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Q830K4

- THIM_ENTFA

UniProt

Q830K4 - THIM_ENTFA

Protein

Hydroxyethylthiazole kinase

Gene

thiM

Organism
Enterococcus faecalis (strain ATCC 700802 / V583)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 73 (01 Oct 2014)
      Sequence version 1 (01 Jun 2003)
      Previous versions | rss
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    Functioni

    Catalyzes the phosphorylation of the hydroxyl group of 4-methyl-5-beta-hydroxyethylthiazole (THZ).UniRule annotation

    Catalytic activityi

    ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole.UniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei44 – 441Substrate; via amide nitrogenUniRule annotation
    Binding sitei119 – 1191ATPUniRule annotation
    Binding sitei172 – 1721ATPUniRule annotation
    Binding sitei199 – 1991Substrate; via amide nitrogenUniRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. hydroxyethylthiazole kinase activity Source: UniProtKB-HAMAP
    3. magnesium ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. thiamine biosynthetic process Source: UniProtKB-KW
    2. thiamine diphosphate biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Thiamine biosynthesis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciEFAE226185:GHI1-2711-MONOMER.
    UniPathwayiUPA00060; UER00139.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Hydroxyethylthiazole kinaseUniRule annotation (EC:2.7.1.50UniRule annotation)
    Alternative name(s):
    4-methyl-5-beta-hydroxyethylthiazole kinaseUniRule annotation
    Short name:
    TH kinaseUniRule annotation
    Short name:
    Thz kinaseUniRule annotation
    Gene namesi
    Name:thiMUniRule annotation
    Ordered Locus Names:EF_2777
    OrganismiEnterococcus faecalis (strain ATCC 700802 / V583)
    Taxonomic identifieri226185 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesEnterococcaceaeEnterococcus
    ProteomesiUP000001415: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 272272Hydroxyethylthiazole kinasePRO_0000383858Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi226185.EF2777.

    Structurei

    Secondary structure

    1
    272
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi7 – 93
    Beta strandi18 – 225
    Turni25 – 273
    Helixi28 – 3710
    Beta strandi41 – 433
    Helixi48 – 503
    Helixi51 – 555
    Beta strandi59 – 646
    Helixi70 – 8516
    Beta strandi90 – 934
    Turni95 – 984
    Helixi101 – 11212
    Beta strandi116 – 1216
    Helixi122 – 1287
    Helixi143 – 1453
    Helixi147 – 16317
    Beta strandi168 – 17912
    Beta strandi184 – 1874
    Helixi192 – 1954
    Helixi200 – 21415
    Helixi218 – 23922
    Helixi243 – 25614
    Helixi257 – 2593
    Helixi263 – 2664
    Beta strandi269 – 2724

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3DZVX-ray2.57A/B1-272[»]
    ProteinModelPortaliQ830K4.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ830K4.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the Thz kinase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG2145.
    KOiK00878.
    OMAiAKPIMAE.
    OrthoDBiEOG628F8M.

    Family and domain databases

    Gene3Di3.40.1190.20. 1 hit.
    HAMAPiMF_00228. Thz_kinase.
    InterProiIPR000417. Hyethyz_kinase.
    IPR029056. Ribokinase-like.
    [Graphical view]
    PfamiPF02110. HK. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000513. Thz_kinase. 1 hit.
    PRINTSiPR01099. HYETHTZKNASE.
    SUPFAMiSSF53613. SSF53613. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q830K4-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKTSVKFETI FPLTTAPLIQ CITNEITCES MANALLYIDA KPIMADDPRE    50
    FPQMFQQTSA LVLNLGHLSQ EREQSLLAAS DYARQVNKLT VVDLVGYGAS 100
    DIRNEVGEKL VHNQPTVVKG NLSEMRTFCQ LVSHGRGVDG SPLDQSEEAI 150
    EELIQALRQQ TQKFPQTVFL ATGIQDVLVS QEQVIVLQNG VPELDCFTGT 200
    GDLVGALVAA LLGEGNAPMT AAVAAVSYFN LCGEKAKTKS QGLADFRQNT 250
    LNQLSLLMKE KDWFEAVKGR VL 272
    Length:272
    Mass (Da):29,719
    Last modified:June 1, 2003 - v1
    Checksum:i87AD9F1B009874F2
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE016830 Genomic DNA. Translation: AAO82474.1.
    RefSeqiNP_816404.1. NC_004668.1.

    Genome annotation databases

    EnsemblBacteriaiAAO82474; AAO82474; EF_2777.
    GeneIDi1201627.
    KEGGiefa:EF2777.
    PATRICi21855737. VBIEntFae7065_2588.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE016830 Genomic DNA. Translation: AAO82474.1 .
    RefSeqi NP_816404.1. NC_004668.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3DZV X-ray 2.57 A/B 1-272 [» ]
    ProteinModelPortali Q830K4.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 226185.EF2777.

    Protocols and materials databases

    DNASUi 1201627.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAO82474 ; AAO82474 ; EF_2777 .
    GeneIDi 1201627.
    KEGGi efa:EF2777.
    PATRICi 21855737. VBIEntFae7065_2588.

    Phylogenomic databases

    eggNOGi COG2145.
    KOi K00878.
    OMAi AKPIMAE.
    OrthoDBi EOG628F8M.

    Enzyme and pathway databases

    UniPathwayi UPA00060 ; UER00139 .
    BioCyci EFAE226185:GHI1-2711-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei Q830K4.

    Family and domain databases

    Gene3Di 3.40.1190.20. 1 hit.
    HAMAPi MF_00228. Thz_kinase.
    InterProi IPR000417. Hyethyz_kinase.
    IPR029056. Ribokinase-like.
    [Graphical view ]
    Pfami PF02110. HK. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000513. Thz_kinase. 1 hit.
    PRINTSi PR01099. HYETHTZKNASE.
    SUPFAMi SSF53613. SSF53613. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 700802 / V583.
    2. "Crystal structure of 4-methyl-5-(beta-hydroxyethyl)thiazole kinase (np_816404.1) from Enterococcus faecalis V583 at 2.57 A resolution."
      Joint center for structural genomics (JCSG)
      Submitted (FEB-2009) to the PDB data bank
      Cited for: X-RAY CRYSTALLOGRAPHY (2.57 ANGSTROMS).
      Strain: ATCC 700802 / V583.

    Entry informationi

    Entry nameiTHIM_ENTFA
    AccessioniPrimary (citable) accession number: Q830K4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 22, 2009
    Last sequence update: June 1, 2003
    Last modified: October 1, 2014
    This is version 73 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3