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Protein

Glutathione biosynthesis bifunctional protein GshAB

Gene

gshAB

Organism
Enterococcus faecalis (strain ATCC 700802 / V583)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Synthesizes glutathione from L-glutamate and L-cysteine via gamma-L-glutamyl-L-cysteine.UniRule annotation

Catalytic activityi

ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine.UniRule annotation
ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione.UniRule annotation

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 2 magnesium or manganese ions per subunit.By similarity

Pathwayi: glutathione biosynthesis

This protein is involved in step 1 and 2 of the subpathway that synthesizes glutathione from L-cysteine and L-glutamate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Glutathione biosynthesis bifunctional protein GshAB (gshAB)
  2. Glutathione biosynthesis bifunctional protein GshAB (gshAB)
This subpathway is part of the pathway glutathione biosynthesis, which is itself part of Sulfur metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes glutathione from L-cysteine and L-glutamate, the pathway glutathione biosynthesis and in Sulfur metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi700Magnesium or manganese 1UniRule annotation1
Metal bindingi721Magnesium or manganese 1UniRule annotation1
Metal bindingi721Magnesium or manganese 2UniRule annotation1
Metal bindingi723Magnesium or manganese 2UniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi520 – 578ATPUniRule annotationAdd BLAST59

GO - Molecular functioni

Keywordsi

Molecular functionLigase, Multifunctional enzyme
Biological processGlutathione biosynthesis
LigandATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEFAE226185:G1G0C-3016-MONOMER
UniPathwayiUPA00142; UER00209
UPA00142; UER00210

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione biosynthesis bifunctional protein GshABUniRule annotation
Alternative name(s):
Gamma-GCS-GSUniRule annotation
Short name:
GCS-GSUniRule annotation
Including the following 2 domains:
Glutamate--cysteine ligaseUniRule annotation (EC:6.3.2.2UniRule annotation)
Alternative name(s):
Gamma-ECSUniRule annotation
Short name:
GCSUniRule annotation
Gamma-glutamylcysteine synthetaseUniRule annotation
Glutathione synthetaseUniRule annotation (EC:6.3.2.3UniRule annotation)
Alternative name(s):
GSH synthetaseUniRule annotation
Short name:
GSUniRule annotation
Short name:
GSH-SUniRule annotation
Short name:
GSHaseUniRule annotation
Glutathione synthaseUniRule annotation
Gene namesi
Name:gshABUniRule annotation
Synonyms:gshFUniRule annotation
Ordered Locus Names:EF_3089
OrganismiEnterococcus faecalis (strain ATCC 700802 / V583)
Taxonomic identifieri226185 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesEnterococcaceaeEnterococcus
Proteomesi
  • UP000001415 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001925511 – 756Glutathione biosynthesis bifunctional protein GshABAdd BLAST756

Proteomic databases

PRIDEiQ82ZG8

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi226185.EF3089

Structurei

3D structure databases

ProteinModelPortaliQ82ZG8
SMRiQ82ZG8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini493 – 751ATP-graspUniRule annotationAdd BLAST259

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 338Glutamate--cysteine ligaseAdd BLAST338

Sequence similaritiesi

In the N-terminal section; belongs to the glutamate--cysteine ligase type 1 family. Type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105D9M Bacteria
COG1181 LUCA
COG2918 LUCA
KOiK01919
OMAiEANFNPM

Family and domain databases

Gene3Di3.30.1490.20, 1 hit
HAMAPiMF_00782 Glut_biosynth, 1 hit
InterProiView protein in InterPro
IPR011761 ATP-grasp
IPR003806 ATP-grasp_DUF201-type
IPR013815 ATP_grasp_subdomain_1
IPR014746 Gln_synth/guanido_kin_cat_dom
IPR007370 Glu_cys_ligase
IPR006335 Glut_biosynth
IPR006334 Glut_cys_ligase
PANTHERiPTHR38761 PTHR38761, 2 hits
PfamiView protein in Pfam
PF02655 ATP-grasp_3, 1 hit
PF04262 Glu_cys_ligase, 2 hits
SUPFAMiSSF55931 SSF55931, 1 hit
TIGRFAMsiTIGR01435 glu_cys_lig_rel, 1 hit
PROSITEiView protein in PROSITE
PS50975 ATP_GRASP, 1 hit

Sequencei

Sequence statusi: Complete.

Q82ZG8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNYRELMQKK NVRPYVLMAR FGLEKENQRS TREGLLATTE HPTVFGNRSY
60 70 80 90 100
HPYIQTDFSE TQLELITPVA NSGTEMLRFL DAIHDVARRS IPEDEMLWPL
110 120 130 140 150
SMPPQLPTKD EEIKIAKLDQ YDAVLYRRYL AKEYGKRKQM VSGIHFNFEY
160 170 180 190 200
DQALIQQLYD EQSEVTDCKQ FKTKVYMKVA RNFLRYRWLI TYLFGASPVS
210 220 230 240 250
EDRYFRVYDD QPQEPVRSIR NSTYGYRNHD NVKVSYASLE RYLEDIHRMV
260 270 280 290 300
ENGLLSEEKE FYAPVRLRGG KQMSDLPKTG IRYIELRNLD LNPFSRLGIV
310 320 330 340 350
EDTVDFLHYF MLYLLWTDEK EEADEWVKTG DIFNEQVALG HPHETIKLIA
360 370 380 390 400
EGDRIFSEMI DMLDALGIRK GKEVVGKYYQ QLRNPQDTVS GKMWTIIQEN
410 420 430 440 450
SNSELGNIFG NQYQSMAFER PYQLAGFREM ELSTQIFLFD AIQKGLEIEI
460 470 480 490 500
LDEQEQFLKL QHGEHIEYVK NANMTSKDNY VVPLIMENKT VTKKILSAAG
510 520 530 540 550
FHVPGGEEFS SFIEAQEAHL RYANKAFVVK PKSTNYGLGI TIFKEGASLE
560 570 580 590 600
DFTEALRIAF KEDTAVLIEE FLPGTEYRFF VLDNDVKAIM LRVPANVTGD
610 620 630 640 650
GKHTVEELVA AKNSDPLRGT NHRAPLELIQ LNDLEKLMLK EQGLTIYSVP
660 670 680 690 700
EKEQIVYLRE NSNVSTGGDS IDMTDVIDDS YKQIAIEAVA ALGAKICGID
710 720 730 740 750
LIIPDKDVKG TRDSLTYGII EANFNPAMHM HVYPYAGQGR RLTMDVLKLL

YPEVVQ
Length:756
Mass (Da):87,212
Last modified:June 1, 2003 - v1
Checksum:iD282F6561A9D07A4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016830 Genomic DNA Translation: AAO82770.1
RefSeqiNP_816700.1, NC_004668.1
WP_002399785.1, NZ_KE136524.1

Genome annotation databases

EnsemblBacteriaiAAO82770; AAO82770; EF_3089
GeneIDi1201934
KEGGiefa:EF3089
PATRICifig|226185.45.peg.483

Similar proteinsi

Entry informationi

Entry nameiGSHAB_ENTFA
AccessioniPrimary (citable) accession number: Q82ZG8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 24, 2004
Last sequence update: June 1, 2003
Last modified: May 23, 2018
This is version 99 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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