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Protein

Argininosuccinate synthase

Gene

argG

Organism
Nitrosomonas europaea (strain ATCC 19718 / CIP 103999 / KCTC 2705 / NBRC 14298)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate.UniRule annotation

Pathwayi: L-arginine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate.UniRule annotation
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Ornithine carbamoyltransferase (argF)
  2. Argininosuccinate synthase (argG)
  3. Argininosuccinate lyase (argH)
This subpathway is part of the pathway L-arginine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate, the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei37ATP; via amide nitrogen and carbonyl oxygenUniRule annotation1
Binding sitei88CitrullineUniRule annotation1
Binding sitei93CitrullineUniRule annotation1
Binding sitei118ATP; via amide nitrogenUniRule annotation1
Binding sitei120AspartateUniRule annotation1
Binding sitei124AspartateUniRule annotation1
Binding sitei124CitrullineUniRule annotation1
Binding sitei125AspartateUniRule annotation1
Binding sitei128CitrullineUniRule annotation1
Binding sitei179CitrullineUniRule annotation1
Binding sitei188CitrullineUniRule annotation1
Binding sitei264CitrullineUniRule annotation1
Binding sitei276CitrullineUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi10 – 18ATPUniRule annotation9

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Amino-acid biosynthesis, Arginine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00068; UER00113.

Names & Taxonomyi

Protein namesi
Recommended name:
Argininosuccinate synthaseUniRule annotation (EC:6.3.4.5UniRule annotation)
Alternative name(s):
Citrulline--aspartate ligaseUniRule annotation
Gene namesi
Name:argGUniRule annotation
Ordered Locus Names:NE1437
OrganismiNitrosomonas europaea (strain ATCC 19718 / CIP 103999 / KCTC 2705 / NBRC 14298)
Taxonomic identifieri228410 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaNitrosomonadalesNitrosomonadaceaeNitrosomonas
Proteomesi
  • UP000001416 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001486181 – 404Argininosuccinate synthaseAdd BLAST404

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi228410.NE1437.

Structurei

3D structure databases

ProteinModelPortaliQ82UP5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the argininosuccinate synthase family. Type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CDH. Bacteria.
COG0137. LUCA.
HOGENOMiHOG000230093.
KOiK01940.
OMAiQCEVVTF.
OrthoDBiPOG091H01U0.
PhylomeDBiQ82UP5.

Family and domain databases

CDDicd01999. Argininosuccinate_Synthase. 1 hit.
Gene3Di3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPiMF_00005. Arg_succ_synth_type1. 1 hit.
InterProiIPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00032. argG. 1 hit.
PROSITEiPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q82UP5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDKVKKAVLA FSGGLDTSVI LKWLQDTYQC EVVTFTADIG QGEEIEPARA
60 70 80 90 100
KAVQFGIREI FIEDLREEFV RDYVFPMFRA NTIYEGEYLL GTSIARPLIA
110 120 130 140 150
KRQVEIAQQT GADAVSHGAT GKGNDQVRFE LGYYALQPDI RVIAPWREWD
160 170 180 190 200
LTSREKLLTY AEKQGIPIEM KQKAGSPYSM DANLLHISYE GRALEDPAAE
210 220 230 240 250
PEESMWRWTV SPETAPSEPE YLDLEYERGD IVALNGERLS PAAILTRLNQ
260 270 280 290 300
LGGKHGIGRL DLVENRYVGM KSRGCYETPG GTIMLRAHRA IESITLDREV
310 320 330 340 350
AHLKDDLMPR YAALIYNGYW WSPERKLLQV LIDESQVNVN GRVRVKLYKG
360 370 380 390 400
NVMVVGRDSR TDSLFDPDIA TFEEDGGAYH QADAAGFIKL NALRMRIAKA

LRRC
Length:404
Mass (Da):45,614
Last modified:June 1, 2003 - v1
Checksum:i521463E865D5B724
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL954747 Genomic DNA. Translation: CAD85348.1.
RefSeqiWP_011112005.1. NC_004757.1.

Genome annotation databases

EnsemblBacteriaiCAD85348; CAD85348; NE1437.
KEGGineu:NE1437.
PATRICi22714043. VBINitEur56163_1615.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL954747 Genomic DNA. Translation: CAD85348.1.
RefSeqiWP_011112005.1. NC_004757.1.

3D structure databases

ProteinModelPortaliQ82UP5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi228410.NE1437.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAD85348; CAD85348; NE1437.
KEGGineu:NE1437.
PATRICi22714043. VBINitEur56163_1615.

Phylogenomic databases

eggNOGiENOG4105CDH. Bacteria.
COG0137. LUCA.
HOGENOMiHOG000230093.
KOiK01940.
OMAiQCEVVTF.
OrthoDBiPOG091H01U0.
PhylomeDBiQ82UP5.

Enzyme and pathway databases

UniPathwayiUPA00068; UER00113.

Family and domain databases

CDDicd01999. Argininosuccinate_Synthase. 1 hit.
Gene3Di3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPiMF_00005. Arg_succ_synth_type1. 1 hit.
InterProiIPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00032. argG. 1 hit.
PROSITEiPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiASSY_NITEU
AccessioniPrimary (citable) accession number: Q82UP5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 19, 2003
Last sequence update: June 1, 2003
Last modified: November 2, 2016
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.