Reviewed, UniProtKB/Swiss-Prot Q82ZC9 (UXUA_ENTFA)

Names and origin

Protein names

Recommended name:
    Mannonate dehydratase
    EC=4.2.1.8
Alternative name(s):
    D-mannonate hydrolase

Gene names

Name:	uxuA
Ordered Locus Names:	EF_3135

Organism

Enterococcus faecalis (Streptococcus faecalis) [Complete proteome] [HAMAP]

Taxonomic identifier

1351 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Enterococcaceae › Enterococcus

Protein attributes

Sequence length	357 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	D-mannonate = 2-dehydro-3-deoxy-D-gluconate + H2O. HAMAP MF_00106
Pathway	Carbohydrate metabolism; pentose and glucuronate interconversion. HAMAP MF_00106
Sequence similarities	Belongs to the mannonate dehydratase family.

Ontologies

Keywords
Molecular function	Lyase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	glucuronate catabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	mannonate dehydratase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 357

357

Mannonate dehydratase HAMAP MF_00106

PRO_0000170673

Secondary structure

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357

Helix Strand Turn

Details...

Turn

10 – 12

3

Helix

17 – 20

4

Beta strand

28 – 31

4

Beta strand

34 – 36

3

Helix

44 – 56

13

Beta strand

60 – 64

5

Helix

71 – 75

5

Helix

80 – 96

17

Beta strand

101 – 104

4

Beta strand

115 – 120

6

Beta strand

126 – 131

6

Helix

132 – 136

5

Helix

140 – 146

7

Helix

161 – 173

13

Helix

179 – 200

22

Beta strand

203 – 206

4

Beta strand

210 – 213

4

Helix

225 – 234

10

Helix

248 – 251

4

Helix

257 – 264

8

Helix

265 – 267

3

Beta strand

271 – 273

3

Beta strand

276 – 278

3

Beta strand

280 – 286

7

Helix

291 – 293

3

Beta strand

294 – 296

3

Helix

298 – 308

11

Beta strand

312 – 316

5

Beta strand

331 – 333

3

Helix

334 – 340

7

Helix

342 – 351

10

Sequences

	Sequence		Length	Mass (Da)
	Q82ZC9-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 4CA7E9E55D33A061	FASTA	357	40,282
	10 20 30 40 50 60 MKWGFRWYGA AGDAIPLKHI RQIPGITGVV GTLLNKLPGD VWTVAEIQAL KQSVEQEGLA 70 80 90 100 110 120 LLGIESVAIH DAIKAGTDQR DHYIDNYRQT LRNLGKCGIS LVCYSFKPIF GWAKTDLAYE 130 140 150 160 170 180 NEDGSLSLLF DQAVVENMQP EDMYQLIHSQ SKGFRLPGWE EERLQQFQEL KAMYAGVTEE 190 200 210 220 230 240 DLVENLRYFL ERVIPVCEEE NIKMGIHPDD PPWEIFGLPR ITKNLADLKR ILSLVDSPAN 250 260 270 280 290 300 GITFCTGSLG ADPTNDLPTM IREIGHRINF VHFRNVKYLG EHRFEETAHP SVAGSLDMAE 310 320 330 340 350 LMQALVDVGY EGVIRPDHGR AIWDEKAMPG YGLYDRAMGL TYIQGLYEAT KAKQNRK

References

[1]

"Role of mobile DNA in the evolution of vancomycin-resistant Enterococcus faecalis." Paulsen I.T., Banerjei L., Myers G.S.A., Nelson K.E., Seshadri R., Read T.D., Fouts D.E., Eisen J.A., Gill S.R., Heidelberg J.F., Tettelin H., Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J., Daugherty S.C., DeBoy R.T., Durkin S.A. , Kolonay J.F., Madupu R., Nelson W.C., Vamathevan J.J., Tran B., Upton J., Hansen T., Shetty J., Khouri H.M., Utterback T.R., Radune D., Ketchum K.A., Dougherty B.A., Fraser C.M. Science 299:2071-2074(2003) [PubMed: 12663927] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: V583 / ATCC 700802.

Cross-references

Sequence databases

EMBLGenBankDDBJ

AE016830 Genomic DNA. Translation: AAO82813.1.

RefSeq

NP_816743.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1TZ9	X-ray	2.90	A/B	1-357	[»]

ModBase

Search...

Genome annotation databases

GeneID

1201978.

GenomeReviews

Gene locus EF_3135 in contig AE016830_GR.

KEGG

efa:EF3135.

NMPDR

fig|226185.1.peg.2928.

TIGR

EF_3135.

Phylogenomic databases

HOGENOM

Q82ZC9.

OMA

GWEEERL.

Enzyme and pathway databases

BioCyc

EFAE226185:EF_3135-MON.

BRENDA

4.2.1.8. 704.

Family and domain databases

HAMAP

MF_00106.
[Tree]

InterPro

IPR004628. Man_deHydtase.
IPR013022. Xyl_isomerase-like_TIM-brl.
[Graphical view]

Gene3D

G3DSA:3.20.20.150. Xyl_isomerase-like_TIM-brl. 1 hit.

Pfam

PF03786. UxuA. 1 hit.
[Graphical view]

PIRSF

PIRSF016049. Man_dehyd. 1 hit.

TIGRFAMs

TIGR00695. uxuA. 1 hit.

ProtoNet

Search...

Entry information

Entry name

UXUA_ENTFA

Accession

Primary (citable) accession number: Q82ZC9

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 11, 2003
Last sequence update:	June 1, 2003
Last modified:	November 3, 2009
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)