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Q82ZJ0

- DEF_ENTFA

UniProt

Q82ZJ0 - DEF_ENTFA

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Protein

Peptide deformylase

Gene

def

Organism
Enterococcus faecalis (strain ATCC 700802 / V583)
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli

Functioni

Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions.UniRule annotation

Catalytic activityi

Formyl-L-methionyl peptide + H2O = formate + methionyl peptide.UniRule annotation

Cofactori

Binds 1 Fe2+ ion.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi114 – 1141IronUniRule annotation
Metal bindingi157 – 1571IronUniRule annotation
Active sitei158 – 1581UniRule annotation
Metal bindingi161 – 1611IronUniRule annotation

GO - Molecular functioni

  1. iron ion binding Source: InterPro
  2. peptide deformylase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. translation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

BioCyciEFAE226185:GHI1-2994-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Peptide deformylaseUniRule annotation (EC:3.5.1.88UniRule annotation)
Short name:
PDFUniRule annotation
Alternative name(s):
Polypeptide deformylaseUniRule annotation
Gene namesi
Name:defUniRule annotation
Ordered Locus Names:EF_3066
OrganismiEnterococcus faecalis (strain ATCC 700802 / V583)
Taxonomic identifieri226185 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesEnterococcaceaeEnterococcus
ProteomesiUP000001415: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 187187Peptide deformylasePRO_0000082782Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi226185.EF3066.

Structurei

Secondary structure

1
187
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi4 – 63
Helixi13 – 164
Helixi28 – 4518
Helixi47 – 537
Beta strandi59 – 624
Helixi63 – 664
Beta strandi70 – 778
Beta strandi89 – 10113
Beta strandi105 – 1084
Beta strandi127 – 13610
Beta strandi142 – 1487
Helixi149 – 16214
Helixi167 – 1704
Beta strandi173 – 1753
Beta strandi184 – 1863

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2OS0X-ray1.30A1-187[»]
2OS1X-ray1.50A1-187[»]
ProteinModelPortaliQ82ZJ0.
SMRiQ82ZJ0. Positions 1-187.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ82ZJ0.

Family & Domainsi

Sequence similaritiesi

Belongs to the polypeptide deformylase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0242.
KOiK01462.
OMAiNDPENET.
OrthoDBiEOG6PZXGQ.

Family and domain databases

Gene3Di3.90.45.10. 1 hit.
HAMAPiMF_00163. Pep_deformylase.
InterProiIPR000181. Fmet_deformylase.
IPR023635. Peptide_deformylase.
[Graphical view]
PANTHERiPTHR10458. PTHR10458. 1 hit.
PfamiPF01327. Pep_deformylase. 1 hit.
[Graphical view]
PIRSFiPIRSF004749. Pep_def. 1 hit.
PRINTSiPR01576. PDEFORMYLASE.
SUPFAMiSSF56420. SSF56420. 1 hit.
TIGRFAMsiTIGR00079. pept_deformyl. 1 hit.

Sequencei

Sequence statusi: Complete.

Q82ZJ0-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MITMKDIIRE GNPTLRAVAE EVPVPITEED RQLGEDMLTF LKNSQDPVKA
60 70 80 90 100
EELQLRGGVG LAAPQLDISK RIIAVHVPSN DPENETPSLS TVMYNPKILS
110 120 130 140 150
HSVQDVCLGE GEGCLSVDRD VPGYVVRHNK ITVSYFDMAG EKHKVRLKNY
160 170 180
EAIVVQHEID HINGIMFYDH INKENPFALK EGVLVIE
Length:187
Mass (Da):20,912
Last modified:June 1, 2003 - v1
Checksum:i9CAF46335311B0B2
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE016830 Genomic DNA. Translation: AAO82748.1.
RefSeqiNP_816678.1. NC_004668.1.

Genome annotation databases

EnsemblBacteriaiAAO82748; AAO82748; EF_3066.
GeneIDi1201912.
KEGGiefa:EF3066.
PATRICi21856293. VBIEntFae7065_2859.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE016830 Genomic DNA. Translation: AAO82748.1 .
RefSeqi NP_816678.1. NC_004668.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2OS0 X-ray 1.30 A 1-187 [» ]
2OS1 X-ray 1.50 A 1-187 [» ]
ProteinModelPortali Q82ZJ0.
SMRi Q82ZJ0. Positions 1-187.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 226185.EF3066.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAO82748 ; AAO82748 ; EF_3066 .
GeneIDi 1201912.
KEGGi efa:EF3066.
PATRICi 21856293. VBIEntFae7065_2859.

Phylogenomic databases

eggNOGi COG0242.
KOi K01462.
OMAi NDPENET.
OrthoDBi EOG6PZXGQ.

Enzyme and pathway databases

BioCyci EFAE226185:GHI1-2994-MONOMER.

Miscellaneous databases

EvolutionaryTracei Q82ZJ0.

Family and domain databases

Gene3Di 3.90.45.10. 1 hit.
HAMAPi MF_00163. Pep_deformylase.
InterProi IPR000181. Fmet_deformylase.
IPR023635. Peptide_deformylase.
[Graphical view ]
PANTHERi PTHR10458. PTHR10458. 1 hit.
Pfami PF01327. Pep_deformylase. 1 hit.
[Graphical view ]
PIRSFi PIRSF004749. Pep_def. 1 hit.
PRINTSi PR01576. PDEFORMYLASE.
SUPFAMi SSF56420. SSF56420. 1 hit.
TIGRFAMsi TIGR00079. pept_deformyl. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700802 / V583.

Entry informationi

Entry nameiDEF_ENTFA
AccessioniPrimary (citable) accession number: Q82ZJ0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 2003
Last sequence update: June 1, 2003
Last modified: October 1, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3