Reviewed, UniProtKB/Swiss-Prot Q830U3 (SPX_ENTFA)

Names and origin

Protein names

Recommended name:
    Regulatory protein spx

Gene names

Name:	spxA
Ordered Locus Names:	EF_2678

Organism

Enterococcus faecalis (Streptococcus faecalis) [Complete proteome] [HAMAP]

Taxonomic identifier

1351 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Enterococcaceae › Enterococcus

Protein attributes

Sequence length	132 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Interferes with activator-stimulated transcription by interaction with the RNA polymerase alpha-CTD. May function to globally reduce transcription of genes involved in growth- and development-promoting processes and to increase transcription of genes involved in thiol homeostasis, during periods of extreme stress By similarity.
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the arsC family. Spx subfamily.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Cytoplasm
Domain	Redox-active center
PTM	Disulfide bond
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	transcription regulator activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 132	132	Regulatory protein spx HAMAP MF_01132		PRO_0000162552
Amino acid modifications
	Disulfide bond	10 ↔ 13		Redox-active Potential

Sequences

	Sequence		Length	Mass (Da)
	Q830U3-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 152B87C19E7803A3	FASTA	132	15,510
	10 20 30 40 50 60 MLTLYTSPSC TSCRKARAWL QEHEIPFKER NIFSEPLNIE ELKAILIMTE DGTEEIISTR 70 80 90 100 110 120 SKVFQKLNMD LDELPLQDLL ELVQENPGLL RRPIMIDEKR LQVGFNEDEI RRFLPRDVRQ 130 LELRQAQLMA GL

References

[1]

"Role of mobile DNA in the evolution of vancomycin-resistant Enterococcus faecalis." Paulsen I.T., Banerjei L., Myers G.S.A., Nelson K.E., Seshadri R., Read T.D., Fouts D.E., Eisen J.A., Gill S.R., Heidelberg J.F., Tettelin H., Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J., Daugherty S.C., DeBoy R.T., Durkin S.A. , Kolonay J.F., Madupu R., Nelson W.C., Vamathevan J.J., Tran B., Upton J., Hansen T., Shetty J., Khouri H.M., Utterback T.R., Radune D., Ketchum K.A., Dougherty B.A., Fraser C.M. Science 299:2071-2074(2003) [PubMed: 12663927] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: V583 / ATCC 700802.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE016830 Genomic DNA. Translation: AAO82383.1.
RefSeq	NP_816313.1.
3D structure databases
SMR	Q830U3. Positions 1-118.
ModBase	Search...
Genome annotation databases
GeneID	1201534.
GenomeReviews	Gene locus EF_2678 in contig AE016830_GR.
KEGG	efa:EF2678.
NMPDR	fig|226185.1.peg.2498.
TIGR	EF_2678.
Phylogenomic databases
HOGENOM	Q830U3.
OMA	Q830U3. FTERNIF.
Enzyme and pathway databases
BioCyc	EFAE226185:EF_2678-MON.
Family and domain databases
HAMAP	MF_01132. [Tree]
InterPro	IPR006660. Arsenate_reductase-like. IPR006504. CHP1617_ArsC_rel. IPR012335. Thioredoxin_fold. [Graphical view]
Gene3D	G3DSA:3.40.30.10. Thioredoxin_fold. 1 hit.
Pfam	PF03960. ArsC. 1 hit. [Graphical view]
TIGRFAMs	TIGR01617. arsC_related. 1 hit.
PROSITE	PS51353. ARSC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SPX_ENTFA

Accession

Primary (citable) accession number: Q830U3

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 16, 2004
Last sequence update:	June 1, 2003
Last modified:	June 16, 2009
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)