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Q82SL7 (BIOB_NITEU) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 76. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Biotin synthase

EC=2.8.1.6
Gene names
Name:bioB
Ordered Locus Names:NE2300
OrganismNitrosomonas europaea (strain ATCC 19718 / NBRC 14298) [Complete proteome] [HAMAP]
Taxonomic identifier228410 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaNitrosomonadalesNitrosomonadaceaeNitrosomonas

Protein attributes

Sequence length337 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity. HAMAP-Rule MF_01694

Catalytic activity

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_01694

Cofactor

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathway

Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2. HAMAP-Rule MF_01694

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01694

Sequence similarities

Belongs to the radical SAM superfamily. Biotin synthase family.

Sequence caution

The sequence CAD86212.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 337337Biotin synthase HAMAP-Rule MF_01694
PRO_0000381501

Sites

Metal binding771Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding811Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding841Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding1211Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1521Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2121Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2841Iron-sulfur 2 (2Fe-2S) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q82SL7 [UniParc].

Last modified July 28, 2009. Version 2.
Checksum: 420CF95A0FD491FB

FASTA33736,604
        10         20         30         40         50         60 
MESATSLISE KQCECAHPNS DSAVQGSSLR WSIAAIESLL DLPFSDLIFQ AQTVHRQYHD 

        70         80         90        100        110        120 
ANAVQLSTLI SVKTGGCSED CAYCPQAARY HTGVENQAIL SREEVVAAAT QAKESGATRF 

       130        140        150        160        170        180 
CMGAAWRGPK QRDIEYMTEV ISAVKALGME TCATLGILKP GQATQLKQAG LDYYNHNLDT 

       190        200        210        220        230        240 
APEFYGEIIT TREYQDRLDT LEEVRGANIN VCCGGIVGLG ESRMARAGLI AQLANLDPYP 

       250        260        270        280        290        300 
ESVPINYLVQ VEGTPLYGTP ELDPFEFVRT IAAARITMPK AMVRLSAGRR QMPEAIQALC 

       310        320        330 
FLAGANSIFY GDKLLTTGNP DTEKDLALFE KLGLHAL 

« Hide

References

[1]"Complete genome sequence of the ammonia-oxidizing bacterium and obligate chemolithoautotroph Nitrosomonas europaea."
Chain P., Lamerdin J.E., Larimer F.W., Regala W., Lao V., Land M.L., Hauser L., Hooper A.B., Klotz M.G., Norton J., Sayavedra-Soto L.A., Arciero D.M., Hommes N.G., Whittaker M.M., Arp D.J.
J. Bacteriol. 185:2759-2773(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 19718 / NBRC 14298.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AL954747 Genomic DNA. Translation: CAD86212.1. Different initiation.
RefSeqNP_842297.1. NC_004757.1.

3D structure databases

ProteinModelPortalQ82SL7.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING228410.NE2300.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAD86212; CAD86212; NE2300.
GeneID1083267.
KEGGneu:NE2300.
PATRIC22716002. VBINitEur56163_2578.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0502.
HOGENOMHOG000239957.
KOK01012.
OMADETQALC.
OrthoDBEOG622PMP.

Enzyme and pathway databases

BioCycNEUR228410:GJNO-2345-MONOMER.
UniPathwayUPA00078; UER00162.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_01694. BioB.
InterProIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFPIRSF001619. Biotin_synth. 1 hit.
SMARTSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00433. bioB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBIOB_NITEU
AccessionPrimary (citable) accession number: Q82SL7
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: July 28, 2009
Last modified: May 14, 2014
This is version 76 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways