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Q82ZJ0 (DEF_ENTFA) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 58. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Peptide deformylase
Short name=PDF
EC=3.5.1.88
Alternative name(s):
Polypeptide deformylase
Gene names
Name:def
Ordered Locus Names:EF_3066
OrganismEnterococcus faecalis (Streptococcus faecalis)
Taxonomic identifier1351 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Lactobacillales › Enterococcaceae › Enterococcus

Protein attributes

Sequence length187 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions By similarity. HAMAP MF_00163

Catalytic activity

Formyl-L-methionyl peptide + H2O = formate + methionyl peptide. HAMAP MF_00163

Cofactor

Binds 1 Fe2+ ion By similarity. HAMAP MF_00163

Sequence similarities

Belongs to the polypeptide deformylase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   LigandIron
Metal-binding
   Molecular functionHydrolase
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological processtranslation

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular functioniron ion binding

Inferred from electronic annotation. Source: InterPro

peptide deformylase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 187187Peptide deformylase HAMAP MF_00163
PRO_0000082782

Sites

Active site1581 By similarity
Metal binding1141Iron By similarity
Metal binding1571Iron By similarity
Metal binding1611Iron By similarity

Secondary structure

1 ............................. 187
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q82ZJ0 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: 9CAF46335311B0B2

FASTA18720,912
        10         20         30         40         50         60 
MITMKDIIRE GNPTLRAVAE EVPVPITEED RQLGEDMLTF LKNSQDPVKA EELQLRGGVG 

        70         80         90        100        110        120 
LAAPQLDISK RIIAVHVPSN DPENETPSLS TVMYNPKILS HSVQDVCLGE GEGCLSVDRD 

       130        140        150        160        170        180 
VPGYVVRHNK ITVSYFDMAG EKHKVRLKNY EAIVVQHEID HINGIMFYDH INKENPFALK 


EGVLVIE

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE016830 Genomic DNA. Translation: AAO82748.1.
RefSeqNP_816678.1. NC_004668.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2OS0X-ray1.30A1-187[»]
2OS1X-ray1.50A1-187[»]
ProteinModelPortalQ82ZJ0.
SMRQ82ZJ0. Positions 1-187.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID1201912.
GenomeReviewsGene locus EF_3066 in contig AE016830_GR.
KEGGefa:EF3066.
NMPDRfig|226185.1.peg.2863.
PATRIC21856293. VBIEntFae7065_2859.
TIGREF_3066.

Phylogenomic databases

HOGENOMHBG665227.
OMALKNGEGC.
ProtClustDBPRK00150.

Enzyme and pathway databases

BioCycEFAE226185:EF_3066-MONOMER.

Family and domain databases

HAMAPMF_00163. Pep_deformylase.
[Tree]
InterProIPR000181. Fmet_deformylase.
IPR023635. Peptide_deformylase.
[Graphical view]
Gene3DG3DSA:3.90.45.10. Fmet_deformylase. 1 hit.
KOK01462.
PANTHERPTHR10458. Fmet_deformylase. 1 hit.
PfamPF01327. Pep_deformylase. 1 hit.
[Graphical view]
PIRSFPIRSF004749. Pep_def. 1 hit.
PRINTSPR01576. PDEFORMYLASE.
SUPFAMSSF56420. Fmet_deformylase. 1 hit.
TIGRFAMsTIGR00079. Pept_deformyl. 1 hit.
ProtoNetSearch...

Entry information

Entry nameDEF_ENTFA
AccessionPrimary (citable) accession number: Q82ZJ0
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 2003
Last sequence update: June 1, 2003
Last modified: January 25, 2012
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

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