Q82VD1 (GCH4_NITEU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
GTP cyclohydrolase folE2
EC=3.5.4.16

Gene names

Name:	folE2
Ordered Locus Names:	NE1163

Organism

Nitrosomonas europaea (strain ATCC 19718 / NBRC 14298) [Complete proteome] [HAMAP]

Taxonomic identifier

228410 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Betaproteobacteria › Nitrosomonadales › Nitrosomonadaceae › Nitrosomonas › Nitrosomonas europaea

Protein attributes

Sequence length	268 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Converts GTP to 7,8-dihydroneopterin triphosphate By similarity. HAMAP-Rule MF_01527
Catalytic activity	GTP + H2O = formate + 2-amino-4-hydroxy-6-(erythro-1,2,3-trihydroxypropyl)-dihydropteridine triphosphate. HAMAP-Rule MF_01527
Pathway	Cofactor biosynthesis; 7,8-dihydroneopterin triphosphate biosynthesis; 7,8-dihydroneopterin triphosphate from GTP: step 1/1. HAMAP-Rule MF_01527
Sequence similarities	Belongs to the GTP cyclohydrolase IV family.

Ontologies

Keywords
Molecular function	Hydrolase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological_process	7,8-dihydroneopterin 3'-triphosphate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	GTP cyclohydrolase I activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 268

268

GTP cyclohydrolase folE2 HAMAP-Rule MF_01527

PRO_0000147717

Sites

Site

154

1

May be catalytically important By similarity

Secondary structure

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268

Helix Strand Turn

Details...

Beta strand

24 – 37

14

Turn

40 – 42

3

Beta strand

45 – 57

13

Helix

63 – 67

5

Helix

68 – 75

8

Beta strand

78 – 81

4

Helix

82 – 84

3

Helix

85 – 95

11

Beta strand

99 – 114

16

Turn

116 – 118

3

Beta strand

121 – 135

15

Turn

136 – 138

3

Beta strand

139 – 153

15

Helix

155 – 160

6

Beta strand

161 – 163

3

Beta strand

167 – 180

14

Helix

184 – 194

11

Beta strand

195 – 198

4

Helix

205 – 216

12

Helix

222 – 234

13

Beta strand

239 – 249

11

Turn

251 – 253

3

Beta strand

254 – 264

11

Sequences

	Sequence		Length	Mass (Da)
	Q82VD1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: DAA9B16495ED23E4	FASTA	268	30,604
	10 20 30 40 50 60 MNKQIDLPIA DVQGSLDTRH IAIDRVGIKA IRHPVVVADK GGGSQHTVAQ FNMYVNLPHN 70 80 90 100 110 120 FKGTHMSRFV EILNSHEREI SVESFEEILR SMVSRLESDS GHIEMAFPYF INKSAPVSGV 130 140 150 160 170 180 KSLLDYEVTF IGEIKHGNQY SFTMKVIVPV TSLCPCSKKI SDYGAHNQRS HVTISVRTNS 190 200 210 220 230 240 FIWIEDIIRI AEEQASCELY GLLKRPDEKY VTERAYNNPK FVEDIVRDVA EVLNHDDRID 250 260 AYIVESENFE SIHNHSAYAL IERDKRIR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete genome sequence of the ammonia-oxidizing bacterium and obligate chemolithoautotroph Nitrosomonas europaea." Chain P., Lamerdin J.E., Larimer F.W., Regala W., Lao V., Land M.L., Hauser L., Hooper A.B., Klotz M.G., Norton J., Sayavedra-Soto L.A., Arciero D.M., Hommes N.G., Whittaker M.M., Arp D.J. J. Bacteriol. 185:2759-2773(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 19718 / NBRC 14298.
[2]	"The crystal structure of duf198 from Nitrosomonas europaea ATCC 19718." Midwest center for structural genomics (MCSG) Submitted (SEP-2007) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (3.05 ANGSTROMS).

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AL954747 Genomic DNA. Translation: CAD85074.1.

RefSeq

NP_841220.1. NC_004757.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2R5R	X-ray	3.05	A	1-268	[»]

ProteinModelPortal

Q82VD1.

SMR

Q82VD1. Positions 20-265.

ModBase

Search...

Protein-protein interaction databases

STRING

228410.NE1163.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

CAD85074; CAD85074; NE1163.

GeneID

1082107.

KEGG

neu:NE1163.

PATRIC

22713382. VBINitEur56163_1290.

Organism-specific databases

CMR

Search...

Phylogenomic databases

eggNOG

COG1469.

HOGENOM

HOG000280679.

KO

K09007.

OMA

INMYVDL.

ProtClustDB

PRK13674.

Enzyme and pathway databases

BioCyc

NEUR228410:GJNO-1188-MONOMER.

UniPathway

UPA00848; UER00151.

Family and domain databases

Gene3D

3.10.270.10. 1 hit.

HAMAP

MF_01527_B. GTP_cyclohydrol_B.

InterPro

IPR022838. GTP_cyclohydrolase_FolE2.
IPR003801. GTP_cyclohydrolase_FolE2/MptA.
IPR002042. Uricase.
[Graphical view]

Pfam

PF02649. GCHY-1. 1 hit.
[Graphical view]

TIGRFAMs

TIGR00294. TIGR00294. 1 hit.

ProtoNet

Search...

Other

EvolutionaryTrace

Q82VD1.

Entry information

Entry name

GCH4_NITEU

Accession

Primary (citable) accession number: Q82VD1

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 2005
Last sequence update:	June 1, 2003
Last modified:	May 1, 2013
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program