Reviewed, UniProtKB/Swiss-Prot Q82TC8 (DEF2_NITEU)

Names and origin

Protein names

Recommended name:
    Peptide deformylase 2
      Short name=PDF 2
    EC=3.5.1.88
Alternative name(s):
    Polypeptide deformylase 2

Gene names

Name:	def2
Ordered Locus Names:	NE1970

Organism

Nitrosomonas europaea [Complete proteome] [HAMAP]

Taxonomic identifier

915 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Nitrosomonadales › Nitrosomonadaceae › Nitrosomonas

Protein attributes

Sequence length	185 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions By similarity.
Catalytic activity	Formyl-L-methionyl peptide + H2O = formate + methionyl peptide. HAMAP MF_00163
Cofactor	Binds 1 Fe2+ ion By similarity.
Sequence similarities	Belongs to the polypeptide deformylase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Ligand	Iron Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	translation Inferred from electronic annotation. Source: HAMAP
Molecular function	iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW peptide deformylase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 185	185	Peptide deformylase 2 HAMAP MF_00163		PRO_0000082810
Sites
	Active site	151	1	By similarity
	Metal binding	108	1	Iron By similarity
	Metal binding	150	1	Iron By similarity
	Metal binding	154	1	Iron By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q82TC8-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: C8E1A641426FDEC1	FASTA	185	20,573
	10 20 30 40 50 60 MIEPLPRILV SELCKFVMAI LNILRYPDER LHKIATEVPS ITREIRTLVS NMAETMYAAP 70 80 90 100 110 120 GIGLAATQVD VHQRIIVIDV SETRDELLVL INPEIIASSG NAETQEGCLS VPGIFDKVTR 130 140 150 160 170 180 AEEVTVRATG IDGKSFEMDA SGLLAVCIQH EMDHLMGKVF VEYLSPFKQS RILSKLKKQA RRQIA

References

[1]

"Complete genome sequence of the ammonia-oxidizing bacterium and obligate chemolithoautotroph Nitrosomonas europaea." Chain P., Lamerdin J.E., Larimer F.W., Regala W., Lao V., Land M.L., Hauser L., Hooper A.B., Klotz M.G., Norton J., Sayavedra-Soto L.A., Arciero D.M., Hommes N.G., Whittaker M.M., Arp D.J. J. Bacteriol. 185:2759-2773(2003) [PubMed: 12700255] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 19718 / IFO 14298.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL954747 Genomic DNA. Translation: CAD85881.1.
RefSeq	NP_841987.1.
3D structure databases
HSSP	HSSP built from PDB template 2DEF based on UniProtKB P27251.
ModBase	Search...
Genome annotation databases
GeneID	1082928.
GenomeReviews	Gene locus NE1970 in contig AL954747_GR.
KEGG	neu:NE1970.
NMPDR	fig|228410.1.peg.1886.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q82TC8.
OMA	CIQHECD.
Enzyme and pathway databases
BioCyc	NEUR228410:NE1970-MON.
BRENDA	3.5.1.88. 444.
Family and domain databases
HAMAP	MF_00163. [Tree]
InterPro	IPR000181. Fmet_deformylase. [Graphical view]
Gene3D	G3DSA:3.90.45.10. Fmet_deformylase. 1 hit.
PANTHER	PTHR10458. Fmet_deformylase. 1 hit.
Pfam	PF01327. Pep_deformylase. 1 hit. [Graphical view]
PIRSF	PIRSF004749. Pep_def. 1 hit.
PRINTS	PR01576. PDEFORMYLASE.
ProDom	PD003844. Fmet_deformylase. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00079. pept_deformyl. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DEF2_NITEU

Accession

Primary (citable) accession number: Q82TC8

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 2003
Last sequence update:	June 1, 2003
Last modified:	November 3, 2009
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)