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Q82XE8 (PGK_NITEU) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 60. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phosphoglycerate kinase

EC=2.7.2.3
Gene names
Name:pgk
Ordered Locus Names:NE0326
OrganismNitrosomonas europaea (strain ATCC 19718 / NBRC 14298) [Complete proteome] [HAMAP]
Taxonomic identifier228410 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaNitrosomonadalesNitrosomonadaceaeNitrosomonas

Protein attributes

Sequence length392 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate. HAMAP-Rule MF_00145

Pathway

Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 2/5. HAMAP-Rule MF_00145

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00145

Subcellular location

Cytoplasm Potential HAMAP-Rule MF_00145.

Sequence similarities

Belongs to the phosphoglycerate kinase family.

Ontologies

Keywords
   Biological processGlycolysis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionKinase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processglycolysis

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

phosphoglycerate kinase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 392392Phosphoglycerate kinase HAMAP-Rule MF_00145
PRO_0000145977

Regions

Nucleotide binding346 – 3494ATP By similarity
Region21 – 233Substrate binding By similarity
Region59 – 624Substrate binding By similarity

Sites

Binding site361Substrate By similarity
Binding site1141Substrate By similarity
Binding site1471Substrate By similarity
Binding site1981ATP By similarity
Binding site3201ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q82XE8 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: 77174FD554E0F837

FASTA39241,902
        10         20         30         40         50         60 
MSVIKLVDLD LKNKRVFIRA DLNVPVKDGK VTSDARITAS MATINHCLKQ GAKVMVTSHL 

        70         80         90        100        110        120 
GRPEEGVWTE ENSLQPVADD IARRLGKPVR LIKDWVEGGF EVASGELVVL ENCRINKGEK 

       130        140        150        160        170        180 
KNLEETAKKY ASLCDVFVMD AFGTAHRAEA STHGIAKYAP IACAGILLTE ELDALTKALH 

       190        200        210        220        230        240 
QPAHPLVAIV GGSKVSTKLT VLESLAEKVD QLVVGGGIAN TFLKAAGNNI GKSLCEDELV 

       250        260        270        280        290        300 
PVAKSLMDKM NKRNATIPIA VDVVVGKKFA EDEAAVLKAA NAVSDDDMIF DIGPESAQEL 

       310        320        330        340        350        360 
VDIIMKAGTV VWNGPVGVFE FDQFGEGTRA IAKAIAETDA FTLAGGGDTI AAIQKYDIYD 

       370        380        390 
KVSYISTAGG AFLEFLEGKK LPAVEILELR AK 

« Hide

References

[1]"Complete genome sequence of the ammonia-oxidizing bacterium and obligate chemolithoautotroph Nitrosomonas europaea."
Chain P., Lamerdin J.E., Larimer F.W., Regala W., Lao V., Land M.L., Hauser L., Hooper A.B., Klotz M.G., Norton J., Sayavedra-Soto L.A., Arciero D.M., Hommes N.G., Whittaker M.M., Arp D.J.
J. Bacteriol. 185:2759-2773(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 19718 / NBRC 14298.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AL954747 Genomic DNA. Translation: CAD84237.1.
RefSeqNP_840413.1. NC_004757.1.

3D structure databases

ProteinModelPortalQ82XE8.
SMRQ82XE8. Positions 2-392.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING228410.NE0326.

Proteomic databases

PRIDEQ82XE8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAD84237; CAD84237; NE0326.
GeneID1081255.
KEGGneu:NE0326.
PATRIC22711499. VBINitEur56163_0364.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0126.
HOGENOMHOG000227107.
KOK00927.
OMADMIFDIG.
OrthoDBEOG64N9Z0.
ProtClustDBPRK00073.

Enzyme and pathway databases

BioCycNEUR228410:GJNO-336-MONOMER.
UniPathwayUPA00109; UER00185.

Family and domain databases

Gene3D3.40.50.1260. 1 hit.
3.40.50.1270. 1 hit.
HAMAPMF_00145. Phosphoglyc_kinase.
InterProIPR001576. Phosphoglycerate_kinase.
IPR015901. Phosphoglycerate_kinase_C.
IPR015911. Phosphoglycerate_kinase_CS.
IPR015824. Phosphoglycerate_kinase_N.
[Graphical view]
PANTHERPTHR11406. PTHR11406. 1 hit.
PfamPF00162. PGK. 1 hit.
[Graphical view]
PIRSFPIRSF000724. Pgk. 1 hit.
PRINTSPR00477. PHGLYCKINASE.
SUPFAMSSF53748. SSF53748. 1 hit.
PROSITEPS00111. PGLYCERATE_KINASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePGK_NITEU
AccessionPrimary (citable) accession number: Q82XE8
Entry history
Integrated into UniProtKB/Swiss-Prot: April 26, 2004
Last sequence update: June 1, 2003
Last modified: February 19, 2014
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways