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Protein

UvrABC system protein B

Gene

uvrB

Organism
Nitrosomonas europaea (strain ATCC 19718 / CIP 103999 / KCTC 2705 / NBRC 14298)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

The UvrABC repair system catalyzes the recognition and processing of DNA lesions. A damage recognition complex composed of 2 UvrA and 2 UvrB subunits scans DNA for abnormalities. Upon binding of the UvrA2B2 complex to a putative damaged site, the DNA wraps around one UvrB monomer. DNA wrap is dependent on ATP binding by UvrB and probably causes local melting of the DNA helix, facilitating insertion of UvrB beta-hairpin between the DNA strands. Then UvrB probes one DNA strand for the presence of a lesion. If a lesion is found the UvrA subunits dissociate and the UvrB-DNA preincision complex is formed. This complex is subsequently bound by UvrC and the second UvrB is released. If no lesion is found, the DNA wraps around the other UvrB subunit that will check the other stand for damage.UniRule annotation

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi44 – 51ATPUniRule annotation8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionExcision nuclease
Biological processDNA damage, DNA excision, DNA repair, SOS response
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
UvrABC system protein BUniRule annotation
Short name:
Protein UvrBUniRule annotation
Alternative name(s):
Excinuclease ABC subunit BUniRule annotation
Gene namesi
Name:uvrBUniRule annotation
Ordered Locus Names:NE0785
OrganismiNitrosomonas europaea (strain ATCC 19718 / CIP 103999 / KCTC 2705 / NBRC 14298)
Taxonomic identifieri228410 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaNitrosomonadalesNitrosomonadaceaeNitrosomonas
Proteomesi
  • UP000001416 Componenti: Chromosome

Subcellular locationi

Q82WA9:
  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002273341 – 695UvrABC system protein BAdd BLAST695

Interactioni

Subunit structurei

Forms a heterotetramer with UvrA during the search for lesions. Interacts with UvrC in an incision complex.UniRule annotation

Protein-protein interaction databases

STRINGi228410.NE0785.

Structurei

3D structure databases

ProteinModelPortaliQ82WA9.
SMRiQ82WA9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini31 – 414Helicase ATP-bindingUniRule annotationAdd BLAST384
Domaini435 – 601Helicase C-terminalUniRule annotationAdd BLAST167
Domaini632 – 667UVRUniRule annotationAdd BLAST36

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi97 – 120Beta-hairpinAdd BLAST24

Domaini

The beta-hairpin motif is involved in DNA binding.UniRule annotation

Sequence similaritiesi

Belongs to the UvrB family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CCW. Bacteria.
COG0556. LUCA.
HOGENOMiHOG000073580.
KOiK03702.
OMAiRYMHSEI.
OrthoDBiPOG091H02LK.
PhylomeDBiQ82WA9.

Family and domain databases

CDDicd00079. HELICc. 1 hit.
Gene3Di4.10.860.10. 1 hit.
HAMAPiMF_00204. UvrB. 1 hit.
InterProiView protein in InterPro
IPR006935. Helicase/UvrB_N.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR001943. UVR_dom.
IPR036876. UVR_dom_sf.
IPR004807. UvrB.
IPR024759. UvrB_YAD/RRR_dom.
PANTHERiPTHR24029. PTHR24029. 1 hit.
PfamiView protein in Pfam
PF00271. Helicase_C. 1 hit.
PF04851. ResIII. 1 hit.
PF02151. UVR. 1 hit.
PF12344. UvrB. 1 hit.
SMARTiView protein in SMART
SM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
SUPFAMiSSF46600. SSF46600. 1 hit.
SSF52540. SSF52540. 3 hits.
TIGRFAMsiTIGR00631. uvrb. 1 hit.
PROSITEiView protein in PROSITE
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS50151. UVR. 1 hit.

Sequencei

Sequence statusi: Complete.

Q82WA9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIITFPGSPY KLNQAFQPAG DQPEAIRILV EGIESGLSFQ TLLGVTGSGK
60 70 80 90 100
TFTIANMIAR LGRPAIIMAP NKTLAAQLYA EMREFFPENA VEYFVSYYDY
110 120 130 140 150
YQPEAYVPSR DLFIEKDSSI NEHIEQMRLS ATKSLLERED AIIVATVSCI
160 170 180 190 200
YGIGDPVDYH GMILHVREHE KISQRDIIQR LTGMQYQRNE FEFARGTFRV
210 220 230 240 250
RGDVLDVFPA ENSETALRIS LFDDEVESMT LFDPLTGQTR QKVSRYTVYP
260 270 280 290 300
SSHYVTPRST TLRAIETIKT ELTGRLNYFH ENHKLVEAQR LEQRTRFDLE
310 320 330 340 350
MLNELGFCKG IENYSRHLSG RQPGDPPPTL IDYLPDNALM IIDESHVTVP
360 370 380 390 400
QIGGMYKGDR SRKENLVAYG FRLPSALDNR PLRFEEFEKL MPQTIFVSAT
410 420 430 440 450
PADYEIQRSG QIAEQVVRPT GLVDPVIIIR PVTTQVDDLM SEVSLRAAQN
460 470 480 490 500
ERVLVTTLTK RMAEDLTDYF SDHGIRVRYL HSDIDTVERV EIIRDLRLGK
510 520 530 540 550
FDVLVGINLL REGLDIPEVS LVGILDADKE GFLRSERSLI QTMGRAARHV
560 570 580 590 600
NGTVILYADK ITNSMRRAID ETERRRNKQK LFNQQNNITP RGVNKRIKDL
610 620 630 640 650
IDGVYDSENA AEHRKVAQIQ ARYAAMDEAQ LAKEIQRLEK SMLEAARNME
660 670 680 690
FEQAAQYRDE IKNLRSKLFI GIIDPDEIRE VPQTAGKKSR RKAGR
Length:695
Mass (Da):79,423
Last modified:June 1, 2003 - v1
Checksum:i62BD4D26765A0251
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL954747 Genomic DNA. Translation: CAD84696.1.
RefSeqiWP_011111397.1. NC_004757.1.

Genome annotation databases

EnsemblBacteriaiCAD84696; CAD84696; NE0785.
KEGGineu:NE0785.

Similar proteinsi

Entry informationi

Entry nameiUVRB_NITEU
AccessioniPrimary (citable) accession number: Q82WA9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 7, 2006
Last sequence update: June 1, 2003
Last modified: October 25, 2017
This is version 91 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families