SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q82ZG8

- GSHAB_ENTFA

UniProt

Q82ZG8 - GSHAB_ENTFA

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Glutathione biosynthesis bifunctional protein GshAB
Gene
gshAB, gshF, EF_3089
Organism
Enterococcus faecalis (strain ATCC 700802 / V583)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Synthesizes glutathione from L-glutamate and L-cysteine via gamma-L-glutamyl-L-cysteine By similarity.UniRule annotation

Catalytic activityi

ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine.UniRule annotation
ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione.UniRule annotation

Cofactori

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi700 – 7001Magnesium or manganese 1 By similarity
Metal bindingi721 – 7211Magnesium or manganese 1 By similarity
Metal bindingi721 – 7211Magnesium or manganese 2 By similarity
Metal bindingi723 – 7231Magnesium or manganese 2 By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi520 – 57859ATP By similarity
Add
BLAST

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. glutamate-cysteine ligase activity Source: UniProtKB-HAMAP
  3. glutathione synthase activity Source: UniProtKB-HAMAP
  4. magnesium ion binding Source: UniProtKB-HAMAP
  5. manganese ion binding Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Glutathione biosynthesis

    Keywords - Ligandi

    ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciEFAE226185:GHI1-3016-MONOMER.
    UniPathwayiUPA00142; UER00209.
    UPA00142; UER00210.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutathione biosynthesis bifunctional protein GshAB
    Alternative name(s):
    Gamma-GCS-GS
    Short name:
    GCS-GS
    Including the following 2 domains:
    Glutamate--cysteine ligase (EC:6.3.2.2)
    Alternative name(s):
    Gamma-ECS
    Short name:
    GCS
    Gamma-glutamylcysteine synthetase
    Glutathione synthetase (EC:6.3.2.3)
    Alternative name(s):
    GSH synthetase
    Short name:
    GS
    Short name:
    GSH-S
    Short name:
    GSHase
    Glutathione synthase
    Gene namesi
    Name:gshAB
    Synonyms:gshF
    Ordered Locus Names:EF_3089
    OrganismiEnterococcus faecalis (strain ATCC 700802 / V583)
    Taxonomic identifieri226185 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesEnterococcaceaeEnterococcus
    ProteomesiUP000001415: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 756756Glutathione biosynthesis bifunctional protein GshABUniRule annotation
    PRO_0000192551Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer By similarity.UniRule annotation

    Protein-protein interaction databases

    STRINGi226185.EF3089.

    Structurei

    3D structure databases

    ProteinModelPortaliQ82ZG8.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini493 – 751259ATP-grasp
    Add
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 338338Glutamate--cysteine ligaseUniRule annotation
    Add
    BLAST

    Sequence similaritiesi

    In the N-terminal section; belongs to the glutamate--cysteine ligase type 1 family. Type 2 subfamily.
    Contains 1 ATP-grasp domain.

    Phylogenomic databases

    eggNOGiCOG1181.
    KOiK01919.
    OMAiRYLAKEY.
    OrthoDBiEOG6BKJ7H.

    Family and domain databases

    Gene3Di3.30.1490.20. 1 hit.
    3.30.470.20. 3 hits.
    HAMAPiMF_00782. Glut_biosynth.
    InterProiIPR011761. ATP-grasp.
    IPR013815. ATP_grasp_subdomain_1.
    IPR013816. ATP_grasp_subdomain_2.
    IPR007370. Glu_cys_ligase.
    IPR006335. Glut_biosynth.
    [Graphical view]
    PfamiPF04262. Glu_cys_ligase. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR01435. glu_cys_lig_rel. 1 hit.
    PROSITEiPS50975. ATP_GRASP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q82ZG8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNYRELMQKK NVRPYVLMAR FGLEKENQRS TREGLLATTE HPTVFGNRSY    50
    HPYIQTDFSE TQLELITPVA NSGTEMLRFL DAIHDVARRS IPEDEMLWPL 100
    SMPPQLPTKD EEIKIAKLDQ YDAVLYRRYL AKEYGKRKQM VSGIHFNFEY 150
    DQALIQQLYD EQSEVTDCKQ FKTKVYMKVA RNFLRYRWLI TYLFGASPVS 200
    EDRYFRVYDD QPQEPVRSIR NSTYGYRNHD NVKVSYASLE RYLEDIHRMV 250
    ENGLLSEEKE FYAPVRLRGG KQMSDLPKTG IRYIELRNLD LNPFSRLGIV 300
    EDTVDFLHYF MLYLLWTDEK EEADEWVKTG DIFNEQVALG HPHETIKLIA 350
    EGDRIFSEMI DMLDALGIRK GKEVVGKYYQ QLRNPQDTVS GKMWTIIQEN 400
    SNSELGNIFG NQYQSMAFER PYQLAGFREM ELSTQIFLFD AIQKGLEIEI 450
    LDEQEQFLKL QHGEHIEYVK NANMTSKDNY VVPLIMENKT VTKKILSAAG 500
    FHVPGGEEFS SFIEAQEAHL RYANKAFVVK PKSTNYGLGI TIFKEGASLE 550
    DFTEALRIAF KEDTAVLIEE FLPGTEYRFF VLDNDVKAIM LRVPANVTGD 600
    GKHTVEELVA AKNSDPLRGT NHRAPLELIQ LNDLEKLMLK EQGLTIYSVP 650
    EKEQIVYLRE NSNVSTGGDS IDMTDVIDDS YKQIAIEAVA ALGAKICGID 700
    LIIPDKDVKG TRDSLTYGII EANFNPAMHM HVYPYAGQGR RLTMDVLKLL 750
    YPEVVQ 756
    Length:756
    Mass (Da):87,212
    Last modified:June 1, 2003 - v1
    Checksum:iD282F6561A9D07A4
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE016830 Genomic DNA. Translation: AAO82770.1.
    RefSeqiNP_816700.1. NC_004668.1.

    Genome annotation databases

    EnsemblBacteriaiAAO82770; AAO82770; EF_3089.
    GeneIDi1201934.
    KEGGiefa:EF3089.
    PATRICi21856337. VBIEntFae7065_2881.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE016830 Genomic DNA. Translation: AAO82770.1 .
    RefSeqi NP_816700.1. NC_004668.1.

    3D structure databases

    ProteinModelPortali Q82ZG8.
    ModBasei Search...

    Protein-protein interaction databases

    STRINGi 226185.EF3089.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAO82770 ; AAO82770 ; EF_3089 .
    GeneIDi 1201934.
    KEGGi efa:EF3089.
    PATRICi 21856337. VBIEntFae7065_2881.

    Phylogenomic databases

    eggNOGi COG1181.
    KOi K01919.
    OMAi RYLAKEY.
    OrthoDBi EOG6BKJ7H.

    Enzyme and pathway databases

    UniPathwayi UPA00142 ; UER00209 .
    UPA00142 ; UER00210 .
    BioCyci EFAE226185:GHI1-3016-MONOMER.

    Family and domain databases

    Gene3Di 3.30.1490.20. 1 hit.
    3.30.470.20. 3 hits.
    HAMAPi MF_00782. Glut_biosynth.
    InterProi IPR011761. ATP-grasp.
    IPR013815. ATP_grasp_subdomain_1.
    IPR013816. ATP_grasp_subdomain_2.
    IPR007370. Glu_cys_ligase.
    IPR006335. Glut_biosynth.
    [Graphical view ]
    Pfami PF04262. Glu_cys_ligase. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR01435. glu_cys_lig_rel. 1 hit.
    PROSITEi PS50975. ATP_GRASP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 700802 / V583.

    Entry informationi

    Entry nameiGSHAB_ENTFA
    AccessioniPrimary (citable) accession number: Q82ZG8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 24, 2004
    Last sequence update: June 1, 2003
    Last modified: May 14, 2014
    This is version 80 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

    Similar proteinsi