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Protein

Hydroxyethylthiazole kinase

Gene

thiM

Organism
Enterococcus faecalis (strain ATCC 700802 / V583)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the phosphorylation of the hydroxyl group of 4-methyl-5-beta-hydroxyethylthiazole (THZ).UniRule annotation

Catalytic activityi

ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi: thiamine diphosphate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 4-methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-methylthiazole.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Hydroxyethylthiazole kinase (thiM)
This subpathway is part of the pathway thiamine diphosphate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 4-methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-methylthiazole, the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei44Substrate; via amide nitrogenUniRule annotation1
Binding sitei119ATPUniRule annotation1
Binding sitei172ATPUniRule annotation1
Binding sitei199Substrate; via amide nitrogenUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Thiamine biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00060; UER00139.

Names & Taxonomyi

Protein namesi
Recommended name:
Hydroxyethylthiazole kinaseUniRule annotation (EC:2.7.1.50UniRule annotation)
Alternative name(s):
4-methyl-5-beta-hydroxyethylthiazole kinaseUniRule annotation
Short name:
TH kinaseUniRule annotation
Short name:
Thz kinaseUniRule annotation
Gene namesi
Name:thiMUniRule annotation
Ordered Locus Names:EF_2777
OrganismiEnterococcus faecalis (strain ATCC 700802 / V583)
Taxonomic identifieri226185 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesEnterococcaceaeEnterococcus
Proteomesi
  • UP000001415 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003838581 – 272Hydroxyethylthiazole kinaseAdd BLAST272

Interactioni

Protein-protein interaction databases

STRINGi226185.EF2777.

Structurei

Secondary structure

1272
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi7 – 9Combined sources3
Beta strandi18 – 22Combined sources5
Turni25 – 27Combined sources3
Helixi28 – 37Combined sources10
Beta strandi41 – 43Combined sources3
Helixi48 – 50Combined sources3
Helixi51 – 55Combined sources5
Beta strandi59 – 64Combined sources6
Helixi70 – 85Combined sources16
Beta strandi90 – 93Combined sources4
Turni95 – 98Combined sources4
Helixi101 – 112Combined sources12
Beta strandi116 – 121Combined sources6
Helixi122 – 128Combined sources7
Helixi143 – 145Combined sources3
Helixi147 – 163Combined sources17
Beta strandi168 – 179Combined sources12
Beta strandi184 – 187Combined sources4
Helixi192 – 195Combined sources4
Helixi200 – 214Combined sources15
Helixi218 – 239Combined sources22
Helixi243 – 256Combined sources14
Helixi257 – 259Combined sources3
Helixi263 – 266Combined sources4
Beta strandi269 – 272Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3DZVX-ray2.57A/B1-272[»]
ProteinModelPortaliQ830K4.
SMRiQ830K4.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ830K4.

Family & Domainsi

Sequence similaritiesi

Belongs to the Thz kinase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C4U. Bacteria.
COG2145. LUCA.
KOiK00878.
OMAiSINDCAN.

Family and domain databases

CDDicd01170. THZ_kinase. 1 hit.
Gene3Di3.40.1190.20. 1 hit.
HAMAPiMF_00228. Thz_kinase. 1 hit.
InterProiIPR000417. Hyethyz_kinase.
IPR029056. Ribokinase-like.
[Graphical view]
PfamiPF02110. HK. 1 hit.
[Graphical view]
PIRSFiPIRSF000513. Thz_kinase. 1 hit.
PRINTSiPR01099. HYETHTZKNASE.
SUPFAMiSSF53613. SSF53613. 1 hit.

Sequencei

Sequence statusi: Complete.

Q830K4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKTSVKFETI FPLTTAPLIQ CITNEITCES MANALLYIDA KPIMADDPRE
60 70 80 90 100
FPQMFQQTSA LVLNLGHLSQ EREQSLLAAS DYARQVNKLT VVDLVGYGAS
110 120 130 140 150
DIRNEVGEKL VHNQPTVVKG NLSEMRTFCQ LVSHGRGVDG SPLDQSEEAI
160 170 180 190 200
EELIQALRQQ TQKFPQTVFL ATGIQDVLVS QEQVIVLQNG VPELDCFTGT
210 220 230 240 250
GDLVGALVAA LLGEGNAPMT AAVAAVSYFN LCGEKAKTKS QGLADFRQNT
260 270
LNQLSLLMKE KDWFEAVKGR VL
Length:272
Mass (Da):29,719
Last modified:June 1, 2003 - v1
Checksum:i87AD9F1B009874F2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016830 Genomic DNA. Translation: AAO82474.1.
RefSeqiNP_816404.1. NC_004668.1.
WP_002387209.1. NZ_KE136528.1.

Genome annotation databases

EnsemblBacteriaiAAO82474; AAO82474; EF_2777.
GeneIDi1201627.
KEGGiefa:EF2777.
PATRICi21855737. VBIEntFae7065_2588.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016830 Genomic DNA. Translation: AAO82474.1.
RefSeqiNP_816404.1. NC_004668.1.
WP_002387209.1. NZ_KE136528.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3DZVX-ray2.57A/B1-272[»]
ProteinModelPortaliQ830K4.
SMRiQ830K4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi226185.EF2777.

Protocols and materials databases

DNASUi1201627.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAO82474; AAO82474; EF_2777.
GeneIDi1201627.
KEGGiefa:EF2777.
PATRICi21855737. VBIEntFae7065_2588.

Phylogenomic databases

eggNOGiENOG4105C4U. Bacteria.
COG2145. LUCA.
KOiK00878.
OMAiSINDCAN.

Enzyme and pathway databases

UniPathwayiUPA00060; UER00139.

Miscellaneous databases

EvolutionaryTraceiQ830K4.

Family and domain databases

CDDicd01170. THZ_kinase. 1 hit.
Gene3Di3.40.1190.20. 1 hit.
HAMAPiMF_00228. Thz_kinase. 1 hit.
InterProiIPR000417. Hyethyz_kinase.
IPR029056. Ribokinase-like.
[Graphical view]
PfamiPF02110. HK. 1 hit.
[Graphical view]
PIRSFiPIRSF000513. Thz_kinase. 1 hit.
PRINTSiPR01099. HYETHTZKNASE.
SUPFAMiSSF53613. SSF53613. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTHIM_ENTFA
AccessioniPrimary (citable) accession number: Q830K4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: June 1, 2003
Last modified: November 2, 2016
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.