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Q82ZG8

- GSHAB_ENTFA

UniProt

Q82ZG8 - GSHAB_ENTFA

Protein

Glutathione biosynthesis bifunctional protein GshAB

Gene

gshAB

Organism
Enterococcus faecalis (strain ATCC 700802 / V583)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 81 (01 Oct 2014)
      Sequence version 1 (01 Jun 2003)
      Previous versions | rss
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    Functioni

    Synthesizes glutathione from L-glutamate and L-cysteine via gamma-L-glutamyl-L-cysteine.UniRule annotation

    Catalytic activityi

    ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine.UniRule annotation
    ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione.UniRule annotation

    Cofactori

    Binds 2 magnesium or manganese ions per subunit.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi700 – 7001Magnesium or manganese 1UniRule annotation
    Metal bindingi721 – 7211Magnesium or manganese 1UniRule annotation
    Metal bindingi721 – 7211Magnesium or manganese 2UniRule annotation
    Metal bindingi723 – 7231Magnesium or manganese 2UniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi520 – 57859ATPUniRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. glutamate-cysteine ligase activity Source: UniProtKB-HAMAP
    3. glutathione synthase activity Source: UniProtKB-HAMAP
    4. magnesium ion binding Source: UniProtKB-HAMAP
    5. manganese ion binding Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Glutathione biosynthesis

    Keywords - Ligandi

    ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciEFAE226185:GHI1-3016-MONOMER.
    UniPathwayiUPA00142; UER00209.
    UPA00142; UER00210.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutathione biosynthesis bifunctional protein GshABUniRule annotation
    Alternative name(s):
    Gamma-GCS-GSUniRule annotation
    Short name:
    GCS-GSUniRule annotation
    Including the following 2 domains:
    Glutamate--cysteine ligaseUniRule annotation (EC:6.3.2.2UniRule annotation)
    Alternative name(s):
    Gamma-ECSUniRule annotation
    Short name:
    GCSUniRule annotation
    Gamma-glutamylcysteine synthetaseUniRule annotation
    Glutathione synthetaseUniRule annotation (EC:6.3.2.3UniRule annotation)
    Alternative name(s):
    GSH synthetaseUniRule annotation
    Short name:
    GSUniRule annotation
    Short name:
    GSH-SUniRule annotation
    Short name:
    GSHaseUniRule annotation
    Glutathione synthaseUniRule annotation
    Gene namesi
    Name:gshABUniRule annotation
    Synonyms:gshFUniRule annotation
    Ordered Locus Names:EF_3089
    OrganismiEnterococcus faecalis (strain ATCC 700802 / V583)
    Taxonomic identifieri226185 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesEnterococcaceaeEnterococcus
    ProteomesiUP000001415: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 756756Glutathione biosynthesis bifunctional protein GshABPRO_0000192551Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi226185.EF3089.

    Structurei

    3D structure databases

    ProteinModelPortaliQ82ZG8.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini493 – 751259ATP-graspUniRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 338338Glutamate--cysteine ligaseAdd
    BLAST

    Sequence similaritiesi

    In the N-terminal section; belongs to the glutamate--cysteine ligase type 1 family. Type 2 subfamily.UniRule annotation
    Contains 1 ATP-grasp domain.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1181.
    KOiK01919.
    OMAiRYLAKEY.
    OrthoDBiEOG6BKJ7H.

    Family and domain databases

    Gene3Di3.30.1490.20. 1 hit.
    3.30.470.20. 3 hits.
    HAMAPiMF_00782. Glut_biosynth.
    InterProiIPR011761. ATP-grasp.
    IPR013815. ATP_grasp_subdomain_1.
    IPR013816. ATP_grasp_subdomain_2.
    IPR007370. Glu_cys_ligase.
    IPR006335. Glut_biosynth.
    [Graphical view]
    PfamiPF04262. Glu_cys_ligase. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR01435. glu_cys_lig_rel. 1 hit.
    PROSITEiPS50975. ATP_GRASP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q82ZG8-1 [UniParc]FASTAAdd to Basket

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    MNYRELMQKK NVRPYVLMAR FGLEKENQRS TREGLLATTE HPTVFGNRSY    50
    HPYIQTDFSE TQLELITPVA NSGTEMLRFL DAIHDVARRS IPEDEMLWPL 100
    SMPPQLPTKD EEIKIAKLDQ YDAVLYRRYL AKEYGKRKQM VSGIHFNFEY 150
    DQALIQQLYD EQSEVTDCKQ FKTKVYMKVA RNFLRYRWLI TYLFGASPVS 200
    EDRYFRVYDD QPQEPVRSIR NSTYGYRNHD NVKVSYASLE RYLEDIHRMV 250
    ENGLLSEEKE FYAPVRLRGG KQMSDLPKTG IRYIELRNLD LNPFSRLGIV 300
    EDTVDFLHYF MLYLLWTDEK EEADEWVKTG DIFNEQVALG HPHETIKLIA 350
    EGDRIFSEMI DMLDALGIRK GKEVVGKYYQ QLRNPQDTVS GKMWTIIQEN 400
    SNSELGNIFG NQYQSMAFER PYQLAGFREM ELSTQIFLFD AIQKGLEIEI 450
    LDEQEQFLKL QHGEHIEYVK NANMTSKDNY VVPLIMENKT VTKKILSAAG 500
    FHVPGGEEFS SFIEAQEAHL RYANKAFVVK PKSTNYGLGI TIFKEGASLE 550
    DFTEALRIAF KEDTAVLIEE FLPGTEYRFF VLDNDVKAIM LRVPANVTGD 600
    GKHTVEELVA AKNSDPLRGT NHRAPLELIQ LNDLEKLMLK EQGLTIYSVP 650
    EKEQIVYLRE NSNVSTGGDS IDMTDVIDDS YKQIAIEAVA ALGAKICGID 700
    LIIPDKDVKG TRDSLTYGII EANFNPAMHM HVYPYAGQGR RLTMDVLKLL 750
    YPEVVQ 756
    Length:756
    Mass (Da):87,212
    Last modified:June 1, 2003 - v1
    Checksum:iD282F6561A9D07A4
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE016830 Genomic DNA. Translation: AAO82770.1.
    RefSeqiNP_816700.1. NC_004668.1.

    Genome annotation databases

    EnsemblBacteriaiAAO82770; AAO82770; EF_3089.
    GeneIDi1201934.
    KEGGiefa:EF3089.
    PATRICi21856337. VBIEntFae7065_2881.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE016830 Genomic DNA. Translation: AAO82770.1 .
    RefSeqi NP_816700.1. NC_004668.1.

    3D structure databases

    ProteinModelPortali Q82ZG8.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 226185.EF3089.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAO82770 ; AAO82770 ; EF_3089 .
    GeneIDi 1201934.
    KEGGi efa:EF3089.
    PATRICi 21856337. VBIEntFae7065_2881.

    Phylogenomic databases

    eggNOGi COG1181.
    KOi K01919.
    OMAi RYLAKEY.
    OrthoDBi EOG6BKJ7H.

    Enzyme and pathway databases

    UniPathwayi UPA00142 ; UER00209 .
    UPA00142 ; UER00210 .
    BioCyci EFAE226185:GHI1-3016-MONOMER.

    Family and domain databases

    Gene3Di 3.30.1490.20. 1 hit.
    3.30.470.20. 3 hits.
    HAMAPi MF_00782. Glut_biosynth.
    InterProi IPR011761. ATP-grasp.
    IPR013815. ATP_grasp_subdomain_1.
    IPR013816. ATP_grasp_subdomain_2.
    IPR007370. Glu_cys_ligase.
    IPR006335. Glut_biosynth.
    [Graphical view ]
    Pfami PF04262. Glu_cys_ligase. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR01435. glu_cys_lig_rel. 1 hit.
    PROSITEi PS50975. ATP_GRASP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 700802 / V583.

    Entry informationi

    Entry nameiGSHAB_ENTFA
    AccessioniPrimary (citable) accession number: Q82ZG8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 24, 2004
    Last sequence update: June 1, 2003
    Last modified: October 1, 2014
    This is version 81 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3