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Q82ZG8

- GSHAB_ENTFA

UniProt

Q82ZG8 - GSHAB_ENTFA

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Protein

Glutathione biosynthesis bifunctional protein GshAB

Gene

gshAB

Organism
Enterococcus faecalis (strain ATCC 700802 / V583)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

Synthesizes glutathione from L-glutamate and L-cysteine via gamma-L-glutamyl-L-cysteine.UniRule annotation

Catalytic activityi

ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine.UniRule annotation
ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione.UniRule annotation

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 2 magnesium or manganese ions per subunit.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi700 – 7001Magnesium or manganese 1UniRule annotation
Metal bindingi721 – 7211Magnesium or manganese 1UniRule annotation
Metal bindingi721 – 7211Magnesium or manganese 2UniRule annotation
Metal bindingi723 – 7231Magnesium or manganese 2UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi520 – 57859ATPUniRule annotationAdd
BLAST

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. glutamate-cysteine ligase activity Source: UniProtKB-HAMAP
  3. glutathione synthase activity Source: UniProtKB-HAMAP
  4. magnesium ion binding Source: UniProtKB-HAMAP
  5. manganese ion binding Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Glutathione biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEFAE226185:GHI1-3016-MONOMER.
UniPathwayiUPA00142; UER00209.
UPA00142; UER00210.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione biosynthesis bifunctional protein GshABUniRule annotation
Alternative name(s):
Gamma-GCS-GSUniRule annotation
Short name:
GCS-GSUniRule annotation
Including the following 2 domains:
Glutamate--cysteine ligaseUniRule annotation (EC:6.3.2.2UniRule annotation)
Alternative name(s):
Gamma-ECSUniRule annotation
Short name:
GCSUniRule annotation
Gamma-glutamylcysteine synthetaseUniRule annotation
Glutathione synthetaseUniRule annotation (EC:6.3.2.3UniRule annotation)
Alternative name(s):
GSH synthetaseUniRule annotation
Short name:
GSUniRule annotation
Short name:
GSH-SUniRule annotation
Short name:
GSHaseUniRule annotation
Glutathione synthaseUniRule annotation
Gene namesi
Name:gshABUniRule annotation
Synonyms:gshFUniRule annotation
Ordered Locus Names:EF_3089
OrganismiEnterococcus faecalis (strain ATCC 700802 / V583)
Taxonomic identifieri226185 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesEnterococcaceaeEnterococcus
ProteomesiUP000001415: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 756756Glutathione biosynthesis bifunctional protein GshABPRO_0000192551Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi226185.EF3089.

Structurei

3D structure databases

ProteinModelPortaliQ82ZG8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini493 – 751259ATP-graspUniRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 338338Glutamate--cysteine ligaseAdd
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the glutamate--cysteine ligase type 1 family. Type 2 subfamily.UniRule annotation
Contains 1 ATP-grasp domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG1181.
KOiK01919.
OMAiRYLAKEY.
OrthoDBiEOG6BKJ7H.

Family and domain databases

Gene3Di3.30.1490.20. 1 hit.
3.30.470.20. 3 hits.
HAMAPiMF_00782. Glut_biosynth.
InterProiIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR007370. Glu_cys_ligase.
IPR006335. Glut_biosynth.
[Graphical view]
PfamiPF04262. Glu_cys_ligase. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01435. glu_cys_lig_rel. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q82ZG8-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNYRELMQKK NVRPYVLMAR FGLEKENQRS TREGLLATTE HPTVFGNRSY
60 70 80 90 100
HPYIQTDFSE TQLELITPVA NSGTEMLRFL DAIHDVARRS IPEDEMLWPL
110 120 130 140 150
SMPPQLPTKD EEIKIAKLDQ YDAVLYRRYL AKEYGKRKQM VSGIHFNFEY
160 170 180 190 200
DQALIQQLYD EQSEVTDCKQ FKTKVYMKVA RNFLRYRWLI TYLFGASPVS
210 220 230 240 250
EDRYFRVYDD QPQEPVRSIR NSTYGYRNHD NVKVSYASLE RYLEDIHRMV
260 270 280 290 300
ENGLLSEEKE FYAPVRLRGG KQMSDLPKTG IRYIELRNLD LNPFSRLGIV
310 320 330 340 350
EDTVDFLHYF MLYLLWTDEK EEADEWVKTG DIFNEQVALG HPHETIKLIA
360 370 380 390 400
EGDRIFSEMI DMLDALGIRK GKEVVGKYYQ QLRNPQDTVS GKMWTIIQEN
410 420 430 440 450
SNSELGNIFG NQYQSMAFER PYQLAGFREM ELSTQIFLFD AIQKGLEIEI
460 470 480 490 500
LDEQEQFLKL QHGEHIEYVK NANMTSKDNY VVPLIMENKT VTKKILSAAG
510 520 530 540 550
FHVPGGEEFS SFIEAQEAHL RYANKAFVVK PKSTNYGLGI TIFKEGASLE
560 570 580 590 600
DFTEALRIAF KEDTAVLIEE FLPGTEYRFF VLDNDVKAIM LRVPANVTGD
610 620 630 640 650
GKHTVEELVA AKNSDPLRGT NHRAPLELIQ LNDLEKLMLK EQGLTIYSVP
660 670 680 690 700
EKEQIVYLRE NSNVSTGGDS IDMTDVIDDS YKQIAIEAVA ALGAKICGID
710 720 730 740 750
LIIPDKDVKG TRDSLTYGII EANFNPAMHM HVYPYAGQGR RLTMDVLKLL

YPEVVQ
Length:756
Mass (Da):87,212
Last modified:June 1, 2003 - v1
Checksum:iD282F6561A9D07A4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016830 Genomic DNA. Translation: AAO82770.1.
RefSeqiNP_816700.1. NC_004668.1.

Genome annotation databases

EnsemblBacteriaiAAO82770; AAO82770; EF_3089.
GeneIDi1201934.
KEGGiefa:EF3089.
PATRICi21856337. VBIEntFae7065_2881.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016830 Genomic DNA. Translation: AAO82770.1 .
RefSeqi NP_816700.1. NC_004668.1.

3D structure databases

ProteinModelPortali Q82ZG8.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 226185.EF3089.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAO82770 ; AAO82770 ; EF_3089 .
GeneIDi 1201934.
KEGGi efa:EF3089.
PATRICi 21856337. VBIEntFae7065_2881.

Phylogenomic databases

eggNOGi COG1181.
KOi K01919.
OMAi RYLAKEY.
OrthoDBi EOG6BKJ7H.

Enzyme and pathway databases

UniPathwayi UPA00142 ; UER00209 .
UPA00142 ; UER00210 .
BioCyci EFAE226185:GHI1-3016-MONOMER.

Family and domain databases

Gene3Di 3.30.1490.20. 1 hit.
3.30.470.20. 3 hits.
HAMAPi MF_00782. Glut_biosynth.
InterProi IPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR007370. Glu_cys_ligase.
IPR006335. Glut_biosynth.
[Graphical view ]
Pfami PF04262. Glu_cys_ligase. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR01435. glu_cys_lig_rel. 1 hit.
PROSITEi PS50975. ATP_GRASP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700802 / V583.

Entry informationi

Entry nameiGSHAB_ENTFA
AccessioniPrimary (citable) accession number: Q82ZG8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 24, 2004
Last sequence update: June 1, 2003
Last modified: November 26, 2014
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3