Q82X99 (RNPA_NITEU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribonuclease P protein component
Short name=RNase P protein
Short name=RNaseP protein
EC=3.1.26.5

Alternative name(s):
Protein C5

Gene names

Name:	rnpA
Ordered Locus Names:	NE0389

Organism

Nitrosomonas europaea (strain ATCC 19718 / NBRC 14298) [Complete proteome] [HAMAP]

Taxonomic identifier

228410 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Betaproteobacteria › Nitrosomonadales › Nitrosomonadaceae › Nitrosomonas › Nitrosomonas europaea

Protein attributes

Sequence length	119 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	RNaseP catalyzes the removal of the 5'-leader sequence from pre-tRNA to produce the mature 5'-terminus. It can also cleave other RNA substrates such as 4.5S RNA. The protein component plays an auxiliary but essential role in vivo by binding to the 5'-leader sequence and broadening the substrate specificity of the ribozyme By similarity. HAMAP-Rule MF_00227
Catalytic activity	Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor. HAMAP-Rule MF_00227
Subunit structure	Consists of a catalytic RNA component (M1 or RnpB) and a protein subunit By similarity.
Sequence similarities	Belongs to the RnpA family.

Ontologies

Keywords
Biological process	tRNA processing
Ligand	RNA-binding
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC tRNA 5'-leader removal Inferred from electronic annotation. Source: HAMAP
Molecular_function	ribonuclease P activity Inferred from electronic annotation. Source: HAMAP tRNA binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 119	119	Ribonuclease P protein component HAMAP-Rule MF_00227		PRO_0000198499

Sequences

	Sequence		Length	Mass (Da)
	Q82X99 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: CBB97FECC340737D	FASTA	119	14,279
	10 20 30 40 50 60 MTTRQICTLP RQCKLRKADE FRAVLRNRIV FESLSLRLYV KPIDVDYARI GLIVAKRVER 70 80 90 100 110 KAVRRNRIKR LIREAFRRHR QMLMGLDCVM QLRHPVELLD STRIYQEAVM LFNKAARQL

References

[1]

"Complete genome sequence of the ammonia-oxidizing bacterium and obligate chemolithoautotroph Nitrosomonas europaea." Chain P., Lamerdin J.E., Larimer F.W., Regala W., Lao V., Land M.L., Hauser L., Hooper A.B., Klotz M.G., Norton J., Sayavedra-Soto L.A., Arciero D.M., Hommes N.G., Whittaker M.M., Arp D.J. J. Bacteriol. 185:2759-2773(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 19718 / NBRC 14298.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL954747 Genomic DNA. Translation: CAD84300.1.
RefSeq	NP_840476.1. NC_004757.1.
3D structure databases
ProteinModelPortal	Q82X99.
ModBase	Search...
Protein-protein interaction databases
STRING	228410.NE0389.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAD84300; CAD84300; NE0389.
GeneID	1081322.
KEGG	neu:NE0389.
PATRIC	22711643. VBINitEur56163_0432.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	NOG127496.
HOGENOM	HOG000266301.
KO	K03536.
OMA	DCVMQLR.
ProtClustDB	CLSK585298.
Enzyme and pathway databases
BioCyc	NEUR228410:GJNO-403-MONOMER.
Family and domain databases
Gene3D	3.30.230.10. 1 hit.
HAMAP	MF_00227. RNase_P.
InterPro	IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. IPR000100. RNase_P. [Graphical view]
Pfam	PF00825. Ribonuclease_P. 1 hit. [Graphical view]
ProDom	PD003629. Ribonuclease_P. 1 hit. [Graphical view] [Entries sharing at least one domain]
SUPFAM	SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR00188. rnpA. 1 hit.
PROSITE	PS00648. RIBONUCLEASE_P. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RNPA_NITEU

Accession

Primary (citable) accession number: Q82X99

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 29, 2004
Last sequence update:	June 1, 2003
Last modified:	May 1, 2013
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program