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Protein

Polyribonucleotide nucleotidyltransferase

Gene

pnp

Organism
Nitrosomonas europaea (strain ATCC 19718 / CIP 103999 / KCTC 2705 / NBRC 14298)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction.UniRule annotation

Catalytic activityi

RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi486MagnesiumUniRule annotation1
Metal bindingi492MagnesiumUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Polyribonucleotide nucleotidyltransferaseUniRule annotation (EC:2.7.7.8UniRule annotation)
Alternative name(s):
Polynucleotide phosphorylaseUniRule annotation
Short name:
PNPaseUniRule annotation
Gene namesi
Name:pnpUniRule annotation
Ordered Locus Names:NE0172
OrganismiNitrosomonas europaea (strain ATCC 19718 / CIP 103999 / KCTC 2705 / NBRC 14298)
Taxonomic identifieri228410 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaNitrosomonadalesNitrosomonadaceaeNitrosomonas
Proteomesi
  • UP000001416 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003297371 – 708Polyribonucleotide nucleotidyltransferaseAdd BLAST708

Interactioni

Protein-protein interaction databases

STRINGi228410.NE0172.

Structurei

3D structure databases

ProteinModelPortaliQ82XT0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini553 – 612KHUniRule annotationAdd BLAST60
Domaini622 – 690S1 motifUniRule annotationAdd BLAST69

Sequence similaritiesi

Belongs to the polyribonucleotide nucleotidyltransferase family.UniRule annotation
Contains 1 KH domain.UniRule annotation
Contains 1 S1 motif domain.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C62. Bacteria.
COG1185. LUCA.
HOGENOMiHOG000218327.
KOiK00962.
OMAiRFMFHYN.
OrthoDBiPOG091H00M0.
PhylomeDBiQ82XT0.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase. 1 hit.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
IPR003029. S1_domain.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 1 hit.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q82XT0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKPIKKSITY GRHTLTIETG EIAKQAHGAV IVSMDDTVVL VTAVGDKKTK
60 70 80 90 100
PGQDFFPLTV DYQEKFYSAG RIPGSFFKRE GRPSEKETLT SRLIDRPIRP
110 120 130 140 150
LFPDGFYNEV QVVAMVLSSD TEIDADIPAM IGTSAALILS GIPFDGPVGA
160 170 180 190 200
ARVGYINNEY ILNPTTTQLK ESQLNLVVAG TQKAVLMVES EADELSEDVM
210 220 230 240 250
LGAVTYGHDQ MQQVIDMINE LADEAGVTAW DWQPAEQDLS LVEKVTQLAE
260 270 280 290 300
ADLRNAFRLK QKSARVEAIN EIWQRVFTEL KVGTEEGPSE QAVREIGFAL
310 320 330 340 350
EARIVRNSIL DGESRIDGRG TRTVRPITIR HGVLPRTHGS ALFTRGETQA
360 370 380 390 400
LAITTLGTAR DEQKIDALQG DYSERFMLHY NMPPFATGET GRVGTPKRRE
410 420 430 440 450
IGHGRLAKRA LLAVIPPVEE FGYSMRVVSE VTESNGSSSM ASVCGGCLSL
460 470 480 490 500
MDAGVPLKAH VAGIAMGLIK EGNRFAVLTD ILGDEDHLGD MDFKVAGTEH
510 520 530 540 550
GITALQMDIK IQGITKEIMQ VALLQAKEGR LHILEIMKQS LPIARESISV
560 570 580 590 600
HAPRIIKFKI NPEKIRDVIG KGGAVIRALT EETGTTIDIS DDGSVTIACV
610 620 630 640 650
SSEGGEQARK RIEDITADVE VGRIYEGTVL KLLDFGAIVS VLPGKDGLLH
660 670 680 690 700
ISQIANERVE NVADHLKEGQ TVRVKVLEAD EKGRLRLSMK AASISTEITA

DNPDNTEK
Length:708
Mass (Da):77,152
Last modified:June 1, 2003 - v1
Checksum:iE2A613FE45178CA0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL954747 Genomic DNA. Translation: CAD84083.1.
RefSeqiWP_011110817.1. NC_004757.1.

Genome annotation databases

EnsemblBacteriaiCAD84083; CAD84083; NE0172.
KEGGineu:NE0172.
PATRICi22711155. VBINitEur56163_0194.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL954747 Genomic DNA. Translation: CAD84083.1.
RefSeqiWP_011110817.1. NC_004757.1.

3D structure databases

ProteinModelPortaliQ82XT0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi228410.NE0172.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAD84083; CAD84083; NE0172.
KEGGineu:NE0172.
PATRICi22711155. VBINitEur56163_0194.

Phylogenomic databases

eggNOGiENOG4105C62. Bacteria.
COG1185. LUCA.
HOGENOMiHOG000218327.
KOiK00962.
OMAiRFMFHYN.
OrthoDBiPOG091H00M0.
PhylomeDBiQ82XT0.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase. 1 hit.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
IPR003029. S1_domain.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 1 hit.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPNP_NITEU
AccessioniPrimary (citable) accession number: Q82XT0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: June 1, 2003
Last modified: November 2, 2016
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.