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Protein

Hydroxyethylthiazole kinase

Gene

thiM

Organism
Enterococcus faecalis (strain ATCC 700802 / V583)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the phosphorylation of the hydroxyl group of 4-methyl-5-beta-hydroxyethylthiazole (THZ).UniRule annotation

Catalytic activityi

ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathway:ithiamine diphosphate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 4-methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-methylthiazole.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Hydroxyethylthiazole kinase (thiM)
This subpathway is part of the pathway thiamine diphosphate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 4-methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-methylthiazole, the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei44 – 441Substrate; via amide nitrogenUniRule annotation
Binding sitei119 – 1191ATPUniRule annotation
Binding sitei172 – 1721ATPUniRule annotation
Binding sitei199 – 1991Substrate; via amide nitrogenUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Thiamine biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEFAE226185:GHI1-2711-MONOMER.
UniPathwayiUPA00060; UER00139.

Names & Taxonomyi

Protein namesi
Recommended name:
Hydroxyethylthiazole kinaseUniRule annotation (EC:2.7.1.50UniRule annotation)
Alternative name(s):
4-methyl-5-beta-hydroxyethylthiazole kinaseUniRule annotation
Short name:
TH kinaseUniRule annotation
Short name:
Thz kinaseUniRule annotation
Gene namesi
Name:thiMUniRule annotation
Ordered Locus Names:EF_2777
OrganismiEnterococcus faecalis (strain ATCC 700802 / V583)
Taxonomic identifieri226185 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesEnterococcaceaeEnterococcus
ProteomesiUP000001415 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 272272Hydroxyethylthiazole kinasePRO_0000383858Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi226185.EF2777.

Structurei

Secondary structure

1
272
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi7 – 93Combined sources
Beta strandi18 – 225Combined sources
Turni25 – 273Combined sources
Helixi28 – 3710Combined sources
Beta strandi41 – 433Combined sources
Helixi48 – 503Combined sources
Helixi51 – 555Combined sources
Beta strandi59 – 646Combined sources
Helixi70 – 8516Combined sources
Beta strandi90 – 934Combined sources
Turni95 – 984Combined sources
Helixi101 – 11212Combined sources
Beta strandi116 – 1216Combined sources
Helixi122 – 1287Combined sources
Helixi143 – 1453Combined sources
Helixi147 – 16317Combined sources
Beta strandi168 – 17912Combined sources
Beta strandi184 – 1874Combined sources
Helixi192 – 1954Combined sources
Helixi200 – 21415Combined sources
Helixi218 – 23922Combined sources
Helixi243 – 25614Combined sources
Helixi257 – 2593Combined sources
Helixi263 – 2664Combined sources
Beta strandi269 – 2724Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3DZVX-ray2.57A/B1-272[»]
ProteinModelPortaliQ830K4.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ830K4.

Family & Domainsi

Sequence similaritiesi

Belongs to the Thz kinase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG2145.
KOiK00878.
OMAiLPLMATH.
OrthoDBiEOG628F8M.

Family and domain databases

Gene3Di3.40.1190.20. 1 hit.
HAMAPiMF_00228. Thz_kinase.
InterProiIPR000417. Hyethyz_kinase.
IPR029056. Ribokinase-like.
[Graphical view]
PfamiPF02110. HK. 1 hit.
[Graphical view]
PIRSFiPIRSF000513. Thz_kinase. 1 hit.
PRINTSiPR01099. HYETHTZKNASE.
SUPFAMiSSF53613. SSF53613. 1 hit.

Sequencei

Sequence statusi: Complete.

Q830K4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKTSVKFETI FPLTTAPLIQ CITNEITCES MANALLYIDA KPIMADDPRE
60 70 80 90 100
FPQMFQQTSA LVLNLGHLSQ EREQSLLAAS DYARQVNKLT VVDLVGYGAS
110 120 130 140 150
DIRNEVGEKL VHNQPTVVKG NLSEMRTFCQ LVSHGRGVDG SPLDQSEEAI
160 170 180 190 200
EELIQALRQQ TQKFPQTVFL ATGIQDVLVS QEQVIVLQNG VPELDCFTGT
210 220 230 240 250
GDLVGALVAA LLGEGNAPMT AAVAAVSYFN LCGEKAKTKS QGLADFRQNT
260 270
LNQLSLLMKE KDWFEAVKGR VL
Length:272
Mass (Da):29,719
Last modified:June 1, 2003 - v1
Checksum:i87AD9F1B009874F2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016830 Genomic DNA. Translation: AAO82474.1.
RefSeqiNP_816404.1. NC_004668.1.
WP_002387209.1. NZ_KE136528.1.

Genome annotation databases

EnsemblBacteriaiAAO82474; AAO82474; EF_2777.
GeneIDi1201627.
KEGGiefa:EF2777.
PATRICi21855737. VBIEntFae7065_2588.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016830 Genomic DNA. Translation: AAO82474.1.
RefSeqiNP_816404.1. NC_004668.1.
WP_002387209.1. NZ_KE136528.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3DZVX-ray2.57A/B1-272[»]
ProteinModelPortaliQ830K4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi226185.EF2777.

Protocols and materials databases

DNASUi1201627.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAO82474; AAO82474; EF_2777.
GeneIDi1201627.
KEGGiefa:EF2777.
PATRICi21855737. VBIEntFae7065_2588.

Phylogenomic databases

eggNOGiCOG2145.
KOiK00878.
OMAiLPLMATH.
OrthoDBiEOG628F8M.

Enzyme and pathway databases

UniPathwayiUPA00060; UER00139.
BioCyciEFAE226185:GHI1-2711-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ830K4.

Family and domain databases

Gene3Di3.40.1190.20. 1 hit.
HAMAPiMF_00228. Thz_kinase.
InterProiIPR000417. Hyethyz_kinase.
IPR029056. Ribokinase-like.
[Graphical view]
PfamiPF02110. HK. 1 hit.
[Graphical view]
PIRSFiPIRSF000513. Thz_kinase. 1 hit.
PRINTSiPR01099. HYETHTZKNASE.
SUPFAMiSSF53613. SSF53613. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700802 / V583.
  2. "Crystal structure of 4-methyl-5-(beta-hydroxyethyl)thiazole kinase (np_816404.1) from Enterococcus faecalis V583 at 2.57 A resolution."
    Joint center for structural genomics (JCSG)
    Submitted (FEB-2009) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (2.57 ANGSTROMS).
    Strain: ATCC 700802 / V583.

Entry informationi

Entry nameiTHIM_ENTFA
AccessioniPrimary (citable) accession number: Q830K4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: June 1, 2003
Last modified: July 22, 2015
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.