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Q830K4 (THIM_ENTFA) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 72. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Hydroxyethylthiazole kinase

EC=2.7.1.50
Alternative name(s):
4-methyl-5-beta-hydroxyethylthiazole kinase
Short name=TH kinase
Short name=Thz kinase
Gene names
Name:thiM
Ordered Locus Names:EF_2777
OrganismEnterococcus faecalis (strain ATCC 700802 / V583) [Reference proteome] [HAMAP]
Taxonomic identifier226185 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesEnterococcaceaeEnterococcus

Protein attributes

Sequence length272 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole. HAMAP-Rule MF_00228

Cofactor

Magnesium By similarity. HAMAP-Rule MF_00228

Pathway

Cofactor biosynthesis; thiamine diphosphate biosynthesis; 4-methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-methylthiazole: step 1/1. HAMAP-Rule MF_00228

Sequence similarities

Belongs to the Thz kinase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 272272Hydroxyethylthiazole kinase HAMAP-Rule MF_00228
PRO_0000383858

Sites

Binding site441Substrate; via amide nitrogen By similarity
Binding site1191ATP By similarity
Binding site1721ATP By similarity
Binding site1991Substrate; via amide nitrogen By similarity

Secondary structure

.............................................. 272
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q830K4 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: 87AD9F1B009874F2

FASTA27229,719
        10         20         30         40         50         60 
MKTSVKFETI FPLTTAPLIQ CITNEITCES MANALLYIDA KPIMADDPRE FPQMFQQTSA 

        70         80         90        100        110        120 
LVLNLGHLSQ EREQSLLAAS DYARQVNKLT VVDLVGYGAS DIRNEVGEKL VHNQPTVVKG 

       130        140        150        160        170        180 
NLSEMRTFCQ LVSHGRGVDG SPLDQSEEAI EELIQALRQQ TQKFPQTVFL ATGIQDVLVS 

       190        200        210        220        230        240 
QEQVIVLQNG VPELDCFTGT GDLVGALVAA LLGEGNAPMT AAVAAVSYFN LCGEKAKTKS 

       250        260        270 
QGLADFRQNT LNQLSLLMKE KDWFEAVKGR VL 

« Hide

References

« Hide 'large scale' references
[1]"Role of mobile DNA in the evolution of vancomycin-resistant Enterococcus faecalis."
Paulsen I.T., Banerjei L., Myers G.S.A., Nelson K.E., Seshadri R., Read T.D., Fouts D.E., Eisen J.A., Gill S.R., Heidelberg J.F., Tettelin H., Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J., Daugherty S.C., DeBoy R.T., Durkin S.A. expand/collapse author list , Kolonay J.F., Madupu R., Nelson W.C., Vamathevan J.J., Tran B., Upton J., Hansen T., Shetty J., Khouri H.M., Utterback T.R., Radune D., Ketchum K.A., Dougherty B.A., Fraser C.M.
Science 299:2071-2074(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 700802 / V583.
[2]"Crystal structure of 4-methyl-5-(beta-hydroxyethyl)thiazole kinase (np_816404.1) from Enterococcus faecalis V583 at 2.57 A resolution."
Joint center for structural genomics (JCSG)
Submitted (FEB-2009) to the PDB data bank
Cited for: X-RAY CRYSTALLOGRAPHY (2.57 ANGSTROMS).
Strain: ATCC 700802 / V583.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE016830 Genomic DNA. Translation: AAO82474.1.
RefSeqNP_816404.1. NC_004668.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3DZVX-ray2.57A/B1-272[»]
ProteinModelPortalQ830K4.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING226185.EF2777.

Protocols and materials databases

DNASU1201627.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAO82474; AAO82474; EF_2777.
GeneID1201627.
KEGGefa:EF2777.
PATRIC21855737. VBIEntFae7065_2588.

Phylogenomic databases

eggNOGCOG2145.
KOK00878.
OMAAKPIMAE.
OrthoDBEOG628F8M.

Enzyme and pathway databases

BioCycEFAE226185:GHI1-2711-MONOMER.
UniPathwayUPA00060; UER00139.

Family and domain databases

Gene3D3.40.1190.20. 1 hit.
HAMAPMF_00228. Thz_kinase.
InterProIPR000417. Hyethyz_kinase.
IPR029056. Ribokinase-like.
[Graphical view]
PfamPF02110. HK. 1 hit.
[Graphical view]
PIRSFPIRSF000513. Thz_kinase. 1 hit.
PRINTSPR01099. HYETHTZKNASE.
SUPFAMSSF53613. SSF53613. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceQ830K4.

Entry information

Entry nameTHIM_ENTFA
AccessionPrimary (citable) accession number: Q830K4
Entry history
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: June 1, 2003
Last modified: June 11, 2014
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

PATHWAY comments

Index of metabolic and biosynthesis pathways