Reviewed, UniProtKB/Swiss-Prot Q831S1 (GUAC_ENTFA)

Names and origin

Protein names

Recommended name:
    GMP reductase
    EC=1.7.1.7
Alternative name(s):
    Guanosine 5'-monophosphate oxidoreductase
      Short name=Guanosine monophosphate reductase

Gene names

Name:	guaC
Ordered Locus Names:	EF_2429

Organism

Enterococcus faecalis (Streptococcus faecalis) [Complete proteome] [HAMAP]

Taxonomic identifier

1351 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Enterococcaceae › Enterococcus

Protein attributes

Sequence length	325 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the irreversible NADPH-dependent deamination of GMP to IMP. It functions in the conversion of nucleobase, nucleoside and nucleotide derivatives of G to A nucleotides, and in maintaining the intracellular balance of A and G nucleotides By similarity. HAMAP MF_01511
Catalytic activity	Inosine 5'-phosphate + NH3 + NADP+ = guanosine 5'-phosphate + NADPH. HAMAP MF_01511
Sequence similarities	Belongs to the IMPDH/GMPR family. GuaC type 2 subfamily.

Ontologies

Keywords
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW purine nucleotide metabolic process Inferred from electronic annotation. Source: HAMAP
Molecular function	GMP reductase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 325	325	GMP reductase HAMAP MF_01511		PRO_0000093755
Regions
	Nucleotide binding	203 – 226	24	NADP By similarity
Sites
	Active site	174	1	Thioimidate intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q831S1-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 1B87D44EBEA2F842	FASTA	325	35,646
	10 20 30 40 50 60 MKVFDYEDVQ LIPNKCIVNS RSECDTTVTL GKHSFKMPVV PANMQTIIDE TIAETLAENG 70 80 90 100 110 120 YFYIMHRFDE EARVPFIKKM QQKGLITSIS VGVKEGEYAF VETLAREGLV PDYVTIDIAH 130 140 150 160 170 180 GHSNAVINMI QHLKKSLPET FVIAGNVGTP EAVRELENAG ADATKVGIGP GKVCITKIKT 190 200 210 220 230 240 GFGTGGWQLA ALRWCAKAAR KPIIADGGIR THGDIAKSVR FGATMVMIGS LFAGHEESPG 250 260 270 280 290 300 ETKVEDGVVY KEYFGSASEF QKGEKKNVEG KKIWLRHKGK LADTLVEMQQ DLQSSISYAG 310 320 GRDLEAIRKV DYVIVKNSIF NGDTI

References

[1]

"Role of mobile DNA in the evolution of vancomycin-resistant Enterococcus faecalis." Paulsen I.T., Banerjei L., Myers G.S.A., Nelson K.E., Seshadri R., Read T.D., Fouts D.E., Eisen J.A., Gill S.R., Heidelberg J.F., Tettelin H., Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J., Daugherty S.C., DeBoy R.T., Durkin S.A. , Kolonay J.F., Madupu R., Nelson W.C., Vamathevan J.J., Tran B., Upton J., Hansen T., Shetty J., Khouri H.M., Utterback T.R., Radune D., Ketchum K.A., Dougherty B.A., Fraser C.M. Science 299:2071-2074(2003) [PubMed: 12663927] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: V583 / ATCC 700802.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016830 Genomic DNA. Translation: AAO82147.1.
RefSeq	NP_816077.1.
3D structure databases
SMR	Q831S1. Positions 3-320.
ModBase	Search...
Genome annotation databases
GeneID	1201295.
GenomeReviews	Gene locus EF_2429 in contig AE016830_GR.
KEGG	efa:EF2429.
NMPDR	fig|226185.1.peg.2262.
TIGR	EF_2429.
Phylogenomic databases
HOGENOM	HBG298985.
OMA	FAGHEES.
Enzyme and pathway databases
BRENDA	1.7.1.7. 704.
Family and domain databases
HAMAP	MF_01511. GMP_reduct_type2. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR005994. GMP_reduct2. IPR015875. IMP_DH/GMP_Rdtase_CS. IPR001093. IMP_DH_GMPRt. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
Pfam	PF00478. IMPDH. 1 hit. [Graphical view]
PIRSF	PIRSF036500. GMP_red_Firmic. 1 hit.
TIGRFAMs	TIGR01306. GMP_reduct_2. 1 hit.
PROSITE	PS00487. IMP_DH_GMP_RED. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUAC_ENTFA

Accession

Primary (citable) accession number: Q831S1

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2004
Last sequence update:	June 1, 2003
Last modified:	February 9, 2010
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)