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Q82TL0 (HEM6_NITEU) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 53. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Coproporphyrinogen-III oxidase, aerobic
Short name=Coprogen oxidase
Short name=Coproporphyrinogenase
EC=1.3.3.3
Gene names
Name:hemF
Ordered Locus Names:NE1876
OrganismNitrosomonas europaea [Complete proteome] [HAMAP]
Taxonomic identifier915 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Betaproteobacteria › Nitrosomonadales › Nitrosomonadaceae › Nitrosomonas

Protein attributes

Sequence length301 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Key enzyme in heme biosynthesis. Catalyzes the oxidative decarboxylation of propionic acid side chains of rings A and B of coproporphyrinogen III By similarity. HAMAP MF_00333

Catalytic activity

Coproporphyrinogen-III + O2 + 2 H+ = protoporphyrinogen-IX + 2 CO2 + 2 H2O. HAMAP MF_00333

Pathway

Porphyrin metabolism; protoporphyrin-IX biosynthesis; protoporphyrinogen-IX from coproporphyrinogen-III (O2 route): step 1/1. HAMAP MF_00333

Subunit structure

Homodimer By similarity. HAMAP MF_00333

Subcellular location

Cytoplasm By similarity HAMAP MF_00333.

Sequence similarities

Belongs to the aerobic coproporphyrinogen-III oxidase family.

Ontologies

Keywords
   Biological processPorphyrin biosynthesis
   Cellular componentCytoplasm
   Molecular functionOxidoreductase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processporphyrin-containing compound biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functioncoproporphyrinogen oxidase activity

Inferred from electronic annotation. Source: EC

protein homodimerization activity

Inferred from sequence or structural similarity. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 301301Coproporphyrinogen-III oxidase, aerobic HAMAP MF_00333
PRO_0000109904

Regions

Region46 – 5510Important for dimerization By similarity
Region106 – 1083Substrate binding By similarity
Region238 – 27336Important for dimerization By similarity
Region256 – 2616Substrate binding By similarity

Sites

Active site1041Proton donor By similarity
Binding site901Substrate By similarity
Site1731Important for dimerization By similarity

Sequences

Sequence LengthMass (Da)Tools
Q82TL0 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: 5EAFE60323774641

FASTA30134,157
        10         20         30         40         50         60 
MNFAQVKDYL VDLQNRIVTG LEQVDGQSFR RDTWDRPEGG GGTSCVIEEG NVLERGGVNF 

        70         80         90        100        110        120 
SHVFGKGLPA SATAARPELA GRAFEAAGVS LVLHPRNPYA PTVHMNVRFF AATKEGAEPV 

       130        140        150        160        170        180 
WWFGGGMDLT PYYGFEEDAV HFHQACKDAL QPSGEEYYPR FKKWCDEYFY LKHRKEPRGI 

       190        200        210        220        230        240 
GGVFFDDLNQ PDFATCFNLT RSVGDHFLAA YVPILQKRRD LPYGERERDF QAYRRGRYVE 

       250        260        270        280        290        300 
FNLVWDRGTL FGLQSGGRTE SILMSLPPVV KWRYDWSPAA GSPEAKLYTD FLTGRDWLPL 


G

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References

[1]"Complete genome sequence of the ammonia-oxidizing bacterium and obligate chemolithoautotroph Nitrosomonas europaea."
Chain P., Lamerdin J.E., Larimer F.W., Regala W., Lao V., Land M.L., Hauser L., Hooper A.B., Klotz M.G., Norton J., Sayavedra-Soto L.A., Arciero D.M., Hommes N.G., Whittaker M.M., Arp D.J.
J. Bacteriol. 185:2759-2773(2003) [PubMed: 12700255] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 19718 / NBRC 14298.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AL954747 Genomic DNA. Translation: CAD85787.1.
RefSeqNP_841898.1. NC_004757.1.

3D structure databases

ProteinModelPortalQ82TL0.
SMRQ82TL0. Positions 1-298.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID1082833.
GenomeReviewsGene locus NE1876 in contig AL954747_GR.
KEGGneu:NE1876.
NMPDRfig|228410.1.peg.1797.
PATRIC22715029. VBINitEur56163_2101.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG631180.
OMAVKAYLLD.
ProtClustDBPRK05330.

Enzyme and pathway databases

BioCycNEUR228410:NE1876-MONOMER.

Family and domain databases

HAMAPMF_00333. Coprogen_oxidas.
[Tree]
InterProIPR001260. Coprogen_oxidase_aer.
IPR018375. Coprogen_oxidase_CS.
[Graphical view]
Gene3DG3DSA:3.40.1500.10. Coprogen_oxidas. 1 hit.
KOK00228.
PANTHERPTHR10755. Coprogen_oxidas. 1 hit.
PfamPF01218. Coprogen_oxidas. 1 hit.
[Graphical view]
PIRSFPIRSF000166. Coproporphyri_ox. 1 hit.
PRINTSPR00073. COPRGNOXDASE.
SUPFAMSSF102886. Coprogen_oxidas. 1 hit.
PROSITEPS01021. COPROGEN_OXIDASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameHEM6_NITEU
AccessionPrimary (citable) accession number: Q82TL0
Entry history
Integrated into UniProtKB/Swiss-Prot: January 16, 2004
Last sequence update: June 1, 2003
Last modified: January 25, 2012
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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