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Q82VS0 (DDL_NITEU) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 77. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase

EC=6.3.2.4
Alternative name(s):
D-Ala-D-Ala ligase
D-alanylalanine synthetase
Gene names
Name:ddl
Synonyms:ddlB
Ordered Locus Names:NE0994
OrganismNitrosomonas europaea (strain ATCC 19718 / NBRC 14298) [Complete proteome] [HAMAP]
Taxonomic identifier228410 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaNitrosomonadalesNitrosomonadaceaeNitrosomonas

Protein attributes

Sequence length305 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 305305D-alanine--D-alanine ligase HAMAP-Rule MF_00047
PRO_0000177847

Regions

Domain105 – 300196ATP-grasp
Nucleotide binding131 – 18656ATP By similarity

Sites

Metal binding2541Magnesium or manganese 1 By similarity
Metal binding2671Magnesium or manganese 1 By similarity
Metal binding2671Magnesium or manganese 2 By similarity
Metal binding2691Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q82VS0 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: 54B1F11950432305

FASTA30533,442
        10         20         30         40         50         60 
MNTRDVGKVA VLLGGRSAER EISLRSGQAV LAALQRSRVN AHAFDPAGQP LENLLQQGFD 

        70         80         90        100        110        120 
RVFIALHGRY GEDGSVQGAL ELMELPYTGS GILASALAMD KWRTKMIWQA AGINTPDYVM 

       130        140        150        160        170        180 
LDASSRFRDV ADRLGLPLII KPAREGSTLG LNKVDNEQDF RSAYQAAAEY DSLVLAEQFI 

       190        200        210        220        230        240 
QGIELTAAIL DDMPLPLVRI DVAEGLYDYQ AKYFSESTRY TCPSGLSAAL TTRIQEQALY 

       250        260        270        280        290        300 
AHRILGCTGW SRVDLILDEN EQPFFLETNT SPGMTDHSLV PMAAKAAGIS FDELVVQILE 


LSCEH 

« Hide

References

[1]"Complete genome sequence of the ammonia-oxidizing bacterium and obligate chemolithoautotroph Nitrosomonas europaea."
Chain P., Lamerdin J.E., Larimer F.W., Regala W., Lao V., Land M.L., Hauser L., Hooper A.B., Klotz M.G., Norton J., Sayavedra-Soto L.A., Arciero D.M., Hommes N.G., Whittaker M.M., Arp D.J.
J. Bacteriol. 185:2759-2773(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 19718 / NBRC 14298.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AL954747 Genomic DNA. Translation: CAD84905.1.
RefSeqNP_841067.1. NC_004757.1.

3D structure databases

ProteinModelPortalQ82VS0.
SMRQ82VS0. Positions 7-302.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING228410.NE0994.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAD84905; CAD84905; NE0994.
GeneID1081936.
KEGGneu:NE0994.
PATRIC22713008. VBINitEur56163_1105.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1181.
HOGENOMHOG000011592.
KOK01921.
OMACSGYSRV.
OrthoDBEOG6ND0KB.
PhylomeDBQ82VS0.

Enzyme and pathway databases

BioCycNEUR228410:GJNO-1017-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 2 hits.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDDL_NITEU
AccessionPrimary (citable) accession number: Q82VS0
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 2003
Last sequence update: June 1, 2003
Last modified: July 9, 2014
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways