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Q82SJ8 (NAGZ_NITEU) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-hexosaminidase

EC=3.2.1.52
Alternative name(s):
Beta-N-acetylhexosaminidase
N-acetyl-beta-glucosaminidase
Gene names
Name:nagZ
Ordered Locus Names:NE2320
OrganismNitrosomonas europaea (strain ATCC 19718 / NBRC 14298) [Complete proteome] [HAMAP]
Taxonomic identifier228410 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaNitrosomonadalesNitrosomonadaceaeNitrosomonas

Protein attributes

Sequence length348 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Plays a role in peptidoglycan recycling by cleaving the terminal beta-1,4-linked N-acetylglucosamine (GlcNAc) from peptide-linked peptidoglycan fragments, giving rise to free GlcNAc, anhydro-N-acetylmuramic acid and anhydro-N-acetylmuramic acid-linked peptides By similarity. HAMAP-Rule MF_00364

Catalytic activity

Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides. HAMAP-Rule MF_00364

Pathway

Cell wall biogenesis; peptidoglycan recycling. HAMAP-Rule MF_00364

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00364.

Sequence similarities

Belongs to the glycosyl hydrolase 3 family. NagZ subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 348348Beta-hexosaminidase HAMAP-Rule MF_00364
PRO_0000210792

Regions

Region168 – 1692Substrate binding By similarity

Sites

Active site1811Proton donor/acceptor By similarity
Active site2521Nucleophile By similarity
Binding site641Substrate By similarity
Binding site721Substrate By similarity
Binding site1381Substrate By similarity
Site1791Important for catalytic activity By similarity

Sequences

Sequence LengthMass (Da)Tools
Q82SJ8 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: 5167C65A2B3CCD8C

FASTA34837,871
        10         20         30         40         50         60 
MSLGPLMLDI AGTELTETDR VRLSHPLVGG VILFARNYAS PAQLAGLTAE IHALRYPSLL 

        70         80         90        100        110        120 
IAVDQEGGRV QRFRDGFARL PPMRVLGEIC DRNPDRAHHL ASQAGYVLAA ELKACGVDLS 

       130        140        150        160        170        180 
FTPVLDLDYG QSCVIGDRAF HREPQVVADL ACALMNGLQS AGMAAVGKHF PGHGAIRADT 

       190        200        210        220        230        240 
HVETAIDTRS YTDIEKEDLI PFRRMIDAGL SGIMAAHVIY PAIDQHSAGF SSRWLQRILR 

       250        260        270        280        290        300 
HDLGFEGCIF SDDLGMQAAR NYGSITRRAE QALQAGCDMV LVCNDADAAD ELLGSLHWEF 

       310        320        330        340 
SAASLARVEC MRGQHMIHSM AQLHEMERFL QAADEISKID PAGISVSL 

« Hide

References

[1]"Complete genome sequence of the ammonia-oxidizing bacterium and obligate chemolithoautotroph Nitrosomonas europaea."
Chain P., Lamerdin J.E., Larimer F.W., Regala W., Lao V., Land M.L., Hauser L., Hooper A.B., Klotz M.G., Norton J., Sayavedra-Soto L.A., Arciero D.M., Hommes N.G., Whittaker M.M., Arp D.J.
J. Bacteriol. 185:2759-2773(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 19718 / NBRC 14298.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AL954747 Genomic DNA. Translation: CAD86232.1.
RefSeqNP_842317.1. NC_004757.1.

3D structure databases

ProteinModelPortalQ82SJ8.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING228410.NE2320.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAD86232; CAD86232; NE2320.
GeneID1083287.
KEGGneu:NE2320.
PATRIC22716046. VBINitEur56163_2600.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1472.
HOGENOMHOG000248526.
KOK01207.
OMAHGVFSAI.
OrthoDBEOG6BCT06.
PhylomeDBQ82SJ8.

Enzyme and pathway databases

BioCycNEUR228410:GJNO-2365-MONOMER.
UniPathwayUPA00544.

Family and domain databases

Gene3D3.20.20.300. 1 hit.
HAMAPMF_00364. NagZ.
InterProIPR022956. Beta_hexosaminidase_bac.
IPR019800. Glyco_hydro_3_AS.
IPR001764. Glyco_hydro_3_N.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamPF00933. Glyco_hydro_3. 1 hit.
[Graphical view]
SUPFAMSSF51445. SSF51445. 1 hit.
PROSITEPS00775. GLYCOSYL_HYDROL_F3. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNAGZ_NITEU
AccessionPrimary (citable) accession number: Q82SJ8
Entry history
Integrated into UniProtKB/Swiss-Prot: May 24, 2004
Last sequence update: June 1, 2003
Last modified: July 9, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries