Reviewed, UniProtKB/Swiss-Prot Q82TF3 (HPPA_NITEU)

Names and origin

Protein names

Recommended name:
    Pyrophosphate-energized proton pump
    EC=3.6.1.1
Alternative name(s):
    Pyrophosphate-energized inorganic pyrophosphatase
      Short name=H(+)-PPase
    Membrane-bound proton-translocating pyrophosphatase

Gene names

Name:	hppA
Ordered Locus Names:	NE1935

Organism

Nitrosomonas europaea [Complete proteome] [HAMAP]

Taxonomic identifier

915 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Nitrosomonadales › Nitrosomonadaceae › Nitrosomonas

Protein attributes

Sequence length	685 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Generates a proton motive force; it probably catalyzes a fully reversible reaction, thus being able to synthesize pyrophosphate when the proton motive force is sufficient By similarity.
Catalytic activity	Diphosphate + H2O = 2 phosphate. HAMAP MF_01130
Cofactor	Magnesium By similarity.
Subunit structure	Homodimer Potential.
Subcellular location	Cell membrane; Multi-pass membrane protein Probable.
Sequence similarities	Belongs to the H(+)-translocating pyrophosphatase (TC 3.A.10) family. Type 2 subfamily. [View classification]

Ontologies

Keywords
Biological process	Hydrogen ion transport Ion transport Transport
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Ligand	Magnesium
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proton transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: HAMAP
Molecular function	hydrogen-translocating pyrophosphatase activity Inferred from electronic annotation. Source: InterPro inorganic diphosphatase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 685	685	Pyrophosphate-energized proton pump HAMAP MF_01130		PRO_0000217022
Regions
	Transmembrane	5 – 27	23	Potential
	Transmembrane	55 – 74	20	Potential
	Transmembrane	78 – 100	23	Potential
	Transmembrane	119 – 141	23	Potential
	Transmembrane	161 – 183	23	Potential
	Transmembrane	232 – 250	19	Potential
	Transmembrane	260 – 282	23	Potential
	Transmembrane	295 – 317	23	Potential
	Transmembrane	332 – 354	23	Potential
	Transmembrane	383 – 405	23	Potential
	Transmembrane	409 – 431	23	Potential
	Transmembrane	474 – 491	18	Potential
	Transmembrane	506 – 528	23	Potential
	Transmembrane	578 – 600	23	Potential
	Transmembrane	604 – 626	23	Potential
Sites
	Site	469	1	Determinant of potassium independence By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q82TF3-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: C196BDC99CF88507	FASTA	685	70,050
	10 20 30 40 50 60 MANGLTIAIC SAILALIFSG LWIRRIYAQS AGDSRMQEIA AAVQEGASAY LKRQYLTIGM 70 80 90 100 110 120 VGTVLFVIIG LALSWNTAIG FALGAILSGL AGFMGMNVSV QSNVRTAEAA RSGLNEALAI 130 140 150 160 170 180 AFRGGAVTGM LVVGLGLLGV AGYTALLVSG ADETSSISDL IHPLIGFAFG GSLISIFARL 190 200 210 220 230 240 GGGIFTKGAD VGADLVGKVE AGIPEDDPRN PAVIADNVGD NVGDCAGMAA DLFETYAVTI 250 260 270 280 290 300 IATMLLGALL FKTGTGDAAV YPLALGAASI VASIIGCYFV KMREGGKIMN ALYRGLAVAG 310 320 330 340 350 360 GIAFFAYLPI TVWFMGGATL TLDGTEVGGG ELIMRLFAST TIGLVLTGLM VVITEYYTST 370 380 390 400 410 420 EYPPVQHIAN ASTTGHATNI IAGLGVGMRA TAAPVLAVCA SIIVAYSLAG LYGIAIAATA 430 440 450 460 470 480 MLSMTGIIVA LDAYGPITDN AGGIAEMAGM PESVRAVTDP LDAVGNTTKA VTKGYAIGSA 490 500 510 520 530 540 GLAALVLFAD YTHGLEHANK LMTFDLSNHL VIIGLFIGGM VPFLFGAMSM EAVGRAAGSV 550 560 570 580 590 600 VLEVRRQFKE IPGIMDGSRK PDYSRAVDML TKAAIREMIV PSLLPVLIPV LVGVFLGPQA 610 620 630 640 650 660 LGGVLMGSIV TGLFIAISMT AGGGAWDNAK KYIEDGNYGG KGSDAHKAAV TGDTVGDPYK 670 680 DTAGPAINPL IKIINIVALL IIPLL

References

[1]

"Complete genome sequence of the ammonia-oxidizing bacterium and obligate chemolithoautotroph Nitrosomonas europaea." Chain P., Lamerdin J.E., Larimer F.W., Regala W., Lao V., Land M.L., Hauser L., Hooper A.B., Klotz M.G., Norton J., Sayavedra-Soto L.A., Arciero D.M., Hommes N.G., Whittaker M.M., Arp D.J. J. Bacteriol. 185:2759-2773(2003) [PubMed: 12700255] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 19718 / IFO 14298.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL954747 Genomic DNA. Translation: CAD85846.1.
RefSeq	NP_841957.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1082893.
GenomeReviews	Gene locus NE1935 in contig AL954747_GR.
KEGG	neu:NE1935.
NMPDR	fig|228410.1.peg.1856.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q82TF3.
OMA	VFSAMAM.
Enzyme and pathway databases
BioCyc	NEUR228410:NE1935-MON.
BRENDA	3.6.1.1. 444.
Family and domain databases
HAMAP	MF_01130. [Tree]
InterPro	IPR004131. H_PPase. [Graphical view]
Pfam	PF03030. H_PPase. 1 hit. [Graphical view]
PIRSF	PIRSF001265. H+-PPase. 1 hit.
TIGRFAMs	TIGR01104. V_PPase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HPPA_NITEU

Accession

Primary (citable) accession number: Q82TF3

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2003
Last sequence update:	June 1, 2003
Last modified:	November 3, 2009
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)