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Q82ZJ0

- DEF_ENTFA

UniProt

Q82ZJ0 - DEF_ENTFA

Protein

Peptide deformylase

Gene

def

Organism
Enterococcus faecalis (strain ATCC 700802 / V583)
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 74 (01 Oct 2014)
      Sequence version 1 (01 Jun 2003)
      Previous versions | rss
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    Functioni

    Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions.UniRule annotation

    Catalytic activityi

    Formyl-L-methionyl peptide + H2O = formate + methionyl peptide.UniRule annotation

    Cofactori

    Binds 1 Fe2+ ion.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi114 – 1141IronUniRule annotation
    Metal bindingi157 – 1571IronUniRule annotation
    Active sitei158 – 1581UniRule annotation
    Metal bindingi161 – 1611IronUniRule annotation

    GO - Molecular functioni

    1. iron ion binding Source: InterPro
    2. peptide deformylase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. translation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    Iron, Metal-binding

    Enzyme and pathway databases

    BioCyciEFAE226185:GHI1-2994-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Peptide deformylaseUniRule annotation (EC:3.5.1.88UniRule annotation)
    Short name:
    PDFUniRule annotation
    Alternative name(s):
    Polypeptide deformylaseUniRule annotation
    Gene namesi
    Name:defUniRule annotation
    Ordered Locus Names:EF_3066
    OrganismiEnterococcus faecalis (strain ATCC 700802 / V583)
    Taxonomic identifieri226185 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesEnterococcaceaeEnterococcus
    ProteomesiUP000001415: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 187187Peptide deformylasePRO_0000082782Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi226185.EF3066.

    Structurei

    Secondary structure

    1
    187
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi4 – 63
    Helixi13 – 164
    Helixi28 – 4518
    Helixi47 – 537
    Beta strandi59 – 624
    Helixi63 – 664
    Beta strandi70 – 778
    Beta strandi89 – 10113
    Beta strandi105 – 1084
    Beta strandi127 – 13610
    Beta strandi142 – 1487
    Helixi149 – 16214
    Helixi167 – 1704
    Beta strandi173 – 1753
    Beta strandi184 – 1863

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2OS0X-ray1.30A1-187[»]
    2OS1X-ray1.50A1-187[»]
    ProteinModelPortaliQ82ZJ0.
    SMRiQ82ZJ0. Positions 1-187.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ82ZJ0.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the polypeptide deformylase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0242.
    KOiK01462.
    OMAiNDPENET.
    OrthoDBiEOG6PZXGQ.

    Family and domain databases

    Gene3Di3.90.45.10. 1 hit.
    HAMAPiMF_00163. Pep_deformylase.
    InterProiIPR000181. Fmet_deformylase.
    IPR023635. Peptide_deformylase.
    [Graphical view]
    PANTHERiPTHR10458. PTHR10458. 1 hit.
    PfamiPF01327. Pep_deformylase. 1 hit.
    [Graphical view]
    PIRSFiPIRSF004749. Pep_def. 1 hit.
    PRINTSiPR01576. PDEFORMYLASE.
    SUPFAMiSSF56420. SSF56420. 1 hit.
    TIGRFAMsiTIGR00079. pept_deformyl. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q82ZJ0-1 [UniParc]FASTAAdd to Basket

    « Hide

    MITMKDIIRE GNPTLRAVAE EVPVPITEED RQLGEDMLTF LKNSQDPVKA    50
    EELQLRGGVG LAAPQLDISK RIIAVHVPSN DPENETPSLS TVMYNPKILS 100
    HSVQDVCLGE GEGCLSVDRD VPGYVVRHNK ITVSYFDMAG EKHKVRLKNY 150
    EAIVVQHEID HINGIMFYDH INKENPFALK EGVLVIE 187
    Length:187
    Mass (Da):20,912
    Last modified:June 1, 2003 - v1
    Checksum:i9CAF46335311B0B2
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE016830 Genomic DNA. Translation: AAO82748.1.
    RefSeqiNP_816678.1. NC_004668.1.

    Genome annotation databases

    EnsemblBacteriaiAAO82748; AAO82748; EF_3066.
    GeneIDi1201912.
    KEGGiefa:EF3066.
    PATRICi21856293. VBIEntFae7065_2859.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE016830 Genomic DNA. Translation: AAO82748.1 .
    RefSeqi NP_816678.1. NC_004668.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2OS0 X-ray 1.30 A 1-187 [» ]
    2OS1 X-ray 1.50 A 1-187 [» ]
    ProteinModelPortali Q82ZJ0.
    SMRi Q82ZJ0. Positions 1-187.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 226185.EF3066.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAO82748 ; AAO82748 ; EF_3066 .
    GeneIDi 1201912.
    KEGGi efa:EF3066.
    PATRICi 21856293. VBIEntFae7065_2859.

    Phylogenomic databases

    eggNOGi COG0242.
    KOi K01462.
    OMAi NDPENET.
    OrthoDBi EOG6PZXGQ.

    Enzyme and pathway databases

    BioCyci EFAE226185:GHI1-2994-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei Q82ZJ0.

    Family and domain databases

    Gene3Di 3.90.45.10. 1 hit.
    HAMAPi MF_00163. Pep_deformylase.
    InterProi IPR000181. Fmet_deformylase.
    IPR023635. Peptide_deformylase.
    [Graphical view ]
    PANTHERi PTHR10458. PTHR10458. 1 hit.
    Pfami PF01327. Pep_deformylase. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF004749. Pep_def. 1 hit.
    PRINTSi PR01576. PDEFORMYLASE.
    SUPFAMi SSF56420. SSF56420. 1 hit.
    TIGRFAMsi TIGR00079. pept_deformyl. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 700802 / V583.

    Entry informationi

    Entry nameiDEF_ENTFA
    AccessioniPrimary (citable) accession number: Q82ZJ0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 15, 2003
    Last sequence update: June 1, 2003
    Last modified: October 1, 2014
    This is version 74 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3