Q831A8 (MURA2_ENTFA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-N-acetylglucosamine 1-carboxyvinyltransferase 2
EC=2.5.1.7

Alternative name(s):
Enoylpyruvate transferase 2
UDP-N-acetylglucosamine enolpyruvyl transferase 2
Short name=EPT 2

Gene names

Name:	murA2
Synonyms:	murAA
Ordered Locus Names:	EF_2605

Organism

Enterococcus faecalis (Streptococcus faecalis)

Taxonomic identifier

1351 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Enterococcaceae › Enterococcus

Protein attributes

Sequence length	433 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Cell wall formation. Adds enolpyruvyl to UDP-N-acetylglucosamine By similarity. HAMAP MF_00111
Catalytic activity	Phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine = phosphate + UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine. HAMAP MF_00111
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00111
Subcellular location	Cytoplasm Probable HAMAP MF_00111.
Sequence similarities	Belongs to the EPSP synthase family. MurA subfamily.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	UDP-N-acetylgalactosamine biosynthetic process Inferred from electronic annotation. Source: InterPro cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	UDP-N-acetylglucosamine 1-carboxyvinyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 433	433	UDP-N-acetylglucosamine 1-carboxyvinyltransferase 2 HAMAP MF_00111		PRO_0000231203
Sites
	Active site	120	1	Proton donor By similarity
Amino acid modifications
	Modified residue	120	1	2-(S-cysteinyl)pyruvic acid O-phosphothioketal By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q831A8 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 90CA3F8B71E96FC3	FASTA	433	46,986
	10 20 30 40 50 60 MEQIIVHGGN TKLEGTVKIE GAKNAVLPIL AATLLAEEGV TTLKNVPILS DVFTMNQVIK 70 80 90 100 110 120 HLNVAIDFDE DANEVTIDAT QPLGIEANYE YVSKMRASIV VMGPLLARNG HAKVAMPGGC 130 140 150 160 170 180 AIGKRPIDLH LKGFQALGAK IIQKNGYIEA IADELIGNTI YLDFPSVGAT QNIMMAAVRA 190 200 210 220 230 240 KGTTIIENVA REPEIVDLAN ILNKMGANVI GAGTETMRIE GVDKLHAVEH SIVQDRIEAG 250 260 270 280 290 300 TFMVAAAMTE GNVLIEEAIS EHNRPLISKL TEMGAIIEEE ENGIRVIGPK HLKPTDVKTM 310 320 330 340 350 360 PHPGFPTDMQ AQMTAIQMFA EGTSIVTETV FENRYQHLEE MRRMNADLKI DGNIAVINGG 370 380 390 400 410 420 NELQGAAVEA TDLRAAAALI LVGLRANGIT RVSNLKYLDR GYYEFHKKLQ KLGANVERVN 430 DEKIEEKQAT TVI

References

[1]

"Role of mobile DNA in the evolution of vancomycin-resistant Enterococcus faecalis." Paulsen I.T., Banerjei L., Myers G.S.A., Nelson K.E., Seshadri R., Read T.D., Fouts D.E., Eisen J.A., Gill S.R., Heidelberg J.F., Tettelin H., Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J., Daugherty S.C., DeBoy R.T., Durkin S.A. , Kolonay J.F., Madupu R., Nelson W.C., Vamathevan J.J., Tran B., Upton J., Hansen T., Shetty J., Khouri H.M., Utterback T.R., Radune D., Ketchum K.A., Dougherty B.A., Fraser C.M. Science 299:2071-2074(2003) [PubMed: 12663927] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700802 / V583.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016830 Genomic DNA. Translation: AAO82314.1.
RefSeq	NP_816244.1. NC_004668.1.
3D structure databases
ProteinModelPortal	Q831A8.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1201464.
GenomeReviews	Gene locus EF_2605 in contig AE016830_GR.
KEGG	efa:EF2605.
NMPDR	fig|226185.1.peg.2429.
PATRIC	21855425. VBIEntFae7065_2432.
TIGR	EF_2605.
Phylogenomic databases
HOGENOM	HBG482701.
OMA	GYEHIED.
Enzyme and pathway databases
BioCyc	EFAE226185:EF_2605-MONOMER.
Family and domain databases
HAMAP	MF_00111. MurA. [Tree]
InterPro	IPR001986. Enolpyruvate_Tfrase_dom. IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b. IPR005750. UDP_GlcNAc_COvinyl_MurA. [Graphical view]
Gene3D	G3DSA:3.65.10.10. EPSP_synthase. 2 hits.
KO	K00790.
PANTHER	PTHR21090:SF4. AcGlu_Tran_MurA. 1 hit.
Pfam	PF00275. EPSP_synthase. 1 hit. [Graphical view]
SUPFAM	SSF55205. RNA3'_cycl/enolpyr_transf_A/B. 1 hit.
TIGRFAMs	TIGR01072. MurA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURA2_ENTFA

Accession

Primary (citable) accession number: Q831A8

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 4, 2006
Last sequence update:	June 1, 2003
Last modified:	January 25, 2012
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program