGFGL 430 440 450 GLERAVTFIT GNEHIREAIP FPRMLNRIYP

References

[1]

"Role of mobile DNA in the evolution of vancomycin-resistant Enterococcus faecalis." Paulsen I.T., Banerjei L., Myers G.S.A., Nelson K.E., Seshadri R., Read T.D., Fouts D.E., Eisen J.A., Gill S.R., Heidelberg J.F., Tettelin H., Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J., Daugherty S.C., DeBoy R.T., Durkin S.A. , Kolonay J.F., Madupu R., Nelson W.C., Vamathevan J.J., Tran B., Upton J., Hansen T., Shetty J., Khouri H.M., Utterback T.R., Radune D., Ketchum K.A., Dougherty B.A., Fraser C.M. Science 299:2071-2074(2003) [PubMed: 12663927] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700802 / V583.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016830 Genomic DNA. Translation: AAO82093.1.
RefSeq	NP_816023.1. NC_004668.1.
3D structure databases
ProteinModelPortal	Q831X4.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1201240.
GenomeReviews	Gene locus EF_2371 in contig AE016830_GR.
KEGG	efa:EF2371.
NMPDR	fig|226185.1.peg.2208.
PATRIC	21854997. VBIEntFae7065_2219.
TIGR	EF_2371.
Phylogenomic databases
HOGENOM	HBG745843.
OMA	AVDYPIT.
ProtClustDB	PRK03932.
Enzyme and pathway databases
BioCyc	EFAE226185:EF_2371-MONOMER.
Family and domain databases
HAMAP	MF_00534. Asn_tRNA_synth. [Tree]
InterPro	IPR004364. aa-tRNA-synt_II. IPR018150. aa-tRNA-synt_II-like. IPR006195. aa-tRNA-synth_II. IPR004522. Asn-tRNA-synth_IIb. IPR002312. Asp/Asn-tRNA-synth_IIb. IPR012340. NA-bd_OB-fold. IPR016027. NA-bd_OB-fold-like. IPR004365. NA-bd_OB_tRNA-helicase. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
KO	K01893.
PANTHER	PTHR22594. aa-tRNA-synt_II. 1 hit. PTHR22594:SF6. PTHR22594:SF6. 1 hit.
Pfam	PF00152. tRNA-synt_2. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
PRINTS	PR01042. TRNASYNTHASP.
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit.
TIGRFAMs	TIGR00457. AsnS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYN_ENTFA

Accession

Primary (citable) accession number: Q831X4

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2003
Last sequence update:	June 1, 2003
Last modified:	January 25, 2012
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program