Reviewed, UniProtKB/Swiss-Prot Q82SM5 (FUMC_NITEU)

Names and origin

Protein names

Recommended name:
    Fumarate hydratase class II
      Short name=Fumarase C
    EC=4.2.1.2

Gene names

Name:	fumC
Ordered Locus Names:	NE2286

Organism

Nitrosomonas europaea [Complete proteome] [HAMAP]

Taxonomic identifier

915 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Nitrosomonadales › Nitrosomonadaceae › Nitrosomonas

Protein attributes

Sequence length	462 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-malate = fumarate + H2O. HAMAP MF_00743
Pathway	Carbohydrate metabolism; tricarboxylic acid cycle. HAMAP MF_00743
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm By similarity.
Miscellaneous	There are 2 substrate binding sites: the catalytic A site, and the non-catalytic B site that may play a role in the transfer of substrate or product between the active site and the solvent. Alternatively, the B site may bind allosteric effectors By similarity.
Sequence similarities	Belongs to the class-II fumarase/aspartase family. Fumarase subfamily.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Cellular component	Cytoplasm
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	fumarate metabolic process Inferred from electronic annotation. Source: InterPro tricarboxylic acid cycle Inferred from electronic annotation. Source: HAMAP
Cellular component	tricarboxylic acid cycle enzyme complex Inferred from electronic annotation. Source: InterPro
Molecular function	fumarate hydratase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 462	462	Fumarate hydratase class II HAMAP MF_00743		PRO_0000161292
Regions
	Region	129 – 132	4	B site By similarity
	Region	139 – 141	3	Substrate binding By similarity
Sites
	Binding site	100	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q82SM5-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: D20178003603A48A	FASTA	462	49,530
	10 20 30 40 50 60 MDQYREEHDA IGTVQVPASA LWGAQTQRSL NNFNISGERM PSALIHALAL VKRAAASVNH 70 80 90 100 110 120 DLGLLDENIA RAIITAADEV LAGEHAGEFP LVVWQTGSGT QTNMNMNEVL ANRASEILGG 130 140 150 160 170 180 TRGKGRKVHP NDHVNKGQSS NDVFPTAMHI AAVEAIRNRL IPALEALRKT LSSKSAAFSD 190 200 210 220 230 240 IVKIGRTHLQ DATPLTLGQE FSGYVSQLDH GLAHLESALP HLLELALGGT AVGTGLNTHP 250 260 270 280 290 300 EFARRVAAEI ARLSGYPFIT AANKFEALAA HDALVHAHGV LKTLAAILIK IANDVRWLAS 310 320 330 340 350 360 GPRCGIGEIL IPENEPGSSI MPGKVNPTQS EAVVMLACQV MGNDVAINLG GAMGNFELNT 370 380 390 400 410 420 MKPLIIHNFL QSTRLLADGA ESFNTHCAAG ITANTVRIKQ HLQESLMLVT ALNPHIGYDK 430 440 450 460 AAEIAKKAHH EMLTLKEAAI RLGYVTAEQF DVWVDPQKMT EV

References

[1]

"Complete genome sequence of the ammonia-oxidizing bacterium and obligate chemolithoautotroph Nitrosomonas europaea." Chain P., Lamerdin J.E., Larimer F.W., Regala W., Lao V., Land M.L., Hauser L., Hooper A.B., Klotz M.G., Norton J., Sayavedra-Soto L.A., Arciero D.M., Hommes N.G., Whittaker M.M., Arp D.J. J. Bacteriol. 185:2759-2773(2003) [PubMed: 12700255] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 19718 / IFO 14298.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL954747 Genomic DNA. Translation: CAD86198.1.
RefSeq	NP_842286.1.
3D structure databases
HSSP	HSSP built from PDB template 1FUR based on UniProtKB P05042.
SMR	Q82SM5. Positions 5-459.
ModBase	Search...
Genome annotation databases
GeneID	1083253.
GenomeReviews	Gene locus NE2286 in contig AL954747_GR.
KEGG	neu:NE2286.
NMPDR	fig|228410.1.peg.2185.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q82SM5.
OMA	GSQGHFE.
Enzyme and pathway databases
BioCyc	NEUR228410:NE2286-MON.
BRENDA	4.2.1.2. 444.
Family and domain databases
HAMAP	MF_00743. [Tree]
InterPro	IPR003031. D_crystallin. IPR005677. Fum_hydII. IPR018951. Fumarase_C_C. IPR000362. Fumarate_lyase. IPR020557. Fumarate_lyase_CS. [Graphical view]
Pfam	PF10415. FumaraseC_C. 1 hit. PF00206. Lyase_1. 1 hit. [Graphical view]
PRINTS	PR00145. ARGSUCLYASE. PR00149. FUMRATELYASE.
TIGRFAMs	TIGR00979. fumC_II. 1 hit.
PROSITE	PS00163. FUMARATE_LYASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FUMC_NITEU

Accession

Primary (citable) accession number: Q82SM5

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 2003
Last sequence update:	June 1, 2003
Last modified:	November 3, 2009
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)