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Protein

Polyribonucleotide nucleotidyltransferase

Gene

pnp

Organism
Enterococcus faecalis (strain ATCC 700802 / V583)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction.UniRule annotation

Catalytic activityi

RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi490 – 4901MagnesiumUniRule annotation
Metal bindingi496 – 4961MagnesiumUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding, RNA-binding

Enzyme and pathway databases

BioCyciEFAE226185:GHI1-2992-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Polyribonucleotide nucleotidyltransferaseUniRule annotation (EC:2.7.7.8UniRule annotation)
Alternative name(s):
Polynucleotide phosphorylaseUniRule annotation
Short name:
PNPaseUniRule annotation
Gene namesi
Name:pnpUniRule annotation
Ordered Locus Names:EF_3064
OrganismiEnterococcus faecalis (strain ATCC 700802 / V583)
Taxonomic identifieri226185 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesEnterococcaceaeEnterococcus
ProteomesiUP000001415 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 704704Polyribonucleotide nucleotidyltransferasePRO_0000329636Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi226185.EF3064.

Structurei

3D structure databases

ProteinModelPortaliQ82ZJ2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini557 – 61660KHUniRule annotationAdd
BLAST
Domaini626 – 69469S1 motifUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the polyribonucleotide nucleotidyltransferase family.UniRule annotation
Contains 1 KH domain.UniRule annotation
Contains 1 S1 motif domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG1185.
KOiK00962.
OMAiRFMFHYN.
OrthoDBiEOG6WT8CC.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q82ZJ2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTEKQVFKTT WGGRPLEVEI GQLAKQANGA VLVRYGDTVV LSAAVASKEA
60 70 80 90 100
KDVDFFPLTV NYEEKMYAVG KIPGGFIKRE GRPSERATLT ARLIDRPIRP
110 120 130 140 150
MFSEGFRNEV QITNIVMSVE QDCTPEMAAM FGSSLALAIS DIPFDGPIAG
160 170 180 190 200
VDVGRINGEY VLNPTVEQAE QTDIELTVAG TKEAINMVES GAKEVSEEDM
210 220 230 240 250
LGALLFGFDA IKELVAFQEE IVAAVGKPKM DVDLLQVDAD LKKEIFDAYY
260 270 280 290 300
NTMKTAVMTE EKLAREVEID KVKDTVKEVY AEKFSEHEEE AQLLKEVKQI
310 320 330 340 350
AEDLEKDVVR ELITIDKIRP DGRKLDEIRH LSSEVSILPR VHGSGLFTRG
360 370 380 390 400
QTQALSVCTL APLGEHQIID GLGVQDSKRF IHHYNFPQFS VGSTGRAGSP
410 420 430 440 450
GRREIGHGAL GERALAQIIP SEEDFPYTIR LVAEVLESNG SSSQASICAG
460 470 480 490 500
TLALMDAGVP IKAPVAGIAM GLVSDGENYT ILTDIQGLED HLGDMDFKVA
510 520 530 540 550
GTKDGITALQ MDIKIQGITE QILTEALDQA KKARMEILEE LTTTIAAPRE
560 570 580 590 600
ELSQYAPKIE MIQIKPAKIK DVIGKGGETI NSIIDETGVK IDIDQDGNVS
610 620 630 640 650
IASSDAEMIK KAIKIIEELT KEVEVGQVYL AKVVRIEKFG AFVNLIKGKD
660 670 680 690 700
GLIHISQLAN ERVNNVEDVV KLGDEVLVKV TEIDKQGRVN VSRKALLNEE

NKEK
Length:704
Mass (Da):77,048
Last modified:June 1, 2003 - v1
Checksum:i51148FB2AACC1225
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016830 Genomic DNA. Translation: AAO82746.1.
RefSeqiNP_816676.1. NC_004668.1.
WP_002378800.1. NZ_KE136524.1.

Genome annotation databases

EnsemblBacteriaiAAO82746; AAO82746; EF_3064.
GeneIDi1201910.
KEGGiefa:EF3064.
PATRICi21856289. VBIEntFae7065_2857.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016830 Genomic DNA. Translation: AAO82746.1.
RefSeqiNP_816676.1. NC_004668.1.
WP_002378800.1. NZ_KE136524.1.

3D structure databases

ProteinModelPortaliQ82ZJ2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi226185.EF3064.

Protocols and materials databases

DNASUi1201910.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAO82746; AAO82746; EF_3064.
GeneIDi1201910.
KEGGiefa:EF3064.
PATRICi21856289. VBIEntFae7065_2857.

Phylogenomic databases

eggNOGiCOG1185.
KOiK00962.
OMAiRFMFHYN.
OrthoDBiEOG6WT8CC.

Enzyme and pathway databases

BioCyciEFAE226185:GHI1-2992-MONOMER.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700802 / V583.

Entry informationi

Entry nameiPNP_ENTFA
AccessioniPrimary (citable) accession number: Q82ZJ2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: June 1, 2003
Last modified: May 27, 2015
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.