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Q830U2 (SYW_ENTFA) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 68. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Tryptophan--tRNA ligase

EC=6.1.1.2
Alternative name(s):
Tryptophanyl-tRNA synthetase
Short name=TrpRS
Gene names
Name:trpS
Ordered Locus Names:EF_2679
OrganismEnterococcus faecalis (strain ATCC 700802 / V583) [Reference proteome] [HAMAP]
Taxonomic identifier226185 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesEnterococcaceaeEnterococcus

Protein attributes

Sequence length336 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp). HAMAP-Rule MF_00140

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_00140

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00140.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processtryptophanyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

tryptophan-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 336336Tryptophan--tRNA ligase HAMAP-Rule MF_00140
PRO_0000136631

Regions

Motif10 – 189"HIGH" region HAMAP-Rule MF_00140
Motif198 – 2025"KMSKS" region HAMAP-Rule MF_00140

Sites

Binding site2011ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q830U2 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: 67765FFCC966330A

FASTA33637,446
        10         20         30         40         50         60 
MKTIFSGIQP SGTPTIGNYI GAMKQFIELQ NEYNCYFCIV DEHAITVPQE PQKLRQQIRS 

        70         80         90        100        110        120 
LAALYLAVGL DPQKATIFIQ SEVSAHAEAG WIIQCNTSIG ELERMTQFKD KSQKNGRAGV 

       130        140        150        160        170        180 
SAGLLTYPPL MVGDIVLYNA DLVPVGDDQK QHLELTRDFV ERFNKRYAQK NQEILTIPEV 

       190        200        210        220        230        240 
KIAEQGSRIM SLQEPTKKMS KSDTNVKGFI SMLDEPAVIR KKIRSAVTDS TGVIEYNKEE 

       250        260        270        280        290        300 
KPGITNLLNI YSAATGQTVE ELVQAYEGKG YGDFKADLAE AVVALLEPIQ VRYQELLASE 

       310        320        330 
ELDMILDEGA ENARLVANKT LQRMKNAVGL GRKVRR 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE016830 Genomic DNA. Translation: AAO82384.1.
RefSeqNP_816314.1. NC_004668.1.

3D structure databases

ProteinModelPortalQ830U2.
SMRQ830U2. Positions 1-333.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING226185.EF2679.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAO82384; AAO82384; EF_2679.
GeneID1201535.
KEGGefa:EF2679.
PATRIC21855559. VBIEntFae7065_2499.

Phylogenomic databases

eggNOGCOG0180.
KOK01867.
OMAGWGQFKP.
OrthoDBEOG686NJQ.
ProtClustDBPRK00927.

Enzyme and pathway databases

BioCycEFAE226185:GHI1-2619-MONOMER.

Family and domain databases

Gene3D3.40.50.620. 1 hit.
HAMAPMF_00140_B. Trp_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002305. aa-tRNA-synth_Ic.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002306. Trp-tRNA-ligase.
IPR024109. Trp-tRNA-ligase_bac-type.
[Graphical view]
PANTHERPTHR10055. PTHR10055. 1 hit.
PfamPF00579. tRNA-synt_1b. 1 hit.
[Graphical view]
PRINTSPR01039. TRNASYNTHTRP.
TIGRFAMsTIGR00233. trpS. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYW_ENTFA
AccessionPrimary (citable) accession number: Q830U2
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 2003
Last sequence update: June 1, 2003
Last modified: February 19, 2014
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries