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Q82SL7

- BIOB_NITEU

UniProt

Q82SL7 - BIOB_NITEU

Protein

Biotin synthase

Gene

bioB

Organism
Nitrosomonas europaea (strain ATCC 19718 / NBRC 14298)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 77 (01 Oct 2014)
      Sequence version 2 (28 Jul 2009)
      Previous versions | rss
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    Functioni

    Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.UniRule annotation

    Catalytic activityi

    Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

    Cofactori

    Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation
    Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi77 – 771Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi81 – 811Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi84 – 841Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi121 – 1211Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi152 – 1521Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi212 – 2121Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi284 – 2841Iron-sulfur 2 (2Fe-2S)UniRule annotation

    GO - Molecular functioni

    1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
    2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    3. biotin synthase activity Source: UniProtKB-HAMAP
    4. iron ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. biotin biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Biotin biosynthesis

    Keywords - Ligandi

    2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciNEUR228410:GJNO-2345-MONOMER.
    UniPathwayiUPA00078; UER00162.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Biotin synthaseUniRule annotation (EC:2.8.1.6UniRule annotation)
    Gene namesi
    Name:bioBUniRule annotation
    Ordered Locus Names:NE2300
    OrganismiNitrosomonas europaea (strain ATCC 19718 / NBRC 14298)
    Taxonomic identifieri228410 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaNitrosomonadalesNitrosomonadaceaeNitrosomonas
    ProteomesiUP000001416: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 337337Biotin synthasePRO_0000381501Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi228410.NE2300.

    Structurei

    3D structure databases

    ProteinModelPortaliQ82SL7.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the radical SAM superfamily. Biotin synthase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0502.
    HOGENOMiHOG000239957.
    KOiK01012.
    OMAiDETQALC.
    OrthoDBiEOG622PMP.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_01694. BioB.
    InterProiIPR013785. Aldolase_TIM.
    IPR010722. BATS_dom.
    IPR002684. Biotin_synth/BioAB.
    IPR024177. Biotin_synthase.
    IPR006638. Elp3/MiaB/NifB.
    IPR007197. rSAM.
    [Graphical view]
    PfamiPF06968. BATS. 1 hit.
    PF04055. Radical_SAM. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001619. Biotin_synth. 1 hit.
    SMARTiSM00876. BATS. 1 hit.
    SM00729. Elp3. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00433. bioB. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q82SL7-1 [UniParc]FASTAAdd to Basket

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    MESATSLISE KQCECAHPNS DSAVQGSSLR WSIAAIESLL DLPFSDLIFQ    50
    AQTVHRQYHD ANAVQLSTLI SVKTGGCSED CAYCPQAARY HTGVENQAIL 100
    SREEVVAAAT QAKESGATRF CMGAAWRGPK QRDIEYMTEV ISAVKALGME 150
    TCATLGILKP GQATQLKQAG LDYYNHNLDT APEFYGEIIT TREYQDRLDT 200
    LEEVRGANIN VCCGGIVGLG ESRMARAGLI AQLANLDPYP ESVPINYLVQ 250
    VEGTPLYGTP ELDPFEFVRT IAAARITMPK AMVRLSAGRR QMPEAIQALC 300
    FLAGANSIFY GDKLLTTGNP DTEKDLALFE KLGLHAL 337
    Length:337
    Mass (Da):36,604
    Last modified:July 28, 2009 - v2
    Checksum:i420CF95A0FD491FB
    GO

    Sequence cautioni

    The sequence CAD86212.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL954747 Genomic DNA. Translation: CAD86212.1. Different initiation.
    RefSeqiNP_842297.1. NC_004757.1.

    Genome annotation databases

    EnsemblBacteriaiCAD86212; CAD86212; NE2300.
    GeneIDi1083267.
    KEGGineu:NE2300.
    PATRICi22716002. VBINitEur56163_2578.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL954747 Genomic DNA. Translation: CAD86212.1 . Different initiation.
    RefSeqi NP_842297.1. NC_004757.1.

    3D structure databases

    ProteinModelPortali Q82SL7.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 228410.NE2300.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAD86212 ; CAD86212 ; NE2300 .
    GeneIDi 1083267.
    KEGGi neu:NE2300.
    PATRICi 22716002. VBINitEur56163_2578.

    Phylogenomic databases

    eggNOGi COG0502.
    HOGENOMi HOG000239957.
    KOi K01012.
    OMAi DETQALC.
    OrthoDBi EOG622PMP.

    Enzyme and pathway databases

    UniPathwayi UPA00078 ; UER00162 .
    BioCyci NEUR228410:GJNO-2345-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_01694. BioB.
    InterProi IPR013785. Aldolase_TIM.
    IPR010722. BATS_dom.
    IPR002684. Biotin_synth/BioAB.
    IPR024177. Biotin_synthase.
    IPR006638. Elp3/MiaB/NifB.
    IPR007197. rSAM.
    [Graphical view ]
    Pfami PF06968. BATS. 1 hit.
    PF04055. Radical_SAM. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001619. Biotin_synth. 1 hit.
    SMARTi SM00876. BATS. 1 hit.
    SM00729. Elp3. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00433. bioB. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Complete genome sequence of the ammonia-oxidizing bacterium and obligate chemolithoautotroph Nitrosomonas europaea."
      Chain P., Lamerdin J.E., Larimer F.W., Regala W., Lao V., Land M.L., Hauser L., Hooper A.B., Klotz M.G., Norton J., Sayavedra-Soto L.A., Arciero D.M., Hommes N.G., Whittaker M.M., Arp D.J.
      J. Bacteriol. 185:2759-2773(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 19718 / NBRC 14298.

    Entry informationi

    Entry nameiBIOB_NITEU
    AccessioniPrimary (citable) accession number: Q82SL7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 28, 2009
    Last sequence update: July 28, 2009
    Last modified: October 1, 2014
    This is version 77 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3