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Q830K4

- THIM_ENTFA

UniProt

Q830K4 - THIM_ENTFA

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Protein

Hydroxyethylthiazole kinase

Gene

thiM

Organism
Enterococcus faecalis (strain ATCC 700802 / V583)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the phosphorylation of the hydroxyl group of 4-methyl-5-beta-hydroxyethylthiazole (THZ).UniRule annotation

Catalytic activityi

ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole.UniRule annotation

Cofactori

Magnesium.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei44 – 441Substrate; via amide nitrogenUniRule annotation
Binding sitei119 – 1191ATPUniRule annotation
Binding sitei172 – 1721ATPUniRule annotation
Binding sitei199 – 1991Substrate; via amide nitrogenUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. hydroxyethylthiazole kinase activity Source: UniProtKB-HAMAP
  3. magnesium ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. thiamine biosynthetic process Source: UniProtKB-KW
  2. thiamine diphosphate biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Thiamine biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEFAE226185:GHI1-2711-MONOMER.
UniPathwayiUPA00060; UER00139.

Names & Taxonomyi

Protein namesi
Recommended name:
Hydroxyethylthiazole kinaseUniRule annotation (EC:2.7.1.50UniRule annotation)
Alternative name(s):
4-methyl-5-beta-hydroxyethylthiazole kinaseUniRule annotation
Short name:
TH kinaseUniRule annotation
Short name:
Thz kinaseUniRule annotation
Gene namesi
Name:thiMUniRule annotation
Ordered Locus Names:EF_2777
OrganismiEnterococcus faecalis (strain ATCC 700802 / V583)
Taxonomic identifieri226185 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesEnterococcaceaeEnterococcus
ProteomesiUP000001415: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 272272Hydroxyethylthiazole kinasePRO_0000383858Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi226185.EF2777.

Structurei

Secondary structure

1
272
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi7 – 93
Beta strandi18 – 225
Turni25 – 273
Helixi28 – 3710
Beta strandi41 – 433
Helixi48 – 503
Helixi51 – 555
Beta strandi59 – 646
Helixi70 – 8516
Beta strandi90 – 934
Turni95 – 984
Helixi101 – 11212
Beta strandi116 – 1216
Helixi122 – 1287
Helixi143 – 1453
Helixi147 – 16317
Beta strandi168 – 17912
Beta strandi184 – 1874
Helixi192 – 1954
Helixi200 – 21415
Helixi218 – 23922
Helixi243 – 25614
Helixi257 – 2593
Helixi263 – 2664
Beta strandi269 – 2724

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3DZVX-ray2.57A/B1-272[»]
ProteinModelPortaliQ830K4.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ830K4.

Family & Domainsi

Sequence similaritiesi

Belongs to the Thz kinase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG2145.
KOiK00878.
OMAiAKPIMAE.
OrthoDBiEOG628F8M.

Family and domain databases

Gene3Di3.40.1190.20. 1 hit.
HAMAPiMF_00228. Thz_kinase.
InterProiIPR000417. Hyethyz_kinase.
IPR029056. Ribokinase-like.
[Graphical view]
PfamiPF02110. HK. 1 hit.
[Graphical view]
PIRSFiPIRSF000513. Thz_kinase. 1 hit.
PRINTSiPR01099. HYETHTZKNASE.
SUPFAMiSSF53613. SSF53613. 1 hit.

Sequencei

Sequence statusi: Complete.

Q830K4 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKTSVKFETI FPLTTAPLIQ CITNEITCES MANALLYIDA KPIMADDPRE
60 70 80 90 100
FPQMFQQTSA LVLNLGHLSQ EREQSLLAAS DYARQVNKLT VVDLVGYGAS
110 120 130 140 150
DIRNEVGEKL VHNQPTVVKG NLSEMRTFCQ LVSHGRGVDG SPLDQSEEAI
160 170 180 190 200
EELIQALRQQ TQKFPQTVFL ATGIQDVLVS QEQVIVLQNG VPELDCFTGT
210 220 230 240 250
GDLVGALVAA LLGEGNAPMT AAVAAVSYFN LCGEKAKTKS QGLADFRQNT
260 270
LNQLSLLMKE KDWFEAVKGR VL
Length:272
Mass (Da):29,719
Last modified:June 1, 2003 - v1
Checksum:i87AD9F1B009874F2
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE016830 Genomic DNA. Translation: AAO82474.1.
RefSeqiNP_816404.1. NC_004668.1.

Genome annotation databases

EnsemblBacteriaiAAO82474; AAO82474; EF_2777.
GeneIDi1201627.
KEGGiefa:EF2777.
PATRICi21855737. VBIEntFae7065_2588.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE016830 Genomic DNA. Translation: AAO82474.1 .
RefSeqi NP_816404.1. NC_004668.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3DZV X-ray 2.57 A/B 1-272 [» ]
ProteinModelPortali Q830K4.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 226185.EF2777.

Protocols and materials databases

DNASUi 1201627.
Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAO82474 ; AAO82474 ; EF_2777 .
GeneIDi 1201627.
KEGGi efa:EF2777.
PATRICi 21855737. VBIEntFae7065_2588.

Phylogenomic databases

eggNOGi COG2145.
KOi K00878.
OMAi AKPIMAE.
OrthoDBi EOG628F8M.

Enzyme and pathway databases

UniPathwayi UPA00060 ; UER00139 .
BioCyci EFAE226185:GHI1-2711-MONOMER.

Miscellaneous databases

EvolutionaryTracei Q830K4.

Family and domain databases

Gene3Di 3.40.1190.20. 1 hit.
HAMAPi MF_00228. Thz_kinase.
InterProi IPR000417. Hyethyz_kinase.
IPR029056. Ribokinase-like.
[Graphical view ]
Pfami PF02110. HK. 1 hit.
[Graphical view ]
PIRSFi PIRSF000513. Thz_kinase. 1 hit.
PRINTSi PR01099. HYETHTZKNASE.
SUPFAMi SSF53613. SSF53613. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700802 / V583.
  2. "Crystal structure of 4-methyl-5-(beta-hydroxyethyl)thiazole kinase (np_816404.1) from Enterococcus faecalis V583 at 2.57 A resolution."
    Joint center for structural genomics (JCSG)
    Submitted (FEB-2009) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (2.57 ANGSTROMS).
    Strain: ATCC 700802 / V583.

Entry informationi

Entry nameiTHIM_ENTFA
AccessioniPrimary (citable) accession number: Q830K4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: June 1, 2003
Last modified: October 1, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3