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Q82WB9

- MDH_NITEU

UniProt

Q82WB9 - MDH_NITEU

Protein

Malate dehydrogenase

Gene

mdh

Organism
Nitrosomonas europaea (strain ATCC 19718 / NBRC 14298)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 81 (01 Oct 2014)
      Sequence version 1 (01 Jun 2003)
      Previous versions | rss
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    Functioni

    Catalyzes the reversible oxidation of malate to oxaloacetate.UniRule annotation

    Catalytic activityi

    (S)-malate + NAD+ = oxaloacetate + NADH.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei92 – 921SubstrateUniRule annotation
    Binding sitei98 – 981SubstrateUniRule annotation
    Binding sitei105 – 1051NADUniRule annotation
    Binding sitei112 – 1121NADUniRule annotation
    Binding sitei131 – 1311SubstrateUniRule annotation
    Binding sitei162 – 1621SubstrateUniRule annotation
    Active sitei187 – 1871Proton acceptorUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi11 – 177NADUniRule annotation
    Nucleotide bindingi129 – 1313NADUniRule annotation

    GO - Molecular functioni

    1. L-malate dehydrogenase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. cellular carbohydrate metabolic process Source: InterPro
    2. malate metabolic process Source: InterPro
    3. tricarboxylic acid cycle Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Tricarboxylic acid cycle

    Keywords - Ligandi

    NAD

    Enzyme and pathway databases

    BioCyciNEUR228410:GJNO-793-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Malate dehydrogenaseUniRule annotation (EC:1.1.1.37UniRule annotation)
    Gene namesi
    Name:mdhUniRule annotation
    Ordered Locus Names:NE0773
    OrganismiNitrosomonas europaea (strain ATCC 19718 / NBRC 14298)
    Taxonomic identifieri228410 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaNitrosomonadalesNitrosomonadaceaeNitrosomonas
    ProteomesiUP000001416: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 327327Malate dehydrogenasePRO_0000113382Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi228410.NE0773.

    Structurei

    3D structure databases

    ProteinModelPortaliQ82WB9.
    SMRiQ82WB9. Positions 1-327.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the LDH/MDH superfamily. MDH type 2 family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0039.
    HOGENOMiHOG000220953.
    KOiK00024.
    OMAiIAPMIAR.
    OrthoDBiEOG6PP9Q2.
    PhylomeDBiQ82WB9.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    3.90.110.10. 1 hit.
    HAMAPiMF_01517. Malate_dehydrog_2.
    InterProiIPR001557. L-lactate/malate_DH.
    IPR022383. Lactate/malate_DH_C.
    IPR001236. Lactate/malate_DH_N.
    IPR015955. Lactate_DH/Glyco_Ohase_4_C.
    IPR010945. Malate_DH_type2.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PANTHERiPTHR23382. PTHR23382. 1 hit.
    PfamiPF02866. Ldh_1_C. 1 hit.
    PF00056. Ldh_1_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
    SUPFAMiSSF56327. SSF56327. 1 hit.
    TIGRFAMsiTIGR01759. MalateDH-SF1. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q82WB9-1 [UniParc]FASTAAdd to Basket

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    MSSPIRIAVT GAAGQISYSL LFRIAAGDML GSSQPVILQL LDIPESGKVL    50
    DGVLMELQDC AFPLLTDIIV THDPMIAFDQ ADIAILVGAR PRGKGMERKD 100
    LLQTNGEIFR EQGRALNQVV KRDAKILVVG NPANTNTLIT MKNAPDLSPE 150
    NFSGMLRLDH NRALSQVAMK LNQPVSHIRK MIVWGNHSST QFPDLSHAEI 200
    DHQKVIDLIK DQTWVENSFI PTVQNRGAVV IEARGLSSAA SAANAIIDHM 250
    RDWIFGTRDD DWITMGILSD GSYKIPKGVI YGFPVVCKNG GRKIVQGLEI 300
    SPFSRTRLDI AYDELTQELD SIKHLLL 327
    Length:327
    Mass (Da):36,000
    Last modified:June 1, 2003 - v1
    Checksum:i3C8D52C94C8F740F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL954747 Genomic DNA. Translation: CAD84684.1.
    RefSeqiNP_840847.1. NC_004757.1.
    WP_011111385.1. NC_004757.1.

    Genome annotation databases

    EnsemblBacteriaiCAD84684; CAD84684; NE0773.
    GeneIDi1081711.
    KEGGineu:NE0773.
    PATRICi22712522. VBINitEur56163_0865.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL954747 Genomic DNA. Translation: CAD84684.1 .
    RefSeqi NP_840847.1. NC_004757.1.
    WP_011111385.1. NC_004757.1.

    3D structure databases

    ProteinModelPortali Q82WB9.
    SMRi Q82WB9. Positions 1-327.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 228410.NE0773.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAD84684 ; CAD84684 ; NE0773 .
    GeneIDi 1081711.
    KEGGi neu:NE0773.
    PATRICi 22712522. VBINitEur56163_0865.

    Phylogenomic databases

    eggNOGi COG0039.
    HOGENOMi HOG000220953.
    KOi K00024.
    OMAi IAPMIAR.
    OrthoDBi EOG6PP9Q2.
    PhylomeDBi Q82WB9.

    Enzyme and pathway databases

    BioCyci NEUR228410:GJNO-793-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    3.90.110.10. 1 hit.
    HAMAPi MF_01517. Malate_dehydrog_2.
    InterProi IPR001557. L-lactate/malate_DH.
    IPR022383. Lactate/malate_DH_C.
    IPR001236. Lactate/malate_DH_N.
    IPR015955. Lactate_DH/Glyco_Ohase_4_C.
    IPR010945. Malate_DH_type2.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    PANTHERi PTHR23382. PTHR23382. 1 hit.
    Pfami PF02866. Ldh_1_C. 1 hit.
    PF00056. Ldh_1_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000102. Lac_mal_DH. 1 hit.
    SUPFAMi SSF56327. SSF56327. 1 hit.
    TIGRFAMsi TIGR01759. MalateDH-SF1. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Complete genome sequence of the ammonia-oxidizing bacterium and obligate chemolithoautotroph Nitrosomonas europaea."
      Chain P., Lamerdin J.E., Larimer F.W., Regala W., Lao V., Land M.L., Hauser L., Hooper A.B., Klotz M.G., Norton J., Sayavedra-Soto L.A., Arciero D.M., Hommes N.G., Whittaker M.M., Arp D.J.
      J. Bacteriol. 185:2759-2773(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 19718 / NBRC 14298.

    Entry informationi

    Entry nameiMDH_NITEU
    AccessioniPrimary (citable) accession number: Q82WB9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 7, 2004
    Last sequence update: June 1, 2003
    Last modified: October 1, 2014
    This is version 81 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3