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Protein

Tryptophan--tRNA ligase

Gene

trpS

Organism
Enterococcus faecalis (strain ATCC 700802 / V583)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of tryptophan to tRNA(Trp).UniRule annotation

Catalytic activityi

ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp).UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei134L-tryptophanUniRule annotation1
Binding sitei189ATP; via amide nitrogen and carbonyl oxygenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi9 – 11ATPUniRule annotation3
Nucleotide bindingi17 – 18ATPUniRule annotation2
Nucleotide bindingi146 – 148ATPUniRule annotation3
Nucleotide bindingi198 – 202ATPUniRule annotation5

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEFAE226185:G1G0C-2619-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Tryptophan--tRNA ligaseUniRule annotation (EC:6.1.1.2UniRule annotation)
Alternative name(s):
Tryptophanyl-tRNA synthetaseUniRule annotation
Short name:
TrpRSUniRule annotation
Gene namesi
Name:trpSUniRule annotation
Ordered Locus Names:EF_2679
OrganismiEnterococcus faecalis (strain ATCC 700802 / V583)
Taxonomic identifieri226185 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesEnterococcaceaeEnterococcus
Proteomesi
  • UP000001415 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001366311 – 336Tryptophan--tRNA ligaseAdd BLAST336

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi226185.EF2679

Structurei

3D structure databases

ProteinModelPortaliQ830U2
SMRiQ830U2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi10 – 18"HIGH" regionUniRule annotation9
Motifi198 – 202"KMSKS" regionUniRule annotation5

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C31 Bacteria
COG0180 LUCA
KOiK01867
OMAiQRMTQFK

Family and domain databases

CDDicd00806 TrpRS_core, 1 hit
Gene3Di3.40.50.620, 2 hits
HAMAPiMF_00140_B Trp_tRNA_synth_B, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002305 aa-tRNA-synth_Ic
IPR014729 Rossmann-like_a/b/a_fold
IPR002306 Trp-tRNA-ligase
IPR024109 Trp-tRNA-ligase_bac-type
PfamiView protein in Pfam
PF00579 tRNA-synt_1b, 1 hit
PRINTSiPR01039 TRNASYNTHTRP
TIGRFAMsiTIGR00233 trpS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

Q830U2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKTIFSGIQP SGTPTIGNYI GAMKQFIELQ NEYNCYFCIV DEHAITVPQE
60 70 80 90 100
PQKLRQQIRS LAALYLAVGL DPQKATIFIQ SEVSAHAEAG WIIQCNTSIG
110 120 130 140 150
ELERMTQFKD KSQKNGRAGV SAGLLTYPPL MVGDIVLYNA DLVPVGDDQK
160 170 180 190 200
QHLELTRDFV ERFNKRYAQK NQEILTIPEV KIAEQGSRIM SLQEPTKKMS
210 220 230 240 250
KSDTNVKGFI SMLDEPAVIR KKIRSAVTDS TGVIEYNKEE KPGITNLLNI
260 270 280 290 300
YSAATGQTVE ELVQAYEGKG YGDFKADLAE AVVALLEPIQ VRYQELLASE
310 320 330
ELDMILDEGA ENARLVANKT LQRMKNAVGL GRKVRR
Length:336
Mass (Da):37,446
Last modified:June 1, 2003 - v1
Checksum:i67765FFCC966330A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016830 Genomic DNA Translation: AAO82384.1
RefSeqiNP_816314.1, NC_004668.1
WP_002378619.1, NZ_KE136528.1

Genome annotation databases

EnsemblBacteriaiAAO82384; AAO82384; EF_2679
GeneIDi1201535
KEGGiefa:EF2679
PATRICifig|226185.45.peg.883

Similar proteinsi

Entry informationi

Entry nameiSYW_ENTFA
AccessioniPrimary (citable) accession number: Q830U2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 2003
Last sequence update: June 1, 2003
Last modified: March 28, 2018
This is version 87 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health